This study primarily aimed to investigate the prevalence of human papillomavirus(HPV)and other common pathogens of sexually transmitted infections(STIs)in spermatozoa of infertile men and their effects on semen parame...This study primarily aimed to investigate the prevalence of human papillomavirus(HPV)and other common pathogens of sexually transmitted infections(STIs)in spermatozoa of infertile men and their effects on semen parameters.These pathogens included Ureaplasma urealyticum,Ureaplasma parvum,Chlamydia trachomatis,Mycoplasma genitalium,herpes simplex virus 2,Neisseria gonorrhoeae,Enterococcus faecalis,Streptococcus agalactiae,Pseudomonas aeruginosa,and Staphylococcus aureus.A total of 1951 men of infertile couples were recruited between 23 March 2023,and 17 May 2023,at the Department of Reproductive Medicine of The First People’s Hospital of Yunnan Province(Kunming,China).Multiplex polymerase chain reaction and capillary electrophoresis were used for HPV genotyping.Polymerase chain reaction and electrophoresis were also used to detect the presence of other STIs.The overall prevalence of HPV infection was 12.4%.The top five prevalent HPV subtypes were types 56,52,43,16,and 53 among those tested positive for HPV.Other common infections with high prevalence rates were Ureaplasma urealyticum(28.3%),Ureaplasma parvum(20.4%),and Enterococcus faecalis(9.5%).The prevalence rates of HPV coinfection with Ureaplasma urealyticum,Ureaplasma parvum,Chlamydia trachomatis,Mycoplasma genitalium,herpes simplex virus 2,Neisseria gonorrhoeae,Enterococcus faecalis,Streptococcus agalactiae,and Staphylococcus aureus were 24.8%,25.4%,10.6%,6.4%,2.4%,7.9%,5.9%,0.9%,and 1.3%,respectively.The semen volume and total sperm count were greatly decreased by HPV infection alone.Coinfection with HPV and Ureaplasma urealyticum significantly reduced sperm motility and viability.Our study shows that coinfection with STIs is highly prevalent in the semen of infertile men and that coinfection with pathogens can seriously affect semen parameters,emphasizing the necessity of semen screening for STIs.展开更多
BACKGROUND Liver fibrosis is a global health issue that lacks effective treatments.Tibetan medicine,with a long history,has accumulated rich experience in the treatment of chronic liver diseases.The saffron(Saf)and Ca...BACKGROUND Liver fibrosis is a global health issue that lacks effective treatments.Tibetan medicine,with a long history,has accumulated rich experience in the treatment of chronic liver diseases.The saffron(Saf)and Calculus bovis(Cal b)combination is among the most commonly used medicines in clinical practice in Tibetan medicine for hepatic disease.Its characteristic therapies and drug compatibility provide unique ideas for the treatment of liver fibrosis and have research value and application potential.AIM To investigate the efficacy of the Saf-Cal b therapy in treating liver fibrosis and explored its underlying mechanism.METHODS We initially established a carbon tetrachloride-induced rat liver fibrosis model to assess Saf-Cal b’s anti-fibrotic effects.Subsequently,we conducted network pharmacology analysis to identify the potential therapeutic targets and pathways of Saf-Cal b in liver fibrosis intervention.Finally,we performed in vivo validation of key regulatory targets.RESULTS Saf-Cal b combination therapy exerted superior effects in ameliorating liver fibrosis in model rats compared with Saf or Cal b monotherapy.Through network pharmacology prediction,key targets of the combination were identified.Mechanistic validation revealed that Saf-Cal b inhibited the p38 mitogen-activated protein kinases pathway,which in turn suppressed the transforming growth factor-β/small mother against decapentaplegic pathway.This sequential inhibition led to reduced activation of hepatic stellate cells,a central event in liver fibrosis progression.CONCLUSION These findings demonstrate that Saf-Cal b combination therapy is more effective than either monotherapy in alleviating liver fibrosis,with its therapeutic effect mediated through the p38 mitogen-activated protein kinases/transforming growth factor-β/small mother against decapentaplegic signaling axis,providing a potential therapeutic strategy for liver fibrosis.展开更多
The position-dependent feature in current vat photopolymerization-based additive manufacturing leads to challenges in controlling the dimensional accuracy of printed components.To overcome this intrinsic limitation,we...The position-dependent feature in current vat photopolymerization-based additive manufacturing leads to challenges in controlling the dimensional accuracy of printed components.To overcome this intrinsic limitation,we propose a time-dependent dynamic laser writing(DLW)approach for the precise volumetric printing of complex-shaped lenses.In the DLW-based volumetric printing,the formed surface is generated by accumulating the material growth functions(MGFs)on the scanning path,where the MGF is created by the laser direct irradiation with controlled energy doses.Benefiting from the stability of MGFs and the process homogenization,the DLW is less sensitive to process errors when compared to current vat photopolymerization-based additive manufacturing techniques.Furthermore,the continuous scanning leads to the naturally ultra-smooth feature of the printed surfaces.As a demonstration,a millimeter-scale spherical lens was printed in 5.67 min,achieving a three-dimensional(3D)form error of 0.135μm(root mean square,RMS)and a surface roughness of 0.31 nm(RMS).The printing demonstrated comparable efficiency while achieving form errors an order of magnitude smaller than those of state-of-the-art continuous layer-wise and volumetric printing methods.In addition,polymer lens arrays,freeform polymer lenses,and fused silica lenses were successfully printed,demonstrating promise for advancing the state-of-the-art in 3D printing of precision lenses.展开更多
