Hepatocellular carcinoma(HCC)is one of the most common malignant tumors globally and is the most prevalent type of primary liver cancer,posing a heavy burden on global health.Surgical resection and liver transplantati...Hepatocellular carcinoma(HCC)is one of the most common malignant tumors globally and is the most prevalent type of primary liver cancer,posing a heavy burden on global health.Surgical resection and liver transplantation are the gold standard for the radical treatment of HCC.However,due to the heterogeneity and high invasiveness of HCC,the rates of local and distant recurrence are extremely high,with over 70%of patients experiencing recurrence within 5 years after treatment,significantly impacting the long-term quality of life.Therefore,researchers are exploring other treatment methods to reduce tumor recurrence and improve patient survival.To date,extensive research has concentrated on new alternative therapies,including radiotherapy(e.g.,selective internal radiotherapy),targeted drug therapy(e.g.,sorafenib and lenvatinib),and immunotherapy(e.g.,immune checkpoint inhibitors),which have played an integral role in the comprehensive treatment of HCC.This review mainly focuses on the cutting-edge advancements in these treatment methods for HCC and their potential role in reducing HCC recurrence.展开更多
Anaerobic fermentation is an efficient method to extract phosphorus from excess sludge,thereby facilitating its recovery and mitigating the phosphorus resource shortage.However,the prevalent metal-bound phosphorus spe...Anaerobic fermentation is an efficient method to extract phosphorus from excess sludge,thereby facilitating its recovery and mitigating the phosphorus resource shortage.However,the prevalent metal-bound phosphorus species within sludge was difficult to release into the fermentation liquor.To address this,this study evaluated the enhanced phosphorus release performance from sludge containing iron-phosphorus compounds(Fe-P)via co-fermenting it with agriculture wastes.Specifically,protein-rich feather(Feather Group)and polysaccharide-rich tea residue(Tea Group)was respectively dosed into batch-scale fermentation jar.Results showed that the Feather Group exhibited significantly higher levels of released soluble phosphorus(2.1 folds)and volatile fatty acids(41.4 folds)compared to the Control Group,with concentrations reaching up to 280 mg/L and 9366 mg chemical oxygen demand/L,respectively.The activities ofα-glucosidase,neutral protease and acetate kinase in the Feather group were increased by 11.1%,92.3%and 37.6%,respectively,compared with the Control group.Methanogen abundance decreased while hydrolytic acid-producing bacteria and iron-reducing bacteria increased significantly after supplying agricultural wastes.Metagenomic analysis demonstrated a significant increase in genes related to acetic acid synthesis.Mechanism elucidation suggested that increased iron-reducing bacteria abundance promoted Fe3+reduction into Fe2+,thus enhancing phosphorus release from Fe-P compounds.This work may provide valuable information for developing effective strategy to extract phosphorus resource from complex environmental wastes.展开更多
While a healthy lifestyle is known to reduce the risk of stroke,the extent to which blood pressure(BP)mediates this association remains unclear.The present study aimed to quantify the mediating role of BP in the assoc...While a healthy lifestyle is known to reduce the risk of stroke,the extent to which blood pressure(BP)mediates this association remains unclear.The present study aimed to quantify the mediating role of BP in the association between combined lifestyle factors and stroke incidence.Using data from 51929 participants free of major cardiovascular diseases or cancer at baseline,we employed structural equation modeling to assess the mediating effects of systolic(SBP)and diastolic(DBP)blood pressure.During the follow-up,2811 incident stroke cases were identified.A healthy lifestyle was significantly associated with a reduced risk of stroke,with SBP mediating 44.70%(β=-0.0014,95%confidence interval[CI]:-0.0016 to-0.0012)and DBP mediating 37.81%(β=-0.0012,95%CI:-0.0015 to-0.0009)of this association.The mediating effects were attenuated but remained significant for ischemic stroke(SBP:33.21%;DBP:27.24%).In conclusion,approximately two-fifths of the protective association between a healthy lifestyle and stroke may be mediated by BP.These findings suggest that BP control may serve as an important early indicator for evaluating the effectiveness of lifestyle interventions in reducing stroke risk.展开更多
