“智能油田”概念自2003年提出以来,国内外油气企业开展了大量探索,但尚未建成统一定义与真正意义上的“智能油田”。文章在系统回溯油田智能化发展脉络的基础上,结合智能体“基础大语言模型基座+行业大语言模型+任务规划+外部工具调用...“智能油田”概念自2003年提出以来,国内外油气企业开展了大量探索,但尚未建成统一定义与真正意义上的“智能油田”。文章在系统回溯油田智能化发展脉络的基础上,结合智能体“基础大语言模型基座+行业大语言模型+任务规划+外部工具调用+记忆”的核心构成与运行逻辑,提出了以“全过程机器自主管控”为核心的智能油田新定义,构建涵盖感知、通信、数据、模型、智能体集群、控制执行与业务共治的七层体系架构,并提出智能油田自主等级(L1~L5)评价体系,为行业建立可量化的技术标尺。明确了智能油田的建设本质是“钻完井—油藏—井筒—地面—集输—经营”全链条由智能体集群自主管控、人类承担“最后仲裁者”角色的“机器主控、人类监护”模式。通过对Shell的Smart Field、bp的Field of the Future、Saudi Aramco的I-Field等国际公司及以中国石油长庆油田、中国石化胜利油田、中国海油秦皇岛32-6海上油田等国内示范工程的系统对比研究发现,全球范围内虽已普遍实现高水平数字化感知与局部自主控制,但尚未形成自主决策与管控体系,距离智能油田仍存在实质差距。在此基础上,文章提出构建行业基础大模型与智能体集群,实现机理—数据—知识三元融合。通过“虚拟—影子—实控”三阶段实现安全可控的自主权迁移;打造跨域协同的系统级智能,实现产量、能耗、碳排放、安全的多目标自优化。从技术体系、工程路径、组织制度、低碳转型四个维度提出发展方向,构建“2026~2030年准智能油田试点、2031~2035年区域级自主管控、2035年后真正智能油田建成”的三阶段发展路线及目标。本研究成果以期为国内外智能油田建设提供系统性的理论框架、工程路径与政策参考。展开更多
[Objectives]To investigate the anti-hepatic fibrosis mechanism of lavandulyl flavonoid Kurarinol A(KA)from Sophora flavescens through the TGF/Smad signaling pathway.[Methods]A hepatic fibrosis model was established by...[Objectives]To investigate the anti-hepatic fibrosis mechanism of lavandulyl flavonoid Kurarinol A(KA)from Sophora flavescens through the TGF/Smad signaling pathway.[Methods]A hepatic fibrosis model was established by TGF-β1-induced activation of human hepatic stellate cells LX-2.Western blot and RT-qPCR techniques were employed to study the anti-fibrotic mechanism of KA through the TGF/Smad signaling pathway.[Results]KA exerted anti-hepatic fibrosis effects by significantly reducing the gene expression levels of TGF-β1,Smad2,Smad3,and Smad4,as well as markedly decreasing the protein expression levels of TGF-β1,p-Smad2/3/Smad2/3,and Smad4.[Conclusions]KA demonstrates significant anti-hepatic fibrosis activity and alleviates liver fibrosis through the TGF/Smad signaling pathway.展开更多
During the development of diet-induced obesity,the change of energy matebolism is closely related to the function of the circadian clock in mammals.Luteolin(LU),one of the most common natural flavonoids riched in many...During the development of diet-induced obesity,the change of energy matebolism is closely related to the function of the circadian clock in mammals.Luteolin(LU),one of the most common natural flavonoids riched in many edible plants,can ameliorate obesity by activating adipose tissue browning,but its effect on circadian clock in this process remains poorly understood.Here we found that dietary LU improved circadian misalignment of energy expenditure in high-fat diet(HFD)-fed wild-type(WT)mice.Moreover,dietary LU efficiently elevated uncoupling protein 1 levels in adipose tissue during the dark period,which was similar to the LU-increased hepatic PER2 expressions.Hepatic peroxisome proliferators-activated receptorsα(PPARα)/recombinant retinoid X receptorα(RXRα)/fibroblast growth factor 21(FGF21)pathway was rhythmically elevated by dietary LU in HFD-fed WT mice,whereas the promotion was inhibited in Per2^(-/-)mice.Meanwhile,Per2 deletion abolished the effects of dietary LU on adipose tissue browning in HFD-fed mice.Further,LU treatment directly activated PPARα/RXRα/FGF21 signaling in primary cultured hepatocytes from WT mice rather than Per2^(-/-)mice.Taken together,the deletion of the core clock component Per2 impedes LUinduced adipose tissue browning through weakening PPARα/RXRα/FGF21 pathway in mice,providing a new insight into the interplay of energy metabolism and circadian clock for the anti-obesity activity of LU.展开更多
Background Embryo implantation in early gestation is crucial for reproductive success,while dietary fiber plays a key role in regulating sow fertility.However,the underlying mechanisms remain unclear.This study explor...Background Embryo implantation in early gestation is crucial for reproductive success,while dietary fiber plays a key role in regulating sow fertility.However,the underlying mechanisms remain unclear.This study explores the influence of dietary inulin on embryonic implantation using a sow model.Sows were fed a diet supplemented with 11 g/kg of inulin during early gestation and were slaughtered on gestation day 19(G19).Uterine fluid exosomes(UFEs)and endometrial tissues were collected for high-throughput sequencing and for analysis of the expression of angiogenesis-related genes and proteins,respectively.Furthermore,UFEs obtained from slaughtered sows were injected into G19 sows to investigate the effects on reproduction and angiogenesis.Results The results showed that inulin significantly increased the number of blood vessels in the endometrium and expression of the angiogenesis-related proteins MMP2 and ANGPT1 in G19 sows(P<0.05).Bioinformatics analysis revealed that inulin significantly downregulated miRNAs associated with angiogenesis inhibition in UFEs,while upregulating miRNAs related to trophoblast physiological activities and regulation of the uterine fluid microenvironment(P<0.05).Furthermore,intravenous injection of G19 sows with UFEs from sows fed a diet containing inulin had significantly promoted vascular formation in the endometrium and embryos,and increased the number of live embryos on gestation day 28(G28)(P<0.05).Additionally,the mRNA expression levels of MMP2,ANGPT1,and VEGF in the placentas of sows were significantly elevated on G28 and at farrowing in the UFEs injection group(P<0.05).Conclusion Dietary supplementation with inulin during early gestation in sows promoted embryo implantation by regulating angiogenesis at the maternal–fetal interface through the modulation of miRNA expression in UFEs.These findings provide a theoretical reference for the application of dietary fiber in sow nutrition.展开更多
