Lipocalin-2(LCN2)is a member of the lipocalin superfamily with multiple functions and can participate in the transport of a variety of small lipophilic ligands in vivo.LCN2 is significantly expressed in various tumors...Lipocalin-2(LCN2)is a member of the lipocalin superfamily with multiple functions and can participate in the transport of a variety of small lipophilic ligands in vivo.LCN2 is significantly expressed in various tumors and plays an important role in regulating tumor cell proliferation,invasion,and metastasis.The specific actions of LCN2 in tumors may vary depending on the particular type of cancer involved.In this review,we provide an extensive overview of the transcriptional and post-transcriptional regulation of LCN2 in health and disease.Furthermore,we summarize the impact of LCN2 dysregulation in a broad range of tumors.Lastly,we examine the mechanisms of action of LCN2 during tumorigenesis,progression,and metastasis.Understanding the complex relationships between LCN2 and tumor development,progression,and metastasis is vital for advancing our knowledge of cancer biology,developing biomarkers for diagnosis and clinical decision-making,and creating therapeutic strategies to improve the management of patients with cancer.展开更多
Agriculture is the basic industry that concerns the national economy and people’s livelihood. In the process of transforming to modern agriculture, the traditional agriculture in our country faces the problems of ens...Agriculture is the basic industry that concerns the national economy and people’s livelihood. In the process of transforming to modern agriculture, the traditional agriculture in our country faces the problems of ensuring the quality of agricultural production, adjusting agricultural industrial structures, improving the low production efficiency and low utilization rate of resources, and environmental pollution, thus it cannot meet the needs of sustainable agricultural development. Therefore, the research on intelligent agriculture technology is imperative. This paper analyzes the key technologies of Internet of things applied in the intelligent agriculture, presents the application of Internet of things technology in agricultural planting system, constructs the intelligent agricultural planting system based on the Internet of things technology, and designs the framework of the management platform.展开更多
The prevention and treatment of pathological scars remain challenging.Corticosteroids are the mainstay drugs in clinical scar prevention and treatment as they effectively induce scar regression and improve scar prurit...The prevention and treatment of pathological scars remain challenging.Corticosteroids are the mainstay drugs in clinical scar prevention and treatment as they effectively induce scar regression and improve scar pruritus and pain.Currently,intralesional injections of corticosteroids are widely used in clinical practice.These require professional medical manipulation;however,the significant accompanying injection pain,repetition of injections and adverse effects,such as skin atrophy,skin pigmentation and telangiectasia,make this treatment modality an unpleasant experience for patients.Transdermal administration is,therefore,a promising non-invasive and easy-to-use method for corticosteroid administration for scar treatment.In this review,we first summarize the mechanisms of action of corticosteroids in scar prevention and treatment;then,we discuss current developments in intralesional injections and the progress of transdermal delivery systems of corticosteroids,as well as their corresponding advantages and disadvantages.展开更多
Background:Hypertrophic scars(HS)represent one of the most common clinical challenges due to unsatisfactory therapeutic results.HS formation is associated with the abnormal activation of fibroblasts and their excessiv...Background:Hypertrophic scars(HS)represent one of the most common clinical challenges due to unsatisfactory therapeutic results.HS formation is associated with the abnormal activation of fibroblasts and their excessive fibrotic behavior.Glycolysis dysregulation has been shown to participate in the incidence and progression of various fibrotic diseases and shows potential as a means of controlling HS formation.This work aimed to discuss the impact of augmented glycolysis on HS and to propose a method for controlling HS formation through glycolysis regulation.Methods:Here,augmented glycolysis was confirmed together with enhanced fibrotic activity in both HS fibroblasts(HFs)and HS tissues,and the suppression of glycolysis also attenuated fibroblast activation.We also introduced