Lithium(Li)metal is regarded as the ultimate anode for next-generation Li-ion batteries due to its highest specific capacity and lowest electrochemical potential.However,the Li metal anode has limitations,including vi...Lithium(Li)metal is regarded as the ultimate anode for next-generation Li-ion batteries due to its highest specific capacity and lowest electrochemical potential.However,the Li metal anode has limitations,including virtually infinite volume change,nonuniform Li deposition,and an unstable electrode-electrolyte interface,which lead to rapid capacity degradation and poor cycling stability,significantly hindering its practical application.To address these issues,intensive efforts have been devoted toward accommodating and guiding Li deposition as well as stabilizing the interface using various carbon materials,which have demonstrated excellent effectiveness,benefiting from their vast variety and excellent tunability of the structure-property relationship.This review is intended as a guide through the fundamental challenges of Li metal anodes to the corresponding solutions utilizing carbon materials.The specific functionalities and mechanisms of carbon materials for stabilizing Li metal anodes in these solutions are discussed in detail.Apart from the stabilization of the Li metal anode in liquid electrolytes,attention has also been paid to the review of anode-free Li metal batteries and solid-state batteries enabled by strategies based on carbon materials.Furthermore,we have reviewed the unresolved challenges and presented our outlook on the implementation of carbon materials for stabilizing Li metal anodes in practical applications.展开更多
OBJECTIVE： There are no convenient techniques to evaluate the degree of facial nerve injury during a course of acupuncture treatment for Bell＇s palsy. Our previous studies found that observing the electrical respons...OBJECTIVE： There are no convenient techniques to evaluate the degree of facial nerve injury during a course of acupuncture treatment for Bell＇s palsy. Our previous studies found that observing the electrical response of specific facial muscles provided reasonable correlation with the prognosis of electroacupuncture treatment. Hence, we used the new method to evaluate the degree of facial nerve injury in patients with Bell＇s palsy in comparison with the House-Brackmann scale. The relationship between therapeutic effects and prognosis was analyzed to explore an objective method for evaluating Bell＇s palsy. METHODS： The facial nerve function of 68 patients with Bell＇s palsy was assessed with both electrical response grading and the House-Brackmann scale before treatment. Then differences in evaluation results of the two methods were compared. All enrolled patients received electroacupuncture treatment with disperse-dense wave at 1/100 Hz for 4 weeks. After treatment, correlation analysis was conducted to find the relationship between electrical response and therapeutic effects or prognosis. RESULTS： Checking consistency between electrical response grading and House-Brackmann scale： Kappa value 0.028 （P = 0.578）. Correlation analysis： the two methods were correlated with the prognosis, and electrical response grading （rER = 0.789） was better than the House-Brackmann scale （rHB = 0.423）. CONCLUSION： Electrical response grading is superior to the House-Brackmann scale in efficacy and reliability, and can conveniently assess the degree of facial nerve injury. The House-Brackmann scale is suitable for the patients with mild facial nerve injury, but its evaluation quality for severe facial nerve injury is poor.展开更多
Porosity formation during solidification of aluminum-based alloys,due to hydrogen gas and alloy shrinkage,has been a major issue adversely affecting the performance of solidification products such as castings,welds or...Porosity formation during solidification of aluminum-based alloys,due to hydrogen gas and alloy shrinkage,has been a major issue adversely affecting the performance of solidification products such as castings,welds or additively manufactured components.A three-dimensional cellular automaton(CA)model has been developed,for the first time,to couple the predictions of hydrogen-induced gas porosity and shrinkage porosity during solidification microstructure evolution of a binary Al-Si alloy.The CA simulation results are validated under various cooling rates by porosity measurements in an experimental wedge die casting using X-ray micro computed tomography(XMCT)technique.This validated porosity moel provides a critical link in integrated computation materials engineering(ICME)design and manufacturing of solidification products.展开更多
