Plant viruses pose significant threats to agriculture,with many vectored by insect pests.The entry of viruses and their encoded proteins into the host nucleus is a critical step for promoting some viral replication an...Plant viruses pose significant threats to agriculture,with many vectored by insect pests.The entry of viruses and their encoded proteins into the host nucleus is a critical step for promoting some viral replication and enabling systemic infection.Laodelphax striatellus,also known as the small brown planthopper(SBPH),is an efficient vector for rice stripe virus(RSV),one of the most damaging viruses of rice.In this study,we demonstrate that RSV infection induces the expression of genes in both the classical and non-classical nuclear import pathways of SBPH.A gene belonging to the importinβfamily,importin 5(LsIPO5),was upregulated by 84%in SBPH midguts infected with RSV.The nuclear localization signal(NLS,^(168)YRSPSKKRHKYV^(179))is located within the nonstructural protein NS3 directly bound to LsIPO5,thereby facilitating NS3nuclear entry.Moreover,a RING-type E3 ligase(LsRING)in SBPH,which mediated the ubiquitination of NS3 in the insect vector,enhanced NS3 binding to LsIPO5 and facilitated NS3 perinuclear localization.Combined treatment of SBPH with both ds IPO5 and ds RING significantly reduced RSV loads,highlighting the importance of LsIPO5 and NS3 ubiquitination cooperation in facilitating viral replication.Our findings provide new insights into synergistic molecular mechanisms that govern RSV infection and suggest potential therapeutic targets to control viral transmission through their insect vectors.展开更多
The occurrence of difficult arteriovenous fistulas makes the use of vascular access in hemodialysis patients face great challenges.With the widespread application of point-of-care ultrasound,POCUS technology,it provid...The occurrence of difficult arteriovenous fistulas makes the use of vascular access in hemodialysis patients face great challenges.With the widespread application of point-of-care ultrasound,POCUS technology,it provides a new solution for clinical practice.This article reviews the principle,basic process,application status,current clinical challenges and development direction of difficult arteriovenous fistulas cannulation guided by POCUS technology,in order to provide reference for the standardized clinical application and future research direction of this technology.展开更多
With the global push for energy conservation and the rapid development of low-power,flexible and wearable optical displays,the demand for electrochromic technology has surged.Gel polymer electrolytes(GPEs),a crucial c...With the global push for energy conservation and the rapid development of low-power,flexible and wearable optical displays,the demand for electrochromic technology has surged.Gel polymer electrolytes(GPEs),a crucial component of electrochromic devices(ECDs),show great promise in applications.This is attributed to their efficient ion-transport capabilities,excellent mechanical properties and strong adhesion.All of these characteristics are conducive to enhancing the safety of the devices,streamlining the packaging process,significantly improving the electrochromic performance of ECDs and boosting their commercial application potential.This review provides a comprehensive overview of GPEs for ECDs,focusing on their basic designs,functional modifications and practical applications.Firstly,this review outlines the fundamental design of GPEs for ECDs,encompassing key performance index,classification,gelation mechanism and preparation methods.Building on this foundation,it provides an in-depth discussion of functionalized GPEs developed to enhance device performance or expand functionality,including electrochromic,temperature-responsive,photo-responsive and stretchable self-healing GPE.Furthermore,the integration of GPEs into various ECD applications,including smart windows,displays,energy storage devices and wearable electronic,are summarized to highlight the advantages that the design of GPEs brings to the practical application of ECDs.Finally,based on the summary of GPEs employed for ECDs,the challenges and development expectations in this direction were indicated.展开更多
Self-trapping excitons(STEs) emission in metal halides has been a matter of interest, correlating with the strength of electron-phonon coupling in the lattice, which are usually caused by ions with ns~2 electronic str...Self-trapping excitons(STEs) emission in metal halides has been a matter of interest, correlating with the strength of electron-phonon coupling in the lattice, which are usually caused by ions with ns~2 electronic structure. In this work, Sb^(3+)/Te^(4+)ions doped Zn-based halide single crystals(SCs) with two STEs emissions have been synthesized and the possibility of its anti-counterfeiting application was explored.Further, the relationship between the strength of electron-phonon coupling and photoluminescence quantum yields(PLQYs) for STEs in a series of metal halides has been studied. And the semi-empirical range of the Huang-Rhys factors(S) for metal halides with excellent photoluminescence(PL) property has been summarized. This work provides ideas for further research into the relationship between luminescence performance and electron-phonon coupling of metal halides, and also provides a reference for designing the metal halides with high PLQYs.展开更多
