BACKGROUND:This study aimed to explore the importance of routine coagulation tests for the early detection of sepsis and to quickly identify patients at a high risk of mortality.METHODS:This retrospective single-cente...BACKGROUND:This study aimed to explore the importance of routine coagulation tests for the early detection of sepsis and to quickly identify patients at a high risk of mortality.METHODS:This retrospective single-center study collected data from patients meeting the clinical criteria for systemic inflammatory response syndrome(SIRS) with a confirmed infection source.Patients with coagulation disorders or on medications affecting coagulation were excluded.Patients were divided into sepsis and non-sepsis groups based on a Sequential Organ Failure Assessment(SOFA) score of ≥2.Univariate and multivariate logistic regression identified indicators from routine coagulation tests that predict sepsis.Prognostic roles of coagulation indicators were analyzed within the sepsis group.RESULTS:A total of 512 patients were included,with 396 in the sepsis group and 116 in the non-sepsis group.The predictive factors in the sepsis prediction model encompass fibrin degradation products(FDP),D-dimer,lactate,procalcitonin(PCT) levels and the utilization of mechanical ventilation.Early elevation of FDP and D-dimer levels predicted sepsis onset.The model exhibited an area under the curve(AUC) of 0.943(95% CI:0.923–0.963).In the sepsis group,Cox regression analysis revealed an association between prothrombin time(PT) and in-hospital mortality.CONCLUSION:Abnormal high FDP and D-dimer levels in the early stages of sepsis provide a supplementary method for predicting sepsis.As the disease progresses,prolonged PT in the early stages of sepsis suggests a poor prognosis.展开更多
Temperature-dependent sex determination（TSD） is a type of environmental sex determination in which the sex of the embryos depends on the ambient temperature; however,the molecular mechanisms governing this process r...Temperature-dependent sex determination（TSD） is a type of environmental sex determination in which the sex of the embryos depends on the ambient temperature; however,the molecular mechanisms governing this process remain unknown.Aromatase,encoded by the cyp19a1 gene,which converts androgens into estrogens in animals,was considered to be the key gene for TSD.In this study,the 5＇-flanking region of the cyp19a1 gene in Reeves＇ turtle（Mauremys reevesii） was cloned,and the promoter region was identified using the luciferase reporter assay.Then the eggs of Reeves＇ turtle were incubated at different temperatures（26°C： male-biased temperature; 29°C： non-sex-biased temperature and 32°C： female-biased temperature）.During the thermosensitive period,the adrenal kidney gonad complexes（AKG） were sampled.DNA methylation analysis of the AKG samples showed that the promoter region of the cyp19a1 gene was significantly de-methylated in the female-biased temperature regime（P＆lt;0.01）.Quantitative analysis of the cyp19a1 gene and estrogen by q PCR and Elisa assay showed that the expression level of the cyp19a1 gene and estrogen content were both upregulated significantly at the female-biased temperature（P＆lt;0.01）.These results indicated that the de-methylation response of the cyp19a1 gene to incubation temperature,especially at the female-biased temperature,could lead to temperature-specific expression of aromatase and increased estrogen levels,which may further determine gonadal development in Reeves＇ turtle.These findings provide insights into the genetic mechanisms underlying TSD.展开更多