Porosity is a major issue in solidification processing of metallic materials.In this work,wedge die casting experiments were designed to investigate the effect of cooling rate on microporosity in an aluminum alloy A35...Porosity is a major issue in solidification processing of metallic materials.In this work,wedge die casting experiments were designed to investigate the effect of cooling rate on microporosity in an aluminum alloy A356.Microstructure information including dendrites and porosity were measured and observed by optical microscopy and X-ray micro-computed tomography(XMCT).The effects of cooling rate on secondary dendrite arm spacing(SDAS)and porosity were discussed.The relationship between SDAS and cooling rate was established and validated using a mathematical model.Three-dimensional(3-D)porosity information,including porosity percentage,pore volume,and pore number,was determined by XMCT.With the cooling rate decreasing from a lower to a higher position of the wedge die,the observed pore number decreases,the porosity percentage increases,and the equivalent pore radius increases.Sphericity of the pores was discussed as an empirical criterion to distinguish the types of porosity.For different cooling rates,the larger the equivalent pore radius is,the lower the sphericity of the pores.This research suggests that XMCT is a useful tool to provide critical 3-D porosity information for integrated computational materials engineering(ICME)design and process optimization of solidification products.展开更多
Objective:The annual influenza epidemic is a heavy burden on the health care system,and has increasingly become a major public health problem in some areas,such as Hong Kong(China).Therefore,based on a variety of mach...Objective:The annual influenza epidemic is a heavy burden on the health care system,and has increasingly become a major public health problem in some areas,such as Hong Kong(China).Therefore,based on a variety of machine learning methods,and considering the seasonal influenza in Hong Kong,the study aims to establish a Combinatorial Judgment Classifier(CJC)model to classify the epidemic trend and improve the accuracy of influenza epidemic early warning.展开更多
The implementation of ultra-low emission(ULE)limits(SO_(2):35 mg/m3,NOx:50 mg/m3,PM:10 mg/m3)promoted the development of flue gas treatment technologies in China.Pollutant control technology development for Chinese co...The implementation of ultra-low emission(ULE)limits(SO_(2):35 mg/m3,NOx:50 mg/m3,PM:10 mg/m3)promoted the development of flue gas treatment technologies in China.Pollutant control technology development for Chinese coal-fired power plants was summarized and an analysis of the applicability and cost of pollutant control technologies was conducted.Detailed data were collected from 30 ultra-low emission coal-fired units across China.Based on a cost analysis model,the average unit power generation incremental costs were 0.0144and 0.0095 CNY/(kW·hr)for SO_(2)and NOx control technologies,respectively.The unit power generation incremental cost of twin spray tower technology was 7.2%higher than that of dual-loop spray tower technology.The effect of key parameters on operating cost was analyzed.The unit power generation incremental cost increased because of increments in the electricity price for SO_(2)control technology and the price of the reductant in NOx control technology.With high sulfur content or NOx concentration,the unit power generation incremental cost caused by pollutant control increased,whereas the unit pollutant abatement cost decreased.However,the annual operating hours or load increased,thereby leading to a decline in unit power generation incremental cost and unit pollutant abatement cost.展开更多
Objective To explore the molecular mechanism of type 2 diabetes in intrauterine growth restricted adult rats through determination of blood glucose and expression of gluconeogenic enzymes in liver.Methods Male intraut...Objective To explore the molecular mechanism of type 2 diabetes in intrauterine growth restricted adult rats through determination of blood glucose and expression of gluconeogenic enzymes in liver.Methods Male intrauterine growth restriction(IUGR) offspring induced by maternal protein-malnutrition and normal controls were studied.The body weights of offspring rats were weighted from birth to 12 weeks of age.Fasting plasma glucose and insulin levels were determined by glucose oxidase method and enzyme-linked immunosorbent assay(ELISA) respectively at 1 week,8 weeks,and 12 weeks.Peroxisome proliferator-activated receptor-γ coactivator-1α(PGC-1α),phosphoenolpyruvate carboxykinase(PEPCK),and glucose-6-phosphatase(G6Pase) mRNA and protein levels in liver were measured by real time RT-PCR and Western blot in newborn rats(Week 1) and adult rats(Week 12).Results Birth weights of IUGR rats were significantly lower than those of controls until 4 weeks later,when IUGR rats caught up to controls.Between 8 and 12 weeks,the growth of IUGR rats surpassed that of controls.No significant differences were observed in blood glucose and insulin levels at newborn rats between the two groups.However,by the end of 8 weeks IUGR rats developed hyperinsulinemia and high insulin resistance index.At the age of 12 weeks,IUGR rats had mild fasting hyperglycemia.In addition,hepatic PGC-1α mRNA and protein levels as well as hepatic mRNA levels of PEPCK and G6Pase at Week 1 and Week 12 in IUGR rats were all significantly higher than those of controls(P<0.05).Conclusions As a result of intrauterine malnutrition,the expression of gluconeogenic genes is exaggerated in offspring.This change stays through adulthood and may contribute to the pathogenesis of type 2 diabetes.展开更多
