Studies on plant diversity are usually based on the total number of species in a community.However,few studies have examined species richness(SR)of different plant life forms in a community along largescale environmen...Studies on plant diversity are usually based on the total number of species in a community.However,few studies have examined species richness(SR)of different plant life forms in a community along largescale environmental gradients.Particularly,the relative importance(RIV)of different plant life forms in a community and how they vary with environmental variables are still unclear.To fill these gaps,we determined plant diversity of ephemeral plants,annual herbs,perennial herbs,and woody plants from 187 sites across drylands in China.The SR patterns of herbaceous plants,especially perennial herbs,and their RIV in plant communities increased with increasing precipitation and soil nutrient content;however,the RIV of annual herbs was not altered along these gradients.The SR and RIV of ephemeral plants were affected mainly by precipitation seasonality.The SR of woody plants had a unimodal relationship with air temperature and exhibited the highest RIV and SR percentage in plant communities under the harshest environments.An obvious shift emerged in plant community composition,SR and their critical impact factors at 238.5 mm of mean annual precipitation(MAP).In mesic regions(>238.5 mm),herbs were the dominant species,and the SR displayed a relatively slow decreasing rate with increasing aridity,which was mediated mainly by MAP and soil nutrients.In arid regions(<238.5 mm),woody plants were the dominant species,and the SR displayed a relatively fast decreasing rate with increasing aridity,which was mediated mainly by climate variables,especially precipitation.Our findings highlight the importance of comparative life form studies in community structure and biodiversity,as their responses to gradients differed substantially on a large scale.展开更多
Background:The forest musk deer,a rare fauna species found in China,is famous for its musk secretion which is used in selected Traditional Chinese medicines.However,over-hunting has led to musk deer becoming an endang...Background:The forest musk deer,a rare fauna species found in China,is famous for its musk secretion which is used in selected Traditional Chinese medicines.However,over-hunting has led to musk deer becoming an endangered species,and their survival is also greatly challenged by various high incidence and high mortality respiratory and intestinal diseases such as septic pneumonia and enteritis.Accumulating evidence has demonstrated that Akkermannia muciniphila(AKK)is a promising probiotic,and we wondered whether AKK could be used as a food additive in animal breeding pro-grammes to help prevent intestinal diseases.Methods:We isolated one AKK strain from musk deer feces(AKK-D)using an im-proved enrichment medium combined with real-time PCR.After confirmation by 16S rRNA gene sequencing,a series of in vitro tests was conducted to evaluate the probiotic effects of AKK-D by assessing its reproductive capability,simulated gas-trointestinal fluid tolerance,acid and bile salt resistance,self-aggregation ability,hy-drophobicity,antibiotic sensitivity,hemolysis,harmful metabolite production,biofilm formation ability,and bacterial adhesion to gastrointestinal mucosa.Results:The AKK-D strain has a probiotic function similar to that of the standard strain in humans(AKK-H).An in vivo study found that AKK-D significantly amelio-rated symptoms in the enterotoxigenic Escherichia coli(ETEC)-induced murine diar-rhea model.AKK-D improved organ damage,inhibited inflammatory responses,and improved intestinal barrier permeability.Additionally,AKK-D promoted the reconsti-tution and maintenance of the homeostasis of gut microflora,as indicated by the fact that AKK-D-treated mice showed a decrease in Bacteroidetes and an increase in the proportion of other beneficial bacteria like Muribaculaceae,Muribaculum,and unclas-sified f_Lachnospiaceae compared with the diarrhea model mice.Conclusion:Taken together,our data show that this novel AKK-D strain might be a potential probiotic for use in musk deer breeding,although further extensive system-atic research is still needed.展开更多
MicroRNAs(miRNAs)are abundant in the brain and mounting evidence suggests their involvement in the critical processes such as neurodevelopment,synaptic plasticity,and the development of neurodegenerative diseases.Thus...MicroRNAs(miRNAs)are abundant in the brain and mounting evidence suggests their involvement in the critical processes such as neurodevelopment,synaptic plasticity,and the development of neurodegenerative diseases.Thus,miRNAs may be promising therapeutic drugs for the treatment of neurodegenerative disorders.However,naked miRNAs are not able to enter cells directly,especially brain cells.Therefore,suitable carriers for safe and efficient miRNA delivery to brain cells are of great importance.Chitosan nanoparticles,with the excellent properties such as good compatibility and brilliant degradability,may act as a promising carrier for miRNA drug delivery.In this study,chitosan nanoparticles were prepared and their properties such as particle size,zeta potential and encapsulation efficiency were optimized to encapsulate miRNAs.The delivery efficiency of miRNA-loaded nanoparticles was then evaluated in both neuronal and microglia cells.The results demonstrated chitosan nanoparticles encapsulated miRNAs efficiently and showed excellent sustained releasing in vitro.Moreover,chitosan nanoparticles delivered miRNA to both neurons and microglia with very low toxicity and high efficiency.In conclusion,chitosan nanoparticles are promising carriers for the delivery of miRNAs to brain cells,which may be used for the early intervention and treatment of neurodegenerative disorders.展开更多