Keratinocytes,the major cell types of the epidermis,proliferate and migrate during wound healing to restore the epithelial barrier.Royal jelly is a traditional remedy used in wound repair.Our previous study found that...Keratinocytes,the major cell types of the epidermis,proliferate and migrate during wound healing to restore the epithelial barrier.Royal jelly is a traditional remedy used in wound repair.Our previous study found that the mixture of major royal jelly protein(MRJP)2,3 and 7 exhibited in vitro wound healing-promoting effects;however,the exact functional constituents and the associated underlying mechanisms of action are still largely unknown.In this study,a partial fragment of MRJP3 was recombinantly expressed as a fusion protein MRJP3-C113-Fc which promoted wound healing in vitro and in vivo.By employing protein inhibitors and immunoblots technology,it was initially found that the wound-repairing mechanisms of MRJP3-C113-Fc were correlated to the activation of EGFR/AKT/m TOR signaling pathway in keratinocytes(Ha Ca T cells).LC-MS/MS-based proteomic analysis demonstrated that the proteins present in the MRJP3-C113-Fc-treated Ha Ca T cells were different from the untreated ones,in which rhomboid 5 homolog 2(RHBDF2)might be the potential regulator for the EGFR/AKT/m TOR signaling pathway.Silencing of RHBDF2 diminished the proproliferative and-migratory effectiveness of MRJP3-C113-Fc on Ha Ca T cells,as well as the phosphorylation of EGFR/AKT/m TOR,suggesting that the wound healing-promoting efficacy was attributable to the RHBDF2-mediated activation of EGFR/AKT/m TOR signaling pathway.Hence,this study is the first to discover the single fragment of MRJPs possessing pro-healing properties,and also the first to disclose the regulatory role of RHBDF2 in EGFR/AKT/m TOR signaling pathway-modulated wound healing.It will facilitate the development of MRJP3-C113-based therapeutic agent for skin wounds,and provide a novel target for treating cutaneous trauma.展开更多
Background Heat stress(HS)is posing as a tremendous threat to the swine industry,due to the thermos-sensitive gonads of boars.Testes are immune-privileged organs in which spermatogenesis needs to remain undisturbed,wh...Background Heat stress(HS)is posing as a tremendous threat to the swine industry,due to the thermos-sensitive gonads of boars.Testes are immune-privileged organs in which spermatogenesis needs to remain undisturbed,whereas immune cells are thermo-sensitive,especially macrophages,which are the most abundant testicular immune cells.Our study aimed to unveil the underlying immune responses and assess their consequences on the semen quality of boars under HS.The results will aid in addressing environmental temperature-related seasonal infertility and in selecting the best boar for use in artificial insemination.Methods The 3-week experiment assigned 268-week-old Rongchang male pigs into thermal neutral pair-feed(TN-PF)and HS groups.During the last 2 weeks,which served as the HS period,the HS group was subjected to 14-day 35±1℃,while the TN-PF group was kept at 26±1℃.Pig gonad tissues were sampled at the end of HS period for assessments and measurements.Results Our findings confirmed HS-related reactions such as elevated respiration rate(P<0.05)and elevated heat shock protein 60(HSP60;P<0.05)and heat shock protein 90(HSP90;P<0.05)expression levels.Sperm motility(P=0.06)and progressive sperms(P=0.08)were decreased under HS as was a significant reduction in average straight-line velocity(VSL;P<0.05).Additionally,total abnormality levels increased(P<0.05).Fibrosis,caspase-3 expression,and accumulations of tumor necrosis factor-α(TNF-α;P<0.05)and interleukin-1β(IL-1β;P<0.05),along with an elevated macrophage composition(P<0.05)characterized the orchitis under HS.Single cell RNA sequencing(scRNA-seq)revealed fluctuations in engulfing and inflammatory signals in testicular macrophages(TMs).In particular,the complement cascade was promoted by CD163+macrophages,resulting in membrane attack complex(C5b-9)assembly(P<0.05).Linear regressions further revealed a negative correlation between C5b-9 and sperm motility(P<0.05),as well as near-negative correlations between the C5b-9 and both progressive motility(P=0.08)and VSL(P=0.06).Conclusions Our findings highlighted the relationship between HS,the onset of orchitis,and the activation of the complement system,all of which decreased the boar semen quality.展开更多
To solve the resource-constrained project scheduling problem(RCPSP),a hybrid ant colony optimization(HACO)approach is presented.To improve the quality of the schedules,the HACO is incorporated with an extended double ...To solve the resource-constrained project scheduling problem(RCPSP),a hybrid ant colony optimization(HACO)approach is presented.To improve the quality of the schedules,the HACO is incorporated with an extended double justification in which the activity splitting is applied to predict whether the schedule could be improved.The HACO is tested on the set of large benchmark problems from the project scheduling problem library(PSPLIB).The computational result shows that the proposed algo-rithm can improve the quality of the schedules efficiently.展开更多
