Objectives:Metastatic spread to the lung is one of the leading causes of fatal outcomes in thyroid cancer,but the underlying molecular mechanisms remain unclear.To investigate how exosomal microRNA-17-5p(miR-17-5p)pro...Objectives:Metastatic spread to the lung is one of the leading causes of fatal outcomes in thyroid cancer,but the underlying molecular mechanisms remain unclear.To investigate how exosomal microRNA-17-5p(miR-17-5p)promotes lung metastasis in thyroid cancer within the framework of the“seed and soil”hypothesis.Methods:Serum exosomes from thyroid cancer lung metastasis patients and controls were analyzed for miR-17,which was elevated in metastatic cases.miR-17 was transfected into embryonic lung fibroblasts(MRC-5),and their supernatants were cocultured with thyroid cancer cells(Cal62).Cell proliferation and migration were evaluated using colony formation,Ki67 staining,and Transwell assays.Interleukin-6(IL-6)/interleukin-8(IL-8)levels and nuclear factor kappa-B(NF-κB)/nuclear factor kappa-B repressing factor(NKRF)expression were analysed by enzyme-linked immunosorbent assays(ELISA)and western blot.In vivo models verified the metastatic-promoting effect of miR-17.Results:miR-17-5p was significantly enriched in serum exosomes of metastatic patients.In MRC-5 cells,it suppressed NKRF,NF-κB signaling,and increased secretion of IL-6 and IL-8,enhancing Cal62 proliferation and migration.Animal experiments confirmed its role in promoting tumor growth and lung metastasis.Conclusions:Exosomal miR-17-5p remodels the pulmonary microenvironment into a pro-inflammatory niche,facilitating thyroid cancer colonization and offering a potential therapeutic target.展开更多
目的基于CiteSpace和VOSviewer文献计量学方法,分析白芍研究现状并预测未来的研究热点和发展趋势。方法分别从中国知网数据库、万方数据库、Web of Science数据库核心集中收集白芍研究文献,导入NoteExpress中删除重复文献、不合格文献...目的基于CiteSpace和VOSviewer文献计量学方法,分析白芍研究现状并预测未来的研究热点和发展趋势。方法分别从中国知网数据库、万方数据库、Web of Science数据库核心集中收集白芍研究文献,导入NoteExpress中删除重复文献、不合格文献并统一关键词,再利用Excel、CiteSpace、VOSviewer软件分别对中英文文献的发文量、发文期刊、发文作者、发文机构、发文国家、文献被引频次、关键词等进行可视化分析。结果共检索到符合要求的文献4046篇,其中中文文献3334篇,英文文献712篇。白芍研究相关的年发文量整体呈上升趋势,中英文发文量最多的机构分别为北京中医药大学(76篇)和扬州大学(73篇),发文量最多的作者分别为魏伟(24篇)、Tao Jun(66篇)。开展白芍研究的国家主要以中国为主,刊载量最多的中英文期刊分别是《中草药》和Journal of Ethnopharmacology。被引频次前10的中英文文献主要为白芍药理作用及其机制研究方向,包括抗炎、抗氧化、抗抑郁、镇痛等方面。结论白芍研究热点集中在数据挖掘、组方规律以及白芍及其药效成分的药理作用和作用机制方面,尤其在抗氧化、抗炎、神经保护、抗抑郁、调节肠道菌群等方面的作用机制的深入研究可能是未来主要的研究趋势。展开更多
The ability to quickly and intuitively edit digital content has become increasingly important in our everyday life.However,existing edit propagation methods for editing digital images are typically based on optimizati...The ability to quickly and intuitively edit digital content has become increasingly important in our everyday life.However,existing edit propagation methods for editing digital images are typically based on optimization with high computational cost for large inputs.Moreover,existing edit propagation methods are generally inefficient and highly time-consuming.Accordingly,to improve edit efficiency,this paper proposes a novel edit propagation method using a bilateral grid,which can achieve instant propagation of sparse image edits.Firstly,given an input image with user interactions,we resample each of its pixels into a regularly sampled bilateral grid,which facilitates efficient mapping from an image to the bilateral space.As a result,all pixels with the same feature information(color,coordinates)are clustered to the same grid,which can achieve the goal of reducing both the amount of image data processing and the cost of calculation.We then reformulate the propagation as a function of the interpolation problem in bilateral space,which is solved very efficiently using radial basis functions.Experimental results show that our method improves the efficiency of color editing,making it faster than existing edit approaches,and results in excellent edited images with high quality.展开更多
Hot-dip Zn-Ti galvanizing can restrain the excess growth of the coating which has better corrosion resist- ances than hot-dip pure Zn. The change rules of Г2 intermetallic compound in hot-dip Zn-Ti galvanizing is inv...Hot-dip Zn-Ti galvanizing can restrain the excess growth of the coating which has better corrosion resist- ances than hot-dip pure Zn. The change rules of Г2 intermetallic compound in hot-dip Zn-Ti galvanizing is investiga ted by immersion time, titanium content and the slag test using scanning electron microscopy （SEM）, energy dis- persive spectroscopy （EDS） and X-ray diffraction （XRD）. And the mechanism of nucleation and growth of Г2 inter- metallic compound is analysed. The results show that, when adding 0. 05mass% titanium to the zinc bath, the Г2 particles form in the η layer, which nucleate by obtaining Ti atoms from the phase and Fe atoms from the dissol- ving ζ phase layer. Moreover, the more titanium is added into the bath, the more and bigger Гz particles appear. The Fz particles in the coatings grow up noticeably with prolonging the immersion time.展开更多
