Liquid-liquid phase separation,a novel biochemical phenomenon,has been increasingly studied for its medical applications.It underlies the formation of membrane-less organelles and is involved in many cellular and biol...Liquid-liquid phase separation,a novel biochemical phenomenon,has been increasingly studied for its medical applications.It underlies the formation of membrane-less organelles and is involved in many cellular and biological processes.During transcriptional regulation,dynamic condensates are formed through interactions between transcriptional elements,such as transcription factors,coactivators,and mediators.Cancer is a disease characterized by uncontrolled cell proliferation,but the precise mechanisms underlying tumorigenesis often remain to be elucidated.Emerging evidence has linked abnormal transcriptional condensates to several diseases,especially cancer,implying that phase separation plays an important role in tumorigenesis.Condensates formed by phase separation may have an effect on gene transcription in tumors.In the present review,we focus on the correlation between phase separation and transcriptional regulation,as well as how this phenomenon contributes to cancer development.展开更多
Temporomandibular joint(TMJ)disc displacement is one of the most significant subtypes of temporomandibular joint disorders,but its etiology and mechanism are poorly understood.In this study,we elucidated the mechanism...Temporomandibular joint(TMJ)disc displacement is one of the most significant subtypes of temporomandibular joint disorders,but its etiology and mechanism are poorly understood.In this study,we elucidated the mechanisms by which destruction of inflamed collagen fibrils induces alterations in the mechanical properties and positioning of the TMJ disc.By constructing a rat model of TMJ arthritis,we observed anteriorly dislocated TMJ discs with aggravated deformity in vivo from five weeks to six months after a local injection of Freund’s complete adjuvant.By mimicking inflammatory conditions with interleukin-1 beta in vitro,we observed enhanced expression of collagen-synthesis markers in primary TMJ disc cells cultured in a conventional two-dimensional environment.In contrast,three-dimensional(3D)-cultivated disc cell sheets demonstrated the disordered assembly of inflamed collagen fibrils,inappropriate arrangement,and decreased Young’s modulus.Mechanistically,inflammation-related activation of the nuclear factor kappa-B(NF-κB)pathway occurs during the progression of TMJ arthritis.NF-κB inhibition reduced the collagen fibril destruction in the inflamed disc cell sheets in vitro,and early NF-κB blockade alleviated collagen degeneration and dislocation of the TMJ discs in vivo.Therefore,the NF-κB pathway participates in the collagen remodeling in inflamed TMJ discs,offering a potential therapeutic target for disc displacement.展开更多
This study presents a new boundary element method(BEM)framework for the numerical solution of general time-dependent or transient problems.By reformulating the time derivative as a domain integral,the framework effect...This study presents a new boundary element method(BEM)framework for the numerical solution of general time-dependent or transient problems.By reformulating the time derivative as a domain integral,the framework effectively decouples the treatment of spatial and temporal variables,allowing for the independent application of specialized discretization methods.For the temporal domain,we introduce an innovative time-spectral integration technique,which is based on Gaussian-quadrature-based orthogonal polynomial expansions.This method not only achieves arbitrary orders of accuracy but also significantly enhances computational efficiency and stability,particularly for simulations involving rapid transients or long-time dynamic simulations.The domain integrals in the spatial domain are calculated using the scaled coordinate transformation BEM(SCT-BEM),a mathematically rigorous technique that converts domain integrals into equivalent boundary integrals,preserving the boundary-only discretization advantage inherent in BEM.Numerical experiments on transient heat conduction and dynamic wave propagation further demonstrate the framework’s performance and capabilities.These experiments show that the proposed framework outperforms traditional time-stepping BEM methods,particularly in terms of stability,convergence rates,and computational cost,making it a highly promising tool for practical engineering applications.展开更多
Low power consumption,high responsivity,and self-powering are key objectives for photoelectrochemical ultravio-let detectors.In this research,In-dopedα-Ga_(2)O_(3) nanowire arrays were fabricated on fluorine-doped ti...Low power consumption,high responsivity,and self-powering are key objectives for photoelectrochemical ultravio-let detectors.In this research,In-dopedα-Ga_(2)O_(3) nanowire arrays were fabricated on fluorine-doped tin oxide(FTO)substrates through a hydrothermal approach,with subsequent thermal annealing.These arrays were then used as photoanodes to con-struct a ultraviolet(UV)photodetector.In doping reduced the bandgap ofα-Ga_(2)O_(3),enhancing its absorption of UV light.Conse-quently,the In-dopedα-Ga_(2)O_(3) nanowire arrays exhibited excellent light detection performance.When irradiated by 255 nm deep ultraviolet light,they obtained a responsivity of 38.85 mA/W.Moreover,the detector's response and recovery times are 13 and 8 ms,respectively.The In-dopedα-Ga_(2)O_(3) nanowire arrays exhibit a responsivity that is about three-fold higher than the undoped one.Due to its superior responsivity,the In-doped device was used to develop a photoelectric imaging system.This study demonstrates that dopingα-Ga_(2)O_(3) nanowire with indium is a potent approach for optimizing their photoelectrochemi-cal performance,which also has significant potential for optoelectronic applications.展开更多
