Bacterial biofilms,especially those caused by multidrug-resistant bacteria,have emerged as one of the greatest dangers to global public health.The acceleration of antimicrobial resistance to conventional an-tibiotics ...Bacterial biofilms,especially those caused by multidrug-resistant bacteria,have emerged as one of the greatest dangers to global public health.The acceleration of antimicrobial resistance to conventional an-tibiotics and the severe lack of new drugs necessitates the development of novel agents for biofilm eradication.Photodynamic therapy(PDT)is a promising non-antibiotic method for treating bacterial infections.However,its application in biofilm eradication is hampered by the hypoxic microenvironment of biofilms and the physical protection of extracellular polymeric substances.In this study,we develop a composite nanoplatform with oxygen(O_(2))self-supplying and heat-sensitizing capabilities to improve the PDT efficacy against biofilms.CaO_(2)/ICG@PDA nanoparticles(CIP NPs)are fabricated by combining calcium peroxide(CaO_(2))with the photosensitizer indocyanine green(ICG)via electrostatic interactions,followed by coating with polydopamine(PDA).The CIP NPs can gradually generate O_(2)in response to the acidic microenvironment of the biofilm,thereby alleviating its hypoxic state.Under near-infrared(NIR)irradiation,the nanoplatform converts O_(2)into a significant amount of singlet oxygen(^(1)O_(2))and heat to eradicate biofilm.The generated heat enhances the release of O_(2),accelerates the generation of^(1)O_(2)in PDT,increases cell membrane permeability,and increases bacterial sensitivity to^(1)O_(2).This nanoplatform significantly improves the efficacy of PDT in eradicating biofilm-dwelling bacteria without fostering drug resistance.Experiments on biofilm eradication demonstrate that this nanoplatform can eradicate over 99.9999%of methicillin-resistant Staphylococcus aureus(MRSA)biofilms under 5-min NIR irradiation.Notably,these integrated advantages enable the system to promote the healing of MRSA biofilm-infected wounds with negligible toxicity in vivo,indicating great promise for overcoming the obstacles associated with bacterial biofilm eradication.展开更多
Single-stranded DNA-binding proteins(SSBs)play essential roles in the replication,recombination and repair processes of organellar DNA molecules.In Arabidopsis thaliana,SSBs are encoded by a small family of two genes(...Single-stranded DNA-binding proteins(SSBs)play essential roles in the replication,recombination and repair processes of organellar DNA molecules.In Arabidopsis thaliana,SSBs are encoded by a small family of two genes(SSB1 and SSB2).However,the functional divergence of these two SSB copies in plants remains largely unknown,and detailed studies regarding their roles in the replication and recombination of organellar genomes are still incomplete.In this study,phylogenetic,gene structure and protein motif analyses all suggested that SSB1 and SSB2 probably diverged during the early evolution of seed plants.Based on accurate long-read sequencing results,ssb1 and ssb2 mutants had decreased copy numbers for both mitochondrial DNA(mtDNA)and plastid DNA(ptDNA),accompanied by a slight increase in structural rearrangements mediated by intermediate-sized repeats in mt genome and small-scale variants in both genomes.Our findings provide an important foundation for further investigating the effects of DNA dosage in the regulation of mutation frequencies in plant organellar genomes.展开更多
Facilitating anion redox chemistry is an effective strategy to increase the capacity of layered oxides for sodium-ion batteries.Nevertheless,there remains a paucity of literature pertaining to the oxygen redox chemist...Facilitating anion redox chemistry is an effective strategy to increase the capacity of layered oxides for sodium-ion batteries.Nevertheless,there remains a paucity of literature pertaining to the oxygen redox chemistry of O3-type layered oxide cathode materials.This work systematically investigates the effect of Fe doping on the anionic oxygen redox chemistry and electrochemical reactions in O3-NaNi_(0.4)Cu_(0.1)Mn_(0.4)Ti_(0.1)O_(2).The results of the density functional theory(DFT)calculations indicate that the electrons of the O 2p occupy a higher energy level.In the ex-situ X-ray photoelectron spectrometer(XPS)of O 1s,the addition of Fe facilitates the lattice oxygen(O^(n-))to exhibit enhanced activity at 4.45 V.The in-situ X-ray diffraction(XRD)demonstrates that the doping of Fe effectively suppresses the Y phase transition at high voltages.Furthermore,the Galvanostatic Intermittent Titration Technique(GITT)data indicate that Fe doping significantly increases the Na~+migration rate at high voltages.Consequently,the substitution of Fe can elevate the cut-off voltage to 4.45 V,thereby facilitating electron migration from O^(2-).The redox of O^(2-)/O^(n-)(n<2)contributes to the overall capacity.O3-Na(Ni_(0.4)Cu_(0.1)Mn_(0.4)Ti_(0.1))_(0.92)Fe_(0.08)O_(2)provides an initial discharge specific capacity of 180.55 mA h g^(-1)and71.6%capacity retention at 0.5 C(1 C=240 mA g^(-1)).This work not only demonstrates the beneficial impact of Fe substitution for promoting the redox activity and reversibility of O^(2-)in 03-type layered oxides,but also guarantees the structural integrity of the cathode materials at high voltages(>4.2 V).It offers a novel avenue for investigating the anionic redox reaction in O3-type layered oxides to design advanced cathode materials.展开更多
Due to the absence of timely and effective therapies,infections induced by bacterial biofilms have been widely acknowledged as a significant global public health concern.In modern times,aside from surgical interventio...Due to the absence of timely and effective therapies,infections induced by bacterial biofilms have been widely acknowledged as a significant global public health concern.In modern times,aside from surgical intervention(when appropriate),antibiotics are the sole clinical option for treating biofilm-associated infections.However,the rise of drug resistance,as well as the poor therapeutic effects of current treatment regimens in eliminating biofilms highlight the requirement for novel strategies to enhance the accessibility of antibiotics in the"post-antibiotic era".The current study presents a multifunctional nanoplatform equipped with a"disruption and killing"function to enhance the effectiveness of conventional antibiotics for the eradication of biofilms.Herein,mesoporous silica nanoparticles were employed as carriers to encapsulate the model