Phytochemical investigation of the whole plants of Dicranostigma leptopodum （Maxim） Fedde has lead to the isolation of five alkaloids： dicranostigmine （1）, isocorydinc （2）, corydine （3）, protopine （4） and s...Phytochemical investigation of the whole plants of Dicranostigma leptopodum （Maxim） Fedde has lead to the isolation of five alkaloids： dicranostigmine （1）, isocorydinc （2）, corydine （3）, protopine （4） and sinoacutine （5）. Oxomorphine alkaloid （5） was isolated from the genus Dicranostigma for the first time and the new compound structure （1） was elucidated by various spectroscopic methods including 2D NMR techniques （gCOSY, HMQC, HMBC and NOESY） and HRESI-MS.展开更多
In this study,carbamazepine(CBZ)decay in solution has been studied by coupling electro-coagulation with electro-Fenton(EC-EF)with a novel P-rGO/carbon felt(CF)cathode,aiming to accelerate the in-situ generation of...In this study,carbamazepine(CBZ)decay in solution has been studied by coupling electro-coagulation with electro-Fenton(EC-EF)with a novel P-rGO/carbon felt(CF)cathode,aiming to accelerate the in-situ generation of·OH,instead of adding Fe2+and H2O2.Firstly,the fabri-cated P-rGO and its derived cathode were characterized by XRD,SEM,AFM,XPS and electro-chemical test(EIS,CV and LSV).Secondly,it was confirmed that the performance in removal efficiency and electric energy consumption(EEC)by EC-EF(kobs=0.124 min^(-1),EEC=43.98 kWh/kg CBZ)was better than EF(kobs=0.069 min^(-1),EEC=61.04 kWh/kg CBZ).Then,P-rGO/CF(kobs=0.248 min^(-1),EEC=29.47 kWh/kg CBZ,CE=61.04%)showed the best performance in EC-EF,among all studied heteroatom-doped graphene/CF.This superior performance may be associated with its largest layer spacing and richest C=C,which can promote the electron transfer rate and conductivity of the cathode.Thus,more H2O2 and·OH could be produced to degrade CBZ,and almost 100%CBZ was removed with kobs being 0.337 min^(-1) and the EEC was only 24.18 kWh/kg CBZ,under the optimal conditions(P-rGO loading was 6.0 mg/cm^(2),the current density was 10.0 mA/cm^(2),the gap between electrode was 2.0 cm).Additionally,no matter the influent is acidic,neutral or alkaline,no additional pH adjustment is required for the effluent of EC-EF.At last,an inconsecutive empirical kinetic model was firstly estab-lished to predict the effect of operating parameters on CBZ removal.展开更多
BACKGROUND Esophageal squamous cell carcinoma(ESCC)is a prevalent malignancy with a high morbidity and mortality rate.TMEM100 has been shown to be suppressor gene in a variety of tumors,but there are no reports on the...BACKGROUND Esophageal squamous cell carcinoma(ESCC)is a prevalent malignancy with a high morbidity and mortality rate.TMEM100 has been shown to be suppressor gene in a variety of tumors,but there are no reports on the role of TMEM100 in esophageal cancer(EC).AIM To investigate epigenetic regulation of TMEM100 expression in ESCC and the effect of TMEM100 on ESCC proliferation and invasion.METHODS Firstly,we found the expression of TMEM100 in EC through The Cancer Genome Atlas database.The correlation between TMEM100 gene expression and the survival of patients with EC was further confirmed through Kaplan-Meier analysis.We then added the demethylating agent 5-AZA to ESCC cell lines to explore the regulation of TMEM100 expression by epigenetic modification.To observe the effect of TMEM100 expression on tumor proliferation and invasion by overexpressing TMEM100.Finally,we performed gene set enrichment analysis using the Kyoto Encyclopaedia of Genes and Genomes Orthology-Based Annotation System database to look for pathways that might be affected by TMEM100 and verified the effect of TMEM100 expression on the mitogen-activated protein kinases(MAPK)pathway.RESULTS In the present study,by bioinformatic analysis we found that TMEM100 was lowly expressed in EC patients compared to normal subjects.Kaplan-meier survival analysis showed that low expression of TMEM100 was associated with poor prognosis in patients with EC.Then,we found that the demethylating agent 5-AZA resulted in increased expression of TMEM100 in ESCC cells[quantitative real-time PCR(qRT-PCR)and western blotting].Subsequently,we confirmed that overexpression of TMEM100 leads to its increased expression in ESCC cells(qRT-PCR and western blotting).Overexpression of TMEM100 also inhibited proliferation,invasion and migration of ESCC cells(cell counting kit-8 and clone formation assays).Next,by enrichment analysis,we found that the gene set was significantly enriched in the MAPK signaling pathway.The involvement of TMEM100 in the regulation of MAPK signaling pathway in ESCC cell was subsequently verified by western blotting.CONCLUSION TMEM100 is a suppressor gene in ESCC,and its low expression may lead to aberrant activation of the MAPK pathway.Promoter methylation may play a key role in regulating TMEM100 expression.展开更多