Spartina alterniflora's robust reproductive capacity has enabled it to spread rapidly, posing a serious threat to native ecosystems in China. Therefore, accurate quantification of Spartina alterniflora aboveground...Spartina alterniflora's robust reproductive capacity has enabled it to spread rapidly, posing a serious threat to native ecosystems in China. Therefore, accurate quantification of Spartina alterniflora aboveground biomass at a fine scale is crucial for understanding its growth dynamics and managing its invasion. This study focuses on the coastal wetlands of central Jiangsu Province, China, utilizing multispectral unmanned aerial vehicle(UAV) data to map the distribution of Spartina alterniflora. Object-based image analysis(OBIA) combined with support vector machines(SVM) was employed for classification. Additionally, multiple regression models, including univariate, band-based, vegetation index(VI)-based, and multivariate linear regression models integrating both band and VI data, were developed to estimate biomass:(1) the Bands + VIs multiple linear regression model based on fresh weight exhibited the highest estimation accuracy;(2) the optimal model achieved R^(2) values of 0.81 and 0.82 at Dafeng and Tiaozini Nature Reserve,with RMSE values of 591.78 g/m^(2) and 337.62 g/m^(2), and MAE values of 576.82 g/m^(2) and 287.71 g/m^(2), respectively;and(3) the aboveground biomass of Spartina alterniflora primarily ranged from 994.60 g/m^(2) to 5 351.48 g/m^(2) at Dafeng and from 796.05 g/m^(2) to 1 994.02 g/m^(2) in Tiaozini Nature Reserve. These findings highlight the effectiveness of multispectral UAV technology for accurately estimating Spartina alterniflora biomass, providing a robust methodology for wetland vegetation monitoring and invasive species management.展开更多
Dear Editor,In recent years,there has been an increasing focus on the non-motor functions of the cerebellum[1,2].Clinical observations have demonstrated that individuals with cerebellar damage exhibit affective insens...Dear Editor,In recent years,there has been an increasing focus on the non-motor functions of the cerebellum[1,2].Clinical observations have demonstrated that individuals with cerebellar damage exhibit affective insensitivity,executive dysfunction,spatial cognitive impairment,personality changes,and speech difficulties,collectively known as cerebellar cognitive affective syndrome[3].Furthermore,cerebellar dysfunction has been implicated in various neurological disorders such as autism spectrum disorder,obsessive-compulsive disorder,and attention-deficit/hyperactivity disorder[4].展开更多
AIM： To study the in vitro and in vivo inhibitory effects of genistein on invasive potential of Bel 7402 hepatocellular carcinoma （HCC） cells and to explore the underlying mechanism. METHODS： Bel 7402 HCC cells we...AIM： To study the in vitro and in vivo inhibitory effects of genistein on invasive potential of Bel 7402 hepatocellular carcinoma （HCC） cells and to explore the underlying mechanism. METHODS： Bel 7402 HCC cells were exposed to genistein. The invasive activity of tumor cells was assayed in transwell cell culture chamber, p125^FAK expression and cell cycle were evaluated by a functional assay. Cell apoptosis analysis was performed with TUNEL method. In addition, bilateral subrenal capsule xenograft transplantation of HCC was performed in 10 nude mice. Genistein was injected and the invasion of HCC into the renal parenchyma was observed. Nicrovessels with immunohistochemical staining were detected. RESULTS： Genistein significantly inhibited the growth of Bel 7402 cells, the inhibitory rate of tumor cells was 26 -42%. The invasive potential of Bel 7402 cells in vitro was significantly inhibited, the inhibitory rate was 11- 28%. Genistein caused G2/M cell cycle arrest, S phase decreased significantly. The occurrence of apoptosis in genistein group increased significantly. The expression of p125^FAK in 5 μg/mL genistein group （15.26±0.16%） and 10 μg/mL genistein group （12.89±0.36%） was significantly lower than that in the control group （19.75± 1.12%, P〈0.05）. Tumor growth in genistein-treated nude mice was significantly retarded in comparison to control mice, the inhibitory rate of tumor growth was about 20%. Genistein also significantly inhibited the invasion of Bel 7402 cells into the renal parenchyma of nude mice with xenograft transplant. The positive unit value of microvessels in genistein-treated group （10.422 ±0.807） was significantly lower than that in control group （22.330 ± 5.696, P〈 0.01）. CONCLUSION： Genistein can effectively inhibit the invasive potential of Bel 7402 HCC cells by altering cell cycle, apoptosis and angiogenesis, inhibition of focal adhesion kinase may play a significant role in this process.展开更多
AIM： To present our trial using a combination of the human acellular dermal matrix （HADM） implant and an interpositional omentum flap to repair giant abdominal wall defects after extensive tumor resection. METHODS...AIM： To present our trial using a combination of the human acellular dermal matrix （HADM） implant and an interpositional omentum flap to repair giant abdominal wall defects after extensive tumor resection. METHODS： Between February and October of 2007, three patients with giant defects of the abdominal wall after extensive tumor resection underwent reconstruction with a combination of HADN and omentum flap. Postoperative morbidities and signs of herniation were monitored. RESULTS： The abdominal wall reconstruction was successful in these three patients, there was no severe morbidity and no signs of herniation in the follow-up period. CONCLUSION： The combination of HADM and omentum flap offers a new, safe and effective alternative to traditional forms in the repair of giant abdominal wall defects. Further analysis of the long-term outcome and more cases are needed to assess the reliability of this technique.展开更多
