This study investigates the relationship between polymorphisms in the cathepsin B(ctsb)gene and body weight in Asian seabass(Lates calcarifer).Despite the economic significance of this species,the genetic factors that...This study investigates the relationship between polymorphisms in the cathepsin B(ctsb)gene and body weight in Asian seabass(Lates calcarifer).Despite the economic significance of this species,the genetic factors that influence growth performance in fish remain poorly understood.CTSB,a lysosomal cysteine protease involved in protein degradation and tissue remodelling,is a crucial regulator of growth.We analysed the full-length cDNA of ctsb and mapped it to the major quantitative trait locus(QTL)for growth on linkage group 2 in L.calcarifer.Single nucleotide polymorphisms(SNPs)within ctsb were identified and their associations with body weight were analysed in 298 individuals.Results revealed that SNP1(C>T)in intron 2 of ctsb was significantly correlated with body weight.Quantitative real-time PCR(qRT-PCR)showed that ctsb was expressed in all tissues,with the highest expression in the gills and kidney of developing seabass.Knockdown/overexpression of ctsb in cell lines respectively promoted/inhibited cell proliferation without altering cell size.These findings suggest that ctsb plays a significant role in growth regulation in Asian seabass.This study provides a potential molecular marker for selecting fast-growing Asian seabass.Future research should focus on identifying causal variants in ctsb associated with accelerated growth,conducting in vivo studies,and exploring related signalling pathways.展开更多
基金supported by the Internal Funds of the Temasek Life Sciences Laboratory(5020),Singapore.
文摘This study investigates the relationship between polymorphisms in the cathepsin B(ctsb)gene and body weight in Asian seabass(Lates calcarifer).Despite the economic significance of this species,the genetic factors that influence growth performance in fish remain poorly understood.CTSB,a lysosomal cysteine protease involved in protein degradation and tissue remodelling,is a crucial regulator of growth.We analysed the full-length cDNA of ctsb and mapped it to the major quantitative trait locus(QTL)for growth on linkage group 2 in L.calcarifer.Single nucleotide polymorphisms(SNPs)within ctsb were identified and their associations with body weight were analysed in 298 individuals.Results revealed that SNP1(C>T)in intron 2 of ctsb was significantly correlated with body weight.Quantitative real-time PCR(qRT-PCR)showed that ctsb was expressed in all tissues,with the highest expression in the gills and kidney of developing seabass.Knockdown/overexpression of ctsb in cell lines respectively promoted/inhibited cell proliferation without altering cell size.These findings suggest that ctsb plays a significant role in growth regulation in Asian seabass.This study provides a potential molecular marker for selecting fast-growing Asian seabass.Future research should focus on identifying causal variants in ctsb associated with accelerated growth,conducting in vivo studies,and exploring related signalling pathways.