Nonradical reaction driven by peroxymonosulfate(PMS)based advanced oxidation pro-cesses has drawn widespread attention in water treatment due to their inherent advantages,but the degradation behavior and mechanism of ...Nonradical reaction driven by peroxymonosulfate(PMS)based advanced oxidation pro-cesses has drawn widespread attention in water treatment due to their inherent advantages,but the degradation behavior and mechanism of organic pollutants are still unclear.In this study,the performance,intermediates,mechanism and toxicity of tetracycline(TC)degra-dation were thoroughly examined in the constructed magnetic nitrogen-doped porous car-bon/peroxymonosulfate(Co-N/C-PMS)system.The results showed that 85.4%of TC could be removed within 15 min when Co-N/C and PMS was simultaneously added and the degra-dation rate was enhanced by 3.4 and 14.7 folds compared with Co-N/C or PMS alone,re-spectively.Moreover,the performance of Co-N/C was superior to that of most previously reported catalysts.Many lines of evidence indicated that Co-N/C-PMS system was a singlet oxygen-dominated nonradical reaction,which was less interfered by pH and water compo-nents,and displayed high adaptability to actual water bodies.Subsequently,the degrada-tion process was elaborated on the basis of three-dimensional excitation-emission matrix spectra and liquid chromatography-mass spectrometry.At last,the toxicity of treated TC was greatly reduced by using microalgae Coelastrella sp.as ecological indicator.This study provides a promising approach based on singlet oxygen-dominated nonradical reaction for eliminating TC in water treatment.展开更多
OBJECTIVE:To investigate the efficacy of Qingchi San(青赤散,QCS),a preparation of Traditional Chinese Medicine,on ulcerative colitis(UC)in mice by inhibiting the nuclearfactor-kappa B(NF-κB)signaling pathway and nucl...OBJECTIVE:To investigate the efficacy of Qingchi San(青赤散,QCS),a preparation of Traditional Chinese Medicine,on ulcerative colitis(UC)in mice by inhibiting the nuclearfactor-kappa B(NF-κB)signaling pathway and nucleotide-binding oligomerization domain,leucine-rich repeat and pyrin domain-containing 3(NLRP3)inflammasome formation.METHODS:The UC model was established with male C57BL/6J as the animal model.Bodyweight,Disease Activity Index(DAI),colon length and weight were detected.Furthermore,colonic histology was performed by hematoxylin-eosin(HE)staining.interleukin-1β(IL-1β),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),myeloperoxidase(MPO)and superoxide dismutase(SOD)were performed by enzyme-linked immunosorbent assay.Cyclooxygenase 2(COX2)and inducible nitric oxide synthase(iNOS)mRNA expression were conducted by real-time quantitative polymerase chain reaction(RT-qPCR).NF-κB,inhibitor of NF-κBα(iκBα),Phosphorylated inhibitor of NF-κBα(p-iκBα),caspase-1,NLRP3 and Apoptosis-associated speck-like protein containing a caspase recruitment domain(ASC)protein expression were conducted by Western blotting.RESULTS:Compared with UC model group,Bodyweight was significantly increased in QCS treatment.At the same time,DAI was significantly decreased in QCS treatment.Colon length and weight and colonic histology were significantly improved in QCS treatment.Furthermore,the expression of IL-1β,IL-6,TNF-α,MPO,SOD,COX2,and iN OS were significantly decreased in QCS treatment.Finally,the expression of NF-κB signaling pathway-related proteins NF-κB,iκBα,p-iκBα,and the expression of NLRP3 inflammasome related proteins caspase-1,NLRP3 and ASC were significantly decreased in QCS treatment.CONCLUSION:Traditional Chinese drug QCS could treat UC by inhibiting the NF-κB signaling pathway and NLRP3 inflammasome formation in mice.展开更多
In this paper, the authors study the boundedness of a class of multi-sublinear operators on the product of Morrey, Herz-Morrey and generalized Morrey spaces, respectively. As their special cases, the corresponding res...In this paper, the authors study the boundedness of a class of multi-sublinear operators on the product of Morrey, Herz-Morrey and generalized Morrey spaces, respectively. As their special cases, the corresponding results of multilinear Galderón-gygmund operator can be obtained.展开更多
Serotonin is ubiquitous across all forms of life and functions in responses to biotic and abiotic stresses.In rice,the conversion of tryptamine to serotonin is catalyzed by Sekiguchi lesion(SL).Previous studies have i...Serotonin is ubiquitous across all forms of life and functions in responses to biotic and abiotic stresses.In rice,the conversion of tryptamine to serotonin is catalyzed by Sekiguchi lesion(SL).Previous studies have identified an sl mutation(a null mutation of SL)in several rice varieties and confirmed its increase of resistance and cell death.However,a systematic understanding of the reprogrammed cellular processes causing cell death and resistance is lacking.We performed a multi-omics analysis to clarify the fundamental mechanisms at the protein,gene transcript,and metabolite levels.We found that cell death and Magnaporthe oryzae(M.oryzae)infection of the sl-MH-1 mutant activated plant hormone signal transduction involving salicylic acid(SA),jasmonic acid(JA),and abscisic acid(ABA)in multiple regulatory layers.We characterized the dynamic changes of several key hormone levels during disease progression and under the cell death conditions and showed that SA and JA positively regulated rice cell death and disease resistance.SL-overexpressing lines confirmed that the sl-MH-1 mutant positively regulated rice resistance to M.oryzae.Our studies shed light on cell death and facilitate further mechanistic dissection of programmed cell death in rice.展开更多
