Spatiotemporal optical vortices(STOVs)exhibit characteristics of transverse orbital angular momentum(OAM)that is perpendicular to the direction of pulse propagation,indicating significant potential for diverse applica...Spatiotemporal optical vortices(STOVs)exhibit characteristics of transverse orbital angular momentum(OAM)that is perpendicular to the direction of pulse propagation,indicating significant potential for diverse applications.In this study,we employ vanadium dioxide and photonic crystal plates to design tunable transreflective dual-channel terahertz(THz)spatiotemporal vortex generation devices that possess multipolarization adaptability.In the reflection channel,we achieve active tunability of the topological dark lines by utilizing circularly polarized light,based on the topological dark phenomenon,and observe variations in the number of singularities across the parameter space from different observational perspectives.In the transmission channel,we generate independent vortex singularities using linearly polarized light.This multifunctional terahertz device offers a novel approach for the generation and active tuning of spatiotemporal vortices.展开更多
Immune checkpoint blockade(ICB)therapy potently revives T cell’s response to cancer.However,patients suffered with tumors that had inadequate infiltrated immune cells only receive limited therapeutic benefits from IC...Immune checkpoint blockade(ICB)therapy potently revives T cell’s response to cancer.However,patients suffered with tumors that had inadequate infiltrated immune cells only receive limited therapeutic benefits from ICB therapy.Synthetic biology promotes the alternative strategy of harnessing tumor-targeting bacteria to synthesize therapeutics to modulate immunity in situ.Herein,we engineered attenuated Salmonella typhimurium VNP20009 with gene circuits to synthetize granulocyte-macrophage colony-stimulating factor(GM-CSF)and interleukin 7(IL-7)within tumors,which recruited dendritic cells(DCs)and enhanced T cell priming to elicit anti-tumor response.The bacteria-produced GM-CSF stimulated the maturation of bone marrow-derived dendritic cells(BMDCs),while IL-7 promoted the proliferation of spleen isolated T cells and inhibited cytotoxicity T cell apoptosis in vitro.Virtually,engineered VNP20009 prefer to colonize in tumors,and inhibited tumor growth by enhancing DCs and T cell infiltration.Moreover,the tumor-toxic GZMB^(+)CD8^(+)T cell and IFN-γ^(+)CD8^(+)T cell populations conspicuously increased with the treatment of engineered bacteria.The combination of GM-CSF-IL-7-VNP20009 with PD-1 antibody synergistically stunted the tumor progress and metastasis.展开更多
基金National Natural Science Foundation of China(62375158,62105187)Natural Science Foundation of Shandong Province(ZR2021QF010,ZR2020MF107)Development Plan of Youth Innovation Team in Colleges and Universities of Shandong Province(2022KJ216)。
文摘Spatiotemporal optical vortices(STOVs)exhibit characteristics of transverse orbital angular momentum(OAM)that is perpendicular to the direction of pulse propagation,indicating significant potential for diverse applications.In this study,we employ vanadium dioxide and photonic crystal plates to design tunable transreflective dual-channel terahertz(THz)spatiotemporal vortex generation devices that possess multipolarization adaptability.In the reflection channel,we achieve active tunability of the topological dark lines by utilizing circularly polarized light,based on the topological dark phenomenon,and observe variations in the number of singularities across the parameter space from different observational perspectives.In the transmission channel,we generate independent vortex singularities using linearly polarized light.This multifunctional terahertz device offers a novel approach for the generation and active tuning of spatiotemporal vortices.
基金supported by grants from Shenzhen Science and Technology Program(Grant No.RCYX20200714114643121)The National Natural Science Foundation of China(31971268,32201084,32371425)+7 种基金Science,Technology&Innovation Commission of Shenzhen Municipality(JCYJ20200109142610136,JCYJ20180507181654186,ZDSYS20220606100803007)Guangdong Basic and Applied Basic Research Foundation(2019A1515010855,2020A1515110166)the Natural Science Foundation of Guangdong Province(No.2020A1515010802,No.2022A1515012289)University of Chinese Academy of Sciences-Shenzhen Hospital Research Funding(HRF-2020004)the Health system scientific research project of Shenzhen Guangming District Science and innovation Bureau(2020R01073,2020R01061)Fundamental Research Funds for the Central Universities(19lgzd453)Special fund for economic development of ShenZhen Guangming District(2021R01128)Doctoral personnel scientific research start-up Fund project of Guangdong Medical University(GDMUB2022037).
文摘Immune checkpoint blockade(ICB)therapy potently revives T cell’s response to cancer.However,patients suffered with tumors that had inadequate infiltrated immune cells only receive limited therapeutic benefits from ICB therapy.Synthetic biology promotes the alternative strategy of harnessing tumor-targeting bacteria to synthesize therapeutics to modulate immunity in situ.Herein,we engineered attenuated Salmonella typhimurium VNP20009 with gene circuits to synthetize granulocyte-macrophage colony-stimulating factor(GM-CSF)and interleukin 7(IL-7)within tumors,which recruited dendritic cells(DCs)and enhanced T cell priming to elicit anti-tumor response.The bacteria-produced GM-CSF stimulated the maturation of bone marrow-derived dendritic cells(BMDCs),while IL-7 promoted the proliferation of spleen isolated T cells and inhibited cytotoxicity T cell apoptosis in vitro.Virtually,engineered VNP20009 prefer to colonize in tumors,and inhibited tumor growth by enhancing DCs and T cell infiltration.Moreover,the tumor-toxic GZMB^(+)CD8^(+)T cell and IFN-γ^(+)CD8^(+)T cell populations conspicuously increased with the treatment of engineered bacteria.The combination of GM-CSF-IL-7-VNP20009 with PD-1 antibody synergistically stunted the tumor progress and metastasis.