The geometry optimizations and the single point energy calculations of iron tetraphenylporphyrin chloride Fe（TPP）Cl and iron tetraphenylporphyrin chloride （Fe（TPP）Cl）, iron pentafluorophenylporphyrin chloride ...The geometry optimizations and the single point energy calculations of iron tetraphenylporphyrin chloride Fe（TPP）Cl and iron tetraphenylporphyrin chloride （Fe（TPP）Cl）, iron pentafluorophenylporphyrin chloride （Fe（TPPF20）Cl） were carried out by using the Density Functional Theory （DFT） UB3LYP with STO-3G^＊ and 6-31G^＊ basis sets, respectively. The electronic properties and the structures of high-lying molecular orbitals were analyzed in detail. The results show that partial spin is transferred from the Fe atom to the porphyrin ring and some electron with the spin opposite to the unpaired electron on the Fe atom is transferred from the porphyrin ring to the Fe atom. The π and σ-type bonding between the Fe atom and the porphyin ring cause the transfer. The fluorination enhances the electron transfer and the chemical stability of the complex. The high stability is important for the complex possessing high catalytic activity. The catalysis mechanism of oxygen molecule activation on the complex surface is also discussed based on the symmetry of the molecular orbitals.展开更多
OBJECTIVE To investigate apoptosis induced by Bax in hepatocellular carcinoma cells and to examine the results of 2 different routes for in vivo gene delivery. METHODS The anti-hepatocellular carcinoma activity of the...OBJECTIVE To investigate apoptosis induced by Bax in hepatocellular carcinoma cells and to examine the results of 2 different routes for in vivo gene delivery. METHODS The anti-hepatocellular carcinoma activity of the Bax gene transferred to the human hepatocellular carcinoma QGY7703 cell line was examined. In addition the Bax gene was transferred in vivo in mice via the caudal vein or hepatic artery to investigate the differences in target organ and non-target organ transfection. RESULTS 1）The Bax gene mediated by a binary adenoviral vector system induced apoptosis in the human hepatic carcinoma QFY7703 cell line. The cell apoptotic rate in the experimental group （Bax） was 50.2±6.9% but only 32.1 ± 9.7% in the Ad/CMV-p53 group, showing that the Bax-apoptotic rate was significantly higher than the control group. 2） LacZ expression was higher in the target organ （liver） when given through the hepatic artery than through the tail vein. In contrast, LacZ expression in the nontarget organs was higher if given through the tail vein compared to the hepatic artery. CONCLUSION Superselective hepatic artery delivery with Bax gene therapy is safe, specific, effective and has low toxicity. This study provided the basis for Bax-gene therapy via the hepatic artery in vivo.展开更多
基金Supported by the National Natural Science Foundation of China,No.82270634Third Affiliated Hospital of Naval Medical University,No.tf2024yzyy01.
文摘Hepatocellular carcinoma(HCC)is one of the most common malignant tumors globally and is the most prevalent type of primary liver cancer,posing a heavy burden on global health.Surgical resection and liver transplantation are the gold standard for the radical treatment of HCC.However,due to the heterogeneity and high invasiveness of HCC,the rates of local and distant recurrence are extremely high,with over 70%of patients experiencing recurrence within 5 years after treatment,significantly impacting the long-term quality of life.Therefore,researchers are exploring other treatment methods to reduce tumor recurrence and improve patient survival.To date,extensive research has concentrated on new alternative therapies,including radiotherapy(e.g.,selective internal radiotherapy),targeted drug therapy(e.g.,sorafenib and lenvatinib),and immunotherapy(e.g.,immune checkpoint inhibitors),which have played an integral role in the comprehensive treatment of HCC.This review mainly focuses on the cutting-edge advancements in these treatment methods for HCC and their potential role in reducing HCC recurrence.
基金supported by the“Pioneer”and“Leading Goose”R&D Program of Zhejiang(No.2023C03149).
