Non-alcoholic fatty liver disease(NAFLD)is prevalent worldwide as a chronic liver disease that not only gives rise to hepatic complications,but leads to other chronic diseases such as type 2 diabetes and atheroscleros...Non-alcoholic fatty liver disease(NAFLD)is prevalent worldwide as a chronic liver disease that not only gives rise to hepatic complications,but leads to other chronic diseases such as type 2 diabetes and atherosclerosis.The aberrant accumulation of lipid droplets(LDs)in hepatocytes is a prominent signature of NAFLD.However,conventional techniques lack the capability to effectively monitor the dynamic changes in LD levels during NAFLD with living organisms.Hence,it is imperative to develop LD-specific long-wavelength fluorescent probes with high imaging contrast for the in-situ diagnosis of NAFLD.In this study,we synthesized a new LD-selective long-wavelength fluorescent probe,denoted as LD-1,based on the twisted intramolecular charge transfer(TICT)mechanism.The probe exhibits a large Stokes shift and intensive fluorescence emission in nonpolar and viscous solutions.By self-assembling LD-1 with bovine serum albumin(BSA),a biocompatible,long-wavelength fluorescent probe hybrid,LD-1@BSA,was formed,allowing for LDs to be selectively imaged in hepatocytes.Moreover,LD-1@BSA successfully discriminates NAFLD cells before and after drug treatment,and achieves non-invasive and real-time monitoring of LD accumulation in a mouse model of NAFLD.展开更多
Multiple myeloma(MM)is a plasmocytic malignancy which accounts for approximately 10%of all hematologic malignancies.In 2016,over 16,500 new cases were diagnosed in China,and approximately 10,300 patients died of the d...Multiple myeloma(MM)is a plasmocytic malignancy which accounts for approximately 10%of all hematologic malignancies.In 2016,over 16,500 new cases were diagnosed in China,and approximately 10,300 patients died of the disease.[1]In novel-agent era,some patients had a poor prognosis with a median overall survival(OS)of<2 years although they received the therapy regimen containing novel agents and autologous peripheral blood stem cell transplantation(APBSCT)as front-line therapy.Stratification for Myeloma and Risk-Adapted Therapy 3.0(mSMART 3.0)from Mayo Clinic is a risk-stratification guideline revised in 2020.[2]This risk-stratification guideline proposed a novel point of view as having two of the above genetic abnormalities to be named as doublehit MM and having any three or more as triple-hit MM.展开更多
基金National Natural Science Foundation of China(Nos.22107029,22377135,22305178,92253306 and 82130099)the Taishan Scholars Program(No.tsqn202312305)+7 种基金the Young Elite Scientists Sponsorship Program by Chinese Chemical Societythe Fundamental Research Projects of Science&Technology Innovation and development Plan in Yantai City(No.2023JCYJ059)the Shandong Laboratory Program(No.SYS202205)the Fundamental Research Funds for the Central Universities(No.222201717003)the Programme of Introducing Talents of Discipline to Universities(No.B16017)the Open Funding Project of the State Key Laboratory of Bioreactor Engineeringthe State Key Laboratory of Drug Research(No.SKLDR-2023-KF-10)the Ministry of Education Key Laboratory on signaling Regulation and Targeting Therapy of Liver Cancer(Naval Medical University)(No.2023-MEKLLCMS/ZD-00*)。
文摘Non-alcoholic fatty liver disease(NAFLD)is prevalent worldwide as a chronic liver disease that not only gives rise to hepatic complications,but leads to other chronic diseases such as type 2 diabetes and atherosclerosis.The aberrant accumulation of lipid droplets(LDs)in hepatocytes is a prominent signature of NAFLD.However,conventional techniques lack the capability to effectively monitor the dynamic changes in LD levels during NAFLD with living organisms.Hence,it is imperative to develop LD-specific long-wavelength fluorescent probes with high imaging contrast for the in-situ diagnosis of NAFLD.In this study,we synthesized a new LD-selective long-wavelength fluorescent probe,denoted as LD-1,based on the twisted intramolecular charge transfer(TICT)mechanism.The probe exhibits a large Stokes shift and intensive fluorescence emission in nonpolar and viscous solutions.By self-assembling LD-1 with bovine serum albumin(BSA),a biocompatible,long-wavelength fluorescent probe hybrid,LD-1@BSA,was formed,allowing for LDs to be selectively imaged in hepatocytes.Moreover,LD-1@BSA successfully discriminates NAFLD cells before and after drug treatment,and achieves non-invasive and real-time monitoring of LD accumulation in a mouse model of NAFLD.
基金the Major Special Project of the Ministry of Science and Technology in China(No.2018ZX09733003)Beijing Hospital Authority“Dengfeng”Talent Training Program(No.DFL20180301).
文摘Multiple myeloma(MM)is a plasmocytic malignancy which accounts for approximately 10%of all hematologic malignancies.In 2016,over 16,500 new cases were diagnosed in China,and approximately 10,300 patients died of the disease.[1]In novel-agent era,some patients had a poor prognosis with a median overall survival(OS)of<2 years although they received the therapy regimen containing novel agents and autologous peripheral blood stem cell transplantation(APBSCT)as front-line therapy.Stratification for Myeloma and Risk-Adapted Therapy 3.0(mSMART 3.0)from Mayo Clinic is a risk-stratification guideline revised in 2020.[2]This risk-stratification guideline proposed a novel point of view as having two of the above genetic abnormalities to be named as doublehit MM and having any three or more as triple-hit MM.
