Background:Several surgeons have described studies of free-tissue transfers using veins instead of arteries.These innovative microsurgical techniques can offer several advantages,such as an easier dissection during fl...Background:Several surgeons have described studies of free-tissue transfers using veins instead of arteries.These innovative microsurgical techniques can offer several advantages,such as an easier dissection during flap harvesting,and represent an alternative during an accidental surgical mistake or development of new surgical procedures.The purpose of this study was to describe and explore different constructs of vascularized lymph node transfer(VLNT)only based on venous blood flow in a mouse model,evaluate their blood flow microcirculation through indocyanine green(ICG)angiography and investigate the lymphatic drainage function and the lymph nodes’structures.Methods:Five types of venous lymph node flaps(LNF)were created and investigated:Types IA,IB,IC,IIA and IIB were developed by ICG intraoperatively(with videos in the article).Seven weeks later,by applying methylene blue,the recanalization of the lymphatic vessels between the LNF and the recipient site was detected.Lymph nodes were collected at the same time and their structures were analyzed by hematoxylin and eosin staining analysis.Results:All of the venous LNFs developed except Type IC.Seven weeks later,methylene blue flowed into Types IA,IB,IIA and IIB from recipient sites.When comparing with arteriovenous lymph node,the medullary sinus was diffusely distributed in venous lymph nodes.The proportion of cells was significantly reduced(p<0.05).The artery diameters were significantly smaller(p<0.05).The veins diameters and lymphatic vessels output in Types IA,IB,IIA and IIB were more dilated(p<0.05).Conclusions:This research demonstrated that Type IA,IB,IIA and IIB venous LNFs can retrogradely receive venous blood supply;they can survive,produce a lymphatic recanalization and integrate with the surrounding tissue,despite lymph node structural changes.Our results will improve the understanding of the survival mechanism of venous LNFs and will help researchers to design new studies or lymphatic models and eventually find an alternative procedure for the surgical treatment of lymphedema.展开更多
Keloids are an abnormal fibroproliferative wound-healing disease with a poorly understood pathogenesis,making it difficult to predict and prevent this disease in clinical settings.Identifying disease-specific signatur...Keloids are an abnormal fibroproliferative wound-healing disease with a poorly understood pathogenesis,making it difficult to predict and prevent this disease in clinical settings.Identifying disease-specific signatures at the molecular and cellular levels in both the blood circulation and primary lesions is urgently needed to develop novel biomarkers for risk assessment and therapeutic targets for recurrence-free treatment.There is mounting evidence of immune cell dysregulation in keloid scarring.In this study,we aimed to profile keloid scar tissues and blood cells and found that downregulation of cytotoxic CD8^(+)T cells is a keloid signature in the peripheral blood and keloid lesions.Single-cell RNA sequencing revealed that the NKG2A/CD94 complex was specifically upregulated,which might contribute to the significant reduction in CTLs within the scar tissue boundary.In addition,the NKG2A/CD94 complex was associated with high serum levels of soluble human leukocyte antigen-E(sHLA-E).We subsequently measured sHLA-E in our hospital-based study cohort,consisting of 104 keloid patients,512 healthy donors,and 100 patients with an interfering disease.The sensitivity and specificity of sHLA-E were 83.69%(87/104)and 92.16%(564/612),respectively,and hypertrophic scars and other unrelated diseases exhibited minimal interference with the test results.Furthermore,intralesional therapy with triamcinolone combined with 5-fluorouracil drastically decreased the sHLA-E levels in keloid patients with better prognostic outcomes,while an incomplete reduction in the sHLA-E levels in patient serum was associated with higher recurrence.sHLA-E may effectively serve as a diagnostic marker for assessing the risk of keloid formation and a prognostic marker for the clinical outcomes of intralesional treatment.展开更多
The peripheral nervous system(PNS)is essential for performing and maintaining various motor and sensory functions.Abnormalities can lead to a series of peripheral neurological conditions,such as paraesthesia,pain,or s...The peripheral nervous system(PNS)is essential for performing and maintaining various motor and sensory functions.Abnormalities can lead to a series of peripheral neurological conditions,such as paraesthesia,pain,or spasms,which are debilitating and lowering the quality of life.Thecurrent guidelines for diagnosis rely predominantly on clinical symptoms resulting from PNS dysfunction,which occur already at an advancedstage.There are currently no effective methods that visually reflect the extent of peripheral neuropathy.In our study,we present a novel in vivoand in situ real-time imaging of peripheral nerves based on the second near-infrared window(NIR-II)fluoresce nee.In NIR-II system,lead sulfidequa ntum dots(PbS Qds)with NIR-II fluoresce nee specifically bound to motor neuro rvspecific protein agrin,acting as image con trast.In micemodel,peripheral nerves were visible as soon as after 2 h post injection.We provide evidenee for the efficacy of this approach,which allows todirectly dem on strate peripheral nerves,their structure,and pote ntial damagesites and degree.Furthermore,our products were of goodbiocompatibility,while the n eural fluoresce nee signal was solid,bright and stable for 4 h in vivo.Thus,overall,our results suggest that NIR-II isan effective new method for direct imaging of peripheral nerves in vivo,opening new horizons on early,improved and more precise,targeteddiag no sis.A resulti ng more rapid installatio n of perso nalized therapy facilitates a better prognosis of clinical peripheral neuropathy.展开更多
