目的探讨针刺对高脂饮食诱导的动脉粥样硬化(AS)兔脂质代谢和肠道菌群的影响及其作用机制。方法24只新西兰兔随机分为空白组、模型组与针刺组,每组8只。空白组正常饲料喂养;模型组高脂饲料喂养;针刺组高脂饲料喂养并针刺干预,每天干预1...目的探讨针刺对高脂饮食诱导的动脉粥样硬化(AS)兔脂质代谢和肠道菌群的影响及其作用机制。方法24只新西兰兔随机分为空白组、模型组与针刺组,每组8只。空白组正常饲料喂养;模型组高脂饲料喂养;针刺组高脂饲料喂养并针刺干预,每天干预1次,连续12周。全自动生化仪检测血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)以及高密度脂蛋白胆固醇(HDL-C)含量;HE染色和油红O染色观察兔主动脉病理变化及斑块面积;16S rDNA测序分析肠道菌群结构及功能。结果与空白组比较,模型组TC、TG、LDL-C含量均升高(P<0.01),HDL-C含量降低(P<0.01);胸主动脉斑块面积比升高(P<0.05)。与模型组比较,针刺组TC、TG、LDL-C含量均下降(P<0.01),HDL-C含量升高(P<0.05);胸主动脉斑块面积比降低(P<0.05)。16S rDNA测序结果表明,针刺可调节AS兔的肠道菌群结构,并影响菌群α和β多样性。与空白组比较,模型组厚壁菌门(Firmicutes)、NK4A214_group菌属相对丰度升高(P<0.05);拟杆菌门(Bacteroidota)、蓝藻菌门(Cyanobacteria)、穆里杆菌属(Muribaculacea)、拟杆菌属(Bacteroides)相对丰度降低(P<0.05)。与模型组比较,针刺组拟杆菌门、蓝藻菌门、穆里杆菌属、拟杆菌属相对丰度升高(P<0.05);NK4A214_group菌属相对丰度下降(P<0.05)。KEGG功能预测显示,与空白组比较,模型组其他次级代谢产物的生物合成(Biosynthesis of other secondary metabolites)和糖链生物合成与代谢(Glycan biosynthesis and metabolism)相对丰度均降低(P<0.05)。与模型组比较,针刺组糖链生物合成与代谢相对丰度升高(P<0.05)。结论针刺可能通过调节肠道菌群多样性和结构,改善AS兔脂质代谢及主动脉损伤;同时通过影响糖链生物合成与代谢功能,促进内皮功能恢复,从而发挥抗AS作用。展开更多
Objective:To investigate whether herb cake-insulated moxibustion affects the expression of cholesterol metabolismrelated protein 3-hydroxy-3-methylglutaryl coenzyme A reductase(HMGR)and inhibits the development of ath...Objective:To investigate whether herb cake-insulated moxibustion affects the expression of cholesterol metabolismrelated protein 3-hydroxy-3-methylglutaryl coenzyme A reductase(HMGR)and inhibits the development of atherosclerosis by regulating the exosomal miR-223 expression.Methods:Thirty-six male New Zealand rabbits were randomly assigned to a normal group,a model group,and an herb cake-insulated moxibustion group,with 12 rabbits in each group.The model and the herb cake-insulated moxibustion groups were fed a high-fat diet for 8 weeks to induce an atherosclerosis model.Following successful modeling,the herb cake-insulated moxibustion group was subjected to bundling and herb cake-insulated moxibustion intervention,while the other two groups were subjected only to bundling without moxibustion.After 8 weeks of intervention,hematoxylin-eosin(HE)staining was used to observe aortic morphology;the serum levels of total cholesterol(TC),triglyceride(TG),and low-density lipoprotein cholesterol(LDL-C)were detected using an automatic biochemical analyzer in each group.Exosome morphology was observed using the transmission electron microscope;Western blotting(WB)was used to detect the protein levels of serum exosomal CD63 and CD9 markers,as well as liver HMGR;additionally,real-time fluorescence quantitative polymerase chain reaction was used to quantify serum exosomal miR-223.Results:HE staining showed thickened aortic intima,lipid infiltration,foam cell aggregation,and structural damage to the arterial wall in the model group.Meanwhile,after modeling,the serum levels of LDL-C,TC,and TG increased significantly in the model and herb cake-insulated moxibustion groups compared to the normal group(P<0.05),suggesting successful atherosclerosis rabbit model preparation.The serum exosomes of rabbits in the model group exhibited a saucer-like or semi-concave spherical shape with diameters of 120-150 nm.WB detection results showed positive expression of the exosomal markers CD63 and CD9.After 8 weeks of intervention,the miR-223 level in the model group was significantly lower than that in the normal group(P<0.01).In contrast,the herb cake-insulated moxibustion group demonstrated significantly reduced serum levels of TC,TG,and LDL-C(P<0.05),increased miR-223 expression(P<0.01),and decreased relative liver HMGR protein expression(P<0.05)compared to the model group.Conclusion:Herb cake-insulated moxibustion may alleviate the progression of atherosclerosis by up-regulating exosomal miR-223 expression and down-regulating HMGR protein expression,thereby inhibiting cholesterol anabolic metabolism.展开更多