Xiamen, located on the southeastern coastal line of China, is undergoing rapid urbanization and industrialization, so its air quality has a trend of degradation. However, studies on level, temporal and spatial changes...Xiamen, located on the southeastern coastal line of China, is undergoing rapid urbanization and industrialization, so its air quality has a trend of degradation. However, studies on level, temporal and spatial changes of fine particles （PM2.5） and their carbonaceous fractions are scarce. In this article, abundance, sources, seasonal and spatial variations, distribution of organic carbon （OC） and elemental carbon （EC） in PM2.5, were studied at suburban, urban and industrial sites in Xiamen during four season-representative months in 2009-2010. PM2.5 samples were collected with middle volume sampler and were analyzed for OC and EC with thermal optical transmittance （TOT） method. Results showed that the annual average PM2.5 concentrations were 63.88-74.80 Ixg/m3 at three sites. While OC and EC concentrations were in the range of 15.81-19.73 [xg/m3 and 2.74-3.49 ~tg/m3, respectively, and clearly presented the summer minima and winter maxima in this study. The carbonaceous aerosol accounted for 42.8%-47.3% of the mass of PMzs. The annual average of secondary organic carbon （SOC） concentrations in Xiamen were 9.23-11.36 ~g/m3, accounting for approximately 56% of OC. Strong correlations between OC and EC was found in spring （R2 = 0.50） and autumn （R2 = 0.73）, suggesting that there were similar emission and transport processes for carbonaceous aerosols in these two seasons, while weak correlations were found in summer （R2 = 0.33） and winter （R2 = 0.41）. The OCI＇EC ratios in PM2.5 varied from 2.1 to 8.7 with an annual average of 5.7, indicating that vehicle exhaust, coal smoke and biomass burning were main source apportionments of carbonaceous fractions in Xiamen.展开更多
An intensive sampling program had been undertaken in autumn （October, 2008） and winter （December, 2008 and January, 2009） at urban （Xiamen University and Xianyue residential area）, suburban （Institute of Urban ...An intensive sampling program had been undertaken in autumn （October, 2008） and winter （December, 2008 and January, 2009） at urban （Xiamen University and Xianyue residential area）, suburban （Institute of Urban Environment）, industrial area （Lulian Hotel） and background （Tingxi Reservoir） in Xiamen, Fujian Province, to characterize the atmospheric concentration and gas-particle phase partitioning of PAHs. The average concentration of total PAHs in winter was almost 1.7 times higher than those in autumn. The log scale plot of Kp versus sub-cooled liquid vapor pressure （PL^0） for all the data of autumn and winter season samples gave significantly different slopes. The slope for the winter samples （-0.72） was steeper than that for the autumn samples （-0.58）. The partitioning results indicated that slope values varied depending on characteristics of specific site, source region and meteorological conditions which play important roles in the partitioning of PAHs. In addition, local emission sources had a stronger effect on partitioning results than long-transported polluted plume. The sources of PAHs in five sampling sites in Xiamen also have been discussed initially. Diagnostic ratios showed that the primary source of PAHs in urban, suburban and industrial area was from vehicle exhausts. While emission from petrochemical factory and power plant was another main contributor to industrial area.展开更多
In this work,experimental and theoretical studies were carried out on arc-induced bubble dynamic behaviors in insulation oil.Direct experimental evidence indicated that the arc-induced bubble experiences pulsating gro...In this work,experimental and theoretical studies were carried out on arc-induced bubble dynamic behaviors in insulation oil.Direct experimental evidence indicated that the arc-induced bubble experiences pulsating growth rather than a continuous expansion.Furthermore,a theoretical model and numerical calculation method were proposed,which revealed the dynamic mechanism of bubble growth.Good agreement between the theoretical results and experimental observations verified the general correctness and feasibility of the proposed method.展开更多
Kidney-tonifying recipe can reduce the accumulation of advanced glycation end products, prevent neuronal degeneration and improve cognitive functions in ovariectomized rats. Radix Achyranthis Bidentatae alcohol extrac...Kidney-tonifying recipe can reduce the accumulation of advanced glycation end products, prevent neuronal degeneration and improve cognitive functions in ovariectomized rats. Radix Achyranthis Bidentatae alcohol extracts may dose-dependently inhibit non-enzymatic saccharification in vitro. This study aimed to examine the effect of Radix Achyranthis Bidentatae on advanced glycation end products and on learning and memory capabilities in ovariectomized rats. Ovariectomized rats were treated with Radix Achyranthis Bidentatae alcohol extracts （containing 1.5 g/kg crude drug） or 0.1% aminoguanidine for 12 weeks and behavioral testing was performed with the Y-electrical maze. This test revealed that Radix Achyranthis Bidentatae and aminoguanidine could improve the learning and memory capabilities of ovariectomized rats. Results of competitive enzyme-linked immunosorbent assay showed that treatment with Radix Achyranthis Bidentatae or aminoguanidine reduced the accumulation of advanced glycation end products in the frontal cortex of ovariectomized rats, while increasing content in the blood and urine. Biochemical tests showed that treatment with Radix Achyranthis Bidentatae or aminoguanidine decreased superoxide dismutase activity in the serum and frontal cortex, and increased serum levels of glutathione peroxidase in ovariectomized rats. In addition there was no apparent effect on malondialdehyde levels. These experimental findings indicate that Radix Achyranthis Bidentatae inhibits production of advanced glycation end products and its accumulation in brain tissue, and improves learning and memory capabilities in ovariectomized rats. These effects may be associated with an anti-oxidative action of the extract.展开更多