antibiotic rifampicin(Rif).Subsequently,the nanoparticles were coated with layers of the tannic acid/iron ion(TA/Fe)complex and immobilized with α-amylase.The α-amylase present in the outer layer can degrade the polysaccharides of extracellular polymeric substances(EPS),which in turn disrupts the structural integrity of the biofilms,thus facilitating the entry of the nanoplatform.When exposed to near-infrared(NIR)light,the TA/Fe complex layers can generate heat,which facilitates the release of Rif and increases the bacterial uptake of Rif by damaging the bacterial cell membrane,ultimately resulting in the elimination of bacteria within biofilms.The in vitro experiments demonstrated that this nanoplatform effectively eliminated over 99% of biofilms formed by Staphylococcus aureus and Pseudomonas aeruginosa when exposed to NIR radiation for 10 min.Additionally,in vivo experimental findings further validated the extensive therapeutic efficacy of this nanoplatform against biofilm-infected wounds,accelerating the rate of healing and reducing inflammatory reactions.To summarize,this nanoplatform provides a novel avenue to improve the effectiveness of conventional antibiotics in eradicating bacterial biofilms.展开更多
Osteoarthritis(OA),the most prevalent degenerative joint disease,is marked by cartilage degradation and pathological alterations in surrounding tissues.Currently,no effective disease-modifying treatments exist.This st...Osteoarthritis(OA),the most prevalent degenerative joint disease,is marked by cartilage degradation and pathological alterations in surrounding tissues.Currently,no effective disease-modifying treatments exist.This study aimed to elucidate the critical roles of Myb-like,SWIRM,and MPN domains 1(MYSM1)and its downstream effector,Receptor-interacting protein kinase 2(RIPK2),in OA pathogenesis and the underlying mechanisms.Our findings revealed reduced MYSM1 levels in the cartilage of OA patients and mouse models.Genetic or adenovirus-induced MYSM1 knockout exacerbated OA progression in mice,whereas MYSM1 overexpression mitigated it.Mechanistically,MYSM1 inhibited the NF-κB and MAPK signaling pathways.Conversely,downstream RIPK2 significantly increased OA-like phenotypes and activated the NF-κB and MAPK pathways.The Ripk2^(S176D) mutation accelerated OA pathogenesis,while Ripk2 silencing or Ripk2^(S176A)mutation deactivated NF-κB and MAPK pathways,counteracting the role of MYSM1.MYSM1 deubiquitinates and dephosphorylates RIPK2^(S176)by recruiting protein phosphatase 2 A(PP2A).These results suggest that targeting MYSM1 or downstream RIPK2 offers promising therapeutic potential for OA.展开更多
BACKGROUND Hypoxemia due to respiratory depression and airway obstruction during upper gastrointestinal endoscopy with sedation is a common concern.The Wei nasal jet tube(WNJT)is a new nasopharyngeal airway with the a...BACKGROUND Hypoxemia due to respiratory depression and airway obstruction during upper gastrointestinal endoscopy with sedation is a common concern.The Wei nasal jet tube(WNJT)is a new nasopharyngeal airway with the ability to provide supraglottic jet ventilation and oxygen insufflation via its built-in wall channel.The available evidence indicates that with a low oxygen flow,compared with nasal cannula,the WNJT does not decrease the occurrence of hypoxemia during upper gastrointestinal endoscopy with propofol sedation.To date,there has been no study assessing the performance of WNJT for supplemental oxygen during upper gastrointestinal endoscopy with sedation when a moderate oxygen flow is used.AIM To determine whether the WNJT performs better than the nasal prongs for the prevention of hypoxemia during gastroscopy with propofol mono-sedation when a moderate oxygen flow is provided in patients with a normal body mass index.METHODS This study was performed in 291 patients undergoing elective gastroscopy with propofol mono-sedation.Patients were randomized into one of two groups to receive either the WNJT(WNJT group,n=147)or the nasal cannula(nasal cannula group,n=144)for supplemental oxygen at a 5-L/min flow during gastroscopy.The lowest SpO2 during gastroscopy was recorded.The primary endpoint was the incidence of hypoxemia or severe hypoxemia during gastroscopy.RESULTS The total incidence of hypoxemia and severe hypoxemia during gastroscopy was significantly decreased in the WNJT group compared with the nasal cannula group(P=0.000).The lowest median SpO2 during gastroscopy was significantly higher(98%;interquartile range,97-99)in the WNJT group than in the nasal cannula group(96%;interquartile range,93-98).Epistaxis by device insertion in the WNJT group occurred in 7 patients but stopped naturally without any treatment.The two groups were comparable in terms of the satisfaction of physicians,anesthetists and patients.CONCLUSION With a moderate oxygen flow,the WNJT is more effective for the prevention of hypoxemia during gastroscopy with propofol mono-sedation compared with nasal prongs,but causing slight epistaxis in a few patients.展开更多
Attachment of bacteria and subsequent formation of biofilms on material surfaces lead to serious consequences including infection,contamination and biofouling,posing a prominent threat to human health and causing prob...Attachment of bacteria and subsequent formation of biofilms on material surfaces lead to serious consequences including infection,contamination and biofouling,posing a prominent threat to human health and causing problems in many industries.Therefore,it is highly desirable to endow the surfaces with antibacterial properties.Traditional antibacterial surfaces are designed via either bacteria-resisting strategy to prevent the initial adhesion of bacteria or bacteria-killing strategy to eradicate any bacteria that attach to the surface.However,these single-function surfaces have their inherent shortcomings and cannot realize long-term efficacy against bacteria.In recent years,various dual-function antibacterial surfaces with both bacteria-resisting and bacteria-killing properties together have been developed,showing better performance for combating surface-attached bacteria and preventing formation of biofilms.In this review,we summarize the recent development of these dual-function antibacterial surfaces.We focus on the design principles and fabrication strategies of such surfaces and highlight the representative examples,which are categorized specifically into two types according to the anti-adhesive and bactericidal properties are simultaneous or switchable.A brief perspective is finally presented on current challenges and future research directions.展开更多