BACKGROUND Neoadjuvant chemotherapy(NAC)has become the standard care for advanced adenocarcinoma of esophagogastric junction(AEG),although a part of the patients cannot benefit from NAC.There are no models based on ba...BACKGROUND Neoadjuvant chemotherapy(NAC)has become the standard care for advanced adenocarcinoma of esophagogastric junction(AEG),although a part of the patients cannot benefit from NAC.There are no models based on baseline computed tomography(CT)to predict response of Siewert type II or III AEG to NAC with docetaxel,oxaliplatin and S-1(DOS).AIM To develop a CT-based nomogram to predict response of Siewert type II/III AEG to NAC with DOS.METHODS One hundred and twenty-eight consecutive patients with confirmed Siewert type II/III AEG underwent CT before and after three cycles of NAC with DOS,and were randomly and consecutively assigned to the training cohort(TC)(n=94)and the validation cohort(VC)(n=34).Therapeutic effect was assessed by disease-control rate and progressive disease according to the Response Evaluation Criteria in Solid Tumors(version 1.1)criteria.Possible prognostic factors associated with responses after DOS treatment including Siewert classification,gross tumor volume(GTV),and cT and cN stages were evaluated using pretherapeutic CT data in addition to sex and age.Univariate and multivariate analyses of CT and clinical features in the TC were performed to determine independent factors associated with response to DOS.A nomogram was established based on independent factors to predict the response.The predictive performance of the nomogram was evaluated by Concordance index(C-index),calibration and receiver operating characteristics curve in the TC and VC.RESULTS Univariate analysis showed that Siewert type(52/55 vs 29/39,P=0.005),pretherapeutic cT stage(57/62 vs 24/32,P=0.028),GTV(47.3±27.4 vs 73.2±54.3,P=0.040)were significantly associated with response to DOS in the TC.Multivariate analysis of the TC also showed that the pretherapeutic cT stage,GTV and Siewert type were independent predictive factors related to response to DOS(odds ratio=4.631,1.027 and 7.639,respectively;all P<0.05).The nomogram developed with these independent factors showed an excellent performance to predict response to DOS in the TC and VC(C-index:0.838 and 0.824),with area under the receiver operating characteristic curve of 0.838 and 0.824,respectively.The calibration curves showed that the practical and predicted response to DOS effectively coincided.CONCLUSION A novel nomogram developed with pretherapeutic cT stage,GTV and Siewert type predicted the response of Siewert type II/III AEG to NAC with DOS.展开更多
BACKGROUND Although papillary thyroid microcarcinoma(PTMC)is not considered a threatening tumor,in some cases,it can be aggressive.Metastatic thrombosis of papillary thyroid carcinoma,follicular thyroid carcinoma,H...BACKGROUND Although papillary thyroid microcarcinoma(PTMC)is not considered a threatening tumor,in some cases,it can be aggressive.Metastatic thrombosis of papillary thyroid carcinoma,follicular thyroid carcinoma,Hürthle cell carcinoma,poorly differentiated thyroid carcinoma and anaplastic thyroid carcinoma have been reported in the literature,but there have been no reports about PTMC.CASE SUMMARY A 45-year-old woman presented with a thyroid mass and thrombosis in a middle thyroid vein during a physical examination.She had no symptoms,and the physical examination showed no positive signs.Subsequent ultrasonographyguided fine-needle aspiration biopsy results indicated an atypical lesion of ambiguous significance,with some actively growing cells(TBSRTCⅢ)and the BRAFV600E mutation not present.This patient underwent left thyroidectomy,isthmus lobectomy,prophylactic central lymph node dissection and thromboembolectomy.Postoperative pathology showed papillary microcarcinoma of the left thyroid,and the thrombus in the middle thyroid vein was a tumor thrombus.CONCLUSION Middle thyroid vein tumor thrombus is an extremely rare condition in PTMC,but it does exist.Lobectomy and thromboembolectomy may be an option for patients with thrombi in the middle vein of the thyroid,and we strongly suggest close follow-up of these patients.展开更多