IR780,a heptamethine cyanine dye,to regulate glycolysis for the control of HS formation.Results:In vitro,cell studies indicated that IR780 significantly down-regulated glycolysis and suppressed the fibrotic activity of HFs.In vivo,the intralesional injection of IR780 into rabbit HS models led to the downregulation of glycolysis and the control of HS formation.Furthermore,IR780 accumulated preferentially in activated fibroblasts in both in vitro and in vivo studies,and thus specifically downregulated glycolysis and efficiently controlled fibrosis by targeting activated fibroblasts.Conclusions:This work identified a strategy for controlling fibrosis and HS formation from the perspective of glycolysis regulation with IR780 targeting of activated fibroblasts.展开更多
Gastrointestinal infection is a leading cause of gut diseases attracting global health concerns.The emerging antimicrobial resistance in enteric pathogens drives the search of viable and renewable alternatives to anti...Gastrointestinal infection is a leading cause of gut diseases attracting global health concerns.The emerging antimicrobial resistance in enteric pathogens drives the search of viable and renewable alternatives to antibiotics for the health of both human beings and animals.Spore-forming probiotic Bacillus have received extensively interests for their multiple health benefits,including the restoration of microbiota dysbiosis and the reduction of drug-resistant pathogens.These promising benefits are mainly attributed to the activity of structurally diverse Bacillus-derived metabolites,such as antibacterial compounds,short-chain fatty acids,and other small molecules.Such metabolites show the capacity to directly target either the individual or community of bacterial pathogens,and to potentiate both host cells and gut microbiota.The better understanding of the mechanisms by which probiotic Bacillus and the metabolites modulate the metabolism of hosts and microbiota will advance the screening and development of probiotic Bacillus.In this review,we discuss the interaction among probiotic Bacillus,microbiota and host,and summarize the Bacillus-derived metabolites that act as key players in such interactions,shedding light on the mechanistic understanding of probiotic Bacillus against enteric bacterial infections.展开更多
Non-spherical Gold Nanoparticles(AuNPs),with unique geometries properties,have become the new exciting focus of applications in tumor imaging and therapy recently.In this review,we summarized the properties and applic...Non-spherical Gold Nanoparticles(AuNPs),with unique geometries properties,have become the new exciting focus of applications in tumor imaging and therapy recently.In this review,we summarized the properties and applications of non-spherical AuNPs for cancer imaging and therapy.We review four typical shapes of non-spherical AuNPs from their applications and advantages with the great optimism to the applications of non-spherical AuNPs in medicine.展开更多
Machine learning techniques are widely used for the diagnosis of cancers.In this study,we proposed a classification and prediction system for the diagnosis of gastric cancer based on saliva samples.Gastric cancer(GC)i...Machine learning techniques are widely used for the diagnosis of cancers.In this study,we proposed a classification and prediction system for the diagnosis of gastric cancer based on saliva samples.Gastric cancer(GC)is classified into early gastric cancer(EGC)and advanced gastric cancer(AGC).The diagnosis of GC at an early stage will improve the survival rate.Computer-aided diagnostic(CAD)systems can assist the radiologists in the diagnosis of EGC.220 saliva samples were collected from the non-cancerous and gastric cancerous persons and analyzed using high-performance liquid chromatography-mass spectrometry(HPLC-MS).Fourteen amino acid biomarkers were sufficient to distinguish the persons from malignant to benign and were observed in the saliva samples with dominant peaks.We used the support vector machine(SVM)for binary classification.The processed Raman dataset was used to train and test the developed model.SVM based neural networks were established using different kernels,which produced different results.Accuracy,specificity,sensitivity,and receiver operating characteristics(ROC)were used to evaluate the proposed classification model,along with mean average error(MAE),mean square Error(MSE),sum average error(SAE),and sum square error(SSE).We achieved an overall accuracy of 97.18%,specificity of 97.44%,and sensitivity of 96.88%for the proposed method.This established method owns the prospect of clinical translation.展开更多