Coenzyme Q10(CoQ10)is the most consumed dietary supplement and mainly biosynthesized by aerobic fermentation of Rhodobacter sphaeroides(R.sphaeroides).Oxygen supply was identified as a bottleneck for improving CoQ10 y...Coenzyme Q10(CoQ10)is the most consumed dietary supplement and mainly biosynthesized by aerobic fermentation of Rhodobacter sphaeroides(R.sphaeroides).Oxygen supply was identified as a bottleneck for improving CoQ10 yield in R.sphaeroides.In this study,a precise regulation strategy based on dielectric spectroscopy(DS)was applied to further improve CoQ10 biosynthesis by R.sphaeroide.First,a quantitative response model among viable cells,cell morphology,and oxygen uptake rate(OUR)was established.DS could be used to detect viable R.sphaeroides cells,and the relationship among cell morphology,CoQ10 biosynthesis,and OUR was found to be significant.Based on this model,the online specific oxygen consumption rate(QO2)control strategy was successfully applied to the CoQ10 fermentation process.QO2 controlled at 0.07±0.01×10−7mmol/cell/h was most favorable for CoQ10 biosynthesis,resulting in a 28.3%increase in CoQ10 production.Based on the multi-parameters analysis and online QO2 control,a precise online nutrient feeding strategy was established using conductivity detected by DS.CoQ10 production was improved by 35%,reaching 3384 mg/L in 50 L bioreactors.This online control strategy would be effectively applied for improving industrial CoQ10 production,and the precise fermentation control strategy could also be applied to other fermentation process.展开更多
背景:难复性股骨转子间骨折往往无法通过牵引床牵引得到良好的复位,需要切开或者应用其他微创复位技术进行辅助复位。目的:探讨侧卧位牵引复位股骨近端防旋髓内钉固定对难复性股骨转子间骨折的复位效果。方法:回顾性分析2013年5月至2015...背景:难复性股骨转子间骨折往往无法通过牵引床牵引得到良好的复位,需要切开或者应用其他微创复位技术进行辅助复位。目的:探讨侧卧位牵引复位股骨近端防旋髓内钉固定对难复性股骨转子间骨折的复位效果。方法:回顾性分析2013年5月至2015年8月收治的84例难复性股骨转子间骨折患者的资料,均为新鲜骨折,骨折按AO分型:A2型26例,A3型58例。根据骨折复位方法的不同,分为侧卧位手法牵引复位组(A组)和平卧位牵引床牵引复位组(B组),每组42例。分析比较2组患者骨折复位质量、闭合复位失败率、术中失血量、手术时间、透视次数、骨折愈合时间及术后1年Harris评分。结果与结论:(1)A组患者复位效果优于B组,手术时间减少了13 min,术中出血量减少了50.65 m L,透视次数减少了18.59次,术后1年Harris评分增加了10.58分,2组比较差异有显著性意义(P<0.05);(2)提示对于难复性股骨转子间骨折,侧卧位手法牵引复位股骨近端防旋髓内钉固定可以缩短复位操作时间,减少术中血液丢失,提高复位质量,较牵引床复位更有优势。展开更多
基金supported by the Science and Technology Commission of Shanghai Municipality (grant no.22Y11900300)China Primary Health Care Foundation (grant no.MTP2022A0132)Project of Shanghai Administration of Traditional Chinese Medicine (ZXXT-202213)to EQM and LM。
文摘BACKGROUND:This study aimed to explore the importance of routine coagulation tests for the early detection of sepsis and to quickly identify patients at a high risk of mortality.METHODS:This retrospective single-center study collected data from patients meeting the clinical criteria for systemic inflammatory response syndrome(SIRS) with a confirmed infection source.Patients with coagulation disorders or on medications affecting coagulation were excluded.Patients were divided into sepsis and non-sepsis groups based on a Sequential Organ Failure Assessment(SOFA) score of ≥2.Univariate and multivariate logistic regression identified indicators from routine coagulation tests that predict sepsis.Prognostic roles of coagulation indicators were analyzed within the sepsis group.RESULTS:A total of 512 patients were included,with 396 in the sepsis group and 116 in the non-sepsis group.The predictive factors in the sepsis prediction model encompass fibrin degradation products(FDP),D-dimer,lactate,procalcitonin(PCT) levels and the utilization of mechanical ventilation.Early elevation of FDP and D-dimer levels predicted sepsis onset.The model exhibited an area under the curve(AUC) of 0.943(95% CI:0.923–0.963).In the sepsis group,Cox regression analysis revealed an association between prothrombin time(PT) and in-hospital mortality.CONCLUSION:Abnormal high FDP and D-dimer levels in the early stages of sepsis provide a supplementary method for predicting sepsis.As the disease progresses,prolonged PT in the early stages of sepsis suggests a poor prognosis.