The extracellular vesicles show great potential as a noninvasive biomarker for the early detection of cancer.Hence,there is an urgent requirement to create biosensors that are time-saving,simple,and easily scalable in...The extracellular vesicles show great potential as a noninvasive biomarker for the early detection of cancer.Hence,there is an urgent requirement to create biosensors that are time-saving,simple,and easily scalable in order to accomplish rapid,sensitive,and quantitative detection of extracellular vesicles.In this study,we present a self-propelled DNA walker powered by endonuclease Nt.Bbv CI,which enables the development of a“signal on”sensing platform for the rapid and highly sensitive detection of extracellular vesicles.The DNA motor employed tracks made of streptavidin magnetic beads,which consisted of substrate strands labeled with fluorescein and motor strands locked by aptamers.The aptamer recognition of the target protein on extracellular vesicles unlocked the motor strand,initiating the DNA motor process.After replacing the optimal buffer solution containing the endonuclease Nt.BbvC I,the motor strands autonomously moved along the streptavidin magnetic beads track,continuously releasing fluorescent molecules and producing detectable fluorescence signals.Under optimal conditions,the detection range was from 2×10~4particles/mL to 2×10~9particles/mL,with a detection limit of 2.9×10~3particles/mL,demonstrating excellent selectivity.This method has demonstrated good selectivity in different tumorderived extracellular vesicles and performs well in complex biological samples.The ability to effectively analyze surface proteins of extracellular vesicles in a short period of time gives our DNA walker a tremendous potential for developing simple and cost-effective clinical diagnostic devices.展开更多
Objective:To evaluate the global liter-ature output on the relationship between brain-derived neurotrophic factor(BDNF)and cognitive function in al-cohol dependence syndrome using bibliometric methods and explore the ...Objective:To evaluate the global liter-ature output on the relationship between brain-derived neurotrophic factor(BDNF)and cognitive function in al-cohol dependence syndrome using bibliometric methods and explore the status and trends in this field.Method:The literature on the application of BDNF in cognitive impairment caused by alcohol dependence syndrome published from 1995 to 2023 were retrieved from Web of Science,and the relevant information(publication characteristics,country and institution,author,number of publications,citation,journal and research field,corresponding author,key words,etc.)was recorded.The bibliometrix R package was used for quantitative and qualitative analysis of publication output and author contributions.Result:A total of 99 articles were included.The overall number of publications in this field has increased over time.The countries and institutions that contributed the most to the field were the United States and the Academy of Medical Sciences of Iranian universities,respectively.Most of the authors were from the United States,followed by Spain,China,and Iran.Ceccanti M,Fiore M were the most productive authors.Publications with Ceccanti M had the highest h-index.The most cited reference author is Haenninen H(227 citations),and the number one published journal is Alcohol.Most articles were published in 2020(n=12)and 2022(n=11),followed by 2019 and 2021(n=10).The corresponding author has the largest number of publications from the United States,and more publications from a single country tend to have more cooperation from other countries.BDNF and alcohol appeared more frequently in various keyword clouds.However,significant differences remained in the author keyword cloud,keyword plus word cloud,and paper topic word cloud.Conclusion:BDNF has great potential in the application of cognitive dysfunction caused by alcohol dependence syndrome.Bibliometric methods and data visualization techniques can help understand the current state of research progress and enable relevant scholars and practitioners to predict the development trends in this field.展开更多