Acne is a common chronic inflammatory dermatosis that can lead to pathological scars(PSs,divided into hypertrophic scars and keloids).These kinds of abnormal scars seriously reduce the quality of life of patients.Howe...Acne is a common chronic inflammatory dermatosis that can lead to pathological scars(PSs,divided into hypertrophic scars and keloids).These kinds of abnormal scars seriously reduce the quality of life of patients.However,their mechanism is still unclear,resulting in difficult clinical prevention,unstable treatment effects and a high risk of recurrence.Available evidence supports inflammatory changes caused by infection as one of the keys to abnormal proliferation of skin fibroblasts.In acne-induced PSs,increasing knowledge of the immunopathology indicates that inflammatory cells directly secrete growth factors to activate fibroblasts and release proinflammatory factors to promote the formation of PSs.T helper cells contribute to PSs via the secretion of interleukin(IL)-4 and IL-13,the pro-inflammatory factors;while regulatory T cells have anti-inflammatory effects,secrete IL-10 and prostaglandin E2,and suppress fibrosis production.Several treatments are available,but there is a lack of combination regimens to target different aspects of acne-induced PSs.Overall,this review indicates that the joint involvement of inflammatory response and fibrosis plays a crucial role in acne-induced PSs,and also analyzes the interaction of current treatments for acne and PS.展开更多
A unique,sensitive,and highly specific fluoroimmunoassay system for antigen detection using quantum dot and gold nanoparticles has been developed.The assay is based on the fluorescence quenching of quantum dots caused...A unique,sensitive,and highly specific fluoroimmunoassay system for antigen detection using quantum dot and gold nanoparticles has been developed.The assay is based on the fluorescence quenching of quantum dots caused by gold nanoparticles coated with antibody.To demonstrate its analytical capabilities,the quantum dots were coated with anti-HBsAg monoclonal antibodies(QDs-MAb1)and gold nanoparticles coated with another anti-HBsAg monoclonal antibodies(GNPs-MAb2)which specifically bound with HBsAg could sandwich the HBsAg captured by the immunoreactions.The sandwich-type immunocomplex was formed and the energy of quantum dots was transferred to gold nanoparticles as they were within a short distance,so that the fluorescence intensity of quantum dots was quenched.The fluorescence intensity of quantum dots at 570 nm was negative linear proportional to HBsAg concentration logarithm.The result showed that the limit of detection of the HBsAg was 0.928 ng/mL.This new system can be extended to detect target molecules with matched antibodies and has broad potential applications in immunoassay and disease diagnosis.展开更多
Photodynamic therapy(PDT)is an emerging therapeutic strategy for hypertrophic scars(HS),which is heavily dependent on reactive oxygen species(ROS)generation.However,the unsatisfactory delivery and excitation of 5-amin...Photodynamic therapy(PDT)is an emerging therapeutic strategy for hypertrophic scars(HS),which is heavily dependent on reactive oxygen species(ROS)generation.However,the unsatisfactory delivery and excitation of 5-aminolevulinic acid(ALA,a commercial photosensitizer in dermatology)result in an insufficient ROS generation,and thus limit the clinical application of PDT treating HS(HS-PDT).Consequently,sophisticated transdermal co-delivery nanoethosomes(named A/A-ES)with ALA and Au nanotriangles(AuNTs)in cores are prepared via an in-situ seed-mediated growth method,and then applied to improve HS-PDT through localized surface plasmon resonance(LSPR)-enhanced ROS generation.A/A-ES display a satisfactory performance in co-delivery in HS tissue with sufficient protoporphyrin IX production and LSPR effect in cytoplasm,which is beneficial for ALA excitation as well as ROS generation.In vitrolvivo studies reveal that A/A-ES significantly improve HS-PDT in promoting to fibroblast apoptosis and collagen remodeling through LSPR-enhanced ROS generation.Therefore,this study provides a feasible strategy that integrates transdermal delivery and LSPR to enable the beneficial effects of HS-PDT through boosting the delivery and excitation of ALA.展开更多