BACKGROUND Liver fibrosis is a common health problem worldwide and there is still a lack of effective medicines.The Chinese herbal medicine,Gan Shen Fu Fang(GSFF)is composed of salvianolic acid B and diammonium glycyr...BACKGROUND Liver fibrosis is a common health problem worldwide and there is still a lack of effective medicines.The Chinese herbal medicine,Gan Shen Fu Fang(GSFF)is composed of salvianolic acid B and diammonium glycyrrhizinate.In this study,we observed the effects of GSFF on liver fibrosis in vivo and in vitro in an attempt to provide some hope for the treatment.AIM To observe the effects of GSFF on liver fibrosis in vivo and in vitro and investigate the mechanism from the perspective of the inflammatory response and extracellular signal-regulated kinase(ERK)phosphorylation.METHODS Common bile duct-ligated rats were used for in vivo experiments.Hepatic stellate cells-T6(HSC-T6)cells were used for in vitro experiments.Hematoxylin and eosin staining and Masson staining,biochemical assays,hydroxyproline(Hyp)assays,enzyme-linked immunoasorbent assay and western blotting were performed to evaluate the degree of liver fibrosis,liver function,the inflammatory response and ERK phosphorylation.The CCK8 assay,immunofluorescence and western blotting were applied to test the effect of GSFF on HSC-T6 cell activation and determine whether GSFF had an effect on ERK phosphorylation in HSC-T6 cells.RESULTS GSFF improved liver function and inhibited liver fibrosis in common bile ductligated rats after 3 wk of treatment,as demonstrated by histological changes,hydroxyproline assays and collagen I concentrations.GSFF alleviated inflammatory cell infiltration and reduced the synthesis of pro-inflammatory cytokines[tumor necrosis factor-α(TNF-α)and interlukin-1β]and NF-κB.In addition,GSFF decreased ERK phosphorylation.In vitro,GSFF inhibited the viability of HSC-T6 cells with and without transforming growth factorβ1(TGF-β1)stimulation and decreased the synthesis of collagen I.GSFF had the greatest effect at a concentration of 0.5μmol/L.GSFF inhibited the expression ofα-smooth muscle actin(α-SMA),a marker of HSC activation,in HSC-T6 cells.Consistent with the in vivo results,GSFF also inhibited the phosphorylation of ERK and downregulated the expression of NF-κB.CONCLUSION GSFF inhibited liver fibrosis progression in vivo and HSC-T6 cell activation in vitro.These effects may be related to an alleviated inflammatory response and downregulated ERK phosphorylation.展开更多
In this study,we present a new method for the indirect integration of beam charges in external beam proton-induced X-ray emission and proton-induced c-ray emission(PIXE–PIGE) analysis.We recorded proton spectra backs...In this study,we present a new method for the indirect integration of beam charges in external beam proton-induced X-ray emission and proton-induced c-ray emission(PIXE–PIGE) analysis.We recorded proton spectra backscattered by a Kapton film extraction window in different sample situations and under different beam currents.We also simulated backscattering spectra using the simulation of backscattering spectra program(SIMNRA).We determined that in a specific geometrical arrangement,different sample situations did not significantly affect factor C_Q(the ratio between integral backscattering proton counts and integral beam charges).We also studied the reproducibility and beam current dependence of factor C_Q.The statistic factor of C_Q was28.95 ± 0.6 kilo counts/l C,with a relative standard deviation of 2.0 %.Significantly,in external beam PIXE–PIGE analysis,we were able to calculate beam charge integration from the integral backscattering proton counts in an energy region.展开更多
[ Objective] This study aimed to investigate the effects of heat shock factor AtHsfAla on Caspase-3 activity in Arabidopsis thaliana under high tempera-ture stress, thus revealing the relationship between heat shoc...[ Objective] This study aimed to investigate the effects of heat shock factor AtHsfAla on Caspase-3 activity in Arabidopsis thaliana under high tempera-ture stress, thus revealing the relationship between heat shock factor AtHsfAl a and programmed cell death in A. thaliana. [ Method ] Different genotypes of A. thaliana （AtHsfAla-silenced transgenic and wild-type） seedlings were treated at 42 ℃. According to the fragmentation level of fluorogenic substrate Ac-DEVD- pNA, Caspase-3 activity was determined by spectrophotometry. [ Result] After high temperature treatment, Caspase-3 activity in A. thaliana was enhanced signifi-cantly. Caspase-3 activity in AtHsfAla-si／enced transgenic A. thaliana was higher than that in wild-type A. thaliana, which indicated that AtHsfAla could inhibit Caspase-3 activity in A. thaliana under high temperature stress. [ Conclusion] Under high temperature stress, heat shock factor AtHsfAla might exert inhibitory effects on programmed cell death by reducing Caspase-3 activity. This study provided the basis for clarifying the mechanism of stress resistance in plants.展开更多
Microwave ablation(MWA)status monitoring in real time plays a key role in assessment of therapeutic fectiveness.As a novel realtime assessment method,near infrared spectroscopy(NIRs)was used to evaluate the ablation e...Microwave ablation(MWA)status monitoring in real time plays a key role in assessment of therapeutic fectiveness.As a novel realtime assessment method,near infrared spectroscopy(NIRs)was used to evaluate the ablation eficacy.MWA experiments were carried out on in vitroporcine livers.An optical measurement system for biological tissue is developed by our lab tomonitor reduced scattering coefficient(μ')at 690 nm of the coagulation zones.It is noted that p's of liver tissue,which increases as the liver tissue being ablated,is clearly related with the co-agulation status.μ's of normal tissue and coagulated tissue is 3-5 and 17-19 cm-1,respectively.Continuous changes ofμ's demonstrate that optical parameter can be used as an efficacy evaluation factor because it essentially indicates the degree of thermal damage.Compared withtemperat ure,optical parameter is more sensitive and accurate,which is promising for real-timetherapeutic eficacy assessment in MWA.展开更多
Cell migration is a finely tuned biological process that often involves epithelial-mesenchymal transition(EMT).EMT is typically characterized by the upregulation of mesenchymal markers such as Snail1.This process has ...Cell migration is a finely tuned biological process that often involves epithelial-mesenchymal transition(EMT).EMT is typically characterized by the upregulation of mesenchymal markers such as Snail1.This process has been shown to be of critical importance to normal developmental processes,including neural crest migration and invasion.Interestingly,similar mechanisms are utilized in disease processes,such as tumor metastasis and migration.Notably,EMT and EMT-like processes confer tumor cells with the ability to migrate,invade,and adopt stem cell-like properties that largely account for immunosuppression and tumor recurrence.Therefore,identifying sensitive EMT markers may contribute to cancer prognosis and diagnosis in many facets.Previously,we showed that Elp3 plays an essential role during neural crest migration by stabilizing Snail1.In the current study,we further elucidate the molecular mechanism underlying colorectal cancer migration.We found that mElp3 exerted an identical function to xElp3 in modulating neural crest migration,and both HAT and SAM domains are imperative during this migratory process.Interestingly,overexpression of mElp3 in SW480 cells promoted cell migration and invasion,and we further showed that Elp3 stabilized Snail1 requiring integrity of both SAM and HAT domains.Our findings warrant further exploration of the specific target of Elp3 in cancer cells.展开更多