Objective:The aim of the study was to evaluate the inhibition of different chemotherapy drugs on γ-synuclein (SNCG) positive expression of breast cancer cell line MDA-MB231,and the effects on cell cycle and apoptosis...Objective:The aim of the study was to evaluate the inhibition of different chemotherapy drugs on γ-synuclein (SNCG) positive expression of breast cancer cell line MDA-MB231,and the effects on cell cycle and apoptosis,and to explore the related mechanism as well.Methods:We treated the breast cancer cell line MDA-MB231 for the inhibition of SNCG with chemotherapy drugs such as irinotecan,nedaplatin and 5-fluorouracil using RT-PCR and immunohistochemistry,and adopted flow cytometry to detect cell cycle distribution and apoptosis.Results:At the transcription and translation levels,the SNCG expression level in nedaplatin group and 5-fluorouracil group was lower than that of other groups and there was statistically significance (P < 0.01) compared with the control group,while there was not statistically significant between irinotecan group and the control group.After drugs action,cell cycle and distribution in each experiment group changed obviously,where the cells in G0G1 phase increased,especially the cells in the nedaplatin group and 5-fluorouracil group changed most significantly,as well as the obvious change in the cells of nedaplatin group and 5-fluorouracil group in the apoptosis period.Conclusion:There was a stronger inhibition of SNCG expression in nedaplatin and 5-fluorouracil groups,and can cause significant cell cycle and apoptosis changes.It may also be concluded that nedaplatin and 5-fluorouracil could make effects by the mechanisms of inhibiting cancer cell proliferation and inducing cell apoptosis.展开更多
The Brain-Computer Interfaces(BCIs)had been proposed and used in therapeutics for decades.However,the need of time-consuming calibration phase and the lack of robustness,which are caused by little-labeled data,are res...The Brain-Computer Interfaces(BCIs)had been proposed and used in therapeutics for decades.However,the need of time-consuming calibration phase and the lack of robustness,which are caused by little-labeled data,are restricting the advance and application of BCI,especially for the BCI based on motor imagery(MI).In this paper,we reviewed the recent development in the machine learning algorithm used in the MI-based BCI,which may provide potential solutions for addressing the issue.We classified these algorithms into two categories,namely,and enhancing the representation and expanding the training set.Specifically,these methods of enhancing the representation of features collected from few EEG trials are based on extracting features of multiple bands,regularization,and so on.The methods of expanding the training dataset include approaches of transfer learning(session to session transfer,subject to subject transfer)and generating artificial EEG data.The result of these techniques showed the resolution of the challenges to some extent.As a developing research area,the study of BCI algorithms in little-labeled data is increasingly requiring the advancement of human brain physiological structure research and more transfer learning algorithms research.展开更多
We synthesize animations from a single image by transferring fluid motion of a video example globally.Given a target image of a fluid scene,an alpha matte is required to extract the fluid region.Our method needs to ad...We synthesize animations from a single image by transferring fluid motion of a video example globally.Given a target image of a fluid scene,an alpha matte is required to extract the fluid region.Our method needs to adjust a user-specified video example for producing the fluid motion suitable for the extracted fluid region.Employing the fluid video database,the flow field of the target image is obtained by warping the optical flow of a video frame that has a visually similar scene to the target image according to their scene correspondences,which assigns fluid orientation and speed automatically.Results show that our method is successful in preserving large fluid features in the synthesized animations.In comparison to existing approaches,it is both possible and useful to utilize our method to create flow animations with higher quality.展开更多
基金supported by grants from the National Natural Science Foundation of China(Nos.82173125,81974374)General Support Projects of the Health Commission of Binhai New Area(2023BWKY024)Tianjin Key Medical Discipline Construction Project(TJYXZDXK044A).