Litchi(Litchi chinensis Sonn.),an important fruit tree in tropical and subtropical regions,possesses substantial economic value.The branchand leaf-related traits of litchi have a significant impact on litchi yield and...Litchi(Litchi chinensis Sonn.),an important fruit tree in tropical and subtropical regions,possesses substantial economic value.The branchand leaf-related traits of litchi have a significant impact on litchi yield and quality.However,due to limitations such as the density of the genetic linkage map,there have been few studies on mapping QTLs of branch-and leaf-related traits.In this study,a high-density genetic map was constructed by next-generation sequencing(NGS)using an F_(1) population of 264 progenies,derived from the cross between the cultivars‘Sanyuehong'and‘Ziniangxi'.A total of 2574 high-quality BINs(binomial intervals)were obtained,and a genetic linkage map was constructed with a total length of 1753.3 cM and an average marker distance of 0.68 cM.With the genetic map and the phenotyping of single leaf length(SLL),single leaf width(SLW),leaf shape index(LSI),weight of specific leaf(WSL),petiole length(PL)and compound leaf length(CLL)measured in three seasons,11,9,9,10,9 and 12 QTLs were detected for SLL,SLW,WSL,LSI,PL and CLL traits,respectively.Among these QTLs,five QTLs were consistently detected in two seasons and 12 pleiotropic QTLs were identified for at least two traits.These findings will provide new insights for the gene cloning for branch-and leaf-related traits as well as marker-assisted selection(MAS).展开更多
Fructus Ligustri Lucidi(FLL)refers to the dried mature fruit of Ligustrum lucidum Ait.,a species from the Oleaceae family,widely distributed across East Asia and India.This study aimed to optimize the extraction proce...Fructus Ligustri Lucidi(FLL)refers to the dried mature fruit of Ligustrum lucidum Ait.,a species from the Oleaceae family,widely distributed across East Asia and India.This study aimed to optimize the extraction process for Fructus Ligustri Lucidi essential oil(FLLO)to develop an efficient and practical extraction method.Additionally,the chemical composition of FLLO was analyzed,and its antioxidant,antimicrobial,and cytotoxic activities were evaluated.FLLO was extracted using supercritical CO_(2) extraction,and response surface methodology was applied to optimize the extraction parameters:pressure of 16 MPa,temperature of 40℃,and extraction time of 40 min.The main components of the essential oil were identified through GC-MS analysis.Antioxidant activity was assessed using DPPH and ABTS assays,demonstrating that FLLO exhibited strong antioxidant properties,with a DPPH radical scavenging rate exceeding 80%.In antimicrobial tests,FLLO exhibited significant inhibitory effects on both Gram-positive and Gram-negative bacteria at concentrations greater than 25 mg/mL.Additionally,cytotoxicity assays revealed that FLLO enhanced the proliferation of LO2 cells.In conclusion,FLLO,extracted using supercritical CO_(2),demonstrates excellent antioxidant and antimicrobial properties,as well as favorable cell safety,supporting its potential for further development and application of Ligustrum lucidum.展开更多
Thermal Protection System(TPS)with thick tiles,low thermal conductivity,and a short re-entry stage stands as a critical element within reusable aircraft,whose reliability is related to the function and changes with th...Thermal Protection System(TPS)with thick tiles,low thermal conductivity,and a short re-entry stage stands as a critical element within reusable aircraft,whose reliability is related to the function and changes with their physical properties,external conditions,and degradation.Meanwhile,due to the limitation of testing resources,epistemic uncertainties stemming from the small samples are present in TPS reliability modeling.However,current TPS reliability modeling methods face challenges in characterizing the relationships among reliability and physical properties,external conditions,degradation,and epistemic uncertainties.Therefore,under the framework of belief reliability theory,a TPS reliability model is constructed,which takes into account the physical principle,external conditions,performance degradation,and epistemic uncertainties.A reliability simulation algorithm is proposed to calculate TPS reliability.Through a case study and comparison analysis,the proposed method is validated as more effective than the existing method.Additionally,reliability sensitivity analysis is conducted to identify the sensitive factors of reliability under the condition of small samples,through which suggestions are provided for TPS functional design and improvement.展开更多