Importance: The current lack of reliable rapid tests for distinguishing between bacterial and viral infections has contributed to antibiotic misuse.Objective: This study aimed to develop a novel biomarker assay that i...Importance: The current lack of reliable rapid tests for distinguishing between bacterial and viral infections has contributed to antibiotic misuse.Objective: This study aimed to develop a novel biomarker assay that integratesFAM89A andIFI44L measurements to assist in differentiating between bacterial and viral infections.Methods: This prospective study recruited children with febrile illness from two hospitals between July 1, 2018, and June 30, 2019. A panel of three experienced pediatricians performed reference standard diagnoses of all patients (i.e., bacterial or viral infection) using available clinical and laboratory data, including a 28-day follow-up assessment. Assay operators were blinded to the reference standard diagnoses. The expression levels ofFAM89A andIFI44L were determined by quantitative real-time polymerase chain reaction assessment.Results: Of 133 potentially eligible patients with suspected bacterial or viral infection, 35 were excluded after the application of exclusion criteria. The resulting cohort included 98 patients: 59 with viral diagnoses and 39 with bacterial diagnoses. The areas under the curve (AUCs) of diagnoses usingFAM89A andIFI44L were 0.694 [95% confidence interval (CI): 0.583-0.804] and 0.751 (95%CI: 0.651-0.851), respectively. The disease risk score (DRS) [log2(FAM89A expression) - log2(IFI44L expression)] signature achieved an improved area under the receiver operating characteristic curve (AUC, 0.825;95%CI: 0.735-0.915), compared with the AUC generated from individual host RNA. A combination of the DRS and the C-reactive protein (CRP) level achieved an AUC of 0.896 (95%CI: 0.825-0.966). Optimal cutoffs for the DRS and CRP level were -3.18 and 19.80 mg/L, respectively.Interpretation: The DRS was significantly more accurate than the CRP level in distinguishing between bacterial and viral infections;the combination of these two parameters exhibited greater sensitivity and specificity. This study provides information that could be useful for the clinical application ofFAM89A andIFI44L in terms of distinguishing between viral and bacterial infections.展开更多
The novel coronavirus pneumonia,a global pandemic disease named as coronavirus disease 2019,has caused enormous losses on the health and economies of people all over the world,while there is still a lack of quick and ...The novel coronavirus pneumonia,a global pandemic disease named as coronavirus disease 2019,has caused enormous losses on the health and economies of people all over the world,while there is still a lack of quick and sensitive diagnostic method and effective therapy.Developing rapid diagnostic method for coronavirus disease 2019 has become exceptional urgent.Herein we report a rapid diagnostic method for the novel coronavirus through monitoring the volatile biomarkers in human exhaled breath.The breath volatile biomarkers are derived from the metabolism of novel coronavirus,including acetoin,2,4,6-trimethylpyridine,3-methyl tridecane,tetradecane,isooctyl alcohol,pentadecane,hexadecane,1-methylene-1H-indene.By comparing the types and concentrations of the volatile biomarkers in human exhaled breath combined with SERS sensor,we could distinguish between the healthy person and the patients with coronavirus disease 2019.This work confirms that various volatile organic compounds metabolized by novel coronavirus can be employed for rapidly screening of patients with coronavirus disease 2019,and has broad application prospects in the prevention and control of the epidemic.展开更多
基金supported by the Program for Science Technology and Innovation Committee of Shenzhen(2021N062-JCYJ20210324115408023)Guangdong High-Level Hospital Construction Fund,Shenzhen High-Level Hospital Construction Fund。
文摘Lipocalin-2(LCN2)is a member of the lipocalin superfamily with multiple functions and can participate in the transport of a variety of small lipophilic ligands in vivo.LCN2 is significantly expressed in various tumors and plays an important role in regulating tumor cell proliferation,invasion,and metastasis.The specific actions of LCN2 in tumors may vary depending on the particular type of cancer involved.In this review,we provide an extensive overview of the transcriptional and post-transcriptional regulation of LCN2 in health and disease.Furthermore,we summarize the impact of LCN2 dysregulation in a broad range of tumors.Lastly,we examine the mechanisms of action of LCN2 during tumorigenesis,progression,and metastasis.Understanding the complex relationships between LCN2 and tumor development,progression,and metastasis is vital for advancing our knowledge of cancer biology,developing biomarkers for diagnosis and clinical decision-making,and creating therapeutic strategies to improve the management of patients with cancer.