By employing metal oxides as oxygen carriers,chemical looping demonstrates its effectiveness in transferring oxygen between reduction and oxidation environments to partially oxidize fuels into syngas and convert CO_(2...By employing metal oxides as oxygen carriers,chemical looping demonstrates its effectiveness in transferring oxygen between reduction and oxidation environments to partially oxidize fuels into syngas and convert CO_(2) into CO.Generally,NiFe_(2_)O_(4) oxygen carriers have demonstrated remarkable efficiency in chemical looping CO_(2) conversion.Nevertheless,the intricate process of atomic migration and evolution within the internal structure of bimetallic oxygen carriers during continuous high‐temperature redox cycling remains unclear.Consequently,the lack of a fundamental understanding of the complex ionic migration and oxygen transfer associated with energy conversion processes hampers the design of high‐performance oxygen carriers.Thus,in this study,we employed in situ characterization techniques and theoretical calculations to investigate the ion migration behavior and structural evolution in the bulk of NiFe_(2_)O_(4) oxygen carriers during H_(2) reduction and CO_(2)/lab air oxidation cycles.We discovered that during the H_(2) reduction step,lattice oxygen rapidly migrates to vacancy layers to replenish consumed active oxygen species,while Ni leaches from the material and migrates to the surface.During the CO_(2) splitting step,Ni migrates toward the core of the bimetallic oxygen carrier,forming Fe–Ni alloys.During the air oxidation step,Fe–Ni migrates outward,creating a hollow structure owing to the Kirkendall effect triggered by the swift transfer of lattice oxygen.The metal atom migration paths depend on the oxygen transfer rates.These discoveries highlight the significance of regulating the release–recovery rate of lattice oxygen to uphold the structures and reactivity of oxygen carriers.This work offers a comprehensive understanding of the oxidation/reduction‐driven atomic interdiffusion behavior of bimetallic oxygen carriers.展开更多
Background:Dietary fiber(DF)is often eschewed in swine diet due to its anti-nutritional effects,but DF is attracting growing attention for its reproductive benefits.The objective of this study was to investigate the e...Background:Dietary fiber(DF)is often eschewed in swine diet due to its anti-nutritional effects,but DF is attracting growing attention for its reproductive benefits.The objective of this study was to investigate the effects of DF intake level on oocyte maturation and uterine development,to determine the optimal DF intake for gilts,and gain microbial and metabolomic insight into the underlying mechanisms involved.Methods:Seventy-six Landrace×Yorkshire(LY)crossbred replacement gilts of similar age(92.6±0.6 d;mean±standard deviation[SD])and body weight(BW,33.8±3.9 kg;mean±SD)were randomly allocated to 4 dietary treatment groups(n=19);a basal diet without extra DF intake(DF 1.0),and 3 dietary groups ingesting an extra50%(DF 1.5),75%(DF 1.75),and 100%(DF 2.0)dietary fiber mixture consisting of inulin and cellulose(1:4).Oocyte maturation and uterine development were assessed on 19 d of the 2nd oestrous cycle.Microbial diversity of faecal samples was analysed by high-throughput pyrosequencing(16S r RNA)and blood samples were subjected to untargeted metabolomics.Results:The rates of oocytes showing first polar bodies after in vitro maturation for 44 h and uterine development increased linearly with increasing DF intake;DF 1.75 gilts had a 19.8%faster oocyte maturation rate and a 48.9 cm longer uterus than DF 1.0 gilts(P<0.05).Among the top 10 microbiota components at the phylum level,8increased linearly with increasing DF level,and the relative abundance of 30 of 53 microbiota components at the genus level(>0.1%)increased linearly or quadratically with increasing DF intake.Untargeted metabolic analysis revealed significant changes in serum metabolites that were closely associated with microbiota,including serotonin,a gut-derived signal that stimulates oocyte maturation.Conclusions:The findings provide evidence of the benefits of increased DF intake by supplementing inulin and cellulose on oocyte maturation and uterine development in gilts,and new microbial and metabolomic insight into the mechanisms mediating the effects of DF on reproductive performance of replacement gilts.展开更多
Background:Success in the National Qualified Examination for Registered Professional Nurses(NQEX-RPN)is a critical issue for each nursing student and every nursing school.However,to date,little research has been condu...Background:Success in the National Qualified Examination for Registered Professional Nurses(NQEX-RPN)is a critical issue for each nursing student and every nursing school.However,to date,little research has been conducted regarding the degree of risk for licensure failure associated with specific academic courses and clinical internships in China's Mainland.Objective:This study aimed to explore the relationship of academic courses and clinical internships to performance on the NQEX-RPN in China's Mainland.Methods:This was a retrospective descriptive study.Two hundred twenty-eight new graduates were recruited froma four-year baccalaureate nursing programin China's Mainland.The scores of academic courses and clinical internships were recorded from the Teaching and Learning administration of the university.The NQEX-RPN performance data was collected from the Provincial Health Department.Personal information was collected from the participants.Multiple stepwise regression was used to identify predictors of the NQEX-RPN.Results:Twenty-three courses and four clinical internships were positively associated with NQEX-RPN performance.Surgical nursing,Medical nursing,Computer basics and Community health care explained the 36.2%of the total variance of Theoretical Knowledge(F=31.313,p=0.000);clinical internships of obstetrics and gynecology nursing,pediatrics and surgical nursing explained the 28.1%of the total variance of Application Skills(F=28.852,p=0.000).Conclusion:Surgical-medicine nursing,Community health care,Computer basics and clinical internships are beneficial in assisting students and educators to identify the risk of students'performance in the NQEX-RPN.展开更多