The presence of interstitial electrons in electrides endows them with interesting attributes,such as low work function,high carrier concentration,and unique magnetic properties.Thorough knowledge and understanding of ...The presence of interstitial electrons in electrides endows them with interesting attributes,such as low work function,high carrier concentration,and unique magnetic properties.Thorough knowledge and understanding of electrides are thus of both scientific and technological significance.Here,we employ first-principles calculations to investigate Mott-insulating Ae_(5)X_(3)(Ae=Ca,Sr,and Ba;X=As and Sb)electrides with Mn_(5)Si_(3)-type structure,in which half-filled interstitial electrons serve as ions and are spin-polarized.The Mott-insulating property is induced by strong electron correlation between the nearest interstitial electrons,resulting in spin splitting and a separation between occupied and unoccupied states.The half-filled antiferromagnetic configuration and localization of the interstitial electrons are critical for the Mott-insulating properties of these materials.Compared with that in intermetallic electrides,the orbital hybridization between the half-filled interstitial electrons and the surrounding atoms is weak,leading to highly localized magnetic centers and pronounced correlation effects.Therefore,the Mott-insulating electrides Ae_(5)X_(3)have very large indirect bandgaps(0.30 eV).In addition,high pressure is found to strengthen the strong correlation effects and enlarge the bandgap.The present results provide a deeper understanding of the formation mechanism of Mott-insulating electrides and provide guidance for the search for new strongly correlated electrides.展开更多
Objective:Meta-analysis was performed to evaluate the safety of clopidogrel and tigrel in the treatment of non-ST-segment elevation acute coronary syndrome after intervention.Methods:by searching the databases of cnki...Objective:Meta-analysis was performed to evaluate the safety of clopidogrel and tigrel in the treatment of non-ST-segment elevation acute coronary syndrome after intervention.Methods:by searching the databases of cnki,wanfang database,weipu network,bailian and so on,relevant literatures on oral clopidogrel sulfate and tigrel after interventional treatment of non-ST-elevation acute coronary syndrome were collected.Search the required literature according to the inclusion criteria and exclusion criteria.Risk assessment of the included literature was performed on the basis of Cochrane reviews or the Newcastle scale(NOS).Fixed two researchers randomly and the control group,experimental group into literature alone assessed,Use software Review Manager 5.3 analyze aspirin and clopidogrel,aspirin and ticagrelor drug for the safety of the drug after therapy intervention Meta analysis,merge effect value odds ratio(OR)and 95%confidence interval,inspection heterogeneity and publication bias.Results:a total of 16 articles met the criteria,and 3007 patients were included in the meta-analysis.Compared with the clopidogrel sulfate group,the tigrilol group significantly reduced the incidence of major cardiovascular adverse events after interventional therapy for non-ST-segment elevation acute coronary syndrome(MACE)OR=0.37,95%Cl=(0.280.49)(P<0.00001)、Angina symptoms OR=0.46,95%Cl=(0.260.81)(P=0.008)、Non-fatal heart infarction OR=0.38,95%cl=(0.260.81)(P=0.001)、heart failure OR=0.41,95%cl=(0.180.96)(P=0.04)、cardiac death OR=0.31,95%cl=(0.150.64)(P=0.002)、target vessel revascularization OR=0.46,95%cl=(0.210.99)(P=0.05),However,increased respiratory distress OR=4.44,95%cl=(2.089.45)(P<0.0001)、bleeding events OR=2.06,95%cl=(1.303.27)(P=0.002)、arrhythmia OR=1.14,95%cl=(0.851.54)(P=0.39).Conclusion:In the treatment of non-ST-segment elevation acute coronary syndrome after interventional surgery,compared with clopidogrel,Tigliero can reduce the incidence of major cardiovascular adverse events,angina symptoms,non-fatal cardiac infarction,heart failure,cardiogenic death,and target vessel revascularization,However,the incidence of dyspnea,bleeding events and arrhythmias increased.展开更多
[ Objective] This study aimed to screen target genes regulated by heat shock factor AtHsfAla in Arabidopsis thaliana. [ Method] Using AtHsfAla-in- serted mutant athsfala （SALK-068042） and wild-type A. thaliana seedl...[ Objective] This study aimed to screen target genes regulated by heat shock factor AtHsfAla in Arabidopsis thaliana. [ Method] Using AtHsfAla-in- serted mutant athsfala （SALK-068042） and wild-type A. thaliana seedlings as experimental materials, target genes regulated by heat shock factor AtHsfAla were screened by microarray assay. Differentially expressed genes were screened by multiple method. Specific functions of differentially expressed genes were analyzed by gene ontology （GO） analysis. Signal transduction pathways, in which differentia｜ly expressed genes were involved, were analyzed by pathway analysis. Gene-gene interaction network was constructed by Signal-Net. [ Result] A total of 3 672 differentially expressed genes were screened out. Up-regulated differentially expressed genes were involved in 198 functions and 7 signal transduction pathways; down-regulated differentially expressed genes were involved in 94 functions and 10 signal transduction pathways. In the signal transduction network, it was found that cwlNV4 and HXK3 had relatively high ability of mediation; AT1 G14240 and cwlNV4 ex- hibited the most interactions with other genes, which were located in key positions throughout the gene-gene interaction network. [ Conclusion] Heat shock factor AtHsfAla regulates a large number of target genes in A. thaliana.展开更多
基金supported by the Yunnan Provincial Key Laboratory of Clinical Virology(No.202002AG070062)the Key Projects of Yunnan Province Science and Technology Department(No.202302AA310044)the National Natural Science Foundation of China(No.82060664).