The administration time is a critical but long-neglected point in cell therapy based on macrophages because the incorrect time of macrophage administration could result in diverse outcomes regarding the same macrophag...The administration time is a critical but long-neglected point in cell therapy based on macrophages because the incorrect time of macrophage administration could result in diverse outcomes regarding the same macrophage therapy.In this work,the second near-infrared(NIR-II)fluorescence imaging in vivo tracking of M2 macrophages during a pro-healing therapy in the mice model of rotator cuff injury revealed that the behavior of administrated macrophages was influenced by the timing of their administration.The delayed cell therapy(DCT)group had a longer retention time of injected M2 macrophages in the repairing tissue than that in the immediate cell therapy(ICT)group.Both Keller-Segel model and histological analysis further demonstrated that DCT altered the chemotaxis of M2 macrophages and improved the healing outcome of the repaired structure in comparison with ICT.Our results offer a possible explanation of previous conflicting results on reparative cell therapy and provoke reconsideration of the timing of these therapies.展开更多
The degradation of collagen in different body parts is a critical point for designing collagen-based biomedical products.Here,three kinds of collagens labeled by second near-infrared(NIR-II)quantum dots(QDs),including...The degradation of collagen in different body parts is a critical point for designing collagen-based biomedical products.Here,three kinds of collagens labeled by second near-infrared(NIR-II)quantum dots(QDs),including collagen with low crosslinking degree(LC),middle crosslinking degree(MC)and high crosslinking degree(HC),were injected into the subcutaneous tissue,muscle and joints of the mouse model,respectively,in order to investigate the in vivo degradation pattern of collagen by NIR-II live imaging.The results of NIR-II imaging indicated that all tested collagens could be fully degraded after 35 days in the subcutaneous tissue,muscle and joints of the mouse model.However,the average degradation rate of subcutaneous tissue(k=0.13)and muscle(k=0.23)was slower than that of the joints(shoulder:k=0.42,knee:k=0.55).Specifically,the degradation rate of HC(k=0.13)was slower than LC(k=0.30)in muscle,while HC showed the fastest degradation rate in the shoulder and knee joints.In summary,NIR-II imaging could precisely identify the in vivo degradation rate of collagen.Moreover,the degradation rate of collagen was more closely related to the implanted body parts rather than the crosslinking degree of collagen,which was slower in the subcutaneous tissue and muscle compared to the joints in the mouse model.展开更多
This paper proposes a technique of image content authentication based on the Laplacian Pyramid to verify the authenticity of image content.First,the image is decomposed into Laplacian Pyramid before the transformation...This paper proposes a technique of image content authentication based on the Laplacian Pyramid to verify the authenticity of image content.First,the image is decomposed into Laplacian Pyramid before the transformation.Next,the smooth and detail properties of the original image are analyzed according to the Laplacian Pyramid,and the properties are classified and encoded to get the corresponding characteristic values.Then,the signature derived from the encrypted characteristic values is embedded in the original image as a watermark.After the reception,the characteristic values of the received image are compared with the watermark drawn out from the image.The algorithm automatically identifies whether the content is tampered by means of morphologic filtration.The information of tampered location is p resented at the same time.Experimental results show that the proposed authentication algorithm can effectively detect the event and location when the original image content is tampered.Moreover,it can tolerate some distortions produced by compression,filtration and noise degradation.展开更多
基金The national natural science foundation of China(Grant Number:81772098)Clinical Multi-Disciplinary Team Research Program of 9th People’s Hospital,Shanghai Jiao Tong University School of Medicine(Grant Number:2017-1-007)+3 种基金Clinical Research Program of 9th People’s Hospital,Shanghai Jiao Tong University School of Medicine(Grant Number:JYLJ027)Shanghai Municipal Education Commission Gaofeng Clinical Medicine Grant Support(Grant Number:20152227)The national natural science foundation of China(Grant Number:82000456)Scientific research foundation of Shanghai Municipal Commission of Health and Family Planning(Grant Number:20154Y0023).