文摘“智能油田”概念自2003年提出以来,国内外油气企业开展了大量探索,但尚未建成统一定义与真正意义上的“智能油田”。文章在系统回溯油田智能化发展脉络的基础上,结合智能体“基础大语言模型基座+行业大语言模型+任务规划+外部工具调用+记忆”的核心构成与运行逻辑,提出了以“全过程机器自主管控”为核心的智能油田新定义,构建涵盖感知、通信、数据、模型、智能体集群、控制执行与业务共治的七层体系架构,并提出智能油田自主等级(L1~L5)评价体系,为行业建立可量化的技术标尺。明确了智能油田的建设本质是“钻完井—油藏—井筒—地面—集输—经营”全链条由智能体集群自主管控、人类承担“最后仲裁者”角色的“机器主控、人类监护”模式。通过对Shell的Smart Field、bp的Field of the Future、Saudi Aramco的I-Field等国际公司及以中国石油长庆油田、中国石化胜利油田、中国海油秦皇岛32-6海上油田等国内示范工程的系统对比研究发现,全球范围内虽已普遍实现高水平数字化感知与局部自主控制,但尚未形成自主决策与管控体系,距离智能油田仍存在实质差距。在此基础上,文章提出构建行业基础大模型与智能体集群,实现机理—数据—知识三元融合。通过“虚拟—影子—实控”三阶段实现安全可控的自主权迁移;打造跨域协同的系统级智能,实现产量、能耗、碳排放、安全的多目标自优化。从技术体系、工程路径、组织制度、低碳转型四个维度提出发展方向,构建“2026~2030年准智能油田试点、2031~2035年区域级自主管控、2035年后真正智能油田建成”的三阶段发展路线及目标。本研究成果以期为国内外智能油田建设提供系统性的理论框架、工程路径与政策参考。
基金Supported by Guizhou Provincial Science and Technology Project(2024-023ZK2024-047,2024-015)+3 种基金the Innovation and Entrepreneurship Training Program for Undergraduates from China(202310660082,S2024106601432X)University Engineering Research Center for the Prevention and Treatment of Chronic Diseases by Authentic Medicinal Materials in Guizhou Province(2023-035)Administration of Traditional Chinese Medicine of Guizhou Province(QZYY-2024-134)Science Foundation of the Health Commission of Guizhou Province(gzwkj2025-538).
文摘[Objectives]To investigate the anti-hepatic fibrosis mechanism of lavandulyl flavonoid Kurarinol A(KA)from Sophora flavescens through the TGF/Smad signaling pathway.[Methods]A hepatic fibrosis model was established by TGF-β1-induced activation of human hepatic stellate cells LX-2.Western blot and RT-qPCR techniques were employed to study the anti-fibrotic mechanism of KA through the TGF/Smad signaling pathway.[Results]KA exerted anti-hepatic fibrosis effects by significantly reducing the gene expression levels of TGF-β1,Smad2,Smad3,and Smad4,as well as markedly decreasing the protein expression levels of TGF-β1,p-Smad2/3/Smad2/3,and Smad4.[Conclusions]KA demonstrates significant anti-hepatic fibrosis activity and alleviates liver fibrosis through the TGF/Smad signaling pathway.
文摘During the development of diet-induced obesity,the change of energy matebolism is closely related to the function of the circadian clock in mammals.Luteolin(LU),one of the most common natural flavonoids riched in many edible plants,can ameliorate obesity by activating adipose tissue browning,but its effect on circadian clock in this process remains poorly understood.Here we found that dietary LU improved circadian misalignment of energy expenditure in high-fat diet(HFD)-fed wild-type(WT)mice.Moreover,dietary LU efficiently elevated uncoupling protein 1 levels in adipose tissue during the dark period,which was similar to the LU-increased hepatic PER2 expressions.Hepatic peroxisome proliferators-activated receptorsα(PPARα)/recombinant retinoid X receptorα(RXRα)/fibroblast growth factor 21(FGF21)pathway was rhythmically elevated by dietary LU in HFD-fed WT mice,whereas the promotion was inhibited in Per2^(-/-)mice.Meanwhile,Per2 deletion abolished the effects of dietary LU on adipose tissue browning in HFD-fed mice.Further,LU treatment directly activated PPARα/RXRα/FGF21 signaling in primary cultured hepatocytes from WT mice rather than Per2^(-/-)mice.Taken together,the deletion of the core clock component Per2 impedes LUinduced adipose tissue browning through weakening PPARα/RXRα/FGF21 pathway in mice,providing a new insight into the interplay of energy metabolism and circadian clock for the anti-obesity activity of LU.
基金supported by the National Natural Science Foundation of China (32102554)Sichuan Province “145” Breeding Tackle Project (2021YFYZ0008)China Agriculture Research System (CARS-35).
文摘Background Embryo implantation in early gestation is crucial for reproductive success,while dietary fiber plays a key role in regulating sow fertility.However,the underlying mechanisms remain unclear.This study explores the influence of dietary inulin on embryonic implantation using a sow model.Sows were fed a diet supplemented with 11 g/kg of inulin during early gestation and were slaughtered on gestation day 19(G19).Uterine fluid exosomes(UFEs)and endometrial tissues were collected for high-throughput sequencing and for analysis of the expression of angiogenesis-related genes and proteins,respectively.Furthermore,UFEs obtained from slaughtered sows were injected into G19 sows to investigate the effects on reproduction and angiogenesis.Results The results showed that inulin significantly increased the number of blood vessels in the endometrium and expression of the angiogenesis-related proteins MMP2 and ANGPT1 in G19 sows(P<0.05).Bioinformatics analysis revealed that inulin significantly downregulated miRNAs associated with angiogenesis inhibition in UFEs,while upregulating miRNAs related to trophoblast physiological activities and regulation of the uterine fluid microenvironment(P<0.05).Furthermore,intravenous injection of G19 sows with UFEs from sows fed a diet containing inulin had significantly promoted vascular formation in the endometrium and embryos,and increased the number of live embryos on gestation day 28(G28)(P<0.05).Additionally,the mRNA expression levels of MMP2,ANGPT1,and VEGF in the placentas of sows were significantly elevated on G28 and at farrowing in the UFEs injection group(P<0.05).Conclusion Dietary supplementation with inulin during early gestation in sows promoted embryo implantation by regulating angiogenesis at the maternal–fetal interface through the modulation of miRNA expression in UFEs.These findings provide a theoretical reference for the application of dietary fiber in sow nutrition.