A novel approach was developed to fabricate a label-free electrochemical aptasensor for specific detection of mercury ions(Hg^(2+)).This involved modifying polylysine(PLL)-coated black phosphorus-porous graphene(BP-PG...A novel approach was developed to fabricate a label-free electrochemical aptasensor for specific detection of mercury ions(Hg^(2+)).This involved modifying polylysine(PLL)-coated black phosphorus-porous graphene(BP-PG)nanocomposites(PLL/BP-PG)onto the surface of glassy carbon electrodes(GCE),which were further modified with gold nanoparticles(AuNPs)to combine with a thiolated aptamer(Apt)capable of specifically recognizing Hg^(2+).BP-PG was synthesized using the solvothermal method and covalently bonded to form BP-PG nanosheets,resulting in significant enhanced electrochemical properties of the PLL/BP-PG composite.Furthermore,the PLL/BP-PG composite was improved environmental stability of BP and provided a considerable quantity of-NH_(2)for bonding to Au NPs firmly by assembling.The physical properties and electrochemical behavior of the substrate materials were investigated using various characterization techniques,and analytical parameters were optimized.It is shown that,the Apt/AuNPs/PLL/BP-PG/GCE had a linear response(R~2=0.999)with good selectivity and high sensitivity over the Hg^(2+)range of 1-10,000 nmol/L.The proposed sensor has a detection limit of 0.045 nmol/L and can be employed for detecting of Hg^(2+).It also obtained satisfying results in river water,soil and vegetable samples.展开更多
High-voltage battery systems bring significant increases in energy density but are also accompanied by fast degradation of electrochemical performance and serious safety issues.Herein,Li^(+)coordination structure regu...High-voltage battery systems bring significant increases in energy density but are also accompanied by fast degradation of electrochemical performance and serious safety issues.Herein,Li^(+)coordination structure regulation was conducted to formulate a non-flammable electrolyte,which consists of 1.5 M lithium bis(fluor sulfonyl)imide(LiFSI)in triethyl phosphate and methyl 2,2,2-trifluoromethyl carbonate(FEMC).The renamed TEP-FEMC-FEC(TFF)electrolyte exhibits an FSI^(−)-dominated solvation structure contributed by the weakly-solvating ability of FEMC.The generated inorganic-rich interfacial layers are conducive to stabilizing the phase transition of high-voltage cathodes while suppressing the dendritic growth on lithium metal or co-intercalation behavior in graphite anode.This TFF electrolyte enables LiCoO_(2)||Li batteries to achieve capacity maintenance over 79%after 400 cycles with high-rate of 5 C at an ultra-high voltage of 4.6 V,and an outstanding capacity exceeding 100 mA h g^(−1)even at a super-high current density of 20 C.Additionally,the Ah-level LiCoO_(2)||graphite pouch cells also exhibit high capacity retention and satisfactory safety performance even under fast charging.This work provides a novel research direction for the pursuit of high energy density non-flammable electrolytes.展开更多
Vegetable fields are often contaminated by heavy metals,and Spodoptera exigua is a major vegetable pest which is stressed by heavy metals mainly by feeding.In this study,cadmium accumulation in the tissues of S.exigua...Vegetable fields are often contaminated by heavy metals,and Spodoptera exigua is a major vegetable pest which is stressed by heavy metals mainly by feeding.In this study,cadmium accumulation in the tissues of S.exigua exposed to cadmium and its effects on the growth and development of the parents and the offspring were investigated.Under the stress of different concentrations of cadmium(0.2,3.2,and 51.2 mg kg^(-1)),the cadmium content in each tissue of S.exigua increased in a dose-dependent manner.At the larval stage,the highest cadmium accumulation was found in midgut in all three cadmium treatments,but at the adult stage,the highest cadmium content was found in fat body.In addition,the cadmium content in ovaries was much higher than in testes.When F1S.exigua was stressed by cadmium and the F_(2)generation was not fed a cadmium-containing diet,the larval survival,pupation rate,emergence rate and fecundity of the F_(2)generation were significantly reduced in the 51.2 mg kg^(-1)treatment compared to the corresponding F1generation.Even in the F_(2)generation of the 3.2 mg kg^(-1)treatment,the fecundity was significantly lower than in the parental generation.The fecundity of the only-female stressed treatment was significantly lower than that of the only-male stressed treatment at the 3.2 and 51.2 mg kg^(-1)cadmium exposure levels.When only mothers were stressed at the larval stage,the fecundity of the F_(2)generation was significantly lower than that of the F1generation in the 51.2 mg kg^(-1)treatment,and it was also significantly lower than in the 3.2 and 0.2 mg kg^(-1)treatments.The results of our study can provide useful information for forecasting the population increase trends under different heavy metal stress conditions and for the reliable environmental risk assessment of heavy metal pollution.展开更多