AIM： To investigate the inhibitory effects of genistein on metastasis of MHCC97-H hepatocellular carcinoma cells and to explore the underlying mechanism. METHODS： MHCC97-H hepatocellular carcinoma cells were exposed...AIM： To investigate the inhibitory effects of genistein on metastasis of MHCC97-H hepatocellular carcinoma cells and to explore the underlying mechanism. METHODS： MHCC97-H hepatocellular carcinoma cells were exposed to genistein. A cell attachment assay was carried out in a microculture well pre-coated with fibronectin. The invasive activity of tumor cells was assayed in a transwell cell culture chamber, and cell cycle and apoptosis were evaluated by a functional assay. In addition, the expression and phosphorylation of FAK were detected by Western blotting. In situ xenograft transplantation of hepatocellular carcinoma was performed in 12 nude mice and lung metastasis of hepatocellular carcinoma was observed. RESULTS： Genistein significantly inhibited the growth of MHCC97-H cells in vitro. Adhesion and invasiveness of MHCC97-H cells were inhibited in a concentrationdependent fashion, and the inhibitory effect of genistein was more potent in the 10 i^g/mL and 20 i^g/ mL genistein-treated groups. Genistein caused G0/G1 cell cycle arrest, an S phase decrease, and increased apoptosis. The expression and phosphorylation of FAK in MHCC-97H cells were significantly decreased. In situ xenograft transplantation of hepatocellular carcinoma was also significantly suppressed by genistein. The number of pulmonary micrometastatic loci in the genistein group was significantly lower compared with the control group （12.3 ± 1.8 vs 16.6± 2.6, P 〈 0.05）. CONCLUSION： Genistein appears to be a promising agent in the inhibition of metastasis of hepatocellular carcinoma.展开更多
Background The impairment of social function is widespread in the patients with chronic schizophrenia, which seriously affects family, life and work conditions.Aims The main purpose of this study was to investigate th...Background The impairment of social function is widespread in the patients with chronic schizophrenia, which seriously affects family, life and work conditions.Aims The main purpose of this study was to investigate the efficacy of paliperidone in the treatment of social function in chronic schizophrenia.Methods A total of 81 patients who met the standard criteria for schizophrenia and long-term hospitalised inpatients were randomly divided into the treatment group and normal control group following a 1-year prospective follow-up study. The reatment group(41 cases) used paliperidone extended-release tablets for reducing dosage, as appropriate, based on the original treatment strategy; and the control group(40 cases) used the former drugs. All patients were assessed using the Positive and Negative Symptom Scales(PANSS), and the Treatment Emergent Symptom Scale(TESS) was used to assess adverse drug reactions. The Hospitalised Psychiatric Patients' Social Functions Rating Scale(SSPI) was used to assess social function of participants before and after 8 weeks, 6 months and 1 year of treatment.Results At baseline there were no significant differences between the two groups in age, duration of illness, educational background and dosage of antipsychotic drugs(converted into chlorpromazine equivalency). There was statistically significant difference in PANSS positive symptoms by interaction effect(Fgroup×time=18.24, df=3237, p<0.001) and time effect(Ftime=21.66, df=3, p<0.01) and the difference in PANSS positive symptoms by grouping effect(Fgroup=0.68, df=1, p=0.41) was not statistically significant. The difference of grouping effect of PANSS negative symptoms(Fgroup=9.93, df=1, p=0.002), time effect(Ftime=279.15, df=3, p<0.001) and interaction effect(Fgroup×time=279.15, df=3237, p<0.001) were statistically significant. There were statistically significant differences in the grouping effect(Fgroup=6.59, df=1, p=0.012), time effect(Ftime=152.97, df=3, p<0.001) and interaction effect(Fgroup×time=148.82, df=3237, p<0.001) of PANSS general pathological symptoms, the same as the total score of the PANSS, which showed large differences in grouping effect(Fgroup=7.04, df=1, p=0.001), time effect(Ftime=210.78, df=3, p<0.001) and interaction effect(Fgroup×time=205.20, df=3237, p<0.01). We found in the total SSPI score, grouping effect(Fgroup=31.70, df=1, p<0.001), time effect(Ftime=161.84, df=3, p<0.001) and interaction effect(Fgroup×time=132.74, df=3237, p<0.001) were demonstrated to be significantly different. Even though adverse reactionsoccurred 7 times in the treatment group and 44 times in the control group based on the Treatment Emergent Symptom Scale(TESS), incidence rate was significantly lower than that of the control group(χ2=18.854, p<0.001).Conclusion Paliperidone can safely and effectively improve negative symptoms and social function in patients with chronic schizophrenia.展开更多
Photocatalytic process represents a promising approach to overcome the pollution challenge associated with the antibiotics-containing wastewater.This study provides a green,efficient and novel approach to remove cepha...Photocatalytic process represents a promising approach to overcome the pollution challenge associated with the antibiotics-containing wastewater.This study provides a green,efficient and novel approach to remove cephalosporins,particularly cefoperazone sodium(CFP).Bi_(4)O_(5)Br_(2) was chosen for the first time to systematically study its degradation for CFP,including the analysis of material structure,degradation performance,the structure and toxicity of the transformation products,etc.The degradation rate results indicated that Bi_(4)O_(5)Br_(2) had an excellent catalytic activity leading to 78%CFP removal compared with the pure BiOBr(38%)within 120 min of visible light irradiation.In addition,the Bi_(4)O_(5)Br_(2) presents high stability and good organic carbon removal efficiency.The effects of the solution p H(3.12-8.75)on catalytic activity revealed that CFP was mainly photocatalyzed under acidic conditions and hydrolyzed under alkaline conditions.Combined with active species and degradation product identification,the photocatalytic degradation pathways of CFP by Bi_(4)O_(5)Br_(2) was proposed,including hydrolysis,oxidation,reduction and decarboxylation.Most importantly,the identified products were all hydrolysis rather than oxidation byproducts transformed from the intermediate ofβ-lactam bond cleavage in CFP molecule,quite different from the mostly previous studies.Furthermore,the final products were demonstrated to be less toxic through the toxicity analysis.Overall,this study illustrates the detailed mechanism of CFP degradation by Bi_(4)O_(5)Br_(2) and confirms Bi_(4)O_(5)Br_(2) to be a promising material for the photodegradation of CFP.展开更多
