Background:Long noncoding RNA,LINC01106 exhibits high expression in lung adenocarcinoma(LUAD)tumor tissues,but its functional role and regulatory mechanism in LUAD cells remain unclear.Methods:LINC01106 expression was...Background:Long noncoding RNA,LINC01106 exhibits high expression in lung adenocarcinoma(LUAD)tumor tissues,but its functional role and regulatory mechanism in LUAD cells remain unclear.Methods:LINC01106 expression was analyzed in LUAD tissues and its functional impact on LUAD cells was assessed.LUAD cells were silenced with sh-LINC01106 and injected into nude mice to investigate tumor growth.The downstream transcription factors and molecular mechanism were determined using the Human transcription factor database(TFDB)database and Gene Expression Profiling Interactive Analysis(GEPIA)database.Additionally,the impact of linc01106 on autophagy was analyzed by determining the expression of autophagy-related genes(ATGs)in LUAD cells.Results:Our results showed that LINC01106 exhibited upregulation in both LUAD tissues and cell lines.The silencing of LINC01106 demonstrated a suppressive effect on tumorigenesis in a xenograft mouse model of LUAD.Additionally,LINC01106 was found to recruit TATA-binding protein-associated factor 15(TAF15),an RNA-binding protein,thereby enhancing the mRNA stability of TEA domain transcription factor 4(TEAD4).In turn,TEAD4 served as a transcription factor that bound to the LINC01106 promoter and regulated its expression.Further assays indicated that LINC01106 promoted autophagy in LUAD cells by upregulating the expression of autophagy-related genes(ATGs).The silencing of LINC01106 in LUAD cells inhibited autophagy,and cell proliferation,and promoted apoptosis,which all were effectively reversed by ATG5 overexpression.Conclusions:Overall,LINC01106,transcriptionally activated by TEAD4,interacts with TAF15 to promote the stability of TEAD4 and upregulates the expression of ATGs,promoting malignancy of LUAD cells.展开更多
The external hard TiN ceramic layer and an internal reinforced NiTi matrix were controlled by combining nitriding and solution treatment to achieve ultra-high micro-hardness and excellent wear performance.In addition,...The external hard TiN ceramic layer and an internal reinforced NiTi matrix were controlled by combining nitriding and solution treatment to achieve ultra-high micro-hardness and excellent wear performance.In addition,the effects of nitriding and solution treatment on the microstructure evolution,martensitic transformation,and mechanical and functional properties of 60NiTi alloys were investigated.Compared with 60NiTi alloy,a harder TiN layer appeared at the surface of 60NiTi alloy with nitriding treatment.Meanwhile,a Ni_(3)Ti layer formed under the bottom of TiN layer owing to the consumption of Ti in matrix induced by formation of TiN layer.Upon undergoing solution treatment,the precipitates evolved from the coexistence of Ni_(3)Ti and Ni_(3)Ti_(2)phase to Ni_(4)Ti_(3)phase.The introduction of the outer harder TiN ceramic layer had a constraint of martensitic transformation of 60NiTi alloy,which led to the reduction of martensitic transformation temperature(Ms)and the suppression of martensitic transformation.Importantly,the construction of outermost harder TiN ceramic layer at the surface of B2-NiTi matrix enhanced Ni_(4)Ti_(3)phase improved significantly the microhardness and wear performances by the nitriding and followed solution treatment.Meanwhile,the high compressive fracture strength,fracture strain and superior superelasticity of 60NiTi alloy can be maintained.展开更多
The key of tissue section technique is the selection and application of the embedding agent. Based on the specific characteristics of the tissue and outer skeleton of insects, a new kind of embedding agent, colophony ...The key of tissue section technique is the selection and application of the embedding agent. Based on the specific characteristics of the tissue and outer skeleton of insects, a new kind of embedding agent, colophony and paraffin (CP), was used without destroying the integrity of insect organs. The tissue can be sliced continuously by the use of CP embedding technique. It solves the 3 difficult problems in the serial section of the organ and tissue of insect, namely stiff materials, thin section and continuity of sections. These difficult problems are not solved by any unitary tissue section technique that is used now, including the paraffin section technique. By using the CP embedding technique, any parts or organs (like antenna, leg and wing) and even the whole body of insects (mini body) can be sliced up continuously. It will open up wide possibilities for the study of inner structure, the interrelations among organs, the micro-characteristics and the physiological functions of insects展开更多
基金supported by the 2022 Natural Science Foundation of Fujian Province(No.2022J011486).