文摘This study primarily aimed to investigate the prevalence of human papillomavirus(HPV)and other common pathogens of sexually transmitted infections(STIs)in spermatozoa of infertile men and their effects on semen parameters.These pathogens included Ureaplasma urealyticum,Ureaplasma parvum,Chlamydia trachomatis,Mycoplasma genitalium,herpes simplex virus 2,Neisseria gonorrhoeae,Enterococcus faecalis,Streptococcus agalactiae,Pseudomonas aeruginosa,and Staphylococcus aureus.A total of 1951 men of infertile couples were recruited between 23 March 2023,and 17 May 2023,at the Department of Reproductive Medicine of The First People’s Hospital of Yunnan Province(Kunming,China).Multiplex polymerase chain reaction and capillary electrophoresis were used for HPV genotyping.Polymerase chain reaction and electrophoresis were also used to detect the presence of other STIs.The overall prevalence of HPV infection was 12.4%.The top five prevalent HPV subtypes were types 56,52,43,16,and 53 among those tested positive for HPV.Other common infections with high prevalence rates were Ureaplasma urealyticum(28.3%),Ureaplasma parvum(20.4%),and Enterococcus faecalis(9.5%).The prevalence rates of HPV coinfection with Ureaplasma urealyticum,Ureaplasma parvum,Chlamydia trachomatis,Mycoplasma genitalium,herpes simplex virus 2,Neisseria gonorrhoeae,Enterococcus faecalis,Streptococcus agalactiae,and Staphylococcus aureus were 24.8%,25.4%,10.6%,6.4%,2.4%,7.9%,5.9%,0.9%,and 1.3%,respectively.The semen volume and total sperm count were greatly decreased by HPV infection alone.Coinfection with HPV and Ureaplasma urealyticum significantly reduced sperm motility and viability.Our study shows that coinfection with STIs is highly prevalent in the semen of infertile men and that coinfection with pathogens can seriously affect semen parameters,emphasizing the necessity of semen screening for STIs.
基金Supported by Tibetan Medicine Administration of Tibet Autonomous Region,No.JJKT2020006Key Research and Development Project of Tibet Autonomous Region,No.XZ202201ZY0019GNational Administration of Traditional Chinese Medicine High-level Key Discipline Construction Project,No.zyyzdxk-2023262.
文摘BACKGROUND Liver fibrosis is a global health issue that lacks effective treatments.Tibetan medicine,with a long history,has accumulated rich experience in the treatment of chronic liver diseases.The saffron(Saf)and Calculus bovis(Cal b)combination is among the most commonly used medicines in clinical practice in Tibetan medicine for hepatic disease.Its characteristic therapies and drug compatibility provide unique ideas for the treatment of liver fibrosis and have research value and application potential.AIM To investigate the efficacy of the Saf-Cal b therapy in treating liver fibrosis and explored its underlying mechanism.METHODS We initially established a carbon tetrachloride-induced rat liver fibrosis model to assess Saf-Cal b’s anti-fibrotic effects.Subsequently,we conducted network pharmacology analysis to identify the potential therapeutic targets and pathways of Saf-Cal b in liver fibrosis intervention.Finally,we performed in vivo validation of key regulatory targets.RESULTS Saf-Cal b combination therapy exerted superior effects in ameliorating liver fibrosis in model rats compared with Saf or Cal b monotherapy.Through network pharmacology prediction,key targets of the combination were identified.Mechanistic validation revealed that Saf-Cal b inhibited the p38 mitogen-activated protein kinases pathway,which in turn suppressed the transforming growth factor-β/small mother against decapentaplegic pathway.This sequential inhibition led to reduced activation of hepatic stellate cells,a central event in liver fibrosis progression.CONCLUSION These findings demonstrate that Saf-Cal b combination therapy is more effective than either monotherapy in alleviating liver fibrosis,with its therapeutic effect mediated through the p38 mitogen-activated protein kinases/transforming growth factor-β/small mother against decapentaplegic signaling axis,providing a potential therapeutic strategy for liver fibrosis.
基金supported by the Special funding for Jiangsu Province Innovation Support Program(Grant No.BZ2023058)the National Natural Science Foundation of China(Grant Nos.52275437 and U2013211)。
文摘The position-dependent feature in current vat photopolymerization-based additive manufacturing leads to challenges in controlling the dimensional accuracy of printed components.To overcome this intrinsic limitation,we propose a time-dependent dynamic laser writing(DLW)approach for the precise volumetric printing of complex-shaped lenses.In the DLW-based volumetric printing,the formed surface is generated by accumulating the material growth functions(MGFs)on the scanning path,where the MGF is created by the laser direct irradiation with controlled energy doses.Benefiting from the stability of MGFs and the process homogenization,the DLW is less sensitive to process errors when compared to current vat photopolymerization-based additive manufacturing techniques.Furthermore,the continuous scanning leads to the naturally ultra-smooth feature of the printed surfaces.As a demonstration,a millimeter-scale spherical lens was printed in 5.67 min,achieving a three-dimensional(3D)form error of 0.135μm(root mean square,RMS)and a surface roughness of 0.31 nm(RMS).The printing demonstrated comparable efficiency while achieving form errors an order of magnitude smaller than those of state-of-the-art continuous layer-wise and volumetric printing methods.In addition,polymer lens arrays,freeform polymer lenses,and fused silica lenses were successfully printed,demonstrating promise for advancing the state-of-the-art in 3D printing of precision lenses.