Phrenic nerve transfer is a major dynamic treatment used to repair brachial plexus root avulsion. We analyzed 72 relevant articles on phrenic nerve transfer to repair injured brachial plexus that were indexed by Scien...Phrenic nerve transfer is a major dynamic treatment used to repair brachial plexus root avulsion. We analyzed 72 relevant articles on phrenic nerve transfer to repair injured brachial plexus that were indexed by Science Citation Index. The keywords searched were brachial plexus injury, phrenic nerve, repair, surgery, protection, nerve transfer, and nerve graft. In addition, we per-formed neurophysiological analysis of the preoperative condition and prognosis of 10 patients undergoing ipsilateral phrenic nerve transfer to the musculocutaneous nerve in our hospital from 2008 to 201 3 and observed the electromyograms of the biceps brachii and motor conduc-tion function of the musculocutaneous nerve. Clinically, approximately 28% of patients had brachial plexus injury combined with phrenic nerve injury, and injured phrenic nerve cannot be used as a nerve graft. After phrenic nerve transfer to the musculocutaneous nerve, the regener-ated potentials ifrst appeared at 3 months. Recovery of motor unit action potential occurred 6 months later and became more apparent at 12 months. The percent of patients recovering ‘ex-cellent’ and ‘good’ muscle strength in the biceps brachii was 80% after 18 months. At 12 months after surgery, motor nerve conduction potential appeared in the musculocutaneous nerve in seven cases. These data suggest that preoperative evaluation of phrenic nerve function may help identify the most appropriate nerve graft in patients with an injured brachial plexus. The func-tional recovery of a transplanted nerve can be dynamically observed after the surgery.展开更多
In this paper, we propose a dual-threshold based secure On-Off transmission scheme, where signals are transmitted only if the channel condition can guarantee secure and reliable communication. First, we present a dyna...In this paper, we propose a dual-threshold based secure On-Off transmission scheme, where signals are transmitted only if the channel condition can guarantee secure and reliable communication. First, we present a dynamic access strategy to increase access efficiency, which provides an access region for the intended user. Then, we propose an emission control policy to transmit signals according to the current channel condition, which declines the influence of channel estimation errors and guarantees qualities of communication links. Furthermore, we give a comprehensive performance analysis for the proposed scheme in terms of connection outage probability(COP) and secrecy outage probability(SOP), and present a dual-threshold optimization model to further support the performance. Numerical results verify that the secure On-Off transmission scheme can increase the system secure energy efficiency and guarantee reliable and secure communication.展开更多
For most traditional wound dressings,it is challenging to simultaneously eliminate bacteria and promote angiogenesis to accelerate the healing process of bacteria-infected wounds.In this work,we develop a multifunctio...For most traditional wound dressings,it is challenging to simultaneously eliminate bacteria and promote angiogenesis to accelerate the healing process of bacteria-infected wounds.In this work,we develop a multifunctional dressing based on bacterial cellulose(BC)deposited with a tannic acid/Cu^(2+)ion/Mg^(2+)ion(TCM)complex film.Overall,the TCM complex exhibits robust interfacial adhesion to modify BC and good photothermal properties to effectively eradicate bacteria in the wound area under near-infrared(NIR)irradiation.The individual components of the TCM complex have several advantageous features for wound healing,such as antibacterial ability and negligible cytotoxicity;in particular,the released Cu^(2+)and Mg^(2+)ions are favorable for the proliferation,migration,and tube formation of endothelial cells in vitro.The results of in vivo experiments demonstrated that with the assistance of NIR irradiation,this composite dressing is more effective than traditional gauze or pristine BC dressing in promotion of angiogenesis and collagen deposition without causing remarkable inflammation,thereby accelerating the healing process of bacteria-infected full-thickness skin wounds.This work thus provides a simple and facile way to fabricate multifunctional BC-based dressings that could be potentially used for treating infected wounds.展开更多
Bacterial biofilms formed on the material surfaces have posed a series of serious problems for human health and industries.The treatment of mature biofilms is particularly difficult because they are inher-ently highly...Bacterial biofilms formed on the material surfaces have posed a series of serious problems for human health and industries.The treatment of mature biofilms is particularly difficult because they are inher-ently highly resistant against antibiotics and other adverse factors.The prevention is strategically advan-tageous over the treatment,and thus the development of innovative surfaces with capability to inhibit biofilm formation is highly demanded.In this work,we developed a superhydrophobic photothermal coating for prevention of biofilm formation,which was based on candle soot with hierarchical structure and excellent light-to-heat conversion ability.This coating was fabricated by deposition of a candle soot layer on the substrate,followed by sequential chemical vapor deposition of tetraethoxysilane and immo-bilization of fluorinated silane to make the coating robust and superhydrophobic.The resulted coating could repel a majority of bacteria from the surface at the early stage,and then eradicate a small number of bacteria remained on the surface under a short-term irradiation of near-infrared laser.The combi-nation of anti-adhesive property and photothermal bactericidal property endowed the coating with good antibiofilm property to prevent biofilm formation for at least 2 weeks.This coating is facile for deposition on various substrates with good storage stability,showing great potential for diverse practical applications to solve the biofilm-associated problems of materials and devices.展开更多
