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瓜氨酸化α-烯醇化酶特异性单克隆抗体制备及其在阿尔茨海默病动物模型中的检测应用 被引量:1
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作者 韩魏巍 胡梦蝶 +6 位作者 苏伊玲 邓灵福 袁永泽 杨洋 熊丽 刘徳立 耿辉 《现代免疫学》 CSCD 北大核心 2017年第3期206-211,共6页
本研究利用杂交瘤技术制备瓜氨酸化α-烯醇化酶特异性单克隆抗体,并探究瓜氨酸化α-烯醇化酶抗体在检测阿尔茨海默病(AD)动物模型中的作用。利用生物信息学软件分析预测α-烯醇化酶的B细胞优势表位,选取~9R-^(25)F位氨基酸序列,将该表... 本研究利用杂交瘤技术制备瓜氨酸化α-烯醇化酶特异性单克隆抗体,并探究瓜氨酸化α-烯醇化酶抗体在检测阿尔茨海默病(AD)动物模型中的作用。利用生物信息学软件分析预测α-烯醇化酶的B细胞优势表位,选取~9R-^(25)F位氨基酸序列,将该表位多肽中2个精氨酸替换为瓜氨酸,化学合成瓜氨酸化α-烯醇化酶~9R-^(25)F多肽(citrullinatedα-enolase peptide,CEP)。采取活泼酯法将CEP多肽与BSA偶联制备CEP-BSA偶联抗原并免疫BALB/c小鼠,杂交瘤技术制备CEP单克隆抗体。免疫组织化学和Western blotting对其特异性进行验证。CEP-BSA偶联抗原免疫小鼠能诱发特异性抗体反应,经细胞融合、筛选得到一株稳定分泌CEP特异性单克隆抗体的杂交瘤细胞,命名为9B5。Western blotting分析显示该单克隆抗体能特异性结合瓜氨酸化α-烯醇化酶,免疫组织化学检测显示9B5单抗能结合AD模型大鼠脑组织细胞上瓜氨酸化的α-烯醇化酶。本研究成功制备的瓜氨酸化α-烯醇化酶特异性单克隆抗体将有助于进一步研究瓜氨酸化α-烯醇化酶在相关疾病发病中的作用。 展开更多
关键词 抗原优势表位 瓜氨酸化 α-烯醇化酶 单克隆抗体
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Effect of Salt Stress on Ammonium Assimilation Enzymes of the Roots of Rice(Oryza sativa)Cultivars Differing in Salinity Resistance 被引量:9
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作者 ZHOU Wei SUN Qing-Jie +3 位作者 ZHANG Chu-Fu yuan yong-ze ZHANG Ji LU Bin-Bin 《Acta Botanica Sinica》 CSCD 2004年第8期921-927,共7页
Effects of NaCI-stress on ammonia assimilation enzymes and the related parameters were determined in the roots of three rice(Oryza sativa L.)cultivars differing in salt tolerance.The results showed that the activities... Effects of NaCI-stress on ammonia assimilation enzymes and the related parameters were determined in the roots of three rice(Oryza sativa L.)cultivars differing in salt tolerance.The results showed that the activities of glutamine synthetase(GS)and NADH-dependent glutamate synthase(NADH-GOGAT),as well as the levels of soluble protein decreased under high concentration salt.The influence extent was in accordance with Zao-hua 2(salt-sensitive),Jin-zhu 1(normal cultivar)and Jin-dao 779(salt-resistant),which was consistent with their salt-tolerance.Nevertheless,under the stress of high salt concentration,NADH-dependent glutamate dehydrogenase(NADH-GDH)activity of Zao-hua 2 and Jin-zhu1 was induced significantly,but that of Jin-dao 779 did not increase remarkably.The salt stress led to the accumulation of total soluble sugar(TSS)in the root of Jin-zhu 1 and Jin-dao 779 in different degree.The level of TSS in Zao-hua 2 changed with different NaCl concentrations.Among the cultivars observed,content of proline increased in different degree,but under high salt concentrations,it had a more marked rise in the roots of salt-sensitive cultivars. 展开更多
关键词 glutamine synthetase(GS) glutamate synthase(GOGAT) glutamate dehydrogenase(GDH) NICE salt stress
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