Morinda officinalis is a well-known medicinal and edible plant that is widely cultivated in the Lingnan region of southern China.Its dried roots(called bajitian in traditional Chinese medicine)are broadly used to trea...Morinda officinalis is a well-known medicinal and edible plant that is widely cultivated in the Lingnan region of southern China.Its dried roots(called bajitian in traditional Chinese medicine)are broadly used to treat various diseases,such as impotence and rheumatism.Here,we report a high-quality chromosome-scale genome assembly of M.officinalis using Nanopore single-molecule sequencing and Hi-C technology.The assembled genome size was 484.85Mb with a scaffold N50 of 40.97 Mb,and 90.77%of the assembled sequences were anchored on eleven pseudochromosomes.The genome includes 27,698 protein-coding genes,and most of the assemblies are repetitive sequences.Genome evolution analysis revealed that M.officinalis underwent core eudicotγgenome triplication events but no recent whole-genome duplication(WGD).Likewise,comparative genomic analysis showed no large-scale structural variation after species divergence between M.officinalis and Coffea canephora.Moreover,gene family analysis indicated that gene families associated with plant–pathogen interactions and sugar metabolism were significantly expanded in M.officinalis.Furthermore,we identified many candidate genes involved in the biosynthesis of major active components such as anthraquinones,iridoids and polysaccharides.In addition,we also found that the DHQS,GGPPS,TPS-Clin,TPS04,sacA,and UGDH gene families—which include the critical genes for active component biosynthesis—were expanded in M.officinalis.This study provides a valuable resource for understanding M.officinalis genome evolution and active component biosynthesis.This work will facilitate genetic improvement and molecular breeding of this commercially important plant.展开更多
This paper presents a summary of various localized collocation schemes and their engineering applications.The basic concepts of localized collocation methods(LCMs)are first introduced,such as approximation theory,semi...This paper presents a summary of various localized collocation schemes and their engineering applications.The basic concepts of localized collocation methods(LCMs)are first introduced,such as approximation theory,semianalytical collocation methods and localization strategies.Based on these basic concepts,five different formulations of localized collocation methods are introduced,including the localized radial basis function collocation method(LRBFCM)and the generalized finite difference method(GFDM),the localized method of fundamental solutions(LMFS),the localized radial Trefftz collocation method(LRTCM),and the localized collocation Trefftz method(LCTM).Then,several additional schemes,such as the generalized reciprocity method,Laplace and Fourier transformations,and Krylov deferred correction,are introduced to enable the application of the LCM to large-scale engineering and scientific computing for solving inhomogeneous,nonisotropic and time-dependent partial differential equations.Several typical benchmark examples are presented to show the recent developments and applications on the LCM solution of some selected boundary value problems,such as numerical wave flume,potential-based inverse electrocardiography,wave propagation analysis and 2D phononic crystals,elasticity and in-plane crack problems,heat conduction problems in heterogeneous material and nonlinear time-dependent Burgers’equations.Finally,some conclusions and outlooks of the LCMs are summarized.展开更多
p-Menthane type monoterpene derivatives were identified as bio-based compounds with high herbicidal activities. In order to search novel p-menthane type monoterpene derivatives in good performance, a series of novel c...p-Menthane type monoterpene derivatives were identified as bio-based compounds with high herbicidal activities. In order to search novel p-menthane type monoterpene derivatives in good performance, a series of novel cis-p-menthane type Schiff base derivatives were designed and synthesized. All target products were easily available novel compounds and characterized by FT-IR,^1H NMR,^13 C NMR and ESI＋-MS. Their pre-emergence herbicidal activities against annual ryegrass were evaluated. The bioassays indicated that most of the target compounds displayed excellent herbicidal activities in pre-emergence treatment.展开更多
基金supported by the Jiangsu Province Natural Science Foundation(Grant No.BK20201492)the Key Medical Research Project of Jiangsu Provincial Health Commission(Grant No.K2019002)the Clinical Capacity Improvement Project of Jiangsu Province People's Hospital(Grant No.JSPH-MA-2021-8).
文摘Liquid-liquid phase separation,a novel biochemical phenomenon,has been increasingly studied for its medical applications.It underlies the formation of membrane-less organelles and is involved in many cellular and biological processes.During transcriptional regulation,dynamic condensates are formed through interactions between transcriptional elements,such as transcription factors,coactivators,and mediators.Cancer is a disease characterized by uncontrolled cell proliferation,but the precise mechanisms underlying tumorigenesis often remain to be elucidated.Emerging evidence has linked abnormal transcriptional condensates to several diseases,especially cancer,implying that phase separation plays an important role in tumorigenesis.Condensates formed by phase separation may have an effect on gene transcription in tumors.In the present review,we focus on the correlation between phase separation and transcriptional regulation,as well as how this phenomenon contributes to cancer development.