文摘目的探讨针刺对高脂饮食诱导的动脉粥样硬化(AS)兔脂质代谢和肠道菌群的影响及其作用机制。方法24只新西兰兔随机分为空白组、模型组与针刺组,每组8只。空白组正常饲料喂养;模型组高脂饲料喂养;针刺组高脂饲料喂养并针刺干预,每天干预1次,连续12周。全自动生化仪检测血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)以及高密度脂蛋白胆固醇(HDL-C)含量;HE染色和油红O染色观察兔主动脉病理变化及斑块面积;16S rDNA测序分析肠道菌群结构及功能。结果与空白组比较,模型组TC、TG、LDL-C含量均升高(P<0.01),HDL-C含量降低(P<0.01);胸主动脉斑块面积比升高(P<0.05)。与模型组比较,针刺组TC、TG、LDL-C含量均下降(P<0.01),HDL-C含量升高(P<0.05);胸主动脉斑块面积比降低(P<0.05)。16S rDNA测序结果表明,针刺可调节AS兔的肠道菌群结构,并影响菌群α和β多样性。与空白组比较,模型组厚壁菌门(Firmicutes)、NK4A214_group菌属相对丰度升高(P<0.05);拟杆菌门(Bacteroidota)、蓝藻菌门(Cyanobacteria)、穆里杆菌属(Muribaculacea)、拟杆菌属(Bacteroides)相对丰度降低(P<0.05)。与模型组比较,针刺组拟杆菌门、蓝藻菌门、穆里杆菌属、拟杆菌属相对丰度升高(P<0.05);NK4A214_group菌属相对丰度下降(P<0.05)。KEGG功能预测显示,与空白组比较,模型组其他次级代谢产物的生物合成(Biosynthesis of other secondary metabolites)和糖链生物合成与代谢(Glycan biosynthesis and metabolism)相对丰度均降低(P<0.05)。与模型组比较,针刺组糖链生物合成与代谢相对丰度升高(P<0.05)。结论针刺可能通过调节肠道菌群多样性和结构,改善AS兔脂质代谢及主动脉损伤;同时通过影响糖链生物合成与代谢功能,促进内皮功能恢复,从而发挥抗AS作用。
文摘Objective:To investigate whether herb cake-insulated moxibustion affects the expression of cholesterol metabolismrelated protein 3-hydroxy-3-methylglutaryl coenzyme A reductase(HMGR)and inhibits the development of atherosclerosis by regulating the exosomal miR-223 expression.Methods:Thirty-six male New Zealand rabbits were randomly assigned to a normal group,a model group,and an herb cake-insulated moxibustion group,with 12 rabbits in each group.The model and the herb cake-insulated moxibustion groups were fed a high-fat diet for 8 weeks to induce an atherosclerosis model.Following successful modeling,the herb cake-insulated moxibustion group was subjected to bundling and herb cake-insulated moxibustion intervention,while the other two groups were subjected only to bundling without moxibustion.After 8 weeks of intervention,hematoxylin-eosin(HE)staining was used to observe aortic morphology;the serum levels of total cholesterol(TC),triglyceride(TG),and low-density lipoprotein cholesterol(LDL-C)were detected using an automatic biochemical analyzer in each group.Exosome morphology was observed using the transmission electron microscope;Western blotting(WB)was used to detect the protein levels of serum exosomal CD63 and CD9 markers,as well as liver HMGR;additionally,real-time fluorescence quantitative polymerase chain reaction was used to quantify serum exosomal miR-223.Results:HE staining showed thickened aortic intima,lipid infiltration,foam cell aggregation,and structural damage to the arterial wall in the model group.Meanwhile,after modeling,the serum levels of LDL-C,TC,and TG increased significantly in the model and herb cake-insulated moxibustion groups compared to the normal group(P<0.05),suggesting successful atherosclerosis rabbit model preparation.The serum exosomes of rabbits in the model group exhibited a saucer-like or semi-concave spherical shape with diameters of 120-150 nm.WB detection results showed positive expression of the exosomal markers CD63 and CD9.After 8 weeks of intervention,the miR-223 level in the model group was significantly lower than that in the normal group(P<0.01).In contrast,the herb cake-insulated moxibustion group demonstrated significantly reduced serum levels of TC,TG,and LDL-C(P<0.05),increased miR-223 expression(P<0.01),and decreased relative liver HMGR protein expression(P<0.05)compared to the model group.Conclusion:Herb cake-insulated moxibustion may alleviate the progression of atherosclerosis by up-regulating exosomal miR-223 expression and down-regulating HMGR protein expression,thereby inhibiting cholesterol anabolic metabolism.