Although it has been developed for many years, nucleic acid aptamer screening technology still fails to be widely used, a considerable part of it is due to the variability of tumor cell morphology, which leads to the ...Although it has been developed for many years, nucleic acid aptamer screening technology still fails to be widely used, a considerable part of it is due to the variability of tumor cell morphology, which leads to the use of immortalized cell lines in the laboratory to screen nucleic acid aptamers for recognition ability of tumor cells in the diseased body.To address this, primary cells that can be stably passaged were isolated and extracted from spontaneous tumors of genetically engineered pancreatic ductal adenocarcinoma model mice in this study.Next, an automated screening instrument for nucleic acid aptamers developed autonomously by our group was used to perform efficient aptamer screening using a limited number of cells, and the obtained nucleic acid aptamers were affinity verified at the cellular level.Finally, to answer the question of the cell growth environment difference on the recognition ability of nucleic acid aptamers, we verified its targeting ability to tumors in vivo on a nude mice xenograft tumor model, and further used a common antitumor drug doxorubicin combined with nucleic acid aptamers to verify the drug loading ability of this aptamer combined with the targeting therapeutic ability.展开更多
The outbreak of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)in late 2019 has negatively affected people's lives and productivity.Because the mode of transmission of SARS-CoV-2 is of great concern,th...The outbreak of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)in late 2019 has negatively affected people's lives and productivity.Because the mode of transmission of SARS-CoV-2 is of great concern,this review discusses the sources of virus aerosols and possible transmission routes.First,we discuss virus aerosol collection methods,including natural sedimentation,solid impact,liquid impact,centrifugal,cyclone and electrostatic adsorption methods.Then,we review common virus aerosol detection methods,including virus culture,metabolic detection,nucleic acid-based detection and immunology-based detection methods.Finally,possible solutions for the detection of SARS-CoV-2 aerosols are introduced.Point-of-care testing has long been a focus of attention.In the near future,the development of an instrument that integrates sampling and output results will enable the real-time,automatic monitoring of patients.展开更多
Cytokinin is a critical growth regulator for various aspects of plant growth and development. In Arabidopsis, cytokinin signaling is mediated by a two-component system-based phosphorelay that transmits a signal from t...Cytokinin is a critical growth regulator for various aspects of plant growth and development. In Arabidopsis, cytokinin signaling is mediated by a two-component system-based phosphorelay that transmits a signal from the receptors, through histidine phosphotransfer proteins, to the downstream response regulators （ARRs）. Of these ARRs, type-A ARR genes, whose transcription can be rapidly induced by cytokinin, act as negative regulators of eytokinin signaling. However, because of functional redundancy, the function of type-A ARR genes in plant growth and development is not well understood by analyzing loss-of-function mutants. In this study, we performed a comparative functional study on all ten type-A ARR genes by analyzing transgenic plants overexpressing these ARR genes fused to a MYC epitope tag. Overexpression of ARR genes results in a variety of cytokinin-associated phenotypes. Notably, overexpression of different ARR transgenes causes diverse phenotypes, even between phylogenetically closely-related gene pairs, such as within the ARR3-ARR4 and ARR5-ARR6 pairs. We found that the accumulation of a subset of ARR proteins （ARR3, ARR5, ARR7, ARR16 and ARR17; possibly ARR8 and ARR15） is increased by MG132, a specific proteasomal inhibitor, indicating that stability of these proteins is regulated by proteasomal degradation. Moreover, similar to that of previously characterized ARR5, ARR6 and ARR7, stability of ARR16 and ARR17, possibly including ARR8 and ARR15, is regulated by cytokinin. These results suggest that type-A ARR proteins are regulated by a combinatorial mechanism involving both the cytokinin and proteasome pathways, thereby executing distinctive functions in plant growth and development.展开更多
Tissue engineering has become a promising strategy for repairing damaged cartilage and bone tissue. Among the scaffolds for tissue-engineering applications, injectable hydrogels have demonstrated great potential for u...Tissue engineering has become a promising strategy for repairing damaged cartilage and bone tissue. Among the scaffolds for tissue-engineering applications, injectable hydrogels have demonstrated great potential for use as three-dimensional cell culture scaffolds in cartilage and bone tissue engineering, owing to their high water content, similarity to the natural extracellular matrix(ECM), porous framework for cell transplantation and proliferation, minimal invasive properties, and ability to match irregular defects. In this review, we describe the selection of appropriate biomaterials and fabrication methods to prepare novel injectable hydrogels for cartilage and bone tissue engineering. In addition, the biology of cartilage and the bony ECM is also summarized. Finally, future perspectives for injectable hydrogels in cartilage and bone tissue engineering are discussed.展开更多
基金supported by the National Key Research and Development Program of China(2023YFF0805602)National Natural Science Foundation of China(32225032,32001192,32271597)+1 种基金the Innovation Base Project of Gansu Province(2021YFF0703904)the Science and Technology Program of Gansu Province(24JRRA515,22JR5RA525,23JRRA1157).