基金supported by the National Natural Science Foundation of China Nos.82370983,81671015(X.W.),82230030(Y.L.),82101043(S.C.)and 82370922(Y.F.)Beijing International Science and Technology Cooperation Project No.Z221100002722003(Y.L.)+4 种基金Beijing Natural Science Foundation Nos.L234017,JL23002(Y.L.),No.7242282(S.C.)and 7232217(Y.G.)Clinical Medicine Plus X-Young Scholars Project of Peking University No.PKU2024LCXQ039(Y.L.)National Program for Multidisciplinary Cooperative Treatment on Major Diseases No.PKUSSNMP-202013(X.W.)Hygiene and Health Development Scientific Research Fostering Plan of Haidian District Beijing No.HP2023-12-509001(J.Z.)Young Clinical Research Fund of the Chinese Stomatological Association No.CSA-02022-03(J.Z.).
文摘Temporomandibular joint(TMJ)disc displacement is one of the most significant subtypes of temporomandibular joint disorders,but its etiology and mechanism are poorly understood.In this study,we elucidated the mechanisms by which destruction of inflamed collagen fibrils induces alterations in the mechanical properties and positioning of the TMJ disc.By constructing a rat model of TMJ arthritis,we observed anteriorly dislocated TMJ discs with aggravated deformity in vivo from five weeks to six months after a local injection of Freund’s complete adjuvant.By mimicking inflammatory conditions with interleukin-1 beta in vitro,we observed enhanced expression of collagen-synthesis markers in primary TMJ disc cells cultured in a conventional two-dimensional environment.In contrast,three-dimensional(3D)-cultivated disc cell sheets demonstrated the disordered assembly of inflamed collagen fibrils,inappropriate arrangement,and decreased Young’s modulus.Mechanistically,inflammation-related activation of the nuclear factor kappa-B(NF-κB)pathway occurs during the progression of TMJ arthritis.NF-κB inhibition reduced the collagen fibril destruction in the inflamed disc cell sheets in vitro,and early NF-κB blockade alleviated collagen degeneration and dislocation of the TMJ discs in vivo.Therefore,the NF-κB pathway participates in the collagen remodeling in inflamed TMJ discs,offering a potential therapeutic target for disc displacement.
基金supported by the National Natural Science Foundation of China(Grant Nos.12372199,12422207,and W2431010)the Natural Science Foundation of Shandong Province of China(Grant No.ZR2021JQ02)the Ningbo Municipal Excellence Research Program(Zhejiang Province,China).
文摘This study presents a new boundary element method(BEM)framework for the numerical solution of general time-dependent or transient problems.By reformulating the time derivative as a domain integral,the framework effectively decouples the treatment of spatial and temporal variables,allowing for the independent application of specialized discretization methods.For the temporal domain,we introduce an innovative time-spectral integration technique,which is based on Gaussian-quadrature-based orthogonal polynomial expansions.This method not only achieves arbitrary orders of accuracy but also significantly enhances computational efficiency and stability,particularly for simulations involving rapid transients or long-time dynamic simulations.The domain integrals in the spatial domain are calculated using the scaled coordinate transformation BEM(SCT-BEM),a mathematically rigorous technique that converts domain integrals into equivalent boundary integrals,preserving the boundary-only discretization advantage inherent in BEM.Numerical experiments on transient heat conduction and dynamic wave propagation further demonstrate the framework’s performance and capabilities.These experiments show that the proposed framework outperforms traditional time-stepping BEM methods,particularly in terms of stability,convergence rates,and computational cost,making it a highly promising tool for practical engineering applications.
基金supported by the National Key Research and Development Program of China(2023YFB3610500)National Natural Science Foundation of China(62104110,62374094)+1 种基金the Project funded by China Postdoctoral Science Foundation(2023T160332)Natural Science Foundation of Nanjing University of Posts and Telecommunications(NY224084,NY224131).
文摘Low power consumption,high responsivity,and self-powering are key objectives for photoelectrochemical ultravio-let detectors.In this research,In-dopedα-Ga_(2)O_(3) nanowire arrays were fabricated on fluorine-doped tin oxide(FTO)substrates through a hydrothermal approach,with subsequent thermal annealing.These arrays were then used as photoanodes to con-struct a ultraviolet(UV)photodetector.In doping reduced the bandgap ofα-Ga_(2)O_(3),enhancing its absorption of UV light.Conse-quently,the In-dopedα-Ga_(2)O_(3) nanowire arrays exhibited excellent light detection performance.When irradiated by 255 nm deep ultraviolet light,they obtained a responsivity of 38.85 mA/W.Moreover,the detector's response and recovery times are 13 and 8 ms,respectively.The In-dopedα-Ga_(2)O_(3) nanowire arrays exhibit a responsivity that is about three-fold higher than the undoped one.Due to its superior responsivity,the In-doped device was used to develop a photoelectric imaging system.This study demonstrates that dopingα-Ga_(2)O_(3) nanowire with indium is a potent approach for optimizing their photoelectrochemi-cal performance,which also has significant potential for optoelectronic applications.