文摘Agriculture is the basic industry that concerns the national economy and people’s livelihood. In the process of transforming to modern agriculture, the traditional agriculture in our country faces the problems of ensuring the quality of agricultural production, adjusting agricultural industrial structures, improving the low production efficiency and low utilization rate of resources, and environmental pollution, thus it cannot meet the needs of sustainable agricultural development. Therefore, the research on intelligent agriculture technology is imperative. This paper analyzes the key technologies of Internet of things applied in the intelligent agriculture, presents the application of Internet of things technology in agricultural planting system, constructs the intelligent agricultural planting system based on the Internet of things technology, and designs the framework of the management platform.
基金supported by the National Natural Science Foundation of China(grant numbers:82172222,82272266 and 82102328)the Shanghai Clinical Research Project of Health Industry(20204Y0443)+1 种基金the Cross Research Project of Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine(JYJC202009)the Shanghai Clinical Research Center of Plastic and Reconstructive Surgery supported by Science and Technology Commission of Shanghai Municipality(Grant No.22MC1940300).
文摘The prevention and treatment of pathological scars remain challenging.Corticosteroids are the mainstay drugs in clinical scar prevention and treatment as they effectively induce scar regression and improve scar pruritus and pain.Currently,intralesional injections of corticosteroids are widely used in clinical practice.These require professional medical manipulation;however,the significant accompanying injection pain,repetition of injections and adverse effects,such as skin atrophy,skin pigmentation and telangiectasia,make this treatment modality an unpleasant experience for patients.Transdermal administration is,therefore,a promising non-invasive and easy-to-use method for corticosteroid administration for scar treatment.In this review,we first summarize the mechanisms of action of corticosteroids in scar prevention and treatment;then,we discuss current developments in intralesional injections and the progress of transdermal delivery systems of corticosteroids,as well as their corresponding advantages and disadvantages.
基金supported by National Natural Science Foundation of China(82172222 and 82102328)ShanghaiMunicipal Education Commission-Gaofeng Clinical Medicine Grant Support(20152227)+3 种基金ClinicalMulti-Disciplinary Team Research Program of ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine(2017-1-007)Cross Research Project of Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine(JYJC202009)Shanghai Health Industry Clinical Research Special Project(20204Y0443)Shanghai Municipal Key Clinical Specialty(shslczdzk00901).
文摘Background:Hypertrophic scars(HS)represent one of the most common clinical challenges due to unsatisfactory therapeutic results.HS formation is associated with the abnormal activation of fibroblasts and their excessive fibrotic behavior.Glycolysis dysregulation has been shown to participate in the incidence and progression of various fibrotic diseases and shows potential as a means of controlling HS formation.This work aimed to discuss the impact of augmented glycolysis on HS and to propose a method for controlling HS formation through glycolysis regulation.Methods:Here,augmented glycolysis was confirmed together with enhanced fibrotic activity in both HS fibroblasts(HFs)and HS tissues,and the suppression of glycolysis also attenuated fibroblast activation.We also introduced IR780,a heptamethine cyanine dye,to regulate glycolysis for the control of HS formation.Results:In vitro,cell studies indicated that IR780 significantly down-regulated glycolysis and suppressed the fibrotic activity of HFs.In vivo,the intralesional injection of IR780 into rabbit HS models led to the downregulation of glycolysis and the control of HS formation.Furthermore,IR780 accumulated preferentially in activated fibroblasts in both in vitro and in vivo studies,and thus specifically downregulated glycolysis and efficiently controlled fibrosis by targeting activated fibroblasts.Conclusions:This work identified a strategy for controlling fibrosis and HS formation from the perspective of glycolysis regulation with IR780 targeting of activated fibroblasts.