文摘Studies on plant diversity are usually based on the total number of species in a community.However,few studies have examined species richness(SR)of different plant life forms in a community along largescale environmental gradients.Particularly,the relative importance(RIV)of different plant life forms in a community and how they vary with environmental variables are still unclear.To fill these gaps,we determined plant diversity of ephemeral plants,annual herbs,perennial herbs,and woody plants from 187 sites across drylands in China.The SR patterns of herbaceous plants,especially perennial herbs,and their RIV in plant communities increased with increasing precipitation and soil nutrient content;however,the RIV of annual herbs was not altered along these gradients.The SR and RIV of ephemeral plants were affected mainly by precipitation seasonality.The SR of woody plants had a unimodal relationship with air temperature and exhibited the highest RIV and SR percentage in plant communities under the harshest environments.An obvious shift emerged in plant community composition,SR and their critical impact factors at 238.5 mm of mean annual precipitation(MAP).In mesic regions(>238.5 mm),herbs were the dominant species,and the SR displayed a relatively slow decreasing rate with increasing aridity,which was mediated mainly by MAP and soil nutrients.In arid regions(<238.5 mm),woody plants were the dominant species,and the SR displayed a relatively fast decreasing rate with increasing aridity,which was mediated mainly by climate variables,especially precipitation.Our findings highlight the importance of comparative life form studies in community structure and biodiversity,as their responses to gradients differed substantially on a large scale.
基金This research was funded by Shaanxi Administration of Traditional Chinese Medicine Projects(2021-QYZL-01,2021-QYPT-001)Key R&D Project in Shaanxi Province(2023-YBSF-463)+1 种基金the Foundation of Science and Technology in Shaanxi Province(2020TD-050)the Fundamental Research Funds for the Central Universities(GK202205010).
文摘Background:The forest musk deer,a rare fauna species found in China,is famous for its musk secretion which is used in selected Traditional Chinese medicines.However,over-hunting has led to musk deer becoming an endangered species,and their survival is also greatly challenged by various high incidence and high mortality respiratory and intestinal diseases such as septic pneumonia and enteritis.Accumulating evidence has demonstrated that Akkermannia muciniphila(AKK)is a promising probiotic,and we wondered whether AKK could be used as a food additive in animal breeding pro-grammes to help prevent intestinal diseases.Methods:We isolated one AKK strain from musk deer feces(AKK-D)using an im-proved enrichment medium combined with real-time PCR.After confirmation by 16S rRNA gene sequencing,a series of in vitro tests was conducted to evaluate the probiotic effects of AKK-D by assessing its reproductive capability,simulated gas-trointestinal fluid tolerance,acid and bile salt resistance,self-aggregation ability,hy-drophobicity,antibiotic sensitivity,hemolysis,harmful metabolite production,biofilm formation ability,and bacterial adhesion to gastrointestinal mucosa.Results:The AKK-D strain has a probiotic function similar to that of the standard strain in humans(AKK-H).An in vivo study found that AKK-D significantly amelio-rated symptoms in the enterotoxigenic Escherichia coli(ETEC)-induced murine diar-rhea model.AKK-D improved organ damage,inhibited inflammatory responses,and improved intestinal barrier permeability.Additionally,AKK-D promoted the reconsti-tution and maintenance of the homeostasis of gut microflora,as indicated by the fact that AKK-D-treated mice showed a decrease in Bacteroidetes and an increase in the proportion of other beneficial bacteria like Muribaculaceae,Muribaculum,and unclas-sified f_Lachnospiaceae compared with the diarrhea model mice.Conclusion:Taken together,our data show that this novel AKK-D strain might be a potential probiotic for use in musk deer breeding,although further extensive system-atic research is still needed.
基金supported financially by the NSFC(Nos.62075098 and 62071119)the National Key Research and Development Program of China(Nos.2017YFA0205301 and 2018YFC1602905)。
文摘MicroRNAs(miRNAs)are abundant in the brain and mounting evidence suggests their involvement in the critical processes such as neurodevelopment,synaptic plasticity,and the development of neurodegenerative diseases.Thus,miRNAs may be promising therapeutic drugs for the treatment of neurodegenerative disorders.However,naked miRNAs are not able to enter cells directly,especially brain cells.Therefore,suitable carriers for safe and efficient miRNA delivery to brain cells are of great importance.Chitosan nanoparticles,with the excellent properties such as good compatibility and brilliant degradability,may act as a promising carrier for miRNA drug delivery.In this study,chitosan nanoparticles were prepared and their properties such as particle size,zeta potential and encapsulation efficiency were optimized to encapsulate miRNAs.The delivery efficiency of miRNA-loaded nanoparticles was then evaluated in both neuronal and microglia cells.The results demonstrated chitosan nanoparticles encapsulated miRNAs efficiently and showed excellent sustained releasing in vitro.Moreover,chitosan nanoparticles delivered miRNA to both neurons and microglia with very low toxicity and high efficiency.In conclusion,chitosan nanoparticles are promising carriers for the delivery of miRNAs to brain cells,which may be used for the early intervention and treatment of neurodegenerative disorders.