文摘Anaerobic fermentation is an efficient method to extract phosphorus from excess sludge,thereby facilitating its recovery and mitigating the phosphorus resource shortage.However,the prevalent metal-bound phosphorus species within sludge was difficult to release into the fermentation liquor.To address this,this study evaluated the enhanced phosphorus release performance from sludge containing iron-phosphorus compounds(Fe-P)via co-fermenting it with agriculture wastes.Specifically,protein-rich feather(Feather Group)and polysaccharide-rich tea residue(Tea Group)was respectively dosed into batch-scale fermentation jar.Results showed that the Feather Group exhibited significantly higher levels of released soluble phosphorus(2.1 folds)and volatile fatty acids(41.4 folds)compared to the Control Group,with concentrations reaching up to 280 mg/L and 9366 mg chemical oxygen demand/L,respectively.The activities ofα-glucosidase,neutral protease and acetate kinase in the Feather group were increased by 11.1%,92.3%and 37.6%,respectively,compared with the Control group.Methanogen abundance decreased while hydrolytic acid-producing bacteria and iron-reducing bacteria increased significantly after supplying agricultural wastes.Metagenomic analysis demonstrated a significant increase in genes related to acetic acid synthesis.Mechanism elucidation suggested that increased iron-reducing bacteria abundance promoted Fe3+reduction into Fe2+,thus enhancing phosphorus release from Fe-P compounds.This work may provide valuable information for developing effective strategy to extract phosphorus resource from complex environmental wastes.
基金supported by grants from the National Natural Science Foundation of China(Grant Nos.82192900,82192901,82192904,81390540,and 91846303 to L.L.)the National Key Research and Development Program of China(Grant No.2016YFC0900500 to Y.G.)the Kadoorie Charitable Foundation in Hong Kong,and the Wellcome Trust in the UK(Grant/Award Nos.088158/Z/09/Z,104085/Z/14/Z,and 202922/Z/16/Z to Z.C.).
文摘While a healthy lifestyle is known to reduce the risk of stroke,the extent to which blood pressure(BP)mediates this association remains unclear.The present study aimed to quantify the mediating role of BP in the association between combined lifestyle factors and stroke incidence.Using data from 51929 participants free of major cardiovascular diseases or cancer at baseline,we employed structural equation modeling to assess the mediating effects of systolic(SBP)and diastolic(DBP)blood pressure.During the follow-up,2811 incident stroke cases were identified.A healthy lifestyle was significantly associated with a reduced risk of stroke,with SBP mediating 44.70%(β=-0.0014,95%confidence interval[CI]:-0.0016 to-0.0012)and DBP mediating 37.81%(β=-0.0012,95%CI:-0.0015 to-0.0009)of this association.The mediating effects were attenuated but remained significant for ischemic stroke(SBP:33.21%;DBP:27.24%).In conclusion,approximately two-fifths of the protective association between a healthy lifestyle and stroke may be mediated by BP.These findings suggest that BP control may serve as an important early indicator for evaluating the effectiveness of lifestyle interventions in reducing stroke risk.
基金support from the Federal Ministry of Education and Research(BMBF)under project“KaSiLi”(03XP0254D)in the competence cluster“ExcellBattMat.”。
文摘Lithium(Li)metal is regarded as the ultimate anode for next-generation Li-ion batteries due to its highest specific capacity and lowest electrochemical potential.However,the Li metal anode has limitations,including virtually infinite volume change,nonuniform Li deposition,and an unstable electrode-electrolyte interface,which lead to rapid capacity degradation and poor cycling stability,significantly hindering its practical application.To address these issues,intensive efforts have been devoted toward accommodating and guiding Li deposition as well as stabilizing the interface using various carbon materials,which have demonstrated excellent effectiveness,benefiting from their vast variety and excellent tunability of the structure-property relationship.This review is intended as a guide through the fundamental challenges of Li metal anodes to the corresponding solutions utilizing carbon materials.The specific functionalities and mechanisms of carbon materials for stabilizing Li metal anodes in these solutions are discussed in detail.Apart from the stabilization of the Li metal anode in liquid electrolytes,attention has also been paid to the review of anode-free Li metal batteries and solid-state batteries enabled by strategies based on carbon materials.Furthermore,we have reviewed the unresolved challenges and presented our outlook on the implementation of carbon materials for stabilizing Li metal anodes in practical applications.