基金supported by the grants from the Natural Science Foundation of Fujian Province(2023J01490,2019J01408)the Central Guidance on Local Science and Technology Development Fund of Fujian Province(2024L3006)+3 种基金the Science and Technology Innovation Special Fund of Fujian Agriculture and Forestry University(KFb22071XA)the Outstanding Young Scientist Program of Fujian Agriculture and Forestry University(xjq201916)the National Natural Science Foundation of China(32272938)the China Agriculture Research System of MOF,and MARA(CARS-44)。
文摘Keratinocytes,the major cell types of the epidermis,proliferate and migrate during wound healing to restore the epithelial barrier.Royal jelly is a traditional remedy used in wound repair.Our previous study found that the mixture of major royal jelly protein(MRJP)2,3 and 7 exhibited in vitro wound healing-promoting effects;however,the exact functional constituents and the associated underlying mechanisms of action are still largely unknown.In this study,a partial fragment of MRJP3 was recombinantly expressed as a fusion protein MRJP3-C113-Fc which promoted wound healing in vitro and in vivo.By employing protein inhibitors and immunoblots technology,it was initially found that the wound-repairing mechanisms of MRJP3-C113-Fc were correlated to the activation of EGFR/AKT/m TOR signaling pathway in keratinocytes(Ha Ca T cells).LC-MS/MS-based proteomic analysis demonstrated that the proteins present in the MRJP3-C113-Fc-treated Ha Ca T cells were different from the untreated ones,in which rhomboid 5 homolog 2(RHBDF2)might be the potential regulator for the EGFR/AKT/m TOR signaling pathway.Silencing of RHBDF2 diminished the proproliferative and-migratory effectiveness of MRJP3-C113-Fc on Ha Ca T cells,as well as the phosphorylation of EGFR/AKT/m TOR,suggesting that the wound healing-promoting efficacy was attributable to the RHBDF2-mediated activation of EGFR/AKT/m TOR signaling pathway.Hence,this study is the first to discover the single fragment of MRJPs possessing pro-healing properties,and also the first to disclose the regulatory role of RHBDF2 in EGFR/AKT/m TOR signaling pathway-modulated wound healing.It will facilitate the development of MRJP3-C113-based therapeutic agent for skin wounds,and provide a novel target for treating cutaneous trauma.
基金supported by the Projects of The National Natural Science Foundation of China(U21A20255)Strategic Priority Research Program of the National Center of Technology Innovation for Pigs(NCTIPXD/B04)+3 种基金The National Natural Science Foundation of China(32573270)The National Natural Science Foundation of China(3227291)National Modern Agricultural Industry Technology System Sichuan Pig innovation team(SCSZTD-2024-08)the National Key R&D Program of China(2023YFD1300804)。
文摘Background Heat stress(HS)is posing as a tremendous threat to the swine industry,due to the thermos-sensitive gonads of boars.Testes are immune-privileged organs in which spermatogenesis needs to remain undisturbed,whereas immune cells are thermo-sensitive,especially macrophages,which are the most abundant testicular immune cells.Our study aimed to unveil the underlying immune responses and assess their consequences on the semen quality of boars under HS.The results will aid in addressing environmental temperature-related seasonal infertility and in selecting the best boar for use in artificial insemination.Methods The 3-week experiment assigned 268-week-old Rongchang male pigs into thermal neutral pair-feed(TN-PF)and HS groups.During the last 2 weeks,which served as the HS period,the HS group was subjected to 14-day 35±1℃,while the TN-PF group was kept at 26±1℃.Pig gonad tissues were sampled at the end of HS period for assessments and measurements.Results Our findings confirmed HS-related reactions such as elevated respiration rate(P<0.05)and elevated heat shock protein 60(HSP60;P<0.05)and heat shock protein 90(HSP90;P<0.05)expression levels.Sperm motility(P=0.06)and progressive sperms(P=0.08)were decreased under HS as was a significant reduction in average straight-line velocity(VSL;P<0.05).Additionally,total abnormality levels increased(P<0.05).Fibrosis,caspase-3 expression,and accumulations of tumor necrosis factor-α(TNF-α;P<0.05)and interleukin-1β(IL-1β;P<0.05),along with an elevated macrophage composition(P<0.05)characterized the orchitis under HS.Single cell RNA sequencing(scRNA-seq)revealed fluctuations in engulfing and inflammatory signals in testicular macrophages(TMs).In particular,the complement cascade was promoted by CD163+macrophages,resulting in membrane attack complex(C5b-9)assembly(P<0.05).Linear regressions further revealed a negative correlation between C5b-9 and sperm motility(P<0.05),as well as near-negative correlations between the C5b-9 and both progressive motility(P=0.08)and VSL(P=0.06).Conclusions Our findings highlighted the relationship between HS,the onset of orchitis,and the activation of the complement system,all of which decreased the boar semen quality.
基金supported by Liaoning BaiQianWan Talents Program(20071866-25)
文摘To solve the resource-constrained project scheduling problem(RCPSP),a hybrid ant colony optimization(HACO)approach is presented.To improve the quality of the schedules,the HACO is incorporated with an extended double justification in which the activity splitting is applied to predict whether the schedule could be improved.The HACO is tested on the set of large benchmark problems from the project scheduling problem library(PSPLIB).The computational result shows that the proposed algo-rithm can improve the quality of the schedules efficiently.