基金supported financially by the National Natural Science Foundation of China(NSFC,Nos.62071119 and 62075098)the National Key Research and Development Program of China(Nos.2017YFA0205301 and 2018YFC1602905)。
文摘The extracellular vesicles show great potential as a noninvasive biomarker for the early detection of cancer.Hence,there is an urgent requirement to create biosensors that are time-saving,simple,and easily scalable in order to accomplish rapid,sensitive,and quantitative detection of extracellular vesicles.In this study,we present a self-propelled DNA walker powered by endonuclease Nt.Bbv CI,which enables the development of a“signal on”sensing platform for the rapid and highly sensitive detection of extracellular vesicles.The DNA motor employed tracks made of streptavidin magnetic beads,which consisted of substrate strands labeled with fluorescein and motor strands locked by aptamers.The aptamer recognition of the target protein on extracellular vesicles unlocked the motor strand,initiating the DNA motor process.After replacing the optimal buffer solution containing the endonuclease Nt.BbvC I,the motor strands autonomously moved along the streptavidin magnetic beads track,continuously releasing fluorescent molecules and producing detectable fluorescence signals.Under optimal conditions,the detection range was from 2×10~4particles/mL to 2×10~9particles/mL,with a detection limit of 2.9×10~3particles/mL,demonstrating excellent selectivity.This method has demonstrated good selectivity in different tumorderived extracellular vesicles and performs well in complex biological samples.The ability to effectively analyze surface proteins of extracellular vesicles in a short period of time gives our DNA walker a tremendous potential for developing simple and cost-effective clinical diagnostic devices.
基金supported by grants from the Scientific Research Fund project of Education Department of Yunnan Province(2024J0314)Joint Special Project on Basic Research of Yunnan Provincial Department of Science and Technology and Kunming Medical University(202501AY070001-206).
文摘Objective:To evaluate the global liter-ature output on the relationship between brain-derived neurotrophic factor(BDNF)and cognitive function in al-cohol dependence syndrome using bibliometric methods and explore the status and trends in this field.Method:The literature on the application of BDNF in cognitive impairment caused by alcohol dependence syndrome published from 1995 to 2023 were retrieved from Web of Science,and the relevant information(publication characteristics,country and institution,author,number of publications,citation,journal and research field,corresponding author,key words,etc.)was recorded.The bibliometrix R package was used for quantitative and qualitative analysis of publication output and author contributions.Result:A total of 99 articles were included.The overall number of publications in this field has increased over time.The countries and institutions that contributed the most to the field were the United States and the Academy of Medical Sciences of Iranian universities,respectively.Most of the authors were from the United States,followed by Spain,China,and Iran.Ceccanti M,Fiore M were the most productive authors.Publications with Ceccanti M had the highest h-index.The most cited reference author is Haenninen H(227 citations),and the number one published journal is Alcohol.Most articles were published in 2020(n=12)and 2022(n=11),followed by 2019 and 2021(n=10).The corresponding author has the largest number of publications from the United States,and more publications from a single country tend to have more cooperation from other countries.BDNF and alcohol appeared more frequently in various keyword clouds.However,significant differences remained in the author keyword cloud,keyword plus word cloud,and paper topic word cloud.Conclusion:BDNF has great potential in the application of cognitive dysfunction caused by alcohol dependence syndrome.Bibliometric methods and data visualization techniques can help understand the current state of research progress and enable relevant scholars and practitioners to predict the development trends in this field.
基金financially supported by the NSF of China(No.61871180)2022 Special Project for the Construction of Innovative Provinces to Fight the COVID-19 Outbreak(No.2022SK2115)+1 种基金Natural Science Foundation of Hunan Province of China(Nos.2022JJ30230,2021JJ30213)the Deanship of Scientific Research,Taif University。
文摘A novel approach was developed to fabricate a label-free electrochemical aptasensor for specific detection of mercury ions(Hg^(2+)).This involved modifying polylysine(PLL)-coated black phosphorus-porous graphene(BP-PG)nanocomposites(PLL/BP-PG)onto the surface of glassy carbon electrodes(GCE),which were further modified with gold nanoparticles(AuNPs)to combine with a thiolated aptamer(Apt)capable of specifically recognizing Hg^(2+).BP-PG was synthesized using the solvothermal method and covalently bonded to form BP-PG nanosheets,resulting in significant enhanced electrochemical properties of the PLL/BP-PG composite.Furthermore,the PLL/BP-PG composite was improved environmental stability of BP and provided a considerable quantity of-NH_(2)for bonding to Au NPs firmly by assembling.The physical properties and electrochemical behavior of the substrate materials were investigated using various characterization techniques,and analytical parameters were optimized.It is shown that,the Apt/AuNPs/PLL/BP-PG/GCE had a linear response(R~2=0.999)with good selectivity and high sensitivity over the Hg^(2+)range of 1-10,000 nmol/L.The proposed sensor has a detection limit of 0.045 nmol/L and can be employed for detecting of Hg^(2+).It also obtained satisfying results in river water,soil and vegetable samples.
