Bacteria of the genus Myroides （Myroides spp.） are rare opportunistic pathogens. Myroides sp. infections have been reported mainly in China. Myroides sp. is highly resistant to most available antibiotics, but the re...Bacteria of the genus Myroides （Myroides spp.） are rare opportunistic pathogens. Myroides sp. infections have been reported mainly in China. Myroides sp. is highly resistant to most available antibiotics, but the resistance mechanisms are not fully elucidated. Current strain identification methods based on biochemical traits are unable to identify strains accurately at the species level. While 16S ribosomal RNA （rRNA） gene sequencing can accurately achieve this, it fails to give information on the status and mechanisms of antibiotic resistance, because the 16S rRNA sequence contains no information on resistance genes, resistance islands or enzymes. We hypothesized that ob- taining the whole genome sequence of Myroides sp., using next generation sequencing methods, would help to clarify the mechanisms of pathogenesis and antibiotic resistance, and guide antibiotic selection to treat Myroides sp. infec- tions. As Myroides sp. can survive in hospitals and the environment, there is a risk of nosocomial infections and pandemics. For better management of Myroides sp. infections, it is imperative to apply next generation sequencing technologies to clarify the antibiotic resistance mechanisms in these bacteria.展开更多
To achieve ultrasonic casting of 35 CrMo steel,the waveguide unit for introducing ultrasound into liquid steel was studied numerically and experimentally.The structure and length of the ultrasonic waveguide were optim...To achieve ultrasonic casting of 35 CrMo steel,the waveguide unit for introducing ultrasound into liquid steel was studied numerically and experimentally.The structure and length of the ultrasonic waveguide were optimized by modal analysis.The simulation results showed that a T-shaped waveguide unit matched the vibrational system better than an L-shaped unit.The performance of T-shaped waveguide unit was optimized when the length of the ultrasound radiator was 135 mm.The performance of the T-shaped waveguide unit was investigated in ultrasonic casting experiments,and the effect of ultrasound on the microstructure of 35 CrMo steel was examined.The experimental results showed that the T-shaped waveguide was able to successfully introduce ultrasound into the 35 CrMo melt.In addition,the use of a silicon nitride ceramic radiator avoided high-temperature corrosion in the molten metal.The microstructure of the treated area was significantly refined and equiaxed grains were obtained.The results represented a novel method for ultrasonic casting of steel.展开更多
In an effort to understand the recombination of a B2 metallo-β-lactamase（MβL）,the binding of metals to apo-ImiS was studied by isothermal titration calorimetry and fluorescence spectra.The binding of Zn（Ⅱ）,Co...In an effort to understand the recombination of a B2 metallo-β-lactamase（MβL）,the binding of metals to apo-ImiS was studied by isothermal titration calorimetry and fluorescence spectra.The binding of Zn（Ⅱ）,Co（Ⅱ） to apo-lmiS resulted in activation free energies △G_≠~θ values of 93.719 and 92.948 kJ mol^（-1）,respectively,and increasing of fluorescence intensity at maxima emission of 340 nm.展开更多
The momentum space coupled channels optical（CCO） method for positron scattering has been extended to study the scattering of positrons by metastable helium for impact energies in the range from the positronium thres...The momentum space coupled channels optical（CCO） method for positron scattering has been extended to study the scattering of positrons by metastable helium for impact energies in the range from the positronium threshold up to high energies. Both the positronium formation and ionization continuum channels are included in the calculations via a complex equivalent local potential. The positronium formation, ionization, elastic and 2~3S–2~3P excitation, and total scattering cross sections are all presented and compared with the available information.展开更多
基金Project supported by the Huaqiao University Graduate Student Scientific Research Innovation Ability Cultivation Plan Projectsthe Major Program of Department of Science and Technology of Fujian Province(No.2012Y4009)+4 种基金the Science and Technology Planning Project of Xiamen(No.3502Z20123036)the Xiamen Southern Oceanographic Center(No.14GYY008NF08)the Construction Project for Yun Leung Laboratory for Molecular Diagnostics(No.14X30127)the Technology Planning Projects of Quanzhou Social Development Fields(No.2014Z24)the Major Support Research Project of National Key Colleges Construction of Quanzhou Medical College(No.2013A13),China
文摘Bacteria of the genus Myroides （Myroides spp.） are rare opportunistic pathogens. Myroides sp. infections have been reported mainly in China. Myroides sp. is highly resistant to most available antibiotics, but the resistance mechanisms are not fully elucidated. Current strain identification methods based on biochemical traits are unable to identify strains accurately at the species level. While 16S ribosomal RNA （rRNA） gene sequencing can accurately achieve this, it fails to give information on the status and mechanisms of antibiotic resistance, because the 16S rRNA sequence contains no information on resistance genes, resistance islands or enzymes. We hypothesized that ob- taining the whole genome sequence of Myroides sp., using next generation sequencing methods, would help to clarify the mechanisms of pathogenesis and antibiotic resistance, and guide antibiotic selection to treat Myroides sp. infec- tions. As Myroides sp. can survive in hospitals and the environment, there is a risk of nosocomial infections and pandemics. For better management of Myroides sp. infections, it is imperative to apply next generation sequencing technologies to clarify the antibiotic resistance mechanisms in these bacteria.
基金Item Sponsored by National Basic Research Program of China(2014CB046702)
文摘To achieve ultrasonic casting of 35 CrMo steel,the waveguide unit for introducing ultrasound into liquid steel was studied numerically and experimentally.The structure and length of the ultrasonic waveguide were optimized by modal analysis.The simulation results showed that a T-shaped waveguide unit matched the vibrational system better than an L-shaped unit.The performance of T-shaped waveguide unit was optimized when the length of the ultrasound radiator was 135 mm.The performance of the T-shaped waveguide unit was investigated in ultrasonic casting experiments,and the effect of ultrasound on the microstructure of 35 CrMo steel was examined.The experimental results showed that the T-shaped waveguide was able to successfully introduce ultrasound into the 35 CrMo melt.In addition,the use of a silicon nitride ceramic radiator avoided high-temperature corrosion in the molten metal.The microstructure of the treated area was significantly refined and equiaxed grains were obtained.The results represented a novel method for ultrasonic casting of steel.
基金supported by the National Natural Science Foundation of China (Nos.21272186 and 81361138018)
文摘In an effort to understand the recombination of a B2 metallo-β-lactamase（MβL）,the binding of metals to apo-ImiS was studied by isothermal titration calorimetry and fluorescence spectra.The binding of Zn（Ⅱ）,Co（Ⅱ） to apo-lmiS resulted in activation free energies △G_≠~θ values of 93.719 and 92.948 kJ mol^（-1）,respectively,and increasing of fluorescence intensity at maxima emission of 340 nm.
基金Project supported by the Natural Science Foundation of Heilongjiang Province of China(Grant No.12541160)
文摘The momentum space coupled channels optical（CCO） method for positron scattering has been extended to study the scattering of positrons by metastable helium for impact energies in the range from the positronium threshold up to high energies. Both the positronium formation and ionization continuum channels are included in the calculations via a complex equivalent local potential. The positronium formation, ionization, elastic and 2~3S–2~3P excitation, and total scattering cross sections are all presented and compared with the available information.