基金Postgraduate Research&Practice Innovation Program of Jiangsu Province under contract Nos KYCX23_1067 and KYCX25_1231the National Natural Science Foundation of China under contract No.42476157。
文摘Spartina alterniflora's robust reproductive capacity has enabled it to spread rapidly, posing a serious threat to native ecosystems in China. Therefore, accurate quantification of Spartina alterniflora aboveground biomass at a fine scale is crucial for understanding its growth dynamics and managing its invasion. This study focuses on the coastal wetlands of central Jiangsu Province, China, utilizing multispectral unmanned aerial vehicle(UAV) data to map the distribution of Spartina alterniflora. Object-based image analysis(OBIA) combined with support vector machines(SVM) was employed for classification. Additionally, multiple regression models, including univariate, band-based, vegetation index(VI)-based, and multivariate linear regression models integrating both band and VI data, were developed to estimate biomass:(1) the Bands + VIs multiple linear regression model based on fresh weight exhibited the highest estimation accuracy;(2) the optimal model achieved R^(2) values of 0.81 and 0.82 at Dafeng and Tiaozini Nature Reserve,with RMSE values of 591.78 g/m^(2) and 337.62 g/m^(2), and MAE values of 576.82 g/m^(2) and 287.71 g/m^(2), respectively;and(3) the aboveground biomass of Spartina alterniflora primarily ranged from 994.60 g/m^(2) to 5 351.48 g/m^(2) at Dafeng and from 796.05 g/m^(2) to 1 994.02 g/m^(2) in Tiaozini Nature Reserve. These findings highlight the effectiveness of multispectral UAV technology for accurately estimating Spartina alterniflora biomass, providing a robust methodology for wetland vegetation monitoring and invasive species management.
基金supported by grants from the STI2030-Major Projects(2021ZD0204000)the Key Strategic Science and Technology Cooperation Project(2023YFE0206800)+1 种基金the Ministry of Science and Technology of China,the National Natural Science Foundation of China(31820103005,32170976,32100791,and 81971874),Zhejiang Province Natural Science Foundation of China(LZ24C090003 and LY21C090003)the Peak Discipline Cultivation Program of Zhejiang University School of Basic Medicine.
文摘Dear Editor,In recent years,there has been an increasing focus on the non-motor functions of the cerebellum[1,2].Clinical observations have demonstrated that individuals with cerebellar damage exhibit affective insensitivity,executive dysfunction,spatial cognitive impairment,personality changes,and speech difficulties,collectively known as cerebellar cognitive affective syndrome[3].Furthermore,cerebellar dysfunction has been implicated in various neurological disorders such as autism spectrum disorder,obsessive-compulsive disorder,and attention-deficit/hyperactivity disorder[4].
基金Supported by the Basic Research Key Project of the Science Foundation of Shanghai Municipal Commission of Science and Technology, No. 02JC14001
文摘AIM： To study the in vitro and in vivo inhibitory effects of genistein on invasive potential of Bel 7402 hepatocellular carcinoma （HCC） cells and to explore the underlying mechanism. METHODS： Bel 7402 HCC cells were exposed to genistein. The invasive activity of tumor cells was assayed in transwell cell culture chamber, p125^FAK expression and cell cycle were evaluated by a functional assay. Cell apoptosis analysis was performed with TUNEL method. In addition, bilateral subrenal capsule xenograft transplantation of HCC was performed in 10 nude mice. Genistein was injected and the invasion of HCC into the renal parenchyma was observed. Nicrovessels with immunohistochemical staining were detected. RESULTS： Genistein significantly inhibited the growth of Bel 7402 cells, the inhibitory rate of tumor cells was 26 -42%. The invasive potential of Bel 7402 cells in vitro was significantly inhibited, the inhibitory rate was 11- 28%. Genistein caused G2/M cell cycle arrest, S phase decreased significantly. The occurrence of apoptosis in genistein group increased significantly. The expression of p125^FAK in 5 μg/mL genistein group （15.26±0.16%） and 10 μg/mL genistein group （12.89±0.36%） was significantly lower than that in the control group （19.75± 1.12%, P〈0.05）. Tumor growth in genistein-treated nude mice was significantly retarded in comparison to control mice, the inhibitory rate of tumor growth was about 20%. Genistein also significantly inhibited the invasion of Bel 7402 cells into the renal parenchyma of nude mice with xenograft transplant. The positive unit value of microvessels in genistein-treated group （10.422 ±0.807） was significantly lower than that in control group （22.330 ± 5.696, P〈 0.01）. CONCLUSION： Genistein can effectively inhibit the invasive potential of Bel 7402 HCC cells by altering cell cycle, apoptosis and angiogenesis, inhibition of focal adhesion kinase may play a significant role in this process.
基金The Key Project of Science and Technology Commission of Shanghai Municipality,No. 074119649
文摘AIM： To present our trial using a combination of the human acellular dermal matrix （HADM） implant and an interpositional omentum flap to repair giant abdominal wall defects after extensive tumor resection. METHODS： Between February and October of 2007, three patients with giant defects of the abdominal wall after extensive tumor resection underwent reconstruction with a combination of HADN and omentum flap. Postoperative morbidities and signs of herniation were monitored. RESULTS： The abdominal wall reconstruction was successful in these three patients, there was no severe morbidity and no signs of herniation in the follow-up period. CONCLUSION： The combination of HADM and omentum flap offers a new, safe and effective alternative to traditional forms in the repair of giant abdominal wall defects. Further analysis of the long-term outcome and more cases are needed to assess the reliability of this technique.