文摘Background:Several surgeons have described studies of free-tissue transfers using veins instead of arteries.These innovative microsurgical techniques can offer several advantages,such as an easier dissection during flap harvesting,and represent an alternative during an accidental surgical mistake or development of new surgical procedures.The purpose of this study was to describe and explore different constructs of vascularized lymph node transfer(VLNT)only based on venous blood flow in a mouse model,evaluate their blood flow microcirculation through indocyanine green(ICG)angiography and investigate the lymphatic drainage function and the lymph nodes’structures.Methods:Five types of venous lymph node flaps(LNF)were created and investigated:Types IA,IB,IC,IIA and IIB were developed by ICG intraoperatively(with videos in the article).Seven weeks later,by applying methylene blue,the recanalization of the lymphatic vessels between the LNF and the recipient site was detected.Lymph nodes were collected at the same time and their structures were analyzed by hematoxylin and eosin staining analysis.Results:All of the venous LNFs developed except Type IC.Seven weeks later,methylene blue flowed into Types IA,IB,IIA and IIB from recipient sites.When comparing with arteriovenous lymph node,the medullary sinus was diffusely distributed in venous lymph nodes.The proportion of cells was significantly reduced(p<0.05).The artery diameters were significantly smaller(p<0.05).The veins diameters and lymphatic vessels output in Types IA,IB,IIA and IIB were more dilated(p<0.05).Conclusions:This research demonstrated that Type IA,IB,IIA and IIB venous LNFs can retrogradely receive venous blood supply;they can survive,produce a lymphatic recanalization and integrate with the surrounding tissue,despite lymph node structural changes.Our results will improve the understanding of the survival mechanism of venous LNFs and will help researchers to design new studies or lymphatic models and eventually find an alternative procedure for the surgical treatment of lymphedema.
基金This study was funded by the National Natural Science Foundation of China(Nos.81772098,81672247,and 82002064)Shanghai Sailing Program(No.20YF1422700)Shanghai Municipal Education Commission Gaofeng Clinical Medicine Grant Support(No.20152227).
文摘Keloids are an abnormal fibroproliferative wound-healing disease with a poorly understood pathogenesis,making it difficult to predict and prevent this disease in clinical settings.Identifying disease-specific signatures at the molecular and cellular levels in both the blood circulation and primary lesions is urgently needed to develop novel biomarkers for risk assessment and therapeutic targets for recurrence-free treatment.There is mounting evidence of immune cell dysregulation in keloid scarring.In this study,we aimed to profile keloid scar tissues and blood cells and found that downregulation of cytotoxic CD8^(+)T cells is a keloid signature in the peripheral blood and keloid lesions.Single-cell RNA sequencing revealed that the NKG2A/CD94 complex was specifically upregulated,which might contribute to the significant reduction in CTLs within the scar tissue boundary.In addition,the NKG2A/CD94 complex was associated with high serum levels of soluble human leukocyte antigen-E(sHLA-E).We subsequently measured sHLA-E in our hospital-based study cohort,consisting of 104 keloid patients,512 healthy donors,and 100 patients with an interfering disease.The sensitivity and specificity of sHLA-E were 83.69%(87/104)and 92.16%(564/612),respectively,and hypertrophic scars and other unrelated diseases exhibited minimal interference with the test results.Furthermore,intralesional therapy with triamcinolone combined with 5-fluorouracil drastically decreased the sHLA-E levels in keloid patients with better prognostic outcomes,while an incomplete reduction in the sHLA-E levels in patient serum was associated with higher recurrence.sHLA-E may effectively serve as a diagnostic marker for assessing the risk of keloid formation and a prognostic marker for the clinical outcomes of intralesional treatment.
基金This work was supported by National Natural Science Foundation of China(Nos.81672247,81772339,8181101445,81811530750,and 81811530389)Shanghai Rising-Star Project(No.18QB1400500),The Key Clinical Medicine Center of Shanghai(No.2017ZZ01006)+2 种基金Sanming Project of Medicine in Shenzhen(No.SZSM201612078)The Introduction Project of Clinical Medicine Expert Team for Suzhou(No.SZYJTD201714)Development Project of Shanghai Peak Disciplines-Integrative Medicine(No.20180101).