基金This work was supported by the National Natural Science Foundation of China(Nos.51978178 and 51521006)the Department of Science and Technology of Guangdong Province of China(Nos.2019A1515012044 and 2021A1515011797)+3 种基金the International S&T Cooperation Program of China(No.2015DFG92750)the Maoming Municipal Bureau of Science and Technology of Guangdong of China(No.2018S0013)the Startup Fund of Guangdong University of Petrochemical Technology(No.2020rc041)the Shanghai Tongji Gao Tingyao Environmental Science&Technology Development Foundation.
文摘Nonradical reaction driven by peroxymonosulfate(PMS)based advanced oxidation pro-cesses has drawn widespread attention in water treatment due to their inherent advantages,but the degradation behavior and mechanism of organic pollutants are still unclear.In this study,the performance,intermediates,mechanism and toxicity of tetracycline(TC)degra-dation were thoroughly examined in the constructed magnetic nitrogen-doped porous car-bon/peroxymonosulfate(Co-N/C-PMS)system.The results showed that 85.4%of TC could be removed within 15 min when Co-N/C and PMS was simultaneously added and the degra-dation rate was enhanced by 3.4 and 14.7 folds compared with Co-N/C or PMS alone,re-spectively.Moreover,the performance of Co-N/C was superior to that of most previously reported catalysts.Many lines of evidence indicated that Co-N/C-PMS system was a singlet oxygen-dominated nonradical reaction,which was less interfered by pH and water compo-nents,and displayed high adaptability to actual water bodies.Subsequently,the degrada-tion process was elaborated on the basis of three-dimensional excitation-emission matrix spectra and liquid chromatography-mass spectrometry.At last,the toxicity of treated TC was greatly reduced by using microalgae Coelastrella sp.as ecological indicator.This study provides a promising approach based on singlet oxygen-dominated nonradical reaction for eliminating TC in water treatment.
基金Supported by the National Key Research and Development Program(Grant 2018YFC1705403):Clinical Effect Evaluation of Chinese Medicine on Moderately Active Ulcerative ColitisNatural Science Foundation of Tianjin(Grant18JCYBJC26000):Study on the Mechanism of Qingchi San Treating Ulcerative Colitis By Regulating Gut Microflora+1 种基金Scientific Research Program of Tianjin Education Commission(Grant 2017KJ155):based onγδT-NLRP3 Inflammasome-Caspase PathwayQingchi San Regulates Gut Microflora to Treat UC。
文摘OBJECTIVE:To investigate the efficacy of Qingchi San(青赤散,QCS),a preparation of Traditional Chinese Medicine,on ulcerative colitis(UC)in mice by inhibiting the nuclearfactor-kappa B(NF-κB)signaling pathway and nucleotide-binding oligomerization domain,leucine-rich repeat and pyrin domain-containing 3(NLRP3)inflammasome formation.METHODS:The UC model was established with male C57BL/6J as the animal model.Bodyweight,Disease Activity Index(DAI),colon length and weight were detected.Furthermore,colonic histology was performed by hematoxylin-eosin(HE)staining.interleukin-1β(IL-1β),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),myeloperoxidase(MPO)and superoxide dismutase(SOD)were performed by enzyme-linked immunosorbent assay.Cyclooxygenase 2(COX2)and inducible nitric oxide synthase(iNOS)mRNA expression were conducted by real-time quantitative polymerase chain reaction(RT-qPCR).NF-κB,inhibitor of NF-κBα(iκBα),Phosphorylated inhibitor of NF-κBα(p-iκBα),caspase-1,NLRP3 and Apoptosis-associated speck-like protein containing a caspase recruitment domain(ASC)protein expression were conducted by Western blotting.RESULTS:Compared with UC model group,Bodyweight was significantly increased in QCS treatment.At the same time,DAI was significantly decreased in QCS treatment.Colon length and weight and colonic histology were significantly improved in QCS treatment.Furthermore,the expression of IL-1β,IL-6,TNF-α,MPO,SOD,COX2,and iN OS were significantly decreased in QCS treatment.Finally,the expression of NF-κB signaling pathway-related proteins NF-κB,iκBα,p-iκBα,and the expression of NLRP3 inflammasome related proteins caspase-1,NLRP3 and ASC were significantly decreased in QCS treatment.CONCLUSION:Traditional Chinese drug QCS could treat UC by inhibiting the NF-κB signaling pathway and NLRP3 inflammasome formation in mice.
基金Supported by the the National Natural Science Foundation of China (10571014) the Doctoral Programme Foundation of Institution of Higher Education of China (20040027001).
文摘In this paper, the authors study the boundedness of a class of multi-sublinear operators on the product of Morrey, Herz-Morrey and generalized Morrey spaces, respectively. As their special cases, the corresponding results of multilinear Galderón-gygmund operator can be obtained.
基金supported by the Collaborative Innovation Engineering“5511”(XTCXGC2021002)the National Natural Science Foundation of China(U1805232)+1 种基金the Youth Program of National Natural Science Foundation of China(31301654)the Youth Program of Fujian Academy of Agricultural Sciences(YC2019004)。
文摘Serotonin is ubiquitous across all forms of life and functions in responses to biotic and abiotic stresses.In rice,the conversion of tryptamine to serotonin is catalyzed by Sekiguchi lesion(SL).Previous studies have identified an sl mutation(a null mutation of SL)in several rice varieties and confirmed its increase of resistance and cell death.However,a systematic understanding of the reprogrammed cellular processes causing cell death and resistance is lacking.We performed a multi-omics analysis to clarify the fundamental mechanisms at the protein,gene transcript,and metabolite levels.We found that cell death and Magnaporthe oryzae(M.oryzae)infection of the sl-MH-1 mutant activated plant hormone signal transduction involving salicylic acid(SA),jasmonic acid(JA),and abscisic acid(ABA)in multiple regulatory layers.We characterized the dynamic changes of several key hormone levels during disease progression and under the cell death conditions and showed that SA and JA positively regulated rice cell death and disease resistance.SL-overexpressing lines confirmed that the sl-MH-1 mutant positively regulated rice resistance to M.oryzae.Our studies shed light on cell death and facilitate further mechanistic dissection of programmed cell death in rice.