基金Supported by Grants From Shanghai Municipal Health Bureau, No. 054052Shanghai Municipal Commission of Science and Technology, No. 02JC14001
文摘AIM： To investigate the inhibitory effects of genistein on metastasis of MHCC97-H hepatocellular carcinoma cells and to explore the underlying mechanism. METHODS： MHCC97-H hepatocellular carcinoma cells were exposed to genistein. A cell attachment assay was carried out in a microculture well pre-coated with fibronectin. The invasive activity of tumor cells was assayed in a transwell cell culture chamber, and cell cycle and apoptosis were evaluated by a functional assay. In addition, the expression and phosphorylation of FAK were detected by Western blotting. In situ xenograft transplantation of hepatocellular carcinoma was performed in 12 nude mice and lung metastasis of hepatocellular carcinoma was observed. RESULTS： Genistein significantly inhibited the growth of MHCC97-H cells in vitro. Adhesion and invasiveness of MHCC97-H cells were inhibited in a concentrationdependent fashion, and the inhibitory effect of genistein was more potent in the 10 i^g/mL and 20 i^g/ mL genistein-treated groups. Genistein caused G0/G1 cell cycle arrest, an S phase decrease, and increased apoptosis. The expression and phosphorylation of FAK in MHCC-97H cells were significantly decreased. In situ xenograft transplantation of hepatocellular carcinoma was also significantly suppressed by genistein. The number of pulmonary micrometastatic loci in the genistein group was significantly lower compared with the control group （12.3 ± 1.8 vs 16.6± 2.6, P 〈 0.05）. CONCLUSION： Genistein appears to be a promising agent in the inhibition of metastasis of hepatocellular carcinoma.
基金supported in part by the National Science Fund of China(81771450,81471358)Shanghai Health Bureau scientific research grant(201540029)Shanghai Municipal Education Commission-Gaofeng Clinical Medicine Grant(20152530)
文摘Background The impairment of social function is widespread in the patients with chronic schizophrenia, which seriously affects family, life and work conditions.Aims The main purpose of this study was to investigate the efficacy of paliperidone in the treatment of social function in chronic schizophrenia.Methods A total of 81 patients who met the standard criteria for schizophrenia and long-term hospitalised inpatients were randomly divided into the treatment group and normal control group following a 1-year prospective follow-up study. The reatment group(41 cases) used paliperidone extended-release tablets for reducing dosage, as appropriate, based on the original treatment strategy; and the control group(40 cases) used the former drugs. All patients were assessed using the Positive and Negative Symptom Scales(PANSS), and the Treatment Emergent Symptom Scale(TESS) was used to assess adverse drug reactions. The Hospitalised Psychiatric Patients' Social Functions Rating Scale(SSPI) was used to assess social function of participants before and after 8 weeks, 6 months and 1 year of treatment.Results At baseline there were no significant differences between the two groups in age, duration of illness, educational background and dosage of antipsychotic drugs(converted into chlorpromazine equivalency). There was statistically significant difference in PANSS positive symptoms by interaction effect(Fgroup×time=18.24, df=3237, p<0.001) and time effect(Ftime=21.66, df=3, p<0.01) and the difference in PANSS positive symptoms by grouping effect(Fgroup=0.68, df=1, p=0.41) was not statistically significant. The difference of grouping effect of PANSS negative symptoms(Fgroup=9.93, df=1, p=0.002), time effect(Ftime=279.15, df=3, p<0.001) and interaction effect(Fgroup×time=279.15, df=3237, p<0.001) were statistically significant. There were statistically significant differences in the grouping effect(Fgroup=6.59, df=1, p=0.012), time effect(Ftime=152.97, df=3, p<0.001) and interaction effect(Fgroup×time=148.82, df=3237, p<0.001) of PANSS general pathological symptoms, the same as the total score of the PANSS, which showed large differences in grouping effect(Fgroup=7.04, df=1, p=0.001), time effect(Ftime=210.78, df=3, p<0.001) and interaction effect(Fgroup×time=205.20, df=3237, p<0.01). We found in the total SSPI score, grouping effect(Fgroup=31.70, df=1, p<0.001), time effect(Ftime=161.84, df=3, p<0.001) and interaction effect(Fgroup×time=132.74, df=3237, p<0.001) were demonstrated to be significantly different. Even though adverse reactionsoccurred 7 times in the treatment group and 44 times in the control group based on the Treatment Emergent Symptom Scale(TESS), incidence rate was significantly lower than that of the control group(χ2=18.854, p<0.001).Conclusion Paliperidone can safely and effectively improve negative symptoms and social function in patients with chronic schizophrenia.
