AIM:To investigate the effects of growth inhibition ofhuman gastric cancer SGC-7901 cell with RRR-α-tocopherylsuccinate(VES),a derivative of natural Vitamin E,viainducing apoptosis and DNA synthesis arrest.METHODS:Hu...AIM:To investigate the effects of growth inhibition ofhuman gastric cancer SGC-7901 cell with RRR-α-tocopherylsuccinate(VES),a derivative of natural Vitamin E,viainducing apoptosis and DNA synthesis arrest.METHODS:Human gastric cancer SGC-7901 cells wereregularly incubated in the presence of VES at 5,10 and20mg.L^(-1)(VES was dissolved in absolute ethanol anddiluted in RPMI 1640 complete condition mediacorrespondingly to a final concentration of VES and lmL.L^(-1)ethanol),succinic acid and ethanol equivalents asvehicle(VEH)control and condition-media only asuntreated(UT)control.Trypan blue dye exclusionanalysis and MTT assay were applied to detect the cellproliferation.37kBq of tritiated thymidine was added tocells and [~3H]TdR uptake was measured to observe DNAsynthesis.Apoptotic morphology was observed byelectron microscopy and DAPI staining.Flow cytometryand terminal deoxynucleotidyl transferase-mediated dUTPnick end labeling(TUNEL)assay were performed to detectVES-triggered apoptosis.RESULTS:VES inhibited SGC-7901 cell growth in a dose-dependent manner.The growth curve showed suppressionby 24.7%,49.2% and 68.7% following 24h of VEStreatment at 5,10 and 20 mg·L^(-1),respectively,similar tothe findings from MTT assay.DNA synthesis wasevidently reduced by 35%,45% and 98% after 24h VEStreatment at 20 mg·L^(-1)and 48h at 10 and 20 mg·L^(-1),respectively.VES induced SGC-7901 cells to undergoapoptosis with typically apoptotic characteristics,including morphological changes of.chromatincondensation,chromatincrescent formation/margination,nucleus fragmentation and apoptotic body formation,typical apoptotic sub-G1 peak by flow cytometry andincrease of apoptotic cells by TUNEL assay in which 90%of cells underwent apoptosis after 48h of VES treatment at20 mg·L^(-1).CONCLUSION:VES can inhibit human gastric cancer SGC-7901 cell growth by inducing apoptosis and DNA synthesisarrest,inhibition of SGC-7901 cell growth by VES is dose-and time-dependent.Therefore VES can function as apotent chemotherapeutic agent against human gastric carcinogenesis.展开更多
AIM: To explore the effect of Sinai san decoction on the development of non-alcoholic steatohepatitis induced by CCL4 combined with a fat-rich diet in rats.METHODS: Twenty-seven Sprague-Dawley rats were divided into t...AIM: To explore the effect of Sinai san decoction on the development of non-alcoholic steatohepatitis induced by CCL4 combined with a fat-rich diet in rats.METHODS: Twenty-seven Sprague-Dawley rats were divided into three groups randomly: control group (n = 9),model group (n = 9) and treatment group (n = 9). The rats of model group and treatment group were given small dosage of CCL4 combined with a fat-rich diet, andthose of control group were given normal diet. After four weeks of fat-rich diet feeding, the rats of treatment group were given Sinai san decoction. The serum levels of aminotransferase and lipid were measured, and the pathology of livers was observed by HE staining after the rats were sacrificed at eight weeks.RESULTS: The rats' livers presented the pathology of steatosis and inflammation with higher serum levels of ALT and AST in the model group. In the treatment group the serum ALT and AST levels decreased significantly and were close to the control group. The hepatic inflammation scores also decreased markedly, but were still higher than those of control group. And the degree of hepatocyte steatosis was similar to that of model group.CONCLUSION: Sinai san decoction may ameliorate the hepatic inflammation of rats with steatohepatitis induced by small dosage of CCL4 combined with a fat-rich diet,but does not prevent the development of hepatocyte steatosis.展开更多