基金supported by the National Science Foundation of Sichuan Province(2023NSFSC1124)Fundamental Research Funds for the Central Universities(YJ2021141)the Science and Technology Cooperation Special Fund of Sichuan University and Zigong City(2022CDZG-9).
文摘High-voltage battery systems bring significant increases in energy density but are also accompanied by fast degradation of electrochemical performance and serious safety issues.Herein,Li^(+)coordination structure regulation was conducted to formulate a non-flammable electrolyte,which consists of 1.5 M lithium bis(fluor sulfonyl)imide(LiFSI)in triethyl phosphate and methyl 2,2,2-trifluoromethyl carbonate(FEMC).The renamed TEP-FEMC-FEC(TFF)electrolyte exhibits an FSI^(−)-dominated solvation structure contributed by the weakly-solvating ability of FEMC.The generated inorganic-rich interfacial layers are conducive to stabilizing the phase transition of high-voltage cathodes while suppressing the dendritic growth on lithium metal or co-intercalation behavior in graphite anode.This TFF electrolyte enables LiCoO_(2)||Li batteries to achieve capacity maintenance over 79%after 400 cycles with high-rate of 5 C at an ultra-high voltage of 4.6 V,and an outstanding capacity exceeding 100 mA h g^(−1)even at a super-high current density of 20 C.Additionally,the Ah-level LiCoO_(2)||graphite pouch cells also exhibit high capacity retention and satisfactory safety performance even under fast charging.This work provides a novel research direction for the pursuit of high energy density non-flammable electrolytes.
基金partially supported by the Open Project Program from the Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization(Ministry of Agriculture and Rural Affairs),China(212103)。
文摘Vegetable fields are often contaminated by heavy metals,and Spodoptera exigua is a major vegetable pest which is stressed by heavy metals mainly by feeding.In this study,cadmium accumulation in the tissues of S.exigua exposed to cadmium and its effects on the growth and development of the parents and the offspring were investigated.Under the stress of different concentrations of cadmium(0.2,3.2,and 51.2 mg kg^(-1)),the cadmium content in each tissue of S.exigua increased in a dose-dependent manner.At the larval stage,the highest cadmium accumulation was found in midgut in all three cadmium treatments,but at the adult stage,the highest cadmium content was found in fat body.In addition,the cadmium content in ovaries was much higher than in testes.When F1S.exigua was stressed by cadmium and the F_(2)generation was not fed a cadmium-containing diet,the larval survival,pupation rate,emergence rate and fecundity of the F_(2)generation were significantly reduced in the 51.2 mg kg^(-1)treatment compared to the corresponding F1generation.Even in the F_(2)generation of the 3.2 mg kg^(-1)treatment,the fecundity was significantly lower than in the parental generation.The fecundity of the only-female stressed treatment was significantly lower than that of the only-male stressed treatment at the 3.2 and 51.2 mg kg^(-1)cadmium exposure levels.When only mothers were stressed at the larval stage,the fecundity of the F_(2)generation was significantly lower than that of the F1generation in the 51.2 mg kg^(-1)treatment,and it was also significantly lower than in the 3.2 and 0.2 mg kg^(-1)treatments.The results of our study can provide useful information for forecasting the population increase trends under different heavy metal stress conditions and for the reliable environmental risk assessment of heavy metal pollution.
基金supported by the National Key Research and Development Program of China(Nos.2017YFA0205301 and 2018YFC1602905)National Natural Science Foundation of China(Nos.62071119,62075098,81902153,61527806)the Open Funding of State Key Laboratory of Oral Diseases(No.SKLOD2022OF05).