基金supported by the Knowledge Innovation Program of the Chinese Academy of Sciences (No. KZCX2-YW-453,KZCX2-YW-JS404,KZCX2-EW-408)the Commonweal Program of Environment Protection Department of China (No. 201009004)the Program of Bureau of Science and Technology,Xiamen (No. 3502Z20081117,350205Z20095001)
文摘Xiamen, located on the southeastern coastal line of China, is undergoing rapid urbanization and industrialization, so its air quality has a trend of degradation. However, studies on level, temporal and spatial changes of fine particles （PM2.5） and their carbonaceous fractions are scarce. In this article, abundance, sources, seasonal and spatial variations, distribution of organic carbon （OC） and elemental carbon （EC） in PM2.5, were studied at suburban, urban and industrial sites in Xiamen during four season-representative months in 2009-2010. PM2.5 samples were collected with middle volume sampler and were analyzed for OC and EC with thermal optical transmittance （TOT） method. Results showed that the annual average PM2.5 concentrations were 63.88-74.80 Ixg/m3 at three sites. While OC and EC concentrations were in the range of 15.81-19.73 [xg/m3 and 2.74-3.49 ~tg/m3, respectively, and clearly presented the summer minima and winter maxima in this study. The carbonaceous aerosol accounted for 42.8%-47.3% of the mass of PMzs. The annual average of secondary organic carbon （SOC） concentrations in Xiamen were 9.23-11.36 ~g/m3, accounting for approximately 56% of OC. Strong correlations between OC and EC was found in spring （R2 = 0.50） and autumn （R2 = 0.73）, suggesting that there were similar emission and transport processes for carbonaceous aerosols in these two seasons, while weak correlations were found in summer （R2 = 0.33） and winter （R2 = 0.41）. The OCI＇EC ratios in PM2.5 varied from 2.1 to 8.7 with an annual average of 5.7, indicating that vehicle exhaust, coal smoke and biomass burning were main source apportionments of carbonaceous fractions in Xiamen.
基金supported by the Knowledge Innovation Program of the Chinese Academy of Sciences (No.KZCX2-YW-422-4)the Fujian Natural Science Foundation for Young Scientists (No. 2009J05104)the Program of Science and Technology Bureau of Xiamen(No. 3502Z20081117)
文摘An intensive sampling program had been undertaken in autumn （October, 2008） and winter （December, 2008 and January, 2009） at urban （Xiamen University and Xianyue residential area）, suburban （Institute of Urban Environment）, industrial area （Lulian Hotel） and background （Tingxi Reservoir） in Xiamen, Fujian Province, to characterize the atmospheric concentration and gas-particle phase partitioning of PAHs. The average concentration of total PAHs in winter was almost 1.7 times higher than those in autumn. The log scale plot of Kp versus sub-cooled liquid vapor pressure （PL^0） for all the data of autumn and winter season samples gave significantly different slopes. The slope for the winter samples （-0.72） was steeper than that for the autumn samples （-0.58）. The partitioning results indicated that slope values varied depending on characteristics of specific site, source region and meteorological conditions which play important roles in the partitioning of PAHs. In addition, local emission sources had a stronger effect on partitioning results than long-transported polluted plume. The sources of PAHs in five sampling sites in Xiamen also have been discussed initially. Diagnostic ratios showed that the primary source of PAHs in urban, suburban and industrial area was from vehicle exhausts. While emission from petrochemical factory and power plant was another main contributor to industrial area.
基金supported by National Natural Science Foundation of China(No.51807151)。
文摘In this work,experimental and theoretical studies were carried out on arc-induced bubble dynamic behaviors in insulation oil.Direct experimental evidence indicated that the arc-induced bubble experiences pulsating growth rather than a continuous expansion.Furthermore,a theoretical model and numerical calculation method were proposed,which revealed the dynamic mechanism of bubble growth.Good agreement between the theoretical results and experimental observations verified the general correctness and feasibility of the proposed method.
基金financially supported by the National Science and Technology Major Project for New Drug Creation Program by the Ministry of Science and Technology No.2009ZX09502-014
文摘Kidney-tonifying recipe can reduce the accumulation of advanced glycation end products, prevent neuronal degeneration and improve cognitive functions in ovariectomized rats. Radix Achyranthis Bidentatae alcohol extracts may dose-dependently inhibit non-enzymatic saccharification in vitro. This study aimed to examine the effect of Radix Achyranthis Bidentatae on advanced glycation end products and on learning and memory capabilities in ovariectomized rats. Ovariectomized rats were treated with Radix Achyranthis Bidentatae alcohol extracts （containing 1.5 g/kg crude drug） or 0.1% aminoguanidine for 12 weeks and behavioral testing was performed with the Y-electrical maze. This test revealed that Radix Achyranthis Bidentatae and aminoguanidine could improve the learning and memory capabilities of ovariectomized rats. Results of competitive enzyme-linked immunosorbent assay showed that treatment with Radix Achyranthis Bidentatae or aminoguanidine reduced the accumulation of advanced glycation end products in the frontal cortex of ovariectomized rats, while increasing content in the blood and urine. Biochemical tests showed that treatment with Radix Achyranthis Bidentatae or aminoguanidine decreased superoxide dismutase activity in the serum and frontal cortex, and increased serum levels of glutathione peroxidase in ovariectomized rats. In addition there was no apparent effect on malondialdehyde levels. These experimental findings indicate that Radix Achyranthis Bidentatae inhibits production of advanced glycation end products and its accumulation in brain tissue, and improves learning and memory capabilities in ovariectomized rats. These effects may be associated with an anti-oxidative action of the extract.
基金Supported by the Innovation Team of the Beijing University of Chinese Medicine,No.2019-JYB-TD-006the National Natural Science Foundation of China,No.81873099Scientific Research Support Plan for the Construction of Doctoral Program of University of Tibetan Medicine.
