Water channel proteins facilitate water flux across cell membranes and play important roles in plant growth and development. By GUS histochemical assay in RWC3 promoter-GUS transgenic rice (Oryza sativa L. cv. Shenxia...Water channel proteins facilitate water flux across cell membranes and play important roles in plant growth and development. By GUS histochemical assay in RWC3 promoter-GUS transgenic rice (Oryza sativa L. cv. Shenxiangjin 4), one of the members of water channel proteins in rice, RWC3, was found to distribute widely in variety of organs, from vegetative and reproductive organs. Further studies showed that gibberellin (GA) enhanced the GUS activity in the transgenic calli, suspension cells and leaves, whereas ancymidol (anc), an inhibitor of GA synthesis, reduced the GUS activity. Sucrose was found to inhibit the effects induced by addition of GA, suggesting a possible cross-talk between GA and sucrose signaling on regulation of the RWC3 gene expression.展开更多
Bacterial growth potential(BGP) method and two parallel pilot scale biofilters were used to investigate phosphorus limitation and its effect on the removal of organic matters in biofiltration for drinking water treat...Bacterial growth potential(BGP) method and two parallel pilot scale biofilters were used to investigate phosphorus limitation and its effect on the removal of organic matters in biofiltration for drinking water treatment. Addition of phosphorus can substantially increase the BGPs of the samples. Its effect was equivalent to that of addition of a mixture of various inorganic nutrients including phosphorus. The biofilter with phosphate added into its influent performed a higher biological stability of the effluent and a higher COD Mn removal than the control filter. These results suggested that phosphorus was the limiting nutrient in the biofiltration and the removal efficiency of organic matters could be improved by adding phosphate into the influent.展开更多
Good class management is the prerequisite for all normal class work. The first grade is a critical period for cultivating high-quality class teams. The first-year students are in the transition period of their physica...Good class management is the prerequisite for all normal class work. The first grade is a critical period for cultivating high-quality class teams. The first-year students are in the transition period of their physical and psychological development, and the reasonable management of the class teacher is very important. From the formation of good habits to internalization as a quality of oneself and long-term fixation, from "heteronomy" to passively manage oneself to "self-discipline" to form autonomous management habits and then transform into ability. The self-managed and well-managed class collective not only has a positive class culture, but also enables students to grow in the continuous innovation and improvement of the class culture in order to adapt to the actual educational environment.展开更多
Background MicroRNAs （miRNAs） control gene expression by destabilizing target transcripts and inhibiting their translation. Aberrant expression of miRNAs has been described in many human diseases, including schizoph...Background MicroRNAs （miRNAs） control gene expression by destabilizing target transcripts and inhibiting their translation. Aberrant expression of miRNAs has been described in many human diseases, including schizophrenia. However, the effects on miRNA expression in response to antipsychotic treatment in peripheral circulation have not been thoroughly examined. Methods Using quantitative real-time PCR （qRT-PCR）, We quantified the expression of seven candidate miRNAs in plasma samples of 40 first-episode schizophrenics before and after antipsychotic treatment. The patients were all treated with risperidone and achieved remission in 1 year. Results Compared with the baseline, the expression levels of miR-365 and miR-520c-3p were significantly down- regulated after 1 year of risperidone treatment （P 〈0.001）. There were no significant correlations between the clinical symptoms and the expression levels of these two miRNAs （P 〉0.05）. Conclusions This study analyzed possible circulating miRNAs in response to antipsychotic monotherapy for schizophrenia, the further mechanism need to be confirmed.展开更多
All organisms with oxygenic photosynthesis contain two photosystems: photosystemⅠ(PSⅠ) and photo-system Ⅱ (PSⅡ). The minimal photosystem Ⅱ particles which are photochemically active contain three subunits: D1, D2...All organisms with oxygenic photosynthesis contain two photosystems: photosystemⅠ(PSⅠ) and photo-system Ⅱ (PSⅡ). The minimal photosystem Ⅱ particles which are photochemically active contain three subunits: D1, D2 and cytochrome b559 (Cyt b559). The function of Cyt b559 remains unclear. We have successfully overexpressed the psbF gene, encoding the b subunit of Cyt b559, from a marine cyanobacterium Synechococcus sp. PCC 7002 as a fusion gene and obtained a redox-active form of Cyt b559. When the N-terminal GST protein of the fusion gene product was removed with thrombin, the PsbF protein was still re-dox-active, suggesting that the recombinant PsbF can form dimer in Escherichia coli. The absorption spectra of either the oxidized form or the reduced form of both GST fusion protein and the purified PsbF dimer and the difference spec-tra between the two forms are the same as that of the Cyt b559 isolated from the higher plants. Redox titration analysis of recombinant PsbF showed that the mid-point redox potential of the recombinant Cyt b559 was approximately 50 mV, which is close to the low potential of Cyt b559. The results are helpful to the understanding of localization and function of Cyt b559 on thylakoid membranes.展开更多
文摘Water channel proteins facilitate water flux across cell membranes and play important roles in plant growth and development. By GUS histochemical assay in RWC3 promoter-GUS transgenic rice (Oryza sativa L. cv. Shenxiangjin 4), one of the members of water channel proteins in rice, RWC3, was found to distribute widely in variety of organs, from vegetative and reproductive organs. Further studies showed that gibberellin (GA) enhanced the GUS activity in the transgenic calli, suspension cells and leaves, whereas ancymidol (anc), an inhibitor of GA synthesis, reduced the GUS activity. Sucrose was found to inhibit the effects induced by addition of GA, suggesting a possible cross-talk between GA and sucrose signaling on regulation of the RWC3 gene expression.