文摘Objectives:Metastatic spread to the lung is one of the leading causes of fatal outcomes in thyroid cancer,but the underlying molecular mechanisms remain unclear.To investigate how exosomal microRNA-17-5p(miR-17-5p)promotes lung metastasis in thyroid cancer within the framework of the“seed and soil”hypothesis.Methods:Serum exosomes from thyroid cancer lung metastasis patients and controls were analyzed for miR-17,which was elevated in metastatic cases.miR-17 was transfected into embryonic lung fibroblasts(MRC-5),and their supernatants were cocultured with thyroid cancer cells(Cal62).Cell proliferation and migration were evaluated using colony formation,Ki67 staining,and Transwell assays.Interleukin-6(IL-6)/interleukin-8(IL-8)levels and nuclear factor kappa-B(NF-κB)/nuclear factor kappa-B repressing factor(NKRF)expression were analysed by enzyme-linked immunosorbent assays(ELISA)and western blot.In vivo models verified the metastatic-promoting effect of miR-17.Results:miR-17-5p was significantly enriched in serum exosomes of metastatic patients.In MRC-5 cells,it suppressed NKRF,NF-κB signaling,and increased secretion of IL-6 and IL-8,enhancing Cal62 proliferation and migration.Animal experiments confirmed its role in promoting tumor growth and lung metastasis.Conclusions:Exosomal miR-17-5p remodels the pulmonary microenvironment into a pro-inflammatory niche,facilitating thyroid cancer colonization and offering a potential therapeutic target.
文摘目的基于CiteSpace和VOSviewer文献计量学方法,分析白芍研究现状并预测未来的研究热点和发展趋势。方法分别从中国知网数据库、万方数据库、Web of Science数据库核心集中收集白芍研究文献,导入NoteExpress中删除重复文献、不合格文献并统一关键词,再利用Excel、CiteSpace、VOSviewer软件分别对中英文文献的发文量、发文期刊、发文作者、发文机构、发文国家、文献被引频次、关键词等进行可视化分析。结果共检索到符合要求的文献4046篇,其中中文文献3334篇,英文文献712篇。白芍研究相关的年发文量整体呈上升趋势,中英文发文量最多的机构分别为北京中医药大学(76篇)和扬州大学(73篇),发文量最多的作者分别为魏伟(24篇)、Tao Jun(66篇)。开展白芍研究的国家主要以中国为主,刊载量最多的中英文期刊分别是《中草药》和Journal of Ethnopharmacology。被引频次前10的中英文文献主要为白芍药理作用及其机制研究方向,包括抗炎、抗氧化、抗抑郁、镇痛等方面。结论白芍研究热点集中在数据挖掘、组方规律以及白芍及其药效成分的药理作用和作用机制方面,尤其在抗氧化、抗炎、神经保护、抗抑郁、调节肠道菌群等方面的作用机制的深入研究可能是未来主要的研究趋势。
基金supported by National Natural Science Foundation of China(No.U1836208,No.61402053 and No.61202439)Natural Science Foundation of Hunan Province of China(No.2019JJ50666 and No.2019JJ50655)partly supported by Open Fund of Hunan Key Laboratory of Smart Roadway and Cooperative Vehicle-Infrastructure Systems(Changsha University of Science&Technology)(No.KFJ180701).