AIM:To investigate the roles of c-Jun N-terminal kinase (JNK) signaling pathway in vitamin E succinate-induced apoptosis in human gastric cancer SGC-7901 cells. METHODS:Human gastric cancer cell lines (SGC-7901) were ...AIM:To investigate the roles of c-Jun N-terminal kinase (JNK) signaling pathway in vitamin E succinate-induced apoptosis in human gastric cancer SGC-7901 cells. METHODS:Human gastric cancer cell lines (SGC-7901) were treated with vitamin E succinate (VES) at 5,10,20 mg/L. Succinic acid and vitamin E were used as vehicle controls and condition medium only as an untreated (UT) control. Apoptosis was observed by 4′,6-diamidine-2′-phenylindole dihydrochloride (DAPI) staining for morphological changes and by DNA fragmentation for biochemical alterations. Western blot analysis was applied to measure the expression of JNK and phosphorylated JNK.After the cells were transiently transfected with dominant negative mutant of JNK (DN- JNK) followed by treatment of VES,the expression of JNK and c-Jun protein was determined. RESULTS:The apoptotic changes were observed after VES treatment by DNA fragmentation.DNA ladder in the 20 mg/L VES group was more clearly seen than that in 10 mg/L VES group and was not detected following treatment of UT control,succinate and vitamin E.VES at 5,10 and 20 mg/L increased the expression of p-JNK by 2.5-,2.8- and 4.2- fold,respectively.VES induced the phosphorylation of JNK beginning at 1.5 h and produced a sustained increase for 24 h with the peak level at 12 h.Transient transfection of DN-JNK blocked VES-triggered apoptosis by 52%.DN-JNK significantly increased the level of JNK,while decreasing the expression of VES-induced c-Jun protein. CONCLUSION:VES-induced apoptosis in human gastric cancer SGC-7901 cells involves JNK signaling pathway via c-Jun and its downstream transcription factor.展开更多
AIM: To investigate the early diagnostic methods of bacterial and fungal infection in patients with chronic cholestatic hepatitis B.METHODS: One hundred and one adult in-patients with chronic hepatitis B were studied ...AIM: To investigate the early diagnostic methods of bacterial and fungal infection in patients with chronic cholestatic hepatitis B.METHODS: One hundred and one adult in-patients with chronic hepatitis B were studied and divided into 3 groups:direct bilirubin (DBil)/total bilirubin (TBil)≥0.5, without bacterial and fungal infection (group A, n=-38); DBil/TBil<0.5, without bacterial and fungal infection (group B, n=23),DBil/TBil≥0.5, with bacterial or fungal infection (group C,rr=-40). The serum biochemical index and pulse rate were analyzed.RESULTS: Level of TBil, DBil, alkaline phosphatase (ALP) and DBiI/ALP in group A increased compared with that in group B. The level of ALP in group C decreased compared with that in group A, whereas the level of TBil, DBil and DBiI/ALP increased (ALP: 156+43, 199+68, respectively,P<0.05, TBil: 370+227, 220+206, respectively, P<0.01,DBil: 214+143, 146+136, respectively, P<0.01, DBiI/ALP:1.65+1.05, 0.78+0.70, respectively, P<0.001). The level of DBil and infection affected DBiI/ALP. Independent of theeffect of DBil, infection caused DBiI/ALP to rise (P<0.05).The pulse rate in group A decreased compared with that in group B (63.7+6.4, 77.7+11.4, respectively, P<0.001),and the pulse rate in group C increased compared withthat in group A (81.2+12.2, 63.7+6.4, respectively, P<0.001).The equation (infection=0.218 pusle rate +1.064 DBiI/ALP-16.361), with total accuracy of 85.5%, was obtained from stepwise logistic regression. Pulse rate (≥80/min) andDBiI/ALP (≥1.0) were used to screen infection. The sensitivity was 62.5% and 64.7% respectively, and the specificity was 100% and 82.8% respectively.CONCLUSION: Bacterial and fungal infection deterioratejaundice and increase pulse rate, decrease serum ALP andincrease DBiI/ALP. Pulse rate, DBiI/ALP and the equation(infection=0.218 pusle rate+1.064 DBil/ALP-16.361) arehelpful to early diagnosis of bacterial and fungal infectionin patients with chronic cholestatic hepatitis B.展开更多