In this study,an Escherichia coli(E.coli)whole-cell biosensor for the specific detection of bioavailable arsenic was developed by placing a green fluorescent protein(GFP)reporter gene under the control of the ArsR1(GS...In this study,an Escherichia coli(E.coli)whole-cell biosensor for the specific detection of bioavailable arsenic was developed by placing a green fluorescent protein(GFP)reporter gene under the control of the ArsR1(GSU2952)regulatory circuit from Geobacter sulfurreducens.E.coli cells only emitted green fluorescence in the presence of arsenite and were more sensitive to arsenite when they were grown in M9 supplemented medium compared to LB medium.Under optimal test conditions,the Geobacter arsR1 promoter had a detection limit of 0.01 mM arsenite and the GFP expression was linear within a range of 0.03-0.1 mM(2.25-7.5 mg/l).These values were well below World Health Organization’s drinking water quality standard,which is 10 mg/l.The feasibility of using this whole-cell biosensor to detect arsenic in water samples,such as arsenic polluted tap water and landfill leachate was verified.The biosensor was determined to be just as sensitive as atomic fluorescence spectrometry.This study examines the potential applications of biosensors constructed with Geobacter ArsR-Pars regulatory circuits and provides a rapid and cost-effective tool that can be used for arsenic detection in water samples.展开更多
The thermotolerant yeast Kluyveromyces marxianus is known for its potential in high-temperature ethanol fermentation,yet it suffers from excess acetic acid production at elevated temperatures,which hinders ethanol pro...The thermotolerant yeast Kluyveromyces marxianus is known for its potential in high-temperature ethanol fermentation,yet it suffers from excess acetic acid production at elevated temperatures,which hinders ethanol production.To better understand how the yeast responds to acetic acid stress during high-temperature ethanol fermentation,this study investigated its transcriptomic changes under this condition.RNA sequencing(RNA-seq)was used to identify differentially expressed genes(DEGs)and enriched gene ontology(GO)terms and pathways under acetic acid stress.The results showed that 611 genes were differentially expressed,and GO and pathway enrichment analysis revealed that acetic acid stress promoted protein catabolism but repressed protein synthesis during high-temperature fermentation.Protein-protein interaction(PPI)networks were also constructed based on the interactions between proteins coded by the DEGs.Hub genes and key modules in the PPI networks were identified,providing insight into the mechanisms of this yeast’s response to acetic acid stress.The findings suggest that the decrease in ethanol production is caused by the imbalance between protein catabolism and protein synthesis.Overall,this study provides valuable insights into the mechanisms of K.marxianus’s response to acetic acid stress and highlights the importance of maintaining a proper balance between protein catabolism and protein synthesis for high-temperature ethanol fermentation.展开更多
基金supported by the"Hundred Talents Program"and"West Light Program"of Chinese Academy of Sciences and the National Natural Sciences Foundation of China(No.20775083)
文摘Phytochemical investigation of the whole plants of Dicranostigma leptopodum （Maxim） Fedde has lead to the isolation of five alkaloids： dicranostigmine （1）, isocorydinc （2）, corydine （3）, protopine （4） and sinoacutine （5）. Oxomorphine alkaloid （5） was isolated from the genus Dicranostigma for the first time and the new compound structure （1） was elucidated by various spectroscopic methods including 2D NMR techniques （gCOSY, HMQC, HMBC and NOESY） and HRESI-MS.