文摘BACKGROUND Liver fibrosis is a common health problem worldwide and there is still a lack of effective medicines.The Chinese herbal medicine,Gan Shen Fu Fang(GSFF)is composed of salvianolic acid B and diammonium glycyrrhizinate.In this study,we observed the effects of GSFF on liver fibrosis in vivo and in vitro in an attempt to provide some hope for the treatment.AIM To observe the effects of GSFF on liver fibrosis in vivo and in vitro and investigate the mechanism from the perspective of the inflammatory response and extracellular signal-regulated kinase(ERK)phosphorylation.METHODS Common bile duct-ligated rats were used for in vivo experiments.Hepatic stellate cells-T6(HSC-T6)cells were used for in vitro experiments.Hematoxylin and eosin staining and Masson staining,biochemical assays,hydroxyproline(Hyp)assays,enzyme-linked immunoasorbent assay and western blotting were performed to evaluate the degree of liver fibrosis,liver function,the inflammatory response and ERK phosphorylation.The CCK8 assay,immunofluorescence and western blotting were applied to test the effect of GSFF on HSC-T6 cell activation and determine whether GSFF had an effect on ERK phosphorylation in HSC-T6 cells.RESULTS GSFF improved liver function and inhibited liver fibrosis in common bile ductligated rats after 3 wk of treatment,as demonstrated by histological changes,hydroxyproline assays and collagen I concentrations.GSFF alleviated inflammatory cell infiltration and reduced the synthesis of pro-inflammatory cytokines[tumor necrosis factor-α(TNF-α)and interlukin-1β]and NF-κB.In addition,GSFF decreased ERK phosphorylation.In vitro,GSFF inhibited the viability of HSC-T6 cells with and without transforming growth factorβ1(TGF-β1)stimulation and decreased the synthesis of collagen I.GSFF had the greatest effect at a concentration of 0.5μmol/L.GSFF inhibited the expression ofα-smooth muscle actin(α-SMA),a marker of HSC activation,in HSC-T6 cells.Consistent with the in vivo results,GSFF also inhibited the phosphorylation of ERK and downregulated the expression of NF-κB.CONCLUSION GSFF inhibited liver fibrosis progression in vivo and HSC-T6 cell activation in vitro.These effects may be related to an alleviated inflammatory response and downregulated ERK phosphorylation.
基金supported by the Beijing Natural Science Funds(No.1142006)
文摘In this study,we present a new method for the indirect integration of beam charges in external beam proton-induced X-ray emission and proton-induced c-ray emission(PIXE–PIGE) analysis.We recorded proton spectra backscattered by a Kapton film extraction window in different sample situations and under different beam currents.We also simulated backscattering spectra using the simulation of backscattering spectra program(SIMNRA).We determined that in a specific geometrical arrangement,different sample situations did not significantly affect factor C_Q(the ratio between integral backscattering proton counts and integral beam charges).We also studied the reproducibility and beam current dependence of factor C_Q.The statistic factor of C_Q was28.95 ± 0.6 kilo counts/l C,with a relative standard deviation of 2.0 %.Significantly,in external beam PIXE–PIGE analysis,we were able to calculate beam charge integration from the integral backscattering proton counts in an energy region.
基金Supported by National Natural Science Foundation of China(31260061,31060039)Project of Key Laboratory of Special Biological Resource Development and Utilization of Universities in Yunnan Province(GXZD201601)+1 种基金Key Discipline Construction Project of Kunming UniversityNational College Students' Innovation Project of China
文摘[ Objective] This study aimed to investigate the effects of heat shock factor AtHsfAla on Caspase-3 activity in Arabidopsis thaliana under high tempera-ture stress, thus revealing the relationship between heat shock factor AtHsfAl a and programmed cell death in A. thaliana. [ Method ] Different genotypes of A. thaliana （AtHsfAla-silenced transgenic and wild-type） seedlings were treated at 42 ℃. According to the fragmentation level of fluorogenic substrate Ac-DEVD- pNA, Caspase-3 activity was determined by spectrophotometry. [ Result] After high temperature treatment, Caspase-3 activity in A. thaliana was enhanced signifi-cantly. Caspase-3 activity in AtHsfAla-si／enced transgenic A. thaliana was higher than that in wild-type A. thaliana, which indicated that AtHsfAla could inhibit Caspase-3 activity in A. thaliana under high temperature stress. [ Conclusion] Under high temperature stress, heat shock factor AtHsfAla might exert inhibitory effects on programmed cell death by reducing Caspase-3 activity. This study provided the basis for clarifying the mechanism of stress resistance in plants.
基金part of the project 61378092 supported by NSFC and also funded by the construction,project,"Microwave Ablation Clinical Application Exploration in the Comprehensive Treatment of Cancer",based on the researchplat form of the Afiliated Tumor Hospital of Xiangya Medical College,Central South Universitythe Funding of Jiangsu Innovation Program for Graduate Education(No.CXLX13_147)the Fundamental Research Funds for the Central Universities.
文摘Microwave ablation(MWA)status monitoring in real time plays a key role in assessment of therapeutic fectiveness.As a novel realtime assessment method,near infrared spectroscopy(NIRs)was used to evaluate the ablation eficacy.MWA experiments were carried out on in vitroporcine livers.An optical measurement system for biological tissue is developed by our lab tomonitor reduced scattering coefficient(μ')at 690 nm of the coagulation zones.It is noted that p's of liver tissue,which increases as the liver tissue being ablated,is clearly related with the co-agulation status.μ's of normal tissue and coagulated tissue is 3-5 and 17-19 cm-1,respectively.Continuous changes ofμ's demonstrate that optical parameter can be used as an efficacy evaluation factor because it essentially indicates the degree of thermal damage.Compared withtemperat ure,optical parameter is more sensitive and accurate,which is promising for real-timetherapeutic eficacy assessment in MWA.
基金supported by the National Natural Science Foundation of China(Grant Nos.81760503 and 81760660)the Applied Basic Research Key Project of Yunnan(Grant Nos.2018FE001(-318)and 2018FE001(-123))the Yunnan Health Science and Technology Plan Projects(Grant No.2016NS207).
文摘Cell migration is a finely tuned biological process that often involves epithelial-mesenchymal transition(EMT).EMT is typically characterized by the upregulation of mesenchymal markers such as Snail1.This process has been shown to be of critical importance to normal developmental processes,including neural crest migration and invasion.Interestingly,similar mechanisms are utilized in disease processes,such as tumor metastasis and migration.Notably,EMT and EMT-like processes confer tumor cells with the ability to migrate,invade,and adopt stem cell-like properties that largely account for immunosuppression and tumor recurrence.Therefore,identifying sensitive EMT markers may contribute to cancer prognosis and diagnosis in many facets.Previously,we showed that Elp3 plays an essential role during neural crest migration by stabilizing Snail1.In the current study,we further elucidate the molecular mechanism underlying colorectal cancer migration.We found that mElp3 exerted an identical function to xElp3 in modulating neural crest migration,and both HAT and SAM domains are imperative during this migratory process.Interestingly,overexpression of mElp3 in SW480 cells promoted cell migration and invasion,and we further showed that Elp3 stabilized Snail1 requiring integrity of both SAM and HAT domains.Our findings warrant further exploration of the specific target of Elp3 in cancer cells.