Brain is the most complex organ of human beings. The complete understanding of human brains＇ functions is an ongoing challenge for neuroscience. Although there are many techniques used for brain research, neuroscient...Brain is the most complex organ of human beings. The complete understanding of human brains＇ functions is an ongoing challenge for neuroscience. Although there are many techniques used for brain research, neuroscientists, physicists, and engineers are still exploring other access of research for applications. Terahertz （THz） spectroscopy and imaging may create such a space for brain research. THz spectroscopic technique, which is a novel medical imaging modality, attracts attention of biomedical applications due to its unique features including non-invasive, non-ionizing, and high sensitivity to biomolecules and tissues. In this article, we introduce our recent investigations on neuroscience using THz techniques, meanwhile, introduce other＇s work in this field. Our and others＇ investigations indicate that the THz spectroscopy and imaging technique can be useful for diagnosing brain tumors and neurodegenerative diseases. THz spectroscopy and imaging has the potential to become real-time in vivo diagnostic methods.展开更多
Bacterial cellulose(BC)is one of the most versatile natural biopolymers with unique physical,chemical,and biological features.However,the lack of intrinsic antibacterial property of native BC limits its broad biomedic...Bacterial cellulose(BC)is one of the most versatile natural biopolymers with unique physical,chemical,and biological features.However,the lack of intrinsic antibacterial property of native BC limits its broad biomedical applications where such property is highly required to prevent contamination or infection caused by attached bacteria.In this work,we developed a simple and facile method to fabricate a dualfunctional BC membrane by physical incorporation of gold nanorods(GNRs)followed by deposition of a phase-transitioned bovine serum albumin(PTB)film.Due to the broad-spectrum antifouling property of the PTB film,the resulting membrane could prevent the adhesion and accumulation of bacteria.A few bacteria that broke through the protection of the PTB film could be eradicated under short-term irradiation of a near-infrared laser due to the excellent photothermal property of incorporated GNRs.The whole fabrication was conducted in a simple and environmentally friendly manner,avoiding complicated processes and toxic organic solvents.Moreover,because all the components were biocompatible,the resulting membrane showed negligible cytotoxicity in vitro and good histocompatibility in vivo.This work thus provided a reliable way to endow BC with antibacterial property,being beneficial for diverse biomedical applications.展开更多
BACKGROUND Sedation during endoscopic ultrasonography(EUS)poses many challenges and moderate-to-deep sedation are often required.The conventional method to preform moderate-to-deep sedation is generally intravenous be...BACKGROUND Sedation during endoscopic ultrasonography(EUS)poses many challenges and moderate-to-deep sedation are often required.The conventional method to preform moderate-to-deep sedation is generally intravenous benzodiazepine alone or in combination with opioids.However,this combination has some limitations.Intranasal medication delivery may be an alternative to this sedation regimen.AIM To determine,by continual reassessment method(CRM),the minimal effective dose of intranasal sufentanil(SUF)when combined with intranasal dexmedetomidine(DEX)for moderate sedation of EUS in at least 95%of patients(ED95).METHODS Thirty patients aged 18-65 and scheduled for EUS were recruited in this study.Subjects received intranasal DEX and SUF for sedation.The dose of DEX(1μg/kg)was fixed,while the dose of SUF was assigned sequentially to the subjects using CRM to determine ED95.The sedation status was assessed by modified observer’s assessment of alertness/sedation(MOAA/S)score.The adverse events and the satisfaction scores of patients and endoscopists were recorded.RESULTS The ED95 was intranasal 0.3μg/kg SUF when combined with intranasal 1μg/kg DEX,with an estimated probability of successful moderate sedation for EUS of 94.9%(95%confidence interval:88.1%-98.9%).When combined with intranasal 1μg/kg DEX,probabilities of successful moderate sedation at each dose level of intranasal SUF were as follows:0μg/kg SUF,52.8%;0.1μg/kg SUF,75.4%;0.2μg/kg SUF,89.9%;0.3μg/kg SUF,94.9%;0.4μg/kg SUF,98.0%;0.5μg/kg SUF,99.0%.CONCLUSION The ED95 needed for moderate sedation for EUS is intranasal 0.3μg/kg SUF when combined with intranasal 1μg/kg DEX,based on CRM.展开更多
Some physicochemical characteristics of calamondin(Citrus microcarpa)from Hainan were determined in this study.The mean length,width,and thickness of calamondin were(2.763±0.262),(2.721±0.189),and(2.684±...Some physicochemical characteristics of calamondin(Citrus microcarpa)from Hainan were determined in this study.The mean length,width,and thickness of calamondin were(2.763±0.262),(2.721±0.189),and(2.684±0.201)cm,respectively.The average values for geometric mean diameter,sphericity,fruit shape index,mass,surface area,volume,true density,bulk density and porosity were(2.721±0.196)cm,(98.79±5.149)%,(101.64±7.609)%,(11.18±2.149)g,(23.36±3.362)cm2,(11.16±2.115)cm3,(1.00±0.060)g/cm3,0.44 g/cm3,and(56.40±0.027)%,respectively.The mean edible part,juice rate,and soluble solids were(85.77±3.53),(48.36±4.20),and(7.86±0.09)%,respectively.The total flavonoids,pectin,limonin,reducing sugar and volatile oil contents of the peel were 1.00,7.14,0.51,5.98,and 4.25%,respectively.The total flavonoids,pectin,limonin and reducing sugar contents of the pulp were 0.02,5.04,0.03 and 4.56%,respectively.The total flavonoids,total acid,reducing sugar and vitamin E contents of the juice were 0.11,6.74,0.37%,and 1.68 mg/kg,respectively,while the content of vitamins A,B1,B2,B3,B6,and D in the juice was very low.The seeds had limonin,reducing sugar and oil contents of 0.46,1.39,and 30.46%,respectively.The vitamin B2,B3,B6 and E contents of the fruit residue were 44.83,19.31,17.55,and 15.72 mg/kg,respectively.The amino acid profile of the residue included aspartic acid,glutamate,cystine,serine,glycine,histidine,arginine,threonine,alanine,proline,tyrosine,valine,methionine,isoleucine,leucine,phenylalanine and lysine,with respective contents of 4.42,1.81,2.10,0.78,0.59,0.20,0.72,0.160.67,3.650.50,0.67,0.59,1.00,0.76,0.57 and 0.91 g/kg.展开更多
基金supported by the National Natural Science Foundation of China(No.22175125)the Natural Science Foundation of the Jiangsu Higher Education Institutions of China(No.21KJA150008)the Key Laboratory of Polymeric Materials De-sign and Synthesis for Biomedical Function,Soochow University,and the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD).