基金National Natural Science Foundation of China,Grant/Award Numbers:52076209,52006224,52106285,22179027Foundation and Applied Foundation Research of Guangdong Province,Grant/Award Number:2022B1515020045+1 种基金Natural Science Foundation of Guangxi Province,Grant/Award Number:2021GXNSFAA075036Young Talent Support Project of Guangzhou Association for Science and Technology,Grant/Award Number:QT‐2023‐042。
文摘By employing metal oxides as oxygen carriers,chemical looping demonstrates its effectiveness in transferring oxygen between reduction and oxidation environments to partially oxidize fuels into syngas and convert CO_(2) into CO.Generally,NiFe_(2_)O_(4) oxygen carriers have demonstrated remarkable efficiency in chemical looping CO_(2) conversion.Nevertheless,the intricate process of atomic migration and evolution within the internal structure of bimetallic oxygen carriers during continuous high‐temperature redox cycling remains unclear.Consequently,the lack of a fundamental understanding of the complex ionic migration and oxygen transfer associated with energy conversion processes hampers the design of high‐performance oxygen carriers.Thus,in this study,we employed in situ characterization techniques and theoretical calculations to investigate the ion migration behavior and structural evolution in the bulk of NiFe_(2_)O_(4) oxygen carriers during H_(2) reduction and CO_(2)/lab air oxidation cycles.We discovered that during the H_(2) reduction step,lattice oxygen rapidly migrates to vacancy layers to replenish consumed active oxygen species,while Ni leaches from the material and migrates to the surface.During the CO_(2) splitting step,Ni migrates toward the core of the bimetallic oxygen carrier,forming Fe–Ni alloys.During the air oxidation step,Fe–Ni migrates outward,creating a hollow structure owing to the Kirkendall effect triggered by the swift transfer of lattice oxygen.The metal atom migration paths depend on the oxygen transfer rates.These discoveries highlight the significance of regulating the release–recovery rate of lattice oxygen to uphold the structures and reactivity of oxygen carriers.This work offers a comprehensive understanding of the oxidation/reduction‐driven atomic interdiffusion behavior of bimetallic oxygen carriers.
基金supported by the Sichuan Science and Technology Program(2021YJ0287)National Natural Science Foundation of ChinaPR China(31772616)。
文摘Background:Dietary fiber(DF)is often eschewed in swine diet due to its anti-nutritional effects,but DF is attracting growing attention for its reproductive benefits.The objective of this study was to investigate the effects of DF intake level on oocyte maturation and uterine development,to determine the optimal DF intake for gilts,and gain microbial and metabolomic insight into the underlying mechanisms involved.Methods:Seventy-six Landrace×Yorkshire(LY)crossbred replacement gilts of similar age(92.6±0.6 d;mean±standard deviation[SD])and body weight(BW,33.8±3.9 kg;mean±SD)were randomly allocated to 4 dietary treatment groups(n=19);a basal diet without extra DF intake(DF 1.0),and 3 dietary groups ingesting an extra50%(DF 1.5),75%(DF 1.75),and 100%(DF 2.0)dietary fiber mixture consisting of inulin and cellulose(1:4).Oocyte maturation and uterine development were assessed on 19 d of the 2nd oestrous cycle.Microbial diversity of faecal samples was analysed by high-throughput pyrosequencing(16S r RNA)and blood samples were subjected to untargeted metabolomics.Results:The rates of oocytes showing first polar bodies after in vitro maturation for 44 h and uterine development increased linearly with increasing DF intake;DF 1.75 gilts had a 19.8%faster oocyte maturation rate and a 48.9 cm longer uterus than DF 1.0 gilts(P<0.05).Among the top 10 microbiota components at the phylum level,8increased linearly with increasing DF level,and the relative abundance of 30 of 53 microbiota components at the genus level(>0.1%)increased linearly or quadratically with increasing DF intake.Untargeted metabolic analysis revealed significant changes in serum metabolites that were closely associated with microbiota,including serotonin,a gut-derived signal that stimulates oocyte maturation.Conclusions:The findings provide evidence of the benefits of increased DF intake by supplementing inulin and cellulose on oocyte maturation and uterine development in gilts,and new microbial and metabolomic insight into the mechanisms mediating the effects of DF on reproductive performance of replacement gilts.
文摘Background:Success in the National Qualified Examination for Registered Professional Nurses(NQEX-RPN)is a critical issue for each nursing student and every nursing school.However,to date,little research has been conducted regarding the degree of risk for licensure failure associated with specific academic courses and clinical internships in China's Mainland.Objective:This study aimed to explore the relationship of academic courses and clinical internships to performance on the NQEX-RPN in China's Mainland.Methods:This was a retrospective descriptive study.Two hundred twenty-eight new graduates were recruited froma four-year baccalaureate nursing programin China's Mainland.The scores of academic courses and clinical internships were recorded from the Teaching and Learning administration of the university.The NQEX-RPN performance data was collected from the Provincial Health Department.Personal information was collected from the participants.Multiple stepwise regression was used to identify predictors of the NQEX-RPN.Results:Twenty-three courses and four clinical internships were positively associated with NQEX-RPN performance.Surgical nursing,Medical nursing,Computer basics and Community health care explained the 36.2%of the total variance of Theoretical Knowledge(F=31.313,p=0.000);clinical internships of obstetrics and gynecology nursing,pediatrics and surgical nursing explained the 28.1%of the total variance of Application Skills(F=28.852,p=0.000).Conclusion:Surgical-medicine nursing,Community health care,Computer basics and clinical internships are beneficial in assisting students and educators to identify the risk of students'performance in the NQEX-RPN.