基金This work was supported by the National Natural Science Foundation of China(Grant Nos.12204419 and 12074013)。
文摘The presence of interstitial electrons in electrides endows them with interesting attributes,such as low work function,high carrier concentration,and unique magnetic properties.Thorough knowledge and understanding of electrides are thus of both scientific and technological significance.Here,we employ first-principles calculations to investigate Mott-insulating Ae_(5)X_(3)(Ae=Ca,Sr,and Ba;X=As and Sb)electrides with Mn_(5)Si_(3)-type structure,in which half-filled interstitial electrons serve as ions and are spin-polarized.The Mott-insulating property is induced by strong electron correlation between the nearest interstitial electrons,resulting in spin splitting and a separation between occupied and unoccupied states.The half-filled antiferromagnetic configuration and localization of the interstitial electrons are critical for the Mott-insulating properties of these materials.Compared with that in intermetallic electrides,the orbital hybridization between the half-filled interstitial electrons and the surrounding atoms is weak,leading to highly localized magnetic centers and pronounced correlation effects.Therefore,the Mott-insulating electrides Ae_(5)X_(3)have very large indirect bandgaps(0.30 eV).In addition,high pressure is found to strengthen the strong correlation effects and enlarge the bandgap.The present results provide a deeper understanding of the formation mechanism of Mott-insulating electrides and provide guidance for the search for new strongly correlated electrides.
基金National Natural Science Foundation of China(No.81660722)Natural Science Foundation of Tibet Autonomous Region(No.2015ZR-13-16)+2 种基金Scientific research project of Shaanxi Administration of Traditional Chinese Medicine(No.2019-ZZ-JC046)Key project of Xizang Minzu University(13myZP07)National Innovative Experimental Training Program for College Students(No.201810695031)
文摘Objective:Meta-analysis was performed to evaluate the safety of clopidogrel and tigrel in the treatment of non-ST-segment elevation acute coronary syndrome after intervention.Methods:by searching the databases of cnki,wanfang database,weipu network,bailian and so on,relevant literatures on oral clopidogrel sulfate and tigrel after interventional treatment of non-ST-elevation acute coronary syndrome were collected.Search the required literature according to the inclusion criteria and exclusion criteria.Risk assessment of the included literature was performed on the basis of Cochrane reviews or the Newcastle scale(NOS).Fixed two researchers randomly and the control group,experimental group into literature alone assessed,Use software Review Manager 5.3 analyze aspirin and clopidogrel,aspirin and ticagrelor drug for the safety of the drug after therapy intervention Meta analysis,merge effect value odds ratio(OR)and 95%confidence interval,inspection heterogeneity and publication bias.Results:a total of 16 articles met the criteria,and 3007 patients were included in the meta-analysis.Compared with the clopidogrel sulfate group,the tigrilol group significantly reduced the incidence of major cardiovascular adverse events after interventional therapy for non-ST-segment elevation acute coronary syndrome(MACE)OR=0.37,95%Cl=(0.280.49)(P<0.00001)、Angina symptoms OR=0.46,95%Cl=(0.260.81)(P=0.008)、Non-fatal heart infarction OR=0.38,95%cl=(0.260.81)(P=0.001)、heart failure OR=0.41,95%cl=(0.180.96)(P=0.04)、cardiac death OR=0.31,95%cl=(0.150.64)(P=0.002)、target vessel revascularization OR=0.46,95%cl=(0.210.99)(P=0.05),However,increased respiratory distress OR=4.44,95%cl=(2.089.45)(P<0.0001)、bleeding events OR=2.06,95%cl=(1.303.27)(P=0.002)、arrhythmia OR=1.14,95%cl=(0.851.54)(P=0.39).Conclusion:In the treatment of non-ST-segment elevation acute coronary syndrome after interventional surgery,compared with clopidogrel,Tigliero can reduce the incidence of major cardiovascular adverse events,angina symptoms,non-fatal cardiac infarction,heart failure,cardiogenic death,and target vessel revascularization,However,the incidence of dyspnea,bleeding events and arrhythmias increased.
基金Supported by National Natural Science Foundation of China(31260061,31060039)Key Laboratory of Special Biological Resource Development and Utilization of Universities in Yunnan Province(GXZD201601)+1 种基金Key Discipline Construction Project of Kunming UniversityNational College Students'Innovation Project of China
文摘[ Objective] This study aimed to screen target genes regulated by heat shock factor AtHsfAla in Arabidopsis thaliana. [ Method] Using AtHsfAla-in- serted mutant athsfala （SALK-068042） and wild-type A. thaliana seedlings as experimental materials, target genes regulated by heat shock factor AtHsfAla were screened by microarray assay. Differentially expressed genes were screened by multiple method. Specific functions of differentially expressed genes were analyzed by gene ontology （GO） analysis. Signal transduction pathways, in which differentia｜ly expressed genes were involved, were analyzed by pathway analysis. Gene-gene interaction network was constructed by Signal-Net. [ Result] A total of 3 672 differentially expressed genes were screened out. Up-regulated differentially expressed genes were involved in 198 functions and 7 signal transduction pathways; down-regulated differentially expressed genes were involved in 94 functions and 10 signal transduction pathways. In the signal transduction network, it was found that cwlNV4 and HXK3 had relatively high ability of mediation; AT1 G14240 and cwlNV4 ex- hibited the most interactions with other genes, which were located in key positions throughout the gene-gene interaction network. [ Conclusion] Heat shock factor AtHsfAla regulates a large number of target genes in A. thaliana.