文摘Background:Long noncoding RNA,LINC01106 exhibits high expression in lung adenocarcinoma(LUAD)tumor tissues,but its functional role and regulatory mechanism in LUAD cells remain unclear.Methods:LINC01106 expression was analyzed in LUAD tissues and its functional impact on LUAD cells was assessed.LUAD cells were silenced with sh-LINC01106 and injected into nude mice to investigate tumor growth.The downstream transcription factors and molecular mechanism were determined using the Human transcription factor database(TFDB)database and Gene Expression Profiling Interactive Analysis(GEPIA)database.Additionally,the impact of linc01106 on autophagy was analyzed by determining the expression of autophagy-related genes(ATGs)in LUAD cells.Results:Our results showed that LINC01106 exhibited upregulation in both LUAD tissues and cell lines.The silencing of LINC01106 demonstrated a suppressive effect on tumorigenesis in a xenograft mouse model of LUAD.Additionally,LINC01106 was found to recruit TATA-binding protein-associated factor 15(TAF15),an RNA-binding protein,thereby enhancing the mRNA stability of TEA domain transcription factor 4(TEAD4).In turn,TEAD4 served as a transcription factor that bound to the LINC01106 promoter and regulated its expression.Further assays indicated that LINC01106 promoted autophagy in LUAD cells by upregulating the expression of autophagy-related genes(ATGs).The silencing of LINC01106 in LUAD cells inhibited autophagy,and cell proliferation,and promoted apoptosis,which all were effectively reversed by ATG5 overexpression.Conclusions:Overall,LINC01106,transcriptionally activated by TEAD4,interacts with TAF15 to promote the stability of TEAD4 and upregulates the expression of ATGs,promoting malignancy of LUAD cells.
基金supported by the Development Plan of Shandong Province Young Innovation Team of Higher Education Institutions,China(No.2023KJ242)the National Natural Science Foundation of China(Nos.52371181,52101231,52101232,and 52175192)the Natural Science Foundation of Shandong Province,China(Nos.ZR2024QE019,and ZR2021QE044)。
文摘The external hard TiN ceramic layer and an internal reinforced NiTi matrix were controlled by combining nitriding and solution treatment to achieve ultra-high micro-hardness and excellent wear performance.In addition,the effects of nitriding and solution treatment on the microstructure evolution,martensitic transformation,and mechanical and functional properties of 60NiTi alloys were investigated.Compared with 60NiTi alloy,a harder TiN layer appeared at the surface of 60NiTi alloy with nitriding treatment.Meanwhile,a Ni_(3)Ti layer formed under the bottom of TiN layer owing to the consumption of Ti in matrix induced by formation of TiN layer.Upon undergoing solution treatment,the precipitates evolved from the coexistence of Ni_(3)Ti and Ni_(3)Ti_(2)phase to Ni_(4)Ti_(3)phase.The introduction of the outer harder TiN ceramic layer had a constraint of martensitic transformation of 60NiTi alloy,which led to the reduction of martensitic transformation temperature(Ms)and the suppression of martensitic transformation.Importantly,the construction of outermost harder TiN ceramic layer at the surface of B2-NiTi matrix enhanced Ni_(4)Ti_(3)phase improved significantly the microhardness and wear performances by the nitriding and followed solution treatment.Meanwhile,the high compressive fracture strength,fracture strain and superior superelasticity of 60NiTi alloy can be maintained.
文摘The key of tissue section technique is the selection and application of the embedding agent. Based on the specific characteristics of the tissue and outer skeleton of insects, a new kind of embedding agent, colophony and paraffin (CP), was used without destroying the integrity of insect organs. The tissue can be sliced continuously by the use of CP embedding technique. It solves the 3 difficult problems in the serial section of the organ and tissue of insect, namely stiff materials, thin section and continuity of sections. These difficult problems are not solved by any unitary tissue section technique that is used now, including the paraffin section technique. By using the CP embedding technique, any parts or organs (like antenna, leg and wing) and even the whole body of insects (mini body) can be sliced up continuously. It will open up wide possibilities for the study of inner structure, the interrelations among organs, the micro-characteristics and the physiological functions of insects