基金This work was carried out with the support of the Fundamental Research Funds for the Central Universities(No.2017PT01)the Beijing Natural Science Foundation(No.L182027)+2 种基金the National Key Research and Development Program of China(No.2021YFE0100800)the National Natural Science Foundation of China(Nos.22076012 and 51878047)the Beijing Forestry University Outstanding Young Talent Cultivation Project(No.2019JQ03008).
文摘In this study,carbamazepine(CBZ)decay in solution has been studied by coupling electro-coagulation with electro-Fenton(EC-EF)with a novel P-rGO/carbon felt(CF)cathode,aiming to accelerate the in-situ generation of·OH,instead of adding Fe2+and H2O2.Firstly,the fabri-cated P-rGO and its derived cathode were characterized by XRD,SEM,AFM,XPS and electro-chemical test(EIS,CV and LSV).Secondly,it was confirmed that the performance in removal efficiency and electric energy consumption(EEC)by EC-EF(kobs=0.124 min^(-1),EEC=43.98 kWh/kg CBZ)was better than EF(kobs=0.069 min^(-1),EEC=61.04 kWh/kg CBZ).Then,P-rGO/CF(kobs=0.248 min^(-1),EEC=29.47 kWh/kg CBZ,CE=61.04%)showed the best performance in EC-EF,among all studied heteroatom-doped graphene/CF.This superior performance may be associated with its largest layer spacing and richest C=C,which can promote the electron transfer rate and conductivity of the cathode.Thus,more H2O2 and·OH could be produced to degrade CBZ,and almost 100%CBZ was removed with kobs being 0.337 min^(-1) and the EEC was only 24.18 kWh/kg CBZ,under the optimal conditions(P-rGO loading was 6.0 mg/cm^(2),the current density was 10.0 mA/cm^(2),the gap between electrode was 2.0 cm).Additionally,no matter the influent is acidic,neutral or alkaline,no additional pH adjustment is required for the effluent of EC-EF.At last,an inconsecutive empirical kinetic model was firstly estab-lished to predict the effect of operating parameters on CBZ removal.