Pulsed Nd:YAG laser was used to irradiate Si substrate immersed in AgNO3 ethylene glycol solution to deposit Ag films along the lines scanned by laser on the substrate, which is a photo-thermal decomposing process. Th...Pulsed Nd:YAG laser was used to irradiate Si substrate immersed in AgNO3 ethylene glycol solution to deposit Ag films along the lines scanned by laser on the substrate, which is a photo-thermal decomposing process. The decomposed Ag atoms congregate and form polycrystalline Ag particles. The Ag concentration changes greatly with the total laser energyA absorbed by substrate. Transmission electron microscopy (TEM) observation shows the Ag particles are inlaid in the Si substrate. Auger electron spectrum (AES) shows that the Ag concentration decreases with the increase of the sputtering depth, and there is no oxygen element on the surface of the deposited Ag films.展开更多
基金National Natural Science Foundation of China,No.39870662
文摘AIM:To investigate the effects of growth inhibition ofhuman gastric cancer SGC-7901 cell with RRR-α-tocopherylsuccinate(VES),a derivative of natural Vitamin E,viainducing apoptosis and DNA synthesis arrest.METHODS:Human gastric cancer SGC-7901 cells wereregularly incubated in the presence of VES at 5,10 and20mg.L^(-1)(VES was dissolved in absolute ethanol anddiluted in RPMI 1640 complete condition mediacorrespondingly to a final concentration of VES and lmL.L^(-1)ethanol),succinic acid and ethanol equivalents asvehicle(VEH)control and condition-media only asuntreated(UT)control.Trypan blue dye exclusionanalysis and MTT assay were applied to detect the cellproliferation.37kBq of tritiated thymidine was added tocells and [~3H]TdR uptake was measured to observe DNAsynthesis.Apoptotic morphology was observed byelectron microscopy and DAPI staining.Flow cytometryand terminal deoxynucleotidyl transferase-mediated dUTPnick end labeling(TUNEL)assay were performed to detectVES-triggered apoptosis.RESULTS:VES inhibited SGC-7901 cell growth in a dose-dependent manner.The growth curve showed suppressionby 24.7%,49.2% and 68.7% following 24h of VEStreatment at 5,10 and 20 mg·L^(-1),respectively,similar tothe findings from MTT assay.DNA synthesis wasevidently reduced by 35%,45% and 98% after 24h VEStreatment at 20 mg·L^(-1)and 48h at 10 and 20 mg·L^(-1),respectively.VES induced SGC-7901 cells to undergoapoptosis with typically apoptotic characteristics,including morphological changes of.chromatincondensation,chromatincrescent formation/margination,nucleus fragmentation and apoptotic body formation,typical apoptotic sub-G1 peak by flow cytometry andincrease of apoptotic cells by TUNEL assay in which 90%of cells underwent apoptosis after 48h of VES treatment at20 mg·L^(-1).CONCLUSION:VES can inhibit human gastric cancer SGC-7901 cell growth by inducing apoptosis and DNA synthesisarrest,inhibition of SGC-7901 cell growth by VES is dose-and time-dependent.Therefore VES can function as apotent chemotherapeutic agent against human gastric carcinogenesis.
文摘AIM: To explore the effect of Sinai san decoction on the development of non-alcoholic steatohepatitis induced by CCL4 combined with a fat-rich diet in rats.METHODS: Twenty-seven Sprague-Dawley rats were divided into three groups randomly: control group (n = 9),model group (n = 9) and treatment group (n = 9). The rats of model group and treatment group were given small dosage of CCL4 combined with a fat-rich diet, andthose of control group were given normal diet. After four weeks of fat-rich diet feeding, the rats of treatment group were given Sinai san decoction. The serum levels of aminotransferase and lipid were measured, and the pathology of livers was observed by HE staining after the rats were sacrificed at eight weeks.RESULTS: The rats' livers presented the pathology of steatosis and inflammation with higher serum levels of ALT and AST in the model group. In the treatment group the serum ALT and AST levels decreased significantly and were close to the control group. The hepatic inflammation scores also decreased markedly, but were still higher than those of control group. And the degree of hepatocyte steatosis was similar to that of model group.CONCLUSION: Sinai san decoction may ameliorate the hepatic inflammation of rats with steatohepatitis induced by small dosage of CCL4 combined with a fat-rich diet,but does not prevent the development of hepatocyte steatosis.