基金supported financially by the National Natural Science Foundation of China(Nos.31401053 and 31471966)Guangdong Provincial Natural Science Foundation of China(No.2015A030313903)+1 种基金GDAS Special Project of Science and Technology Development(2017GDASCX-0107)the Funds for Environment Construction and Capacity Building of GDAS’Research Platform(2016GDASPT-0107)
文摘Temperature-dependent sex determination（TSD） is a type of environmental sex determination in which the sex of the embryos depends on the ambient temperature; however,the molecular mechanisms governing this process remain unknown.Aromatase,encoded by the cyp19a1 gene,which converts androgens into estrogens in animals,was considered to be the key gene for TSD.In this study,the 5＇-flanking region of the cyp19a1 gene in Reeves＇ turtle（Mauremys reevesii） was cloned,and the promoter region was identified using the luciferase reporter assay.Then the eggs of Reeves＇ turtle were incubated at different temperatures（26°C： male-biased temperature; 29°C： non-sex-biased temperature and 32°C： female-biased temperature）.During the thermosensitive period,the adrenal kidney gonad complexes（AKG） were sampled.DNA methylation analysis of the AKG samples showed that the promoter region of the cyp19a1 gene was significantly de-methylated in the female-biased temperature regime（P＆lt;0.01）.Quantitative analysis of the cyp19a1 gene and estrogen by q PCR and Elisa assay showed that the expression level of the cyp19a1 gene and estrogen content were both upregulated significantly at the female-biased temperature（P＆lt;0.01）.These results indicated that the de-methylation response of the cyp19a1 gene to incubation temperature,especially at the female-biased temperature,could lead to temperature-specific expression of aromatase and increased estrogen levels,which may further determine gonadal development in Reeves＇ turtle.These findings provide insights into the genetic mechanisms underlying TSD.
基金supported by the financial support of research projects from the National Key R&D Program of China(No.2021YFC2101000)Shanghai Scientific and Technological Innovation Action Plans-Scientific Instrument Development,China(grant No.21142201300,22142201000)+1 种基金National Natural Science Foundation of China(No.32071471 and No.22078295)Postgraduate Research&Practice Innovation Program of Jiangsu Province(KYCX21_2866).
文摘Coenzyme Q10(CoQ10)is the most consumed dietary supplement and mainly biosynthesized by aerobic fermentation of Rhodobacter sphaeroides(R.sphaeroides).Oxygen supply was identified as a bottleneck for improving CoQ10 yield in R.sphaeroides.In this study,a precise regulation strategy based on dielectric spectroscopy(DS)was applied to further improve CoQ10 biosynthesis by R.sphaeroide.First,a quantitative response model among viable cells,cell morphology,and oxygen uptake rate(OUR)was established.DS could be used to detect viable R.sphaeroides cells,and the relationship among cell morphology,CoQ10 biosynthesis,and OUR was found to be significant.Based on this model,the online specific oxygen consumption rate(QO2)control strategy was successfully applied to the CoQ10 fermentation process.QO2 controlled at 0.07±0.01×10−7mmol/cell/h was most favorable for CoQ10 biosynthesis,resulting in a 28.3%increase in CoQ10 production.Based on the multi-parameters analysis and online QO2 control,a precise online nutrient feeding strategy was established using conductivity detected by DS.CoQ10 production was improved by 35%,reaching 3384 mg/L in 50 L bioreactors.This online control strategy would be effectively applied for improving industrial CoQ10 production,and the precise fermentation control strategy could also be applied to other fermentation process.
文摘背景:难复性股骨转子间骨折往往无法通过牵引床牵引得到良好的复位,需要切开或者应用其他微创复位技术进行辅助复位。目的:探讨侧卧位牵引复位股骨近端防旋髓内钉固定对难复性股骨转子间骨折的复位效果。方法:回顾性分析2013年5月至2015年8月收治的84例难复性股骨转子间骨折患者的资料,均为新鲜骨折,骨折按AO分型:A2型26例,A3型58例。根据骨折复位方法的不同,分为侧卧位手法牵引复位组(A组)和平卧位牵引床牵引复位组(B组),每组42例。分析比较2组患者骨折复位质量、闭合复位失败率、术中失血量、手术时间、透视次数、骨折愈合时间及术后1年Harris评分。结果与结论:(1)A组患者复位效果优于B组,手术时间减少了13 min,术中出血量减少了50.65 m L,透视次数减少了18.59次,术后1年Harris评分增加了10.58分,2组比较差异有显著性意义(P<0.05);(2)提示对于难复性股骨转子间骨折,侧卧位手法牵引复位股骨近端防旋髓内钉固定可以缩短复位操作时间,减少术中血液丢失,提高复位质量,较牵引床复位更有优势。