文摘Bacterial biofilms,especially those caused by multidrug-resistant bacteria,have emerged as one of the greatest dangers to global public health.The acceleration of antimicrobial resistance to conventional an-tibiotics and the severe lack of new drugs necessitates the development of novel agents for biofilm eradication.Photodynamic therapy(PDT)is a promising non-antibiotic method for treating bacterial infections.However,its application in biofilm eradication is hampered by the hypoxic microenvironment of biofilms and the physical protection of extracellular polymeric substances.In this study,we develop a composite nanoplatform with oxygen(O_(2))self-supplying and heat-sensitizing capabilities to improve the PDT efficacy against biofilms.CaO_(2)/ICG@PDA nanoparticles(CIP NPs)are fabricated by combining calcium peroxide(CaO_(2))with the photosensitizer indocyanine green(ICG)via electrostatic interactions,followed by coating with polydopamine(PDA).The CIP NPs can gradually generate O_(2)in response to the acidic microenvironment of the biofilm,thereby alleviating its hypoxic state.Under near-infrared(NIR)irradiation,the nanoplatform converts O_(2)into a significant amount of singlet oxygen(^(1)O_(2))and heat to eradicate biofilm.The generated heat enhances the release of O_(2),accelerates the generation of^(1)O_(2)in PDT,increases cell membrane permeability,and increases bacterial sensitivity to^(1)O_(2).This nanoplatform significantly improves the efficacy of PDT in eradicating biofilm-dwelling bacteria without fostering drug resistance.Experiments on biofilm eradication demonstrate that this nanoplatform can eradicate over 99.9999%of methicillin-resistant Staphylococcus aureus(MRSA)biofilms under 5-min NIR irradiation.Notably,these integrated advantages enable the system to promote the healing of MRSA biofilm-infected wounds with negligible toxicity in vivo,indicating great promise for overcoming the obstacles associated with bacterial biofilm eradication.
基金supported by grants from the National Natural Science Foundation of China(32170238,32400191)Guangdong Basic and Applied Basic Research Foundation(2023A1515111029)+2 种基金the Science,Technology and Innovation Commission of Shenzhen Municipality(RCYX20200714114538196)the Chinese Academy of Agricultural Sciences Elite Youth Program(grant 110243160001007)the Guangdong Pearl River Talent Program(2021QN02N792)。
文摘Single-stranded DNA-binding proteins(SSBs)play essential roles in the replication,recombination and repair processes of organellar DNA molecules.In Arabidopsis thaliana,SSBs are encoded by a small family of two genes(SSB1 and SSB2).However,the functional divergence of these two SSB copies in plants remains largely unknown,and detailed studies regarding their roles in the replication and recombination of organellar genomes are still incomplete.In this study,phylogenetic,gene structure and protein motif analyses all suggested that SSB1 and SSB2 probably diverged during the early evolution of seed plants.Based on accurate long-read sequencing results,ssb1 and ssb2 mutants had decreased copy numbers for both mitochondrial DNA(mtDNA)and plastid DNA(ptDNA),accompanied by a slight increase in structural rearrangements mediated by intermediate-sized repeats in mt genome and small-scale variants in both genomes.Our findings provide an important foundation for further investigating the effects of DNA dosage in the regulation of mutation frequencies in plant organellar genomes.
基金financial support from the Natural Science Foundation of Shandong Province of China(ZR2023ME051,ZR2019MEM020)。
文摘Facilitating anion redox chemistry is an effective strategy to increase the capacity of layered oxides for sodium-ion batteries.Nevertheless,there remains a paucity of literature pertaining to the oxygen redox chemistry of O3-type layered oxide cathode materials.This work systematically investigates the effect of Fe doping on the anionic oxygen redox chemistry and electrochemical reactions in O3-NaNi_(0.4)Cu_(0.1)Mn_(0.4)Ti_(0.1)O_(2).The results of the density functional theory(DFT)calculations indicate that the electrons of the O 2p occupy a higher energy level.In the ex-situ X-ray photoelectron spectrometer(XPS)of O 1s,the addition of Fe facilitates the lattice oxygen(O^(n-))to exhibit enhanced activity at 4.45 V.The in-situ X-ray diffraction(XRD)demonstrates that the doping of Fe effectively suppresses the Y phase transition at high voltages.Furthermore,the Galvanostatic Intermittent Titration Technique(GITT)data indicate that Fe doping significantly increases the Na~+migration rate at high voltages.Consequently,the substitution of Fe can elevate the cut-off voltage to 4.45 V,thereby facilitating electron migration from O^(2-).The redox of O^(2-)/O^(n-)(n<2)contributes to the overall capacity.O3-Na(Ni_(0.4)Cu_(0.1)Mn_(0.4)Ti_(0.1))_(0.92)Fe_(0.08)O_(2)provides an initial discharge specific capacity of 180.55 mA h g^(-1)and71.6%capacity retention at 0.5 C(1 C=240 mA g^(-1)).This work not only demonstrates the beneficial impact of Fe substitution for promoting the redox activity and reversibility of O^(2-)in 03-type layered oxides,but also guarantees the structural integrity of the cathode materials at high voltages(>4.2 V).It offers a novel avenue for investigating the anionic redox reaction in O3-type layered oxides to design advanced cathode materials.
基金supported by the National Natural Science Foundation of China(No.22175125)the Natural Science Foundation of the Jiangsu Higher Education Institutions of China(No.21KJA150008)+2 种基金the Natural Science Foundation of Jiangsu Province(No.BK20231314)the Key Laboratory of Polymeric Materials Design and Synthesis for Biomedical Function,Soochow Universitythe Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD).