文摘The ability to quickly and intuitively edit digital content has become increasingly important in our everyday life.However,existing edit propagation methods for editing digital images are typically based on optimization with high computational cost for large inputs.Moreover,existing edit propagation methods are generally inefficient and highly time-consuming.Accordingly,to improve edit efficiency,this paper proposes a novel edit propagation method using a bilateral grid,which can achieve instant propagation of sparse image edits.Firstly,given an input image with user interactions,we resample each of its pixels into a regularly sampled bilateral grid,which facilitates efficient mapping from an image to the bilateral space.As a result,all pixels with the same feature information(color,coordinates)are clustered to the same grid,which can achieve the goal of reducing both the amount of image data processing and the cost of calculation.We then reformulate the propagation as a function of the interpolation problem in bilateral space,which is solved very efficiently using radial basis functions.Experimental results show that our method improves the efficiency of color editing,making it faster than existing edit approaches,and results in excellent edited images with high quality.
基金Sponsored by Science and Technology Planning Project of Guangdong Province of China(2011B010300017)
文摘Hot-dip Zn-Ti galvanizing can restrain the excess growth of the coating which has better corrosion resist- ances than hot-dip pure Zn. The change rules of Г2 intermetallic compound in hot-dip Zn-Ti galvanizing is investiga ted by immersion time, titanium content and the slag test using scanning electron microscopy （SEM）, energy dis- persive spectroscopy （EDS） and X-ray diffraction （XRD）. And the mechanism of nucleation and growth of Г2 inter- metallic compound is analysed. The results show that, when adding 0. 05mass% titanium to the zinc bath, the Г2 particles form in the η layer, which nucleate by obtaining Ti atoms from the phase and Fe atoms from the dissol- ving ζ phase layer. Moreover, the more titanium is added into the bath, the more and bigger Гz particles appear. The Fz particles in the coatings grow up noticeably with prolonging the immersion time.
文摘BACKGROUND Neoadjuvant chemotherapy(NAC)has become the standard care for advanced adenocarcinoma of esophagogastric junction(AEG),although a part of the patients cannot benefit from NAC.There are no models based on baseline computed tomography(CT)to predict response of Siewert type II or III AEG to NAC with docetaxel,oxaliplatin and S-1(DOS).AIM To develop a CT-based nomogram to predict response of Siewert type II/III AEG to NAC with DOS.METHODS One hundred and twenty-eight consecutive patients with confirmed Siewert type II/III AEG underwent CT before and after three cycles of NAC with DOS,and were randomly and consecutively assigned to the training cohort(TC)(n=94)and the validation cohort(VC)(n=34).Therapeutic effect was assessed by disease-control rate and progressive disease according to the Response Evaluation Criteria in Solid Tumors(version 1.1)criteria.Possible prognostic factors associated with responses after DOS treatment including Siewert classification,gross tumor volume(GTV),and cT and cN stages were evaluated using pretherapeutic CT data in addition to sex and age.Univariate and multivariate analyses of CT and clinical features in the TC were performed to determine independent factors associated with response to DOS.A nomogram was established based on independent factors to predict the response.The predictive performance of the nomogram was evaluated by Concordance index(C-index),calibration and receiver operating characteristics curve in the TC and VC.RESULTS Univariate analysis showed that Siewert type(52/55 vs 29/39,P=0.005),pretherapeutic cT stage(57/62 vs 24/32,P=0.028),GTV(47.3±27.4 vs 73.2±54.3,P=0.040)were significantly associated with response to DOS in the TC.Multivariate analysis of the TC also showed that the pretherapeutic cT stage,GTV and Siewert type were independent predictive factors related to response to DOS(odds ratio=4.631,1.027 and 7.639,respectively;all P<0.05).The nomogram developed with these independent factors showed an excellent performance to predict response to DOS in the TC and VC(C-index:0.838 and 0.824),with area under the receiver operating characteristic curve of 0.838 and 0.824,respectively.The calibration curves showed that the practical and predicted response to DOS effectively coincided.CONCLUSION A novel nomogram developed with pretherapeutic cT stage,GTV and Siewert type predicted the response of Siewert type II/III AEG to NAC with DOS.