The intergranular corrosion behavior of 2050 Al-Li alloy subjected to non-isothermal aging(NIA)treatment with varying pre-deformation amounts was investigated.Results indicate that the resistance to intergranular corr...The intergranular corrosion behavior of 2050 Al-Li alloy subjected to non-isothermal aging(NIA)treatment with varying pre-deformation amounts was investigated.Results indicate that the resistance to intergranular corrosion improves with increasing pre-deformation amouts.However,when the pre-deformation amount reaches 20%,the corrosion resistance deteriorates.Microstructural analyses via transmission electron microscopy(TEM)and electron backscatter diffraction(EBSD)reveal that as pre-deformation amount increases,the fraction of high-angle grain boundaries(HAGBs)decreases,while the proportion of low-angle grain boundaries(LAGBs)increases.This change provides additional nucleation sites for precipitates,leading to a reduction in T1 phase size and an increase in T1 phase density.The finer T1 phases contribute to a lower localized potential difference within the grains,slowering corrosion propagation.Furthermore,during corrosion,preferential dissolution of Li results in Cu enrichment along grain boundaries,which further reduces the intergranular corrosion resistance.展开更多
We present the first theoretical study on heavy-flavor jet angularities(λ_(α)^(κ))in Pb+Pb collisions at√SNN=5.02 Te V.The initial production of heavy-flavor jets was carried out using the POWHEG+PYTHIA8 prescript...We present the first theoretical study on heavy-flavor jet angularities(λ_(α)^(κ))in Pb+Pb collisions at√SNN=5.02 Te V.The initial production of heavy-flavor jets was carried out using the POWHEG+PYTHIA8 prescription.In contrast,the SHELL transport model describes jet evolution in the quark-gluon plasma(QGP).In p+p collisions,we observed narrower angularity distributions for D ^(0)-tagged jets than for inclusive jets,consistent with the ALICE preliminary results.We then demonstrate that jet quenching in the QGP slightly widens the angularity distribution of D ^(0)-tagged jets in Pb+Pb collisions relative to that in p+p collisions for a jet transverse momentum of 10<pch/T,jet<20 Ge V/c.In contrast,the angularity distributions of the inclusive and D ^(0)-tagged jets become narrower in Pb+Pb collisions relative to p+p atpch/T,jet>20 Ge V/c because of the strong influence of selection bias.Additionally,by comparing the average angularities<λ_(α)^(κ)>of the inclusive,D ^(0)-tagged,and B ^(0)-tagged jets with varyingαandκ,we show that the larger the quark mass,the lower the jet’s<λ_(α)^(κ)>values are.As a result of the slenderer initial distribution,we predict that compared with inclusive jets,heavy-flavor jets,especially B~0-tagged ones,will suffer stronger modifications of<λ_(α)^(κ)>in Pb+Pb relative to p+p at 10<pch/T,jet<20 Ge V/c.For a larger jet radius,a more significant broadening of the jet angularities was predicted because of the enhanced contribution of the wide-angle particles.展开更多
基金supported by the Natural Science Foundation of Jiangsu Province,China(BK20240902 and BK20240904)the National Natural Science Foundation of China(32272533)。
文摘Plant viruses pose significant threats to agriculture,with many vectored by insect pests.The entry of viruses and their encoded proteins into the host nucleus is a critical step for promoting some viral replication and enabling systemic infection.Laodelphax striatellus,also known as the small brown planthopper(SBPH),is an efficient vector for rice stripe virus(RSV),one of the most damaging viruses of rice.In this study,we demonstrate that RSV infection induces the expression of genes in both the classical and non-classical nuclear import pathways of SBPH.A gene belonging to the importinβfamily,importin 5(LsIPO5),was upregulated by 84%in SBPH midguts infected with RSV.The nuclear localization signal(NLS,^(168)YRSPSKKRHKYV^(179))is located within the nonstructural protein NS3 directly bound to LsIPO5,thereby facilitating NS3nuclear entry.Moreover,a RING-type E3 ligase(LsRING)in SBPH,which mediated the ubiquitination of NS3 in the insect vector,enhanced NS3 binding to LsIPO5 and facilitated NS3 perinuclear localization.Combined treatment of SBPH with both ds IPO5 and ds RING significantly reduced RSV loads,highlighting the importance of LsIPO5 and NS3 ubiquitination cooperation in facilitating viral replication.Our findings provide new insights into synergistic molecular mechanisms that govern RSV infection and suggest potential therapeutic targets to control viral transmission through their insect vectors.
文摘The occurrence of difficult arteriovenous fistulas makes the use of vascular access in hemodialysis patients face great challenges.With the widespread application of point-of-care ultrasound,POCUS technology,it provides a new solution for clinical practice.This article reviews the principle,basic process,application status,current clinical challenges and development direction of difficult arteriovenous fistulas cannulation guided by POCUS technology,in order to provide reference for the standardized clinical application and future research direction of this technology.