文摘The peripheral nervous system(PNS)is essential for performing and maintaining various motor and sensory functions.Abnormalities can lead to a series of peripheral neurological conditions,such as paraesthesia,pain,or spasms,which are debilitating and lowering the quality of life.Thecurrent guidelines for diagnosis rely predominantly on clinical symptoms resulting from PNS dysfunction,which occur already at an advancedstage.There are currently no effective methods that visually reflect the extent of peripheral neuropathy.In our study,we present a novel in vivoand in situ real-time imaging of peripheral nerves based on the second near-infrared window(NIR-II)fluoresce nee.In NIR-II system,lead sulfidequa ntum dots(PbS Qds)with NIR-II fluoresce nee specifically bound to motor neuro rvspecific protein agrin,acting as image con trast.In micemodel,peripheral nerves were visible as soon as after 2 h post injection.We provide evidenee for the efficacy of this approach,which allows todirectly dem on strate peripheral nerves,their structure,and pote ntial damagesites and degree.Furthermore,our products were of goodbiocompatibility,while the n eural fluoresce nee signal was solid,bright and stable for 4 h in vivo.Thus,overall,our results suggest that NIR-II isan effective new method for direct imaging of peripheral nerves in vivo,opening new horizons on early,improved and more precise,targeteddiag no sis.A resulti ng more rapid installatio n of perso nalized therapy facilitates a better prognosis of clinical peripheral neuropathy.
基金the approval of ethics by Ethics Committee of Fudan University(No.202208005Z)supported by the National Natural Science Foundation of China(Nos.81972129,82072521,82111530200)+1 种基金Shanghai Talent Development Funding Scheme(No.2020080)Shanghai Committee of Science and Technology(Nos.22DZ2204900,23ZR1445700)。
文摘The administration time is a critical but long-neglected point in cell therapy based on macrophages because the incorrect time of macrophage administration could result in diverse outcomes regarding the same macrophage therapy.In this work,the second near-infrared(NIR-II)fluorescence imaging in vivo tracking of M2 macrophages during a pro-healing therapy in the mice model of rotator cuff injury revealed that the behavior of administrated macrophages was influenced by the timing of their administration.The delayed cell therapy(DCT)group had a longer retention time of injected M2 macrophages in the repairing tissue than that in the immediate cell therapy(ICT)group.Both Keller-Segel model and histological analysis further demonstrated that DCT altered the chemotaxis of M2 macrophages and improved the healing outcome of the repaired structure in comparison with ICT.Our results offer a possible explanation of previous conflicting results on reparative cell therapy and provoke reconsideration of the timing of these therapies.
基金supported by National Key R&D Program of China(2021YFA1201303)National Natural Science Foundation of China(82172511,81972121,81972129,82072521,82011530023 and 82111530200)+5 种基金Sanming Project of Medicine in Shenzhen(SZSM201612078)the Introduction Project of Clinical Medicine Expert Team for Suzhou(SZYJTD201714)Shanghai Talent Development Funding Scheme(2020080)Shanghai Sailing Program(21YF1404100 and 22YF1405200)Shanghai Committee of Science and Technology(22DZ2204900)Medical Engineering Joint Fund of Fudan University(YG2022-14).
文摘The degradation of collagen in different body parts is a critical point for designing collagen-based biomedical products.Here,three kinds of collagens labeled by second near-infrared(NIR-II)quantum dots(QDs),including collagen with low crosslinking degree(LC),middle crosslinking degree(MC)and high crosslinking degree(HC),were injected into the subcutaneous tissue,muscle and joints of the mouse model,respectively,in order to investigate the in vivo degradation pattern of collagen by NIR-II live imaging.The results of NIR-II imaging indicated that all tested collagens could be fully degraded after 35 days in the subcutaneous tissue,muscle and joints of the mouse model.However,the average degradation rate of subcutaneous tissue(k=0.13)and muscle(k=0.23)was slower than that of the joints(shoulder:k=0.42,knee:k=0.55).Specifically,the degradation rate of HC(k=0.13)was slower than LC(k=0.30)in muscle,while HC showed the fastest degradation rate in the shoulder and knee joints.In summary,NIR-II imaging could precisely identify the in vivo degradation rate of collagen.Moreover,the degradation rate of collagen was more closely related to the implanted body parts rather than the crosslinking degree of collagen,which was slower in the subcutaneous tissue and muscle compared to the joints in the mouse model.
基金supported by the National Natural Science Foundation of China (Grant No.60573019)Guangdong Natural Science Foundation (No.05300198 and 05103541).
文摘This paper proposes a technique of image content authentication based on the Laplacian Pyramid to verify the authenticity of image content.First,the image is decomposed into Laplacian Pyramid before the transformation.Next,the smooth and detail properties of the original image are analyzed according to the Laplacian Pyramid,and the properties are classified and encoded to get the corresponding characteristic values.Then,the signature derived from the encrypted characteristic values is embedded in the original image as a watermark.After the reception,the characteristic values of the received image are compared with the watermark drawn out from the image.The algorithm automatically identifies whether the content is tampered by means of morphologic filtration.The information of tampered location is p resented at the same time.Experimental results show that the proposed authentication algorithm can effectively detect the event and location when the original image content is tampered.Moreover,it can tolerate some distortions produced by compression,filtration and noise degradation.