文摘Due to the absence of timely and effective therapies,infections induced by bacterial biofilms have been widely acknowledged as a significant global public health concern.In modern times,aside from surgical intervention(when appropriate),antibiotics are the sole clinical option for treating biofilm-associated infections.However,the rise of drug resistance,as well as the poor therapeutic effects of current treatment regimens in eliminating biofilms highlight the requirement for novel strategies to enhance the accessibility of antibiotics in the"post-antibiotic era".The current study presents a multifunctional nanoplatform equipped with a"disruption and killing"function to enhance the effectiveness of conventional antibiotics for the eradication of biofilms.Herein,mesoporous silica nanoparticles were employed as carriers to encapsulate the model antibiotic rifampicin(Rif).Subsequently,the nanoparticles were coated with layers of the tannic acid/iron ion(TA/Fe)complex and immobilized with α-amylase.The α-amylase present in the outer layer can degrade the polysaccharides of extracellular polymeric substances(EPS),which in turn disrupts the structural integrity of the biofilms,thus facilitating the entry of the nanoplatform.When exposed to near-infrared(NIR)light,the TA/Fe complex layers can generate heat,which facilitates the release of Rif and increases the bacterial uptake of Rif by damaging the bacterial cell membrane,ultimately resulting in the elimination of bacteria within biofilms.The in vitro experiments demonstrated that this nanoplatform effectively eliminated over 99% of biofilms formed by Staphylococcus aureus and Pseudomonas aeruginosa when exposed to NIR radiation for 10 min.Additionally,in vivo experimental findings further validated the extensive therapeutic efficacy of this nanoplatform against biofilm-infected wounds,accelerating the rate of healing and reducing inflammatory reactions.To summarize,this nanoplatform provides a novel avenue to improve the effectiveness of conventional antibiotics in eradicating bacterial biofilms.
基金National Natural Science Foundation of China(Nos.82330075 and 81401047)Postdoctoral Fellowship Program of China Postdoctoral Science Foundation(GZC20241275)。
文摘Osteoarthritis(OA),the most prevalent degenerative joint disease,is marked by cartilage degradation and pathological alterations in surrounding tissues.Currently,no effective disease-modifying treatments exist.This study aimed to elucidate the critical roles of Myb-like,SWIRM,and MPN domains 1(MYSM1)and its downstream effector,Receptor-interacting protein kinase 2(RIPK2),in OA pathogenesis and the underlying mechanisms.Our findings revealed reduced MYSM1 levels in the cartilage of OA patients and mouse models.Genetic or adenovirus-induced MYSM1 knockout exacerbated OA progression in mice,whereas MYSM1 overexpression mitigated it.Mechanistically,MYSM1 inhibited the NF-κB and MAPK signaling pathways.Conversely,downstream RIPK2 significantly increased OA-like phenotypes and activated the NF-κB and MAPK pathways.The Ripk2^(S176D) mutation accelerated OA pathogenesis,while Ripk2 silencing or Ripk2^(S176A)mutation deactivated NF-κB and MAPK pathways,counteracting the role of MYSM1.MYSM1 deubiquitinates and dephosphorylates RIPK2^(S176)by recruiting protein phosphatase 2 A(PP2A).These results suggest that targeting MYSM1 or downstream RIPK2 offers promising therapeutic potential for OA.
基金Supported by“Renfu”Research Fund by Chinese Society of Digestive Endoscopy,No.CSDE012017120006.
文摘BACKGROUND Hypoxemia due to respiratory depression and airway obstruction during upper gastrointestinal endoscopy with sedation is a common concern.The Wei nasal jet tube(WNJT)is a new nasopharyngeal airway with the ability to provide supraglottic jet ventilation and oxygen insufflation via its built-in wall channel.The available evidence indicates that with a low oxygen flow,compared with nasal cannula,the WNJT does not decrease the occurrence of hypoxemia during upper gastrointestinal endoscopy with propofol sedation.To date,there has been no study assessing the performance of WNJT for supplemental oxygen during upper gastrointestinal endoscopy with sedation when a moderate oxygen flow is used.AIM To determine whether the WNJT performs better than the nasal prongs for the prevention of hypoxemia during gastroscopy with propofol mono-sedation when a moderate oxygen flow is provided in patients with a normal body mass index.METHODS This study was performed in 291 patients undergoing elective gastroscopy with propofol mono-sedation.Patients were randomized into one of two groups to receive either the WNJT(WNJT group,n=147)or the nasal cannula(nasal cannula group,n=144)for supplemental oxygen at a 5-L/min flow during gastroscopy.The lowest SpO2 during gastroscopy was recorded.The primary endpoint was the incidence of hypoxemia or severe hypoxemia during gastroscopy.RESULTS The total incidence of hypoxemia and severe hypoxemia during gastroscopy was significantly decreased in the WNJT group compared with the nasal cannula group(P=0.000).The lowest median SpO2 during gastroscopy was significantly higher(98%;interquartile range,97-99)in the WNJT group than in the nasal cannula group(96%;interquartile range,93-98).Epistaxis by device insertion in the WNJT group occurred in 7 patients but stopped naturally without any treatment.The two groups were comparable in terms of the satisfaction of physicians,anesthetists and patients.CONCLUSION With a moderate oxygen flow,the WNJT is more effective for the prevention of hypoxemia during gastroscopy with propofol mono-sedation compared with nasal prongs,but causing slight epistaxis in a few patients.
基金financially supported by the National Natural Science Foundation of China(Nos.21774086 and 21935008)the Natural Science Foundation of Jiangsu Province(No.BK20180093)+1 种基金the Suzhou Municipal Science and Technology Foundation(No.SYS2018026)the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)。
文摘Attachment of bacteria and subsequent formation of biofilms on material surfaces lead to serious consequences including infection,contamination and biofouling,posing a prominent threat to human health and causing problems in many industries.Therefore,it is highly desirable to endow the surfaces with antibacterial properties.Traditional antibacterial surfaces are designed via either bacteria-resisting strategy to prevent the initial adhesion of bacteria or bacteria-killing strategy to eradicate any bacteria that attach to the surface.However,these single-function surfaces have their inherent shortcomings and cannot realize long-term efficacy against bacteria.In recent years,various dual-function antibacterial surfaces with both bacteria-resisting and bacteria-killing properties together have been developed,showing better performance for combating surface-attached bacteria and preventing formation of biofilms.In this review,we summarize the recent development of these dual-function antibacterial surfaces.We focus on the design principles and fabrication strategies of such surfaces and highlight the representative examples,which are categorized specifically into two types according to the anti-adhesive and bactericidal properties are simultaneous or switchable.A brief perspective is finally presented on current challenges and future research directions.