基金supported by the National Natural Science Foundation of China(52103299)。
文摘With the global push for energy conservation and the rapid development of low-power,flexible and wearable optical displays,the demand for electrochromic technology has surged.Gel polymer electrolytes(GPEs),a crucial component of electrochromic devices(ECDs),show great promise in applications.This is attributed to their efficient ion-transport capabilities,excellent mechanical properties and strong adhesion.All of these characteristics are conducive to enhancing the safety of the devices,streamlining the packaging process,significantly improving the electrochromic performance of ECDs and boosting their commercial application potential.This review provides a comprehensive overview of GPEs for ECDs,focusing on their basic designs,functional modifications and practical applications.Firstly,this review outlines the fundamental design of GPEs for ECDs,encompassing key performance index,classification,gelation mechanism and preparation methods.Building on this foundation,it provides an in-depth discussion of functionalized GPEs developed to enhance device performance or expand functionality,including electrochromic,temperature-responsive,photo-responsive and stretchable self-healing GPE.Furthermore,the integration of GPEs into various ECD applications,including smart windows,displays,energy storage devices and wearable electronic,are summarized to highlight the advantages that the design of GPEs brings to the practical application of ECDs.Finally,based on the summary of GPEs employed for ECDs,the challenges and development expectations in this direction were indicated.
基金supported by the financial aid from the National Natural Science Foundation of China (No. 22271273)International Partnership Program of Chinese Academy of Sciences (No. 121522KYSB20190022)。
文摘Self-trapping excitons(STEs) emission in metal halides has been a matter of interest, correlating with the strength of electron-phonon coupling in the lattice, which are usually caused by ions with ns~2 electronic structure. In this work, Sb^(3+)/Te^(4+)ions doped Zn-based halide single crystals(SCs) with two STEs emissions have been synthesized and the possibility of its anti-counterfeiting application was explored.Further, the relationship between the strength of electron-phonon coupling and photoluminescence quantum yields(PLQYs) for STEs in a series of metal halides has been studied. And the semi-empirical range of the Huang-Rhys factors(S) for metal halides with excellent photoluminescence(PL) property has been summarized. This work provides ideas for further research into the relationship between luminescence performance and electron-phonon coupling of metal halides, and also provides a reference for designing the metal halides with high PLQYs.
基金supported by the Science and Technology Special Fund of Hainan Province(Grant Nos.ZDYF2021XDNY159 and ZDYF2021XDNY156)the Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Science(Grant No.1630032022007)China Agricultural Research System of MOF and MARA(Grant No.CARS-32-01)。
文摘Litchi(Litchi chinensis Sonn.),an important fruit tree in tropical and subtropical regions,possesses substantial economic value.The branchand leaf-related traits of litchi have a significant impact on litchi yield and quality.However,due to limitations such as the density of the genetic linkage map,there have been few studies on mapping QTLs of branch-and leaf-related traits.In this study,a high-density genetic map was constructed by next-generation sequencing(NGS)using an F_(1) population of 264 progenies,derived from the cross between the cultivars‘Sanyuehong'and‘Ziniangxi'.A total of 2574 high-quality BINs(binomial intervals)were obtained,and a genetic linkage map was constructed with a total length of 1753.3 cM and an average marker distance of 0.68 cM.With the genetic map and the phenotyping of single leaf length(SLL),single leaf width(SLW),leaf shape index(LSI),weight of specific leaf(WSL),petiole length(PL)and compound leaf length(CLL)measured in three seasons,11,9,9,10,9 and 12 QTLs were detected for SLL,SLW,WSL,LSI,PL and CLL traits,respectively.Among these QTLs,five QTLs were consistently detected in two seasons and 12 pleiotropic QTLs were identified for at least two traits.These findings will provide new insights for the gene cloning for branch-and leaf-related traits as well as marker-assisted selection(MAS).
文摘Fructus Ligustri Lucidi(FLL)refers to the dried mature fruit of Ligustrum lucidum Ait.,a species from the Oleaceae family,widely distributed across East Asia and India.This study aimed to optimize the extraction process for Fructus Ligustri Lucidi essential oil(FLLO)to develop an efficient and practical extraction method.Additionally,the chemical composition of FLLO was analyzed,and its antioxidant,antimicrobial,and cytotoxic activities were evaluated.FLLO was extracted using supercritical CO_(2) extraction,and response surface methodology was applied to optimize the extraction parameters:pressure of 16 MPa,temperature of 40℃,and extraction time of 40 min.The main components of the essential oil were identified through GC-MS analysis.Antioxidant activity was assessed using DPPH and ABTS assays,demonstrating that FLLO exhibited strong antioxidant properties,with a DPPH radical scavenging rate exceeding 80%.In antimicrobial tests,FLLO exhibited significant inhibitory effects on both Gram-positive and Gram-negative bacteria at concentrations greater than 25 mg/mL.Additionally,cytotoxicity assays revealed that FLLO enhanced the proliferation of LO2 cells.In conclusion,FLLO,extracted using supercritical CO_(2),demonstrates excellent antioxidant and antimicrobial properties,as well as favorable cell safety,supporting its potential for further development and application of Ligustrum lucidum.