基金the National Natural Science Foundation of China(Nos.21972073,21677086 and 21577077)Hubei Province Introduces Foreign Talents and Intelligence Projects(No.2019BJH004)+2 种基金China Postdoctoral Science Foundation(No.2018M640721)Postdoctoral Science Foundation of Hubei Province(No.G83)Open Fund of Engineering Research Center of Eco-environment in Three Gorges Reservoir Region(No.KF2019-02)。
文摘Photocatalytic process represents a promising approach to overcome the pollution challenge associated with the antibiotics-containing wastewater.This study provides a green,efficient and novel approach to remove cephalosporins,particularly cefoperazone sodium(CFP).Bi_(4)O_(5)Br_(2) was chosen for the first time to systematically study its degradation for CFP,including the analysis of material structure,degradation performance,the structure and toxicity of the transformation products,etc.The degradation rate results indicated that Bi_(4)O_(5)Br_(2) had an excellent catalytic activity leading to 78%CFP removal compared with the pure BiOBr(38%)within 120 min of visible light irradiation.In addition,the Bi_(4)O_(5)Br_(2) presents high stability and good organic carbon removal efficiency.The effects of the solution p H(3.12-8.75)on catalytic activity revealed that CFP was mainly photocatalyzed under acidic conditions and hydrolyzed under alkaline conditions.Combined with active species and degradation product identification,the photocatalytic degradation pathways of CFP by Bi_(4)O_(5)Br_(2) was proposed,including hydrolysis,oxidation,reduction and decarboxylation.Most importantly,the identified products were all hydrolysis rather than oxidation byproducts transformed from the intermediate ofβ-lactam bond cleavage in CFP molecule,quite different from the mostly previous studies.Furthermore,the final products were demonstrated to be less toxic through the toxicity analysis.Overall,this study illustrates the detailed mechanism of CFP degradation by Bi_(4)O_(5)Br_(2) and confirms Bi_(4)O_(5)Br_(2) to be a promising material for the photodegradation of CFP.
基金supported by a study on the cultivation of the six new varieties of Lingnan Traditional Chinese Medicine(2020B020221001)Scientific Innovation Strategy Construction of the High-level Academy of Agriculture Science(R2019PY-JX003)Southern Medicinal Plants Modern Agricultural Industrial Park of Gaoyao(2018).
文摘Morinda officinalis is a well-known medicinal and edible plant that is widely cultivated in the Lingnan region of southern China.Its dried roots(called bajitian in traditional Chinese medicine)are broadly used to treat various diseases,such as impotence and rheumatism.Here,we report a high-quality chromosome-scale genome assembly of M.officinalis using Nanopore single-molecule sequencing and Hi-C technology.The assembled genome size was 484.85Mb with a scaffold N50 of 40.97 Mb,and 90.77%of the assembled sequences were anchored on eleven pseudochromosomes.The genome includes 27,698 protein-coding genes,and most of the assemblies are repetitive sequences.Genome evolution analysis revealed that M.officinalis underwent core eudicotγgenome triplication events but no recent whole-genome duplication(WGD).Likewise,comparative genomic analysis showed no large-scale structural variation after species divergence between M.officinalis and Coffea canephora.Moreover,gene family analysis indicated that gene families associated with plant–pathogen interactions and sugar metabolism were significantly expanded in M.officinalis.Furthermore,we identified many candidate genes involved in the biosynthesis of major active components such as anthraquinones,iridoids and polysaccharides.In addition,we also found that the DHQS,GGPPS,TPS-Clin,TPS04,sacA,and UGDH gene families—which include the critical genes for active component biosynthesis—were expanded in M.officinalis.This study provides a valuable resource for understanding M.officinalis genome evolution and active component biosynthesis.This work will facilitate genetic improvement and molecular breeding of this commercially important plant.
基金supported by the National Natural Science Foundation of China(Grant Nos.12122205 and 11772119)the Six Talent Peaks Project in Jiangsu Province of China(Grant No.2019-KTHY-009).
文摘This paper presents a summary of various localized collocation schemes and their engineering applications.The basic concepts of localized collocation methods(LCMs)are first introduced,such as approximation theory,semianalytical collocation methods and localization strategies.Based on these basic concepts,five different formulations of localized collocation methods are introduced,including the localized radial basis function collocation method(LRBFCM)and the generalized finite difference method(GFDM),the localized method of fundamental solutions(LMFS),the localized radial Trefftz collocation method(LRTCM),and the localized collocation Trefftz method(LCTM).Then,several additional schemes,such as the generalized reciprocity method,Laplace and Fourier transformations,and Krylov deferred correction,are introduced to enable the application of the LCM to large-scale engineering and scientific computing for solving inhomogeneous,nonisotropic and time-dependent partial differential equations.Several typical benchmark examples are presented to show the recent developments and applications on the LCM solution of some selected boundary value problems,such as numerical wave flume,potential-based inverse electrocardiography,wave propagation analysis and 2D phononic crystals,elasticity and in-plane crack problems,heat conduction problems in heterogeneous material and nonlinear time-dependent Burgers’equations.Finally,some conclusions and outlooks of the LCMs are summarized.
基金funded by the Key Projects in the National Science&Technology Pillar Program during the Twelfth Five-year Plan Period(No.2015BAD15B04)the National Natural Science Foundation of China(No.31600466)the Fundamental Research Funds for Jiangsu Key Laboratory of Biomass Energy and Material(No.JSBEM-S-201605)
文摘p-Menthane type monoterpene derivatives were identified as bio-based compounds with high herbicidal activities. In order to search novel p-menthane type monoterpene derivatives in good performance, a series of novel cis-p-menthane type Schiff base derivatives were designed and synthesized. All target products were easily available novel compounds and characterized by FT-IR,^1H NMR,^13 C NMR and ESI＋-MS. Their pre-emergence herbicidal activities against annual ryegrass were evaluated. The bioassays indicated that most of the target compounds displayed excellent herbicidal activities in pre-emergence treatment.