基金supported by the National Key Research and Development Program of China(2022YFD1801600).
文摘Gastrointestinal infection is a leading cause of gut diseases attracting global health concerns.The emerging antimicrobial resistance in enteric pathogens drives the search of viable and renewable alternatives to antibiotics for the health of both human beings and animals.Spore-forming probiotic Bacillus have received extensively interests for their multiple health benefits,including the restoration of microbiota dysbiosis and the reduction of drug-resistant pathogens.These promising benefits are mainly attributed to the activity of structurally diverse Bacillus-derived metabolites,such as antibacterial compounds,short-chain fatty acids,and other small molecules.Such metabolites show the capacity to directly target either the individual or community of bacterial pathogens,and to potentiate both host cells and gut microbiota.The better understanding of the mechanisms by which probiotic Bacillus and the metabolites modulate the metabolism of hosts and microbiota will advance the screening and development of probiotic Bacillus.In this review,we discuss the interaction among probiotic Bacillus,microbiota and host,and summarize the Bacillus-derived metabolites that act as key players in such interactions,shedding light on the mechanistic understanding of probiotic Bacillus against enteric bacterial infections.
基金supported by the National Key Basic Research Program(973 Project)(No.2011CB933100)National Natural Scientific Fund(No.81225010,81327002,and 31100717)+2 种基金863 project of China(2012AA022703)Shanghai Science and Technology Fund(No.13NM1401500 and 11nm0504200)Shanghai Jiao Tong University Innovation Fund for Postgraduates(No.AE340011).
文摘Non-spherical Gold Nanoparticles(AuNPs),with unique geometries properties,have become the new exciting focus of applications in tumor imaging and therapy recently.In this review,we summarized the properties and applications of non-spherical AuNPs for cancer imaging and therapy.We review four typical shapes of non-spherical AuNPs from their applications and advantages with the great optimism to the applications of non-spherical AuNPs in medicine.
基金supported by 973 Project(2017FYA 0205304)National Natural Science Foundation of China(No.81225010,81028009 and 31170961)the Research Fund of Yantai Information Technology Research Institute of Shanghai Jiao Tong University.
文摘Machine learning techniques are widely used for the diagnosis of cancers.In this study,we proposed a classification and prediction system for the diagnosis of gastric cancer based on saliva samples.Gastric cancer(GC)is classified into early gastric cancer(EGC)and advanced gastric cancer(AGC).The diagnosis of GC at an early stage will improve the survival rate.Computer-aided diagnostic(CAD)systems can assist the radiologists in the diagnosis of EGC.220 saliva samples were collected from the non-cancerous and gastric cancerous persons and analyzed using high-performance liquid chromatography-mass spectrometry(HPLC-MS).Fourteen amino acid biomarkers were sufficient to distinguish the persons from malignant to benign and were observed in the saliva samples with dominant peaks.We used the support vector machine(SVM)for binary classification.The processed Raman dataset was used to train and test the developed model.SVM based neural networks were established using different kernels,which produced different results.Accuracy,specificity,sensitivity,and receiver operating characteristics(ROC)were used to evaluate the proposed classification model,along with mean average error(MAE),mean square Error(MSE),sum average error(SAE),and sum square error(SSE).We achieved an overall accuracy of 97.18%,specificity of 97.44%,and sensitivity of 96.88%for the proposed method.This established method owns the prospect of clinical translation.
基金supported by the National Natural Science Foundation of China(grant numbers:82172222,82272266 and 82102328)the Shanghai Clinical Research Project of the Health Industry(20204Y0443)the Cross Research Project of the Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine(JYJC202009).