基金supported by the steady supports scientific research of Key Laboratory of Defense Science and Technology,China(No.WDZC20220105)the National Natural Science Foundation of China(Nos.51775020,62073009,U20B2002)the Science Challenge Project,China(No.TZ2018007)。
文摘Thermal Protection System(TPS)with thick tiles,low thermal conductivity,and a short re-entry stage stands as a critical element within reusable aircraft,whose reliability is related to the function and changes with their physical properties,external conditions,and degradation.Meanwhile,due to the limitation of testing resources,epistemic uncertainties stemming from the small samples are present in TPS reliability modeling.However,current TPS reliability modeling methods face challenges in characterizing the relationships among reliability and physical properties,external conditions,degradation,and epistemic uncertainties.Therefore,under the framework of belief reliability theory,a TPS reliability model is constructed,which takes into account the physical principle,external conditions,performance degradation,and epistemic uncertainties.A reliability simulation algorithm is proposed to calculate TPS reliability.Through a case study and comparison analysis,the proposed method is validated as more effective than the existing method.Additionally,reliability sensitivity analysis is conducted to identify the sensitive factors of reliability under the condition of small samples,through which suggestions are provided for TPS functional design and improvement.
基金supported by the Postdoctoral Fellowship Program of CPSF,China(No.GZC20242033)the National Science and Technology Research Program of China(No.JPPT2023PXY01).
文摘The intergranular corrosion behavior of 2050 Al-Li alloy subjected to non-isothermal aging(NIA)treatment with varying pre-deformation amounts was investigated.Results indicate that the resistance to intergranular corrosion improves with increasing pre-deformation amouts.However,when the pre-deformation amount reaches 20%,the corrosion resistance deteriorates.Microstructural analyses via transmission electron microscopy(TEM)and electron backscatter diffraction(EBSD)reveal that as pre-deformation amount increases,the fraction of high-angle grain boundaries(HAGBs)decreases,while the proportion of low-angle grain boundaries(LAGBs)increases.This change provides additional nucleation sites for precipitates,leading to a reduction in T1 phase size and an increase in T1 phase density.The finer T1 phases contribute to a lower localized potential difference within the grains,slowering corrosion propagation.Furthermore,during corrosion,preferential dissolution of Li results in Cu enrichment along grain boundaries,which further reduces the intergranular corrosion resistance.
基金supported by the Guangdong Major Project of Basic and Applied Basic Research(Grant No.2020B0301030008)the National Natural Science Foundation of China(Grant Nos.11935007 and 12035007)+1 种基金supported by the Open Foundation of the Key Laboratory of Quark and Lepton Physics(MOE)(Grant No.QLPL2023P01)the Talent Scientific Star-up Foundation of the China Three Gorges University(CTGU)(Grant No.2024RCKJ013)。
文摘We present the first theoretical study on heavy-flavor jet angularities(λ_(α)^(κ))in Pb+Pb collisions at√SNN=5.02 Te V.The initial production of heavy-flavor jets was carried out using the POWHEG+PYTHIA8 prescription.In contrast,the SHELL transport model describes jet evolution in the quark-gluon plasma(QGP).In p+p collisions,we observed narrower angularity distributions for D ^(0)-tagged jets than for inclusive jets,consistent with the ALICE preliminary results.We then demonstrate that jet quenching in the QGP slightly widens the angularity distribution of D ^(0)-tagged jets in Pb+Pb collisions relative to that in p+p collisions for a jet transverse momentum of 10<pch/T,jet<20 Ge V/c.In contrast,the angularity distributions of the inclusive and D ^(0)-tagged jets become narrower in Pb+Pb collisions relative to p+p atpch/T,jet>20 Ge V/c because of the strong influence of selection bias.Additionally,by comparing the average angularities<λ_(α)^(κ)>of the inclusive,D ^(0)-tagged,and B ^(0)-tagged jets with varyingαandκ,we show that the larger the quark mass,the lower the jet’s<λ_(α)^(κ)>values are.As a result of the slenderer initial distribution,we predict that compared with inclusive jets,heavy-flavor jets,especially B~0-tagged ones,will suffer stronger modifications of<λ_(α)^(κ)>in Pb+Pb relative to p+p at 10<pch/T,jet<20 Ge V/c.For a larger jet radius,a more significant broadening of the jet angularities was predicted because of the enhanced contribution of the wide-angle particles.