基金Supported by National Natural Science Foundation of China,No.39870662
文摘AIM:To investigate the roles of c-Jun N-terminal kinase (JNK) signaling pathway in vitamin E succinate-induced apoptosis in human gastric cancer SGC-7901 cells. METHODS:Human gastric cancer cell lines (SGC-7901) were treated with vitamin E succinate (VES) at 5,10,20 mg/L. Succinic acid and vitamin E were used as vehicle controls and condition medium only as an untreated (UT) control. Apoptosis was observed by 4′,6-diamidine-2′-phenylindole dihydrochloride (DAPI) staining for morphological changes and by DNA fragmentation for biochemical alterations. Western blot analysis was applied to measure the expression of JNK and phosphorylated JNK.After the cells were transiently transfected with dominant negative mutant of JNK (DN- JNK) followed by treatment of VES,the expression of JNK and c-Jun protein was determined. RESULTS:The apoptotic changes were observed after VES treatment by DNA fragmentation.DNA ladder in the 20 mg/L VES group was more clearly seen than that in 10 mg/L VES group and was not detected following treatment of UT control,succinate and vitamin E.VES at 5,10 and 20 mg/L increased the expression of p-JNK by 2.5-,2.8- and 4.2- fold,respectively.VES induced the phosphorylation of JNK beginning at 1.5 h and produced a sustained increase for 24 h with the peak level at 12 h.Transient transfection of DN-JNK blocked VES-triggered apoptosis by 52%.DN-JNK significantly increased the level of JNK,while decreasing the expression of VES-induced c-Jun protein. CONCLUSION:VES-induced apoptosis in human gastric cancer SGC-7901 cells involves JNK signaling pathway via c-Jun and its downstream transcription factor.
文摘AIM: To investigate the early diagnostic methods of bacterial and fungal infection in patients with chronic cholestatic hepatitis B.METHODS: One hundred and one adult in-patients with chronic hepatitis B were studied and divided into 3 groups:direct bilirubin (DBil)/total bilirubin (TBil)≥0.5, without bacterial and fungal infection (group A, n=-38); DBil/TBil<0.5, without bacterial and fungal infection (group B, n=23),DBil/TBil≥0.5, with bacterial or fungal infection (group C,rr=-40). The serum biochemical index and pulse rate were analyzed.RESULTS: Level of TBil, DBil, alkaline phosphatase (ALP) and DBiI/ALP in group A increased compared with that in group B. The level of ALP in group C decreased compared with that in group A, whereas the level of TBil, DBil and DBiI/ALP increased (ALP: 156+43, 199+68, respectively,P<0.05, TBil: 370+227, 220+206, respectively, P<0.01,DBil: 214+143, 146+136, respectively, P<0.01, DBiI/ALP:1.65+1.05, 0.78+0.70, respectively, P<0.001). The level of DBil and infection affected DBiI/ALP. Independent of theeffect of DBil, infection caused DBiI/ALP to rise (P<0.05).The pulse rate in group A decreased compared with that in group B (63.7+6.4, 77.7+11.4, respectively, P<0.001),and the pulse rate in group C increased compared withthat in group A (81.2+12.2, 63.7+6.4, respectively, P<0.001).The equation (infection=0.218 pusle rate +1.064 DBiI/ALP-16.361), with total accuracy of 85.5%, was obtained from stepwise logistic regression. Pulse rate (≥80/min) andDBiI/ALP (≥1.0) were used to screen infection. The sensitivity was 62.5% and 64.7% respectively, and the specificity was 100% and 82.8% respectively.CONCLUSION: Bacterial and fungal infection deterioratejaundice and increase pulse rate, decrease serum ALP andincrease DBiI/ALP. Pulse rate, DBiI/ALP and the equation(infection=0.218 pusle rate+1.064 DBil/ALP-16.361) arehelpful to early diagnosis of bacterial and fungal infectionin patients with chronic cholestatic hepatitis B.
基金This work was financially supported by the special funds for the major basic research projects(No.G2000067205-4).
文摘Pulsed Nd:YAG laser was used to irradiate Si substrate immersed in AgNO3 ethylene glycol solution to deposit Ag films along the lines scanned by laser on the substrate, which is a photo-thermal decomposing process. The decomposed Ag atoms congregate and form polycrystalline Ag particles. The Ag concentration changes greatly with the total laser energyA absorbed by substrate. Transmission electron microscopy (TEM) observation shows the Ag particles are inlaid in the Si substrate. Auger electron spectrum (AES) shows that the Ag concentration decreases with the increase of the sputtering depth, and there is no oxygen element on the surface of the deposited Ag films.