文摘Acne is a common chronic inflammatory dermatosis that can lead to pathological scars(PSs,divided into hypertrophic scars and keloids).These kinds of abnormal scars seriously reduce the quality of life of patients.However,their mechanism is still unclear,resulting in difficult clinical prevention,unstable treatment effects and a high risk of recurrence.Available evidence supports inflammatory changes caused by infection as one of the keys to abnormal proliferation of skin fibroblasts.In acne-induced PSs,increasing knowledge of the immunopathology indicates that inflammatory cells directly secrete growth factors to activate fibroblasts and release proinflammatory factors to promote the formation of PSs.T helper cells contribute to PSs via the secretion of interleukin(IL)-4 and IL-13,the pro-inflammatory factors;while regulatory T cells have anti-inflammatory effects,secrete IL-10 and prostaglandin E2,and suppress fibrosis production.Several treatments are available,but there is a lack of combination regimens to target different aspects of acne-induced PSs.Overall,this review indicates that the joint involvement of inflammatory response and fibrosis plays a crucial role in acne-induced PSs,and also analyzes the interaction of current treatments for acne and PS.
基金supported by the National Key Basic Research Program(973 Project)(No.2011CB933100)National Natural Scientific Fund(No.81225010,31100717)+1 种基金863 project of China(2012AA022703),Shanghai Science and Technology Fund(No.10XD1406100,and 13NM1401500)Shanghai Jiao Tong University Innovation Fund for Postgraduates(No.AE340011).
文摘A unique,sensitive,and highly specific fluoroimmunoassay system for antigen detection using quantum dot and gold nanoparticles has been developed.The assay is based on the fluorescence quenching of quantum dots caused by gold nanoparticles coated with antibody.To demonstrate its analytical capabilities,the quantum dots were coated with anti-HBsAg monoclonal antibodies(QDs-MAb1)and gold nanoparticles coated with another anti-HBsAg monoclonal antibodies(GNPs-MAb2)which specifically bound with HBsAg could sandwich the HBsAg captured by the immunoreactions.The sandwich-type immunocomplex was formed and the energy of quantum dots was transferred to gold nanoparticles as they were within a short distance,so that the fluorescence intensity of quantum dots was quenched.The fluorescence intensity of quantum dots at 570 nm was negative linear proportional to HBsAg concentration logarithm.The result showed that the limit of detection of the HBsAg was 0.928 ng/mL.This new system can be extended to detect target molecules with matched antibodies and has broad potential applications in immunoassay and disease diagnosis.
基金This study was supported by China Postdoctoral Science Foundation(Nos.2017M620159 and 2019T120345)National Natural Science Foundation of China(Nos.81772098 and 81801917),Shanghai Municipal Education Commission-Gaofeng Clinical Medicine Grant Support(No.20152227)+3 种基金Cross Research Project of Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine(No.JYJC202009)Shanghai Health Industry Clinical Research Special Project(No.20204Y0443)Shanghai Municipal Key Clinical Specialty(shslczdzk00901)Scientific Research Foundation of Shanghai Municipal Commission of Health and Family Planning(No.20154Y002).
文摘Photodynamic therapy(PDT)is an emerging therapeutic strategy for hypertrophic scars(HS),which is heavily dependent on reactive oxygen species(ROS)generation.However,the unsatisfactory delivery and excitation of 5-aminolevulinic acid(ALA,a commercial photosensitizer in dermatology)result in an insufficient ROS generation,and thus limit the clinical application of PDT treating HS(HS-PDT).Consequently,sophisticated transdermal co-delivery nanoethosomes(named A/A-ES)with ALA and Au nanotriangles(AuNTs)in cores are prepared via an in-situ seed-mediated growth method,and then applied to improve HS-PDT through localized surface plasmon resonance(LSPR)-enhanced ROS generation.A/A-ES display a satisfactory performance in co-delivery in HS tissue with sufficient protoporphyrin IX production and LSPR effect in cytoplasm,which is beneficial for ALA excitation as well as ROS generation.In vitrolvivo studies reveal that A/A-ES significantly improve HS-PDT in promoting to fibroblast apoptosis and collagen remodeling through LSPR-enhanced ROS generation.Therefore,this study provides a feasible strategy that integrates transdermal delivery and LSPR to enable the beneficial effects of HS-PDT through boosting the delivery and excitation of ALA.