文摘Bacterial growth potential(BGP) method and two parallel pilot scale biofilters were used to investigate phosphorus limitation and its effect on the removal of organic matters in biofiltration for drinking water treatment. Addition of phosphorus can substantially increase the BGPs of the samples. Its effect was equivalent to that of addition of a mixture of various inorganic nutrients including phosphorus. The biofilter with phosphate added into its influent performed a higher biological stability of the effluent and a higher COD Mn removal than the control filter. These results suggested that phosphorus was the limiting nutrient in the biofiltration and the removal efficiency of organic matters could be improved by adding phosphate into the influent.
文摘Good class management is the prerequisite for all normal class work. The first grade is a critical period for cultivating high-quality class teams. The first-year students are in the transition period of their physical and psychological development, and the reasonable management of the class teacher is very important. From the formation of good habits to internalization as a quality of oneself and long-term fixation, from "heteronomy" to passively manage oneself to "self-discipline" to form autonomous management habits and then transform into ability. The self-managed and well-managed class collective not only has a positive class culture, but also enables students to grow in the continuous innovation and improvement of the class culture in order to adapt to the actual educational environment.
文摘Background MicroRNAs （miRNAs） control gene expression by destabilizing target transcripts and inhibiting their translation. Aberrant expression of miRNAs has been described in many human diseases, including schizophrenia. However, the effects on miRNA expression in response to antipsychotic treatment in peripheral circulation have not been thoroughly examined. Methods Using quantitative real-time PCR （qRT-PCR）, We quantified the expression of seven candidate miRNAs in plasma samples of 40 first-episode schizophrenics before and after antipsychotic treatment. The patients were all treated with risperidone and achieved remission in 1 year. Results Compared with the baseline, the expression levels of miR-365 and miR-520c-3p were significantly down- regulated after 1 year of risperidone treatment （P 〈0.001）. There were no significant correlations between the clinical symptoms and the expression levels of these two miRNAs （P 〉0.05）. Conclusions This study analyzed possible circulating miRNAs in response to antipsychotic monotherapy for schizophrenia, the further mechanism need to be confirmed.
基金supported by the State"973"Key Basic Research Project(Grant No.G1998010100)the National Natural Science Foundation of China(Grant No.39535002).
文摘All organisms with oxygenic photosynthesis contain two photosystems: photosystemⅠ(PSⅠ) and photo-system Ⅱ (PSⅡ). The minimal photosystem Ⅱ particles which are photochemically active contain three subunits: D1, D2 and cytochrome b559 (Cyt b559). The function of Cyt b559 remains unclear. We have successfully overexpressed the psbF gene, encoding the b subunit of Cyt b559, from a marine cyanobacterium Synechococcus sp. PCC 7002 as a fusion gene and obtained a redox-active form of Cyt b559. When the N-terminal GST protein of the fusion gene product was removed with thrombin, the PsbF protein was still re-dox-active, suggesting that the recombinant PsbF can form dimer in Escherichia coli. The absorption spectra of either the oxidized form or the reduced form of both GST fusion protein and the purified PsbF dimer and the difference spec-tra between the two forms are the same as that of the Cyt b559 isolated from the higher plants. Redox titration analysis of recombinant PsbF showed that the mid-point redox potential of the recombinant Cyt b559 was approximately 50 mV, which is close to the low potential of Cyt b559. The results are helpful to the understanding of localization and function of Cyt b559 on thylakoid membranes.