文摘Although it has been developed for many years, nucleic acid aptamer screening technology still fails to be widely used, a considerable part of it is due to the variability of tumor cell morphology, which leads to the use of immortalized cell lines in the laboratory to screen nucleic acid aptamers for recognition ability of tumor cells in the diseased body.To address this, primary cells that can be stably passaged were isolated and extracted from spontaneous tumors of genetically engineered pancreatic ductal adenocarcinoma model mice in this study.Next, an automated screening instrument for nucleic acid aptamers developed autonomously by our group was used to perform efficient aptamer screening using a limited number of cells, and the obtained nucleic acid aptamers were affinity verified at the cellular level.Finally, to answer the question of the cell growth environment difference on the recognition ability of nucleic acid aptamers, we verified its targeting ability to tumors in vivo on a nude mice xenograft tumor model, and further used a common antitumor drug doxorubicin combined with nucleic acid aptamers to verify the drug loading ability of this aptamer combined with the targeting therapeutic ability.
基金the NSFC(Nos.61701176 and 62071119)Macao FDCT(No.0065/2020/A2)+5 种基金Natural Science Foundation of Hunan Province of China(Nos.2022JJ50052,2018JJ3130 and 2020JJ5145)Hunan Key R&D Projects(No.2021SK2003)Nanjing Important Science&Technology Specific Projects(No.2021-11005)2022 Special Project for the Construction of Innovative Provinces to Fight the COVID-19 Outbreak(No.2022SK2115)Open Funding of State Key Laboratory of Oral Diseases(No.SKLOD2022OF05)Shenzhen Innovation and Entrepreneurship Program Innovation and Entrepreneurship Special Project(No.20220624181237005).
文摘The outbreak of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)in late 2019 has negatively affected people's lives and productivity.Because the mode of transmission of SARS-CoV-2 is of great concern,this review discusses the sources of virus aerosols and possible transmission routes.First,we discuss virus aerosol collection methods,including natural sedimentation,solid impact,liquid impact,centrifugal,cyclone and electrostatic adsorption methods.Then,we review common virus aerosol detection methods,including virus culture,metabolic detection,nucleic acid-based detection and immunology-based detection methods.Finally,possible solutions for the detection of SARS-CoV-2 aerosols are introduced.Point-of-care testing has long been a focus of attention.In the near future,the development of an instrument that integrates sampling and output results will enable the real-time,automatic monitoring of patients.
文摘Cytokinin is a critical growth regulator for various aspects of plant growth and development. In Arabidopsis, cytokinin signaling is mediated by a two-component system-based phosphorelay that transmits a signal from the receptors, through histidine phosphotransfer proteins, to the downstream response regulators （ARRs）. Of these ARRs, type-A ARR genes, whose transcription can be rapidly induced by cytokinin, act as negative regulators of eytokinin signaling. However, because of functional redundancy, the function of type-A ARR genes in plant growth and development is not well understood by analyzing loss-of-function mutants. In this study, we performed a comparative functional study on all ten type-A ARR genes by analyzing transgenic plants overexpressing these ARR genes fused to a MYC epitope tag. Overexpression of ARR genes results in a variety of cytokinin-associated phenotypes. Notably, overexpression of different ARR transgenes causes diverse phenotypes, even between phylogenetically closely-related gene pairs, such as within the ARR3-ARR4 and ARR5-ARR6 pairs. We found that the accumulation of a subset of ARR proteins （ARR3, ARR5, ARR7, ARR16 and ARR17; possibly ARR8 and ARR15） is increased by MG132, a specific proteasomal inhibitor, indicating that stability of these proteins is regulated by proteasomal degradation. Moreover, similar to that of previously characterized ARR5, ARR6 and ARR7, stability of ARR16 and ARR17, possibly including ARR8 and ARR15, is regulated by cytokinin. These results suggest that type-A ARR proteins are regulated by a combinatorial mechanism involving both the cytokinin and proteasome pathways, thereby executing distinctive functions in plant growth and development.
基金supported by NSFC (nos 61471168, 61571187,61301043,and 61527806)China Postdoctoral Science Foundation (2016T90403)the Economical Forest Cultivation and Utilization of 2011 Collaborative Innovation Center in Hunan Province [(2013)448]
文摘Tissue engineering has become a promising strategy for repairing damaged cartilage and bone tissue. Among the scaffolds for tissue-engineering applications, injectable hydrogels have demonstrated great potential for use as three-dimensional cell culture scaffolds in cartilage and bone tissue engineering, owing to their high water content, similarity to the natural extracellular matrix(ECM), porous framework for cell transplantation and proliferation, minimal invasive properties, and ability to match irregular defects. In this review, we describe the selection of appropriate biomaterials and fabrication methods to prepare novel injectable hydrogels for cartilage and bone tissue engineering. In addition, the biology of cartilage and the bony ECM is also summarized. Finally, future perspectives for injectable hydrogels in cartilage and bone tissue engineering are discussed.
