To investigate the characters of repetitive DNA sequence in the sex chromosomes of the spiny eel （Mastacembelus aculeatus）, the X chromosomal library was screened and a family of repetitive sequence, consisting of M...To investigate the characters of repetitive DNA sequence in the sex chromosomes of the spiny eel （Mastacembelus aculeatus）, the X chromosomal library was screened and a family of repetitive sequence, consisting of Ma 1-Ma 6, was isolated. The fluorescence in situ hybridization （FISH） result confirmed that Ma 1- Ma 5 dispersed over sex chromosomes and all autosomes, whereas, Ma 6 is sex chromosome-specific and distributed only on the C-band positive regions of X chromosome, and Ma 6 maybe the main components of the heterochromatic regions of X chromosome. This study provides additional information about the evolution of sex chromosomes in lower vertebrates such as fish.展开更多
Using the primers which specially amplify the conservative motif of Human SRYgene,we stud ied the PCR amplification of Sox genes in genomic DNA of two species of mud loach Misgurus anguillicau-datus and Paramisgurnus ...Using the primers which specially amplify the conservative motif of Human SRYgene,we stud ied the PCR amplification of Sox genes in genomic DNA of two species of mud loach Misgurus anguillicau-datus and Paramisgurnus dabryanus.Four bands with the length of 200,550,940 and 1000 bp respectively,were presented in the PCR products of Misgumus anguillicaudatus.Three bands with the length of 200,550 and 900 bp were presented in that of Paramisgumus dabryamus.Southern blotting results indicated that the 200 and 550 bp bands are specially positive.There is no difference between male and female individuals as well as between these two species.展开更多
A wealth of literature has been published in the studies of Chinese fresh-water fish chromosomes within recent years.In this paper,we review the inter-and intraspecific genetic diversities found in Chinese fresh-water...A wealth of literature has been published in the studies of Chinese fresh-water fish chromosomes within recent years.In this paper,we review the inter-and intraspecific genetic diversities found in Chinese fresh-water fishes,and explore the characteristics and mechanisms that underline these diversities and variations. We also propose the hypothesis how the genetic diversities had evolved in the fresh-water fishes.展开更多
Well spread pachytene bivalents with high-resolution multiple bands of zebrafish were obtained after the testes were treated with alkaline hypotonic solution and high chloroform fixative solution.This might be the pat...Well spread pachytene bivalents with high-resolution multiple bands of zebrafish were obtained after the testes were treated with alkaline hypotonic solution and high chloroform fixative solution.This might be the pattern with the largest number of multiple bands obtained from fish chromosomes so far published.Both the number and character of the bands in each bivalent were stable.According to the principles of ISCN(1978)and ISCN(1981),an idiogram of 599 bands was set up,and the detailed description of the landmark system and the band positions were given.展开更多
基金Supported by the National Natural Science Foundation of China(30400044)
文摘To investigate the characters of repetitive DNA sequence in the sex chromosomes of the spiny eel （Mastacembelus aculeatus）, the X chromosomal library was screened and a family of repetitive sequence, consisting of Ma 1-Ma 6, was isolated. The fluorescence in situ hybridization （FISH） result confirmed that Ma 1- Ma 5 dispersed over sex chromosomes and all autosomes, whereas, Ma 6 is sex chromosome-specific and distributed only on the C-band positive regions of X chromosome, and Ma 6 maybe the main components of the heterochromatic regions of X chromosome. This study provides additional information about the evolution of sex chromosomes in lower vertebrates such as fish.
文摘Using the primers which specially amplify the conservative motif of Human SRYgene,we stud ied the PCR amplification of Sox genes in genomic DNA of two species of mud loach Misgurus anguillicau-datus and Paramisgurnus dabryanus.Four bands with the length of 200,550,940 and 1000 bp respectively,were presented in the PCR products of Misgumus anguillicaudatus.Three bands with the length of 200,550 and 900 bp were presented in that of Paramisgumus dabryamus.Southern blotting results indicated that the 200 and 550 bp bands are specially positive.There is no difference between male and female individuals as well as between these two species.
基金State Education Commission Research Foundation for Doctoral Disciplines
文摘A wealth of literature has been published in the studies of Chinese fresh-water fish chromosomes within recent years.In this paper,we review the inter-and intraspecific genetic diversities found in Chinese fresh-water fishes,and explore the characteristics and mechanisms that underline these diversities and variations. We also propose the hypothesis how the genetic diversities had evolved in the fresh-water fishes.
基金This work was supported by the National Natural Science Foundation of China(Grant Nos.30170508&39970406)the Research Fund for the Doctoral Program Foundation of Higher Education(Grant No.98048628).
文摘Well spread pachytene bivalents with high-resolution multiple bands of zebrafish were obtained after the testes were treated with alkaline hypotonic solution and high chloroform fixative solution.This might be the pattern with the largest number of multiple bands obtained from fish chromosomes so far published.Both the number and character of the bands in each bivalent were stable.According to the principles of ISCN(1978)and ISCN(1981),an idiogram of 599 bands was set up,and the detailed description of the landmark system and the band positions were given.