基金funded by the National High Technology Research and Development Program of China(863 Program),No.2008AA022400a grant from Science&Technology Department of Sichuan Province of China,No.2009JY0174
文摘Phrenic nerve transfer is a major dynamic treatment used to repair brachial plexus root avulsion. We analyzed 72 relevant articles on phrenic nerve transfer to repair injured brachial plexus that were indexed by Science Citation Index. The keywords searched were brachial plexus injury, phrenic nerve, repair, surgery, protection, nerve transfer, and nerve graft. In addition, we per-formed neurophysiological analysis of the preoperative condition and prognosis of 10 patients undergoing ipsilateral phrenic nerve transfer to the musculocutaneous nerve in our hospital from 2008 to 201 3 and observed the electromyograms of the biceps brachii and motor conduc-tion function of the musculocutaneous nerve. Clinically, approximately 28% of patients had brachial plexus injury combined with phrenic nerve injury, and injured phrenic nerve cannot be used as a nerve graft. After phrenic nerve transfer to the musculocutaneous nerve, the regener-ated potentials ifrst appeared at 3 months. Recovery of motor unit action potential occurred 6 months later and became more apparent at 12 months. The percent of patients recovering ‘ex-cellent’ and ‘good’ muscle strength in the biceps brachii was 80% after 18 months. At 12 months after surgery, motor nerve conduction potential appeared in the musculocutaneous nerve in seven cases. These data suggest that preoperative evaluation of phrenic nerve function may help identify the most appropriate nerve graft in patients with an injured brachial plexus. The func-tional recovery of a transplanted nerve can be dynamically observed after the surgery.
基金supported in part by National Natural Science Foundation of China under Grants No. 61871404, 61401510, 61521003, 61501516
文摘In this paper, we propose a dual-threshold based secure On-Off transmission scheme, where signals are transmitted only if the channel condition can guarantee secure and reliable communication. First, we present a dynamic access strategy to increase access efficiency, which provides an access region for the intended user. Then, we propose an emission control policy to transmit signals according to the current channel condition, which declines the influence of channel estimation errors and guarantees qualities of communication links. Furthermore, we give a comprehensive performance analysis for the proposed scheme in terms of connection outage probability(COP) and secrecy outage probability(SOP), and present a dual-threshold optimization model to further support the performance. Numerical results verify that the secure On-Off transmission scheme can increase the system secure energy efficiency and guarantee reliable and secure communication.
基金the National Natural Science Foundation of China(No.22175125)the Natural Science Foundation of the Jiangsu Higher Education Institutions of China(No.21KJA150008)the Key Laboratory of Polymeric Materials De-sign and Synthesis for Biomedical Function,Soochow University,and the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD).
文摘For most traditional wound dressings,it is challenging to simultaneously eliminate bacteria and promote angiogenesis to accelerate the healing process of bacteria-infected wounds.In this work,we develop a multifunctional dressing based on bacterial cellulose(BC)deposited with a tannic acid/Cu^(2+)ion/Mg^(2+)ion(TCM)complex film.Overall,the TCM complex exhibits robust interfacial adhesion to modify BC and good photothermal properties to effectively eradicate bacteria in the wound area under near-infrared(NIR)irradiation.The individual components of the TCM complex have several advantageous features for wound healing,such as antibacterial ability and negligible cytotoxicity;in particular,the released Cu^(2+)and Mg^(2+)ions are favorable for the proliferation,migration,and tube formation of endothelial cells in vitro.The results of in vivo experiments demonstrated that with the assistance of NIR irradiation,this composite dressing is more effective than traditional gauze or pristine BC dressing in promotion of angiogenesis and collagen deposition without causing remarkable inflammation,thereby accelerating the healing process of bacteria-infected full-thickness skin wounds.This work thus provides a simple and facile way to fabricate multifunctional BC-based dressings that could be potentially used for treating infected wounds.
基金supported by the National Natural Science Foundation of China(Nos.22175125 and 21774086)the Natural Science Foundation of the Jiangsu Higher Education Insti-tutions of China(No.21KJA150008)the Priority Academic Pro-gram Development of Jiangsu Higher Education Institutions(PAPD).
文摘Bacterial biofilms formed on the material surfaces have posed a series of serious problems for human health and industries.The treatment of mature biofilms is particularly difficult because they are inher-ently highly resistant against antibiotics and other adverse factors.The prevention is strategically advan-tageous over the treatment,and thus the development of innovative surfaces with capability to inhibit biofilm formation is highly demanded.In this work,we developed a superhydrophobic photothermal coating for prevention of biofilm formation,which was based on candle soot with hierarchical structure and excellent light-to-heat conversion ability.This coating was fabricated by deposition of a candle soot layer on the substrate,followed by sequential chemical vapor deposition of tetraethoxysilane and immo-bilization of fluorinated silane to make the coating robust and superhydrophobic.The resulted coating could repel a majority of bacteria from the surface at the early stage,and then eradicate a small number of bacteria remained on the surface under a short-term irradiation of near-infrared laser.The combi-nation of anti-adhesive property and photothermal bactericidal property endowed the coating with good antibiofilm property to prevent biofilm formation for at least 2 weeks.This coating is facile for deposition on various substrates with good storage stability,showing great potential for diverse practical applications to solve the biofilm-associated problems of materials and devices.
基金supported by the State Key Development Program for Basic Research of China under Grant No.2015CB755405National Natural Science Foundation of China under Grant No.61427814+1 种基金Joint Fund of the National Natural Science Foundation of China and the China Academy of Engineering Physics under Grant No.U1230128China Postdoctoral Science Foundation under Grant No.2014M552542XB and No.2015T80989
文摘Brain is the most complex organ of human beings. The complete understanding of human brains＇ functions is an ongoing challenge for neuroscience. Although there are many techniques used for brain research, neuroscientists, physicists, and engineers are still exploring other access of research for applications. Terahertz （THz） spectroscopy and imaging may create such a space for brain research. THz spectroscopic technique, which is a novel medical imaging modality, attracts attention of biomedical applications due to its unique features including non-invasive, non-ionizing, and high sensitivity to biomolecules and tissues. In this article, we introduce our recent investigations on neuroscience using THz techniques, meanwhile, introduce other＇s work in this field. Our and others＇ investigations indicate that the THz spectroscopy and imaging technique can be useful for diagnosing brain tumors and neurodegenerative diseases. THz spectroscopy and imaging has the potential to become real-time in vivo diagnostic methods.