基金This study was supported by the Special Fund of the National Science and Technology Major Project of China(No. 2018ZX10305409)Beijing Hospital Authority "Dengfeng" Talent Training Plan(DFL 20181201)Sanming Project of Medicine in Shenzhen(SZSM201512030)
文摘Importance: The current lack of reliable rapid tests for distinguishing between bacterial and viral infections has contributed to antibiotic misuse.Objective: This study aimed to develop a novel biomarker assay that integratesFAM89A andIFI44L measurements to assist in differentiating between bacterial and viral infections.Methods: This prospective study recruited children with febrile illness from two hospitals between July 1, 2018, and June 30, 2019. A panel of three experienced pediatricians performed reference standard diagnoses of all patients (i.e., bacterial or viral infection) using available clinical and laboratory data, including a 28-day follow-up assessment. Assay operators were blinded to the reference standard diagnoses. The expression levels ofFAM89A andIFI44L were determined by quantitative real-time polymerase chain reaction assessment.Results: Of 133 potentially eligible patients with suspected bacterial or viral infection, 35 were excluded after the application of exclusion criteria. The resulting cohort included 98 patients: 59 with viral diagnoses and 39 with bacterial diagnoses. The areas under the curve (AUCs) of diagnoses usingFAM89A andIFI44L were 0.694 [95% confidence interval (CI): 0.583-0.804] and 0.751 (95%CI: 0.651-0.851), respectively. The disease risk score (DRS) [log2(FAM89A expression) - log2(IFI44L expression)] signature achieved an improved area under the receiver operating characteristic curve (AUC, 0.825;95%CI: 0.735-0.915), compared with the AUC generated from individual host RNA. A combination of the DRS and the C-reactive protein (CRP) level achieved an AUC of 0.896 (95%CI: 0.825-0.966). Optimal cutoffs for the DRS and CRP level were -3.18 and 19.80 mg/L, respectively.Interpretation: The DRS was significantly more accurate than the CRP level in distinguishing between bacterial and viral infections;the combination of these two parameters exhibited greater sensitivity and specificity. This study provides information that could be useful for the clinical application ofFAM89A andIFI44L in terms of distinguishing between viral and bacterial infections.
基金supported by Key Basic Research Program of China(No.2017YFA0205304 and 2015CB931802)Innovative Team Project of Nature Scientific Foundation of China(No.81921002)Medical Engineering Cross Project of Shanghai Jiao Tong University(YG2017ZD12).
文摘The novel coronavirus pneumonia,a global pandemic disease named as coronavirus disease 2019,has caused enormous losses on the health and economies of people all over the world,while there is still a lack of quick and sensitive diagnostic method and effective therapy.Developing rapid diagnostic method for coronavirus disease 2019 has become exceptional urgent.Herein we report a rapid diagnostic method for the novel coronavirus through monitoring the volatile biomarkers in human exhaled breath.The breath volatile biomarkers are derived from the metabolism of novel coronavirus,including acetoin,2,4,6-trimethylpyridine,3-methyl tridecane,tetradecane,isooctyl alcohol,pentadecane,hexadecane,1-methylene-1H-indene.By comparing the types and concentrations of the volatile biomarkers in human exhaled breath combined with SERS sensor,we could distinguish between the healthy person and the patients with coronavirus disease 2019.This work confirms that various volatile organic compounds metabolized by novel coronavirus can be employed for rapidly screening of patients with coronavirus disease 2019,and has broad application prospects in the prevention and control of the epidemic.