文摘BACKGROUND Esophageal squamous cell carcinoma(ESCC)is a prevalent malignancy with a high morbidity and mortality rate.TMEM100 has been shown to be suppressor gene in a variety of tumors,but there are no reports on the role of TMEM100 in esophageal cancer(EC).AIM To investigate epigenetic regulation of TMEM100 expression in ESCC and the effect of TMEM100 on ESCC proliferation and invasion.METHODS Firstly,we found the expression of TMEM100 in EC through The Cancer Genome Atlas database.The correlation between TMEM100 gene expression and the survival of patients with EC was further confirmed through Kaplan-Meier analysis.We then added the demethylating agent 5-AZA to ESCC cell lines to explore the regulation of TMEM100 expression by epigenetic modification.To observe the effect of TMEM100 expression on tumor proliferation and invasion by overexpressing TMEM100.Finally,we performed gene set enrichment analysis using the Kyoto Encyclopaedia of Genes and Genomes Orthology-Based Annotation System database to look for pathways that might be affected by TMEM100 and verified the effect of TMEM100 expression on the mitogen-activated protein kinases(MAPK)pathway.RESULTS In the present study,by bioinformatic analysis we found that TMEM100 was lowly expressed in EC patients compared to normal subjects.Kaplan-meier survival analysis showed that low expression of TMEM100 was associated with poor prognosis in patients with EC.Then,we found that the demethylating agent 5-AZA resulted in increased expression of TMEM100 in ESCC cells[quantitative real-time PCR(qRT-PCR)and western blotting].Subsequently,we confirmed that overexpression of TMEM100 leads to its increased expression in ESCC cells(qRT-PCR and western blotting).Overexpression of TMEM100 also inhibited proliferation,invasion and migration of ESCC cells(cell counting kit-8 and clone formation assays).Next,by enrichment analysis,we found that the gene set was significantly enriched in the MAPK signaling pathway.The involvement of TMEM100 in the regulation of MAPK signaling pathway in ESCC cell was subsequently verified by western blotting.CONCLUSION TMEM100 is a suppressor gene in ESCC,and its low expression may lead to aberrant activation of the MAPK pathway.Promoter methylation may play a key role in regulating TMEM100 expression.
基金supported by the Fundamental Research Funds for the Central Universities[grant numbers BLX201934,2019ZY19]Beijing Municipal Education Commission through Innovative Transdisciplinary Program“Ecological Restoration Engineering”.
文摘In this study,an Escherichia coli(E.coli)whole-cell biosensor for the specific detection of bioavailable arsenic was developed by placing a green fluorescent protein(GFP)reporter gene under the control of the ArsR1(GSU2952)regulatory circuit from Geobacter sulfurreducens.E.coli cells only emitted green fluorescence in the presence of arsenite and were more sensitive to arsenite when they were grown in M9 supplemented medium compared to LB medium.Under optimal test conditions,the Geobacter arsR1 promoter had a detection limit of 0.01 mM arsenite and the GFP expression was linear within a range of 0.03-0.1 mM(2.25-7.5 mg/l).These values were well below World Health Organization’s drinking water quality standard,which is 10 mg/l.The feasibility of using this whole-cell biosensor to detect arsenic in water samples,such as arsenic polluted tap water and landfill leachate was verified.The biosensor was determined to be just as sensitive as atomic fluorescence spectrometry.This study examines the potential applications of biosensors constructed with Geobacter ArsR-Pars regulatory circuits and provides a rapid and cost-effective tool that can be used for arsenic detection in water samples.
基金supported by the National Undergraduate Training Program for Innovation and Entrepreneurship(202110022074,202198039)Beijing Municipal Education Commission through the Innovative Transdisciplinary Program"Ecological Restoration Engineering".
文摘The thermotolerant yeast Kluyveromyces marxianus is known for its potential in high-temperature ethanol fermentation,yet it suffers from excess acetic acid production at elevated temperatures,which hinders ethanol production.To better understand how the yeast responds to acetic acid stress during high-temperature ethanol fermentation,this study investigated its transcriptomic changes under this condition.RNA sequencing(RNA-seq)was used to identify differentially expressed genes(DEGs)and enriched gene ontology(GO)terms and pathways under acetic acid stress.The results showed that 611 genes were differentially expressed,and GO and pathway enrichment analysis revealed that acetic acid stress promoted protein catabolism but repressed protein synthesis during high-temperature fermentation.Protein-protein interaction(PPI)networks were also constructed based on the interactions between proteins coded by the DEGs.Hub genes and key modules in the PPI networks were identified,providing insight into the mechanisms of this yeast’s response to acetic acid stress.The findings suggest that the decrease in ethanol production is caused by the imbalance between protein catabolism and protein synthesis.Overall,this study provides valuable insights into the mechanisms of K.marxianus’s response to acetic acid stress and highlights the importance of maintaining a proper balance between protein catabolism and protein synthesis for high-temperature ethanol fermentation.