文摘OBJECTIVE： There are no convenient techniques to evaluate the degree of facial nerve injury during a course of acupuncture treatment for Bell＇s palsy. Our previous studies found that observing the electrical response of specific facial muscles provided reasonable correlation with the prognosis of electroacupuncture treatment. Hence, we used the new method to evaluate the degree of facial nerve injury in patients with Bell＇s palsy in comparison with the House-Brackmann scale. The relationship between therapeutic effects and prognosis was analyzed to explore an objective method for evaluating Bell＇s palsy. METHODS： The facial nerve function of 68 patients with Bell＇s palsy was assessed with both electrical response grading and the House-Brackmann scale before treatment. Then differences in evaluation results of the two methods were compared. All enrolled patients received electroacupuncture treatment with disperse-dense wave at 1/100 Hz for 4 weeks. After treatment, correlation analysis was conducted to find the relationship between electrical response and therapeutic effects or prognosis. RESULTS： Checking consistency between electrical response grading and House-Brackmann scale： Kappa value 0.028 （P = 0.578）. Correlation analysis： the two methods were correlated with the prognosis, and electrical response grading （rER = 0.789） was better than the House-Brackmann scale （rHB = 0.423）. CONCLUSION： Electrical response grading is superior to the House-Brackmann scale in efficacy and reliability, and can conveniently assess the degree of facial nerve injury. The House-Brackmann scale is suitable for the patients with mild facial nerve injury, but its evaluation quality for severe facial nerve injury is poor.
基金the National Science Foundation for supporting this work(Award CMMI-1432688)supported by Honda R&D Americas(Raymond,Ohio)。
文摘Porosity formation during solidification of aluminum-based alloys,due to hydrogen gas and alloy shrinkage,has been a major issue adversely affecting the performance of solidification products such as castings,welds or additively manufactured components.A three-dimensional cellular automaton(CA)model has been developed,for the first time,to couple the predictions of hydrogen-induced gas porosity and shrinkage porosity during solidification microstructure evolution of a binary Al-Si alloy.The CA simulation results are validated under various cooling rates by porosity measurements in an experimental wedge die casting using X-ray micro computed tomography(XMCT)technique.This validated porosity moel provides a critical link in integrated computation materials engineering(ICME)design and manufacturing of solidification products.
文摘Porosity is a major issue in solidification processing of metallic materials.In this work,wedge die casting experiments were designed to investigate the effect of cooling rate on microporosity in an aluminum alloy A356.Microstructure information including dendrites and porosity were measured and observed by optical microscopy and X-ray micro-computed tomography(XMCT).The effects of cooling rate on secondary dendrite arm spacing(SDAS)and porosity were discussed.The relationship between SDAS and cooling rate was established and validated using a mathematical model.Three-dimensional(3-D)porosity information,including porosity percentage,pore volume,and pore number,was determined by XMCT.With the cooling rate decreasing from a lower to a higher position of the wedge die,the observed pore number decreases,the porosity percentage increases,and the equivalent pore radius increases.Sphericity of the pores was discussed as an empirical criterion to distinguish the types of porosity.For different cooling rates,the larger the equivalent pore radius is,the lower the sphericity of the pores.This research suggests that XMCT is a useful tool to provide critical 3-D porosity information for integrated computational materials engineering(ICME)design and process optimization of solidification products.
基金This project was supported by grants from the Ministry of Education Humanities and Social Sciences Research Fund Project。
文摘Objective:The annual influenza epidemic is a heavy burden on the health care system,and has increasingly become a major public health problem in some areas,such as Hong Kong(China).Therefore,based on a variety of machine learning methods,and considering the seasonal influenza in Hong Kong,the study aims to establish a Combinatorial Judgment Classifier(CJC)model to classify the epidemic trend and improve the accuracy of influenza epidemic early warning.
基金supported by the National Key Research and Development Program of China(No.2019YFC0214803)Zhejiang Provincial Natural Science Foundation of China(No.LQ21D050002).
文摘The implementation of ultra-low emission(ULE)limits(SO_(2):35 mg/m3,NOx:50 mg/m3,PM:10 mg/m3)promoted the development of flue gas treatment technologies in China.Pollutant control technology development for Chinese coal-fired power plants was summarized and an analysis of the applicability and cost of pollutant control technologies was conducted.Detailed data were collected from 30 ultra-low emission coal-fired units across China.Based on a cost analysis model,the average unit power generation incremental costs were 0.0144and 0.0095 CNY/(kW·hr)for SO_(2)and NOx control technologies,respectively.The unit power generation incremental cost of twin spray tower technology was 7.2%higher than that of dual-loop spray tower technology.The effect of key parameters on operating cost was analyzed.The unit power generation incremental cost increased because of increments in the electricity price for SO_(2)control technology and the price of the reductant in NOx control technology.With high sulfur content or NOx concentration,the unit power generation incremental cost caused by pollutant control increased,whereas the unit pollutant abatement cost decreased.However,the annual operating hours or load increased,thereby leading to a decline in unit power generation incremental cost and unit pollutant abatement cost.
