目的本研究旨在评估基于新冠病毒原始毒株与奥密克戎(Omicron)变异株开发的SARS-CoV-2抗体检测试剂,在未接种疫苗的艾滋病病毒感染者(people living with HIV,PLWH)感染Omicron变异株后的检测性能。方法本研究招募了45名未接种新冠疫苗...目的本研究旨在评估基于新冠病毒原始毒株与奥密克戎(Omicron)变异株开发的SARS-CoV-2抗体检测试剂,在未接种疫苗的艾滋病病毒感染者(people living with HIV,PLWH)感染Omicron变异株后的检测性能。方法本研究招募了45名未接种新冠疫苗、首次感染Omicron BA.5毒株的PLWH,并在4个月后进行随访。使用八种针对原始毒株和两种针对Omicron毒株的抗体试剂检测抗体水平,并以针对原始毒株(wild-type,WT)、BA.4/5和XBB.1.5的假病毒中和实验为金标准,使用McNemar与Kappa检验定性结果的一致性,使用斯皮尔曼(Spearman)相关性分析定量结果的相关性。结果原始毒株抗体试剂与WT中和抗体(neutralizing antibody,nAb)的一致性最高(κ值最高为0.483),与Omicron-nAb的一致性明显较低。在定量分析中,原始毒株抗体试剂与WT-nAb呈强相关(r_(s)=0.69~0.85),而与BA.4/5(r_(s)=0.40~0.57)和XBB.1.5(r_(s)=0.43~0.63)-nAb的相关性较弱。二次感染后,原始毒株抗体试剂与Omicron-nAb之间的相关性有所提升。Omicron特异性抗体试剂与Omicron-nAb的95%一致性界限更窄。结论在未接种疫苗的PLWH中,原始毒株抗体试剂对Omicron感染后的免疫应答检测存在局限性,研究结果支持开发和应用变异株特异性抗体试剂,以实现更准确的免疫监测和评估。展开更多
AIM:To investigate the underlying causes of surgical failure and reoperation management in patients with rhegmatogenous retinal detachment(RRD)who underwent scleral buckle surgery at our institution.METHODS:This was a...AIM:To investigate the underlying causes of surgical failure and reoperation management in patients with rhegmatogenous retinal detachment(RRD)who underwent scleral buckle surgery at our institution.METHODS:This was a single-center,retrospective,descriptive study.The clinical data of 368 patients(387 eyes)with RRD who underwent scleral buckling(SB)surgery between August 2013 and July 2023 at our institution were collected.The aim was to analyze the causes of recurrence and the rationale for selecting reoperation methods.RESULTS:Totally 368 patients(387 eyes)were included in the analysis,comprising 222 males and 146 females.The average age was 30.26±14.18 years,and the mean follow-up duration was(48.33±20.39)mo.The success rate of SB surgery was 90.2%.Recurrent retinal detachment occurred in 38 eyes.Based on surgical records,the causes of SB failure were analyzed.The recurrence causes included abnormal compression ridge position(position,height,or width)in 14 eyes(36.8%,14/38),hole omission in 11 eyes(29.0%,11/38),proliferative vitreoretinopathy(PVR)in 10 eyes(26.3%,10/38),and new holes in 3 eyes(7.9%,3/38).Among these,8 eyes(21.1%,8/38)underwent repeat SB surgery,while the remaining 30 eyes(78.9%,30/38)underwent pars plana vitrectomy(PPV).Regarding tamponade agents,silicone oil was used in 11 eyes(36.7%,11/30),C_(3)F_(8) gas in 12 eyes(40.0%,12/30),and sterile air in 7 eyes(23.3%,7/30).CONCLUSION:SB surgery demonstrates a high success rate in the treatment of RRD.However,abnormal compression ridge position,missed holes during surgery,and PVR are the primary causes of SB failure.After addressing the reasons for failure,re-SB surgery or PPV can be effective alternatives.展开更多
文摘目的本研究旨在评估基于新冠病毒原始毒株与奥密克戎(Omicron)变异株开发的SARS-CoV-2抗体检测试剂,在未接种疫苗的艾滋病病毒感染者(people living with HIV,PLWH)感染Omicron变异株后的检测性能。方法本研究招募了45名未接种新冠疫苗、首次感染Omicron BA.5毒株的PLWH,并在4个月后进行随访。使用八种针对原始毒株和两种针对Omicron毒株的抗体试剂检测抗体水平,并以针对原始毒株(wild-type,WT)、BA.4/5和XBB.1.5的假病毒中和实验为金标准,使用McNemar与Kappa检验定性结果的一致性,使用斯皮尔曼(Spearman)相关性分析定量结果的相关性。结果原始毒株抗体试剂与WT中和抗体(neutralizing antibody,nAb)的一致性最高(κ值最高为0.483),与Omicron-nAb的一致性明显较低。在定量分析中,原始毒株抗体试剂与WT-nAb呈强相关(r_(s)=0.69~0.85),而与BA.4/5(r_(s)=0.40~0.57)和XBB.1.5(r_(s)=0.43~0.63)-nAb的相关性较弱。二次感染后,原始毒株抗体试剂与Omicron-nAb之间的相关性有所提升。Omicron特异性抗体试剂与Omicron-nAb的95%一致性界限更窄。结论在未接种疫苗的PLWH中,原始毒株抗体试剂对Omicron感染后的免疫应答检测存在局限性,研究结果支持开发和应用变异株特异性抗体试剂,以实现更准确的免疫监测和评估。
文摘AIM:To investigate the underlying causes of surgical failure and reoperation management in patients with rhegmatogenous retinal detachment(RRD)who underwent scleral buckle surgery at our institution.METHODS:This was a single-center,retrospective,descriptive study.The clinical data of 368 patients(387 eyes)with RRD who underwent scleral buckling(SB)surgery between August 2013 and July 2023 at our institution were collected.The aim was to analyze the causes of recurrence and the rationale for selecting reoperation methods.RESULTS:Totally 368 patients(387 eyes)were included in the analysis,comprising 222 males and 146 females.The average age was 30.26±14.18 years,and the mean follow-up duration was(48.33±20.39)mo.The success rate of SB surgery was 90.2%.Recurrent retinal detachment occurred in 38 eyes.Based on surgical records,the causes of SB failure were analyzed.The recurrence causes included abnormal compression ridge position(position,height,or width)in 14 eyes(36.8%,14/38),hole omission in 11 eyes(29.0%,11/38),proliferative vitreoretinopathy(PVR)in 10 eyes(26.3%,10/38),and new holes in 3 eyes(7.9%,3/38).Among these,8 eyes(21.1%,8/38)underwent repeat SB surgery,while the remaining 30 eyes(78.9%,30/38)underwent pars plana vitrectomy(PPV).Regarding tamponade agents,silicone oil was used in 11 eyes(36.7%,11/30),C_(3)F_(8) gas in 12 eyes(40.0%,12/30),and sterile air in 7 eyes(23.3%,7/30).CONCLUSION:SB surgery demonstrates a high success rate in the treatment of RRD.However,abnormal compression ridge position,missed holes during surgery,and PVR are the primary causes of SB failure.After addressing the reasons for failure,re-SB surgery or PPV can be effective alternatives.