文摘BACKGROUND Although papillary thyroid microcarcinoma(PTMC)is not considered a threatening tumor,in some cases,it can be aggressive.Metastatic thrombosis of papillary thyroid carcinoma,follicular thyroid carcinoma,Hürthle cell carcinoma,poorly differentiated thyroid carcinoma and anaplastic thyroid carcinoma have been reported in the literature,but there have been no reports about PTMC.CASE SUMMARY A 45-year-old woman presented with a thyroid mass and thrombosis in a middle thyroid vein during a physical examination.She had no symptoms,and the physical examination showed no positive signs.Subsequent ultrasonographyguided fine-needle aspiration biopsy results indicated an atypical lesion of ambiguous significance,with some actively growing cells(TBSRTCⅢ)and the BRAFV600E mutation not present.This patient underwent left thyroidectomy,isthmus lobectomy,prophylactic central lymph node dissection and thromboembolectomy.Postoperative pathology showed papillary microcarcinoma of the left thyroid,and the thrombus in the middle thyroid vein was a tumor thrombus.CONCLUSION Middle thyroid vein tumor thrombus is an extremely rare condition in PTMC,but it does exist.Lobectomy and thromboembolectomy may be an option for patients with thrombi in the middle vein of the thyroid,and we strongly suggest close follow-up of these patients.
基金Supported by the grant of Youth Fund Projects of Sichuan Provincial Department of Education (No. 11ZB163)
文摘Objective:The aim of the study was to evaluate the inhibition of different chemotherapy drugs on γ-synuclein (SNCG) positive expression of breast cancer cell line MDA-MB231,and the effects on cell cycle and apoptosis,and to explore the related mechanism as well.Methods:We treated the breast cancer cell line MDA-MB231 for the inhibition of SNCG with chemotherapy drugs such as irinotecan,nedaplatin and 5-fluorouracil using RT-PCR and immunohistochemistry,and adopted flow cytometry to detect cell cycle distribution and apoptosis.Results:At the transcription and translation levels,the SNCG expression level in nedaplatin group and 5-fluorouracil group was lower than that of other groups and there was statistically significance (P < 0.01) compared with the control group,while there was not statistically significant between irinotecan group and the control group.After drugs action,cell cycle and distribution in each experiment group changed obviously,where the cells in G0G1 phase increased,especially the cells in the nedaplatin group and 5-fluorouracil group changed most significantly,as well as the obvious change in the cells of nedaplatin group and 5-fluorouracil group in the apoptosis period.Conclusion:There was a stronger inhibition of SNCG expression in nedaplatin and 5-fluorouracil groups,and can cause significant cell cycle and apoptosis changes.It may also be concluded that nedaplatin and 5-fluorouracil could make effects by the mechanisms of inhibiting cancer cell proliferation and inducing cell apoptosis.
文摘The Brain-Computer Interfaces(BCIs)had been proposed and used in therapeutics for decades.However,the need of time-consuming calibration phase and the lack of robustness,which are caused by little-labeled data,are restricting the advance and application of BCI,especially for the BCI based on motor imagery(MI).In this paper,we reviewed the recent development in the machine learning algorithm used in the MI-based BCI,which may provide potential solutions for addressing the issue.We classified these algorithms into two categories,namely,and enhancing the representation and expanding the training set.Specifically,these methods of enhancing the representation of features collected from few EEG trials are based on extracting features of multiple bands,regularization,and so on.The methods of expanding the training dataset include approaches of transfer learning(session to session transfer,subject to subject transfer)and generating artificial EEG data.The result of these techniques showed the resolution of the challenges to some extent.As a developing research area,the study of BCI algorithms in little-labeled data is increasingly requiring the advancement of human brain physiological structure research and more transfer learning algorithms research.
基金Project supported by the National Basic Research Program (973) of China (No.2011CB302203)the Innovation Program of the Science and Technology Commission of Shanghai Municipality,China (No.10511501200)
文摘We synthesize animations from a single image by transferring fluid motion of a video example globally.Given a target image of a fluid scene,an alpha matte is required to extract the fluid region.Our method needs to adjust a user-specified video example for producing the fluid motion suitable for the extracted fluid region.Employing the fluid video database,the flow field of the target image is obtained by warping the optical flow of a video frame that has a visually similar scene to the target image according to their scene correspondences,which assigns fluid orientation and speed automatically.Results show that our method is successful in preserving large fluid features in the synthesized animations.In comparison to existing approaches,it is both possible and useful to utilize our method to create flow animations with higher quality.