基金Supported by the National Natural Science Foundation of China(30672237)
文摘Objective To explore the molecular mechanism of type 2 diabetes in intrauterine growth restricted adult rats through determination of blood glucose and expression of gluconeogenic enzymes in liver.Methods Male intrauterine growth restriction(IUGR) offspring induced by maternal protein-malnutrition and normal controls were studied.The body weights of offspring rats were weighted from birth to 12 weeks of age.Fasting plasma glucose and insulin levels were determined by glucose oxidase method and enzyme-linked immunosorbent assay(ELISA) respectively at 1 week,8 weeks,and 12 weeks.Peroxisome proliferator-activated receptor-γ coactivator-1α(PGC-1α),phosphoenolpyruvate carboxykinase(PEPCK),and glucose-6-phosphatase(G6Pase) mRNA and protein levels in liver were measured by real time RT-PCR and Western blot in newborn rats(Week 1) and adult rats(Week 12).Results Birth weights of IUGR rats were significantly lower than those of controls until 4 weeks later,when IUGR rats caught up to controls.Between 8 and 12 weeks,the growth of IUGR rats surpassed that of controls.No significant differences were observed in blood glucose and insulin levels at newborn rats between the two groups.However,by the end of 8 weeks IUGR rats developed hyperinsulinemia and high insulin resistance index.At the age of 12 weeks,IUGR rats had mild fasting hyperglycemia.In addition,hepatic PGC-1α mRNA and protein levels as well as hepatic mRNA levels of PEPCK and G6Pase at Week 1 and Week 12 in IUGR rats were all significantly higher than those of controls(P<0.05).Conclusions As a result of intrauterine malnutrition,the expression of gluconeogenic genes is exaggerated in offspring.This change stays through adulthood and may contribute to the pathogenesis of type 2 diabetes.
基金ACKN0WLEDGMENT This work was supported by the National Natural Science Foundation of China （No.20443002） and the Science Foundations of Henan Province for 0utstanding Young Scientists （No.0612002600）
文摘The geometry optimizations and the single point energy calculations of iron tetraphenylporphyrin chloride Fe（TPP）Cl and iron tetraphenylporphyrin chloride （Fe（TPP）Cl）, iron pentafluorophenylporphyrin chloride （Fe（TPPF20）Cl） were carried out by using the Density Functional Theory （DFT） UB3LYP with STO-3G^＊ and 6-31G^＊ basis sets, respectively. The electronic properties and the structures of high-lying molecular orbitals were analyzed in detail. The results show that partial spin is transferred from the Fe atom to the porphyrin ring and some electron with the spin opposite to the unpaired electron on the Fe atom is transferred from the porphyrin ring to the Fe atom. The π and σ-type bonding between the Fe atom and the porphyin ring cause the transfer. The fluorination enhances the electron transfer and the chemical stability of the complex. The high stability is important for the complex possessing high catalytic activity. The catalysis mechanism of oxygen molecule activation on the complex surface is also discussed based on the symmetry of the molecular orbitals.
文摘OBJECTIVE To investigate apoptosis induced by Bax in hepatocellular carcinoma cells and to examine the results of 2 different routes for in vivo gene delivery. METHODS The anti-hepatocellular carcinoma activity of the Bax gene transferred to the human hepatocellular carcinoma QGY7703 cell line was examined. In addition the Bax gene was transferred in vivo in mice via the caudal vein or hepatic artery to investigate the differences in target organ and non-target organ transfection. RESULTS 1）The Bax gene mediated by a binary adenoviral vector system induced apoptosis in the human hepatic carcinoma QFY7703 cell line. The cell apoptotic rate in the experimental group （Bax） was 50.2±6.9% but only 32.1 ± 9.7% in the Ad/CMV-p53 group, showing that the Bax-apoptotic rate was significantly higher than the control group. 2） LacZ expression was higher in the target organ （liver） when given through the hepatic artery than through the tail vein. In contrast, LacZ expression in the nontarget organs was higher if given through the tail vein compared to the hepatic artery. CONCLUSION Superselective hepatic artery delivery with Bax gene therapy is safe, specific, effective and has low toxicity. This study provided the basis for Bax-gene therapy via the hepatic artery in vivo.
