Exopolysaccharides(EPS)derived from lactic acid bacteria have received considerable attention because of their diverse bioactivities and high safety.In our previous work,a crude EPS produced by Lactiplantibacillus pla...Exopolysaccharides(EPS)derived from lactic acid bacteria have received considerable attention because of their diverse bioactivities and high safety.In our previous work,a crude EPS produced by Lactiplantibacillus plantarum Z-1 under H2O2 stress and its three purified fractions were prepared and characterized.In the present work,antioxidant and anti-inflammatory activities of these EPSs were investigated.The four EPSs were found to possess considerable free radical scavenging activity,and be able to decrease the ROS and MDA levels in H_(2)O_(2)-induced oxidative stress RAW 264.7 macrophages,and increase the activities of SOD and CAT enzymes.It is likely that activating Nrf2/HO-1 signaling pathway is one of their important antioxidant mechanisms.These EPSs were also found to effectively reduce the secretion of inflammatory mediators,such as pro-inflammatory factors(TNF-α,IL-1β,IL6)and NO,and increase the release of anti-inflammatory factor IL-10.The anti-inflammatory effect of EPSs could be due to their ability to inhibit the activation of NF-κB/TLR4 signaling pathway and intracellular COX-2 signaling cascade.Therefore,these EPS fractions have good antioxidant and antiinflammatory activities,providing useful enlightenment for the application of these EPSs in functional foods and medicines.展开更多
基金supported by“Pioneer”and“Leading Goose”R&D Program of Zhejiang of China(2022C02012).
文摘Exopolysaccharides(EPS)derived from lactic acid bacteria have received considerable attention because of their diverse bioactivities and high safety.In our previous work,a crude EPS produced by Lactiplantibacillus plantarum Z-1 under H2O2 stress and its three purified fractions were prepared and characterized.In the present work,antioxidant and anti-inflammatory activities of these EPSs were investigated.The four EPSs were found to possess considerable free radical scavenging activity,and be able to decrease the ROS and MDA levels in H_(2)O_(2)-induced oxidative stress RAW 264.7 macrophages,and increase the activities of SOD and CAT enzymes.It is likely that activating Nrf2/HO-1 signaling pathway is one of their important antioxidant mechanisms.These EPSs were also found to effectively reduce the secretion of inflammatory mediators,such as pro-inflammatory factors(TNF-α,IL-1β,IL6)and NO,and increase the release of anti-inflammatory factor IL-10.The anti-inflammatory effect of EPSs could be due to their ability to inhibit the activation of NF-κB/TLR4 signaling pathway and intracellular COX-2 signaling cascade.Therefore,these EPS fractions have good antioxidant and antiinflammatory activities,providing useful enlightenment for the application of these EPSs in functional foods and medicines.