基金financially supported by the National Natural Science Foundation of China(Nos.21774086 and 22175125)the Natural Science Foundation of Jiangsu Province(No.BK20180093)+1 种基金the Natural Science Foundation of the Jiangsu Higher Education Institutions of China(No.21KJA150008)the Suzhou Municipal Science and Technology Foundation(No.SYS2018026)。
文摘Bacterial cellulose(BC)is one of the most versatile natural biopolymers with unique physical,chemical,and biological features.However,the lack of intrinsic antibacterial property of native BC limits its broad biomedical applications where such property is highly required to prevent contamination or infection caused by attached bacteria.In this work,we developed a simple and facile method to fabricate a dualfunctional BC membrane by physical incorporation of gold nanorods(GNRs)followed by deposition of a phase-transitioned bovine serum albumin(PTB)film.Due to the broad-spectrum antifouling property of the PTB film,the resulting membrane could prevent the adhesion and accumulation of bacteria.A few bacteria that broke through the protection of the PTB film could be eradicated under short-term irradiation of a near-infrared laser due to the excellent photothermal property of incorporated GNRs.The whole fabrication was conducted in a simple and environmentally friendly manner,avoiding complicated processes and toxic organic solvents.Moreover,because all the components were biocompatible,the resulting membrane showed negligible cytotoxicity in vitro and good histocompatibility in vivo.This work thus provided a reliable way to endow BC with antibacterial property,being beneficial for diverse biomedical applications.
基金Supported by the Research Foundation of Beijing Friendship Hospital,Capital Medical University,No. yyqdkt2018-16the Beijing Municipal Administration of Hospitals’ Youth Program,No. QML20190101the Scientific Research Common Program of Beijing Municipal Commission of Education,No. KM202010025021
文摘BACKGROUND Sedation during endoscopic ultrasonography(EUS)poses many challenges and moderate-to-deep sedation are often required.The conventional method to preform moderate-to-deep sedation is generally intravenous benzodiazepine alone or in combination with opioids.However,this combination has some limitations.Intranasal medication delivery may be an alternative to this sedation regimen.AIM To determine,by continual reassessment method(CRM),the minimal effective dose of intranasal sufentanil(SUF)when combined with intranasal dexmedetomidine(DEX)for moderate sedation of EUS in at least 95%of patients(ED95).METHODS Thirty patients aged 18-65 and scheduled for EUS were recruited in this study.Subjects received intranasal DEX and SUF for sedation.The dose of DEX(1μg/kg)was fixed,while the dose of SUF was assigned sequentially to the subjects using CRM to determine ED95.The sedation status was assessed by modified observer’s assessment of alertness/sedation(MOAA/S)score.The adverse events and the satisfaction scores of patients and endoscopists were recorded.RESULTS The ED95 was intranasal 0.3μg/kg SUF when combined with intranasal 1μg/kg DEX,with an estimated probability of successful moderate sedation for EUS of 94.9%(95%confidence interval:88.1%-98.9%).When combined with intranasal 1μg/kg DEX,probabilities of successful moderate sedation at each dose level of intranasal SUF were as follows:0μg/kg SUF,52.8%;0.1μg/kg SUF,75.4%;0.2μg/kg SUF,89.9%;0.3μg/kg SUF,94.9%;0.4μg/kg SUF,98.0%;0.5μg/kg SUF,99.0%.CONCLUSION The ED95 needed for moderate sedation for EUS is intranasal 0.3μg/kg SUF when combined with intranasal 1μg/kg DEX,based on CRM.
基金Technical Development Project of Provincial Scientific Research Institutes in Hainan(KYYS-2019-30)Technical Innovation Project of Provincial Scientific Research Institutes in Hainan(jscx202034).
文摘Some physicochemical characteristics of calamondin(Citrus microcarpa)from Hainan were determined in this study.The mean length,width,and thickness of calamondin were(2.763±0.262),(2.721±0.189),and(2.684±0.201)cm,respectively.The average values for geometric mean diameter,sphericity,fruit shape index,mass,surface area,volume,true density,bulk density and porosity were(2.721±0.196)cm,(98.79±5.149)%,(101.64±7.609)%,(11.18±2.149)g,(23.36±3.362)cm2,(11.16±2.115)cm3,(1.00±0.060)g/cm3,0.44 g/cm3,and(56.40±0.027)%,respectively.The mean edible part,juice rate,and soluble solids were(85.77±3.53),(48.36±4.20),and(7.86±0.09)%,respectively.The total flavonoids,pectin,limonin,reducing sugar and volatile oil contents of the peel were 1.00,7.14,0.51,5.98,and 4.25%,respectively.The total flavonoids,pectin,limonin and reducing sugar contents of the pulp were 0.02,5.04,0.03 and 4.56%,respectively.The total flavonoids,total acid,reducing sugar and vitamin E contents of the juice were 0.11,6.74,0.37%,and 1.68 mg/kg,respectively,while the content of vitamins A,B1,B2,B3,B6,and D in the juice was very low.The seeds had limonin,reducing sugar and oil contents of 0.46,1.39,and 30.46%,respectively.The vitamin B2,B3,B6 and E contents of the fruit residue were 44.83,19.31,17.55,and 15.72 mg/kg,respectively.The amino acid profile of the residue included aspartic acid,glutamate,cystine,serine,glycine,histidine,arginine,threonine,alanine,proline,tyrosine,valine,methionine,isoleucine,leucine,phenylalanine and lysine,with respective contents of 4.42,1.81,2.10,0.78,0.59,0.20,0.72,0.160.67,3.650.50,0.67,0.59,1.00,0.76,0.57 and 0.91 g/kg.
