Background:We aimed to characterize the protective effects and the molecular mechanisms of action of a Saccharomyces cerevisiae fermentation product(NTK)in response to a mastitis challenge.Eighteen mid-lactation multi...Background:We aimed to characterize the protective effects and the molecular mechanisms of action of a Saccharomyces cerevisiae fermentation product(NTK)in response to a mastitis challenge.Eighteen mid-lactation multiparous Holstein cows(n=9/group)were fed the control diet(CON)or CON supplemented with 19 g/d NTK for 45 d(phase 1,P1)and then infected in the right rear quarter with 2500 CFU of Streptococcus uberis(phase 2,P2).After 36-h,mammary gland and liver biopsies were collected and antibiotic treatment started until the end of P2(9 d post challenge).Cows were then followed until day 75(phase 3,P3).Milk yield(MY)and dry matter intake(DMI)were recorded daily.Milk samples for somatic cell score were collected,and rectal and udder temperature,heart and respiration rate were recorded during the challenge period(P2)together with blood samples for metabolite and immune function analyses.Data were analyzed by phase using the PROC MIXED procedure in SAS.Biopsies were used for transcriptomic analysis via RNA-sequencing,followed by pathway analysis.Results:DMI and MY were not affected by diet in P1,but an interaction with time was recorded in P2 indicating a better recovery from the challenge in NTK compared with CON.NTK reduced rectal temperature,somatic cell score,and temperature of the infected quarter during the challenge.Transcriptome data supported these findings,as NTK supplementation upregulated mammary genes related to immune cell antibacterial function(e.g.,CATHL4,NOS2),epithelial tissue protection(e.g.IL17C),and anti-inflammatory activity(e.g.,ATF3,BAG3,IER3,G-CSF,GRO1,ZFAND2A).Pathway analysis indicated upregulation of tumor necrosis factorα,heat shock protein response,and p21 related pathways in the response to mastitis in NTK cows.Other pathways for detoxification and cytoprotection functions along with the tight junction pathway were also upregulated in NTK-fed cows.Conclusions:Overall,results highlighted molecular networks involved in the protective effect of NTK prophylactic supplementation on udder health during a subclinical mastitic event.展开更多
In 2018,the STAR collaboration collected data from^(96)_(44)Ru+^(96)_(44)Ru and^(96)_(40)Zr+^(96)_(40)Zr at√^(S)NN=200 Ge V to search for the presence of the chiral magnetic effect in collisions of nuclei.The isobar ...In 2018,the STAR collaboration collected data from^(96)_(44)Ru+^(96)_(44)Ru and^(96)_(40)Zr+^(96)_(40)Zr at√^(S)NN=200 Ge V to search for the presence of the chiral magnetic effect in collisions of nuclei.The isobar collision species alternated frequently between 9644 Ru+^(96)_(44)Ru and^(96)_(40)Zr+^(96)_(40)Zr.In order to conduct blind analyses of studies related to the chiral magnetic effect in these isobar data,STAR developed a three-step blind analysis procedure.Analysts are initially provided a"reference sample"of data,comprised of a mix of events from the two species,the order of which respects time-dependent changes in run conditions.After tuning analysis codes and performing time-dependent quality assurance on the reference sample,analysts are provided a species-blind sample suitable for calculating efficiencies and corrections for individual≈30-min data-taking runs.For this sample,species-specific information is disguised,but individual output files contain data from a single isobar species.Only run-by-run corrections and code alteration subsequent to these corrections are allowed at this stage.Following these modifications,the"frozen"code is passed over the fully un-blind data,completing the blind analysis.As a check of the feasibility of the blind analysis procedure,analysts completed a"mock data challenge,"analyzing data from Au+Au collisions at√^(S)NN=27 Ge V,collected in 2018.The Au+Au data were prepared in the same manner intended for the isobar blind data.The details of the blind analysis procedure and results from the mock data challenge are presented.展开更多
High transverse momentum(pT)particle production is suppressed owing to the parton(jet)energy loss in the hot dense medium created in relativistic heavy-ion collisions.Redistribution of energy at low-to-modest pT has b...High transverse momentum(pT)particle production is suppressed owing to the parton(jet)energy loss in the hot dense medium created in relativistic heavy-ion collisions.Redistribution of energy at low-to-modest pT has been difficult to measure,owing to large anisotropic backgrounds.We report a data-driven method for background evaluation and subtraction,exploiting the away-side pseudorapidity gaps,to measure the jetlike correlation shape in Au+Au collisions at √sNN=200 GeV in the STAR experiment.The correlation shapes,for trigger particles pT>3GeV/c and various associated particle pT ranges within 0.5<pT<10GeV/c,are consistent with Gaussians,and their widths increase with centrality.The results indicate jet broadening in the medium created in central heavy-ion collisions.展开更多
Boldenone is a protein-assimilating androgen steroid that can promote protein synthesis,support nitrogen storage,and enhance renal erythropoietin release.The industrial production of boldenone mainly relies on chemica...Boldenone is a protein-assimilating androgen steroid that can promote protein synthesis,support nitrogen storage,and enhance renal erythropoietin release.The industrial production of boldenone mainly relies on chemical synthesis,which has various problems,such as a complex conversion process,excessive byproducts,and serious environmental pollution.There-fore,it is of great significance to explore a new biosynthetic route.Recently,the enzymatic synthesis of steroid compounds has been performed more frequently than in the past.In this work,boldenone was produced from androstenedione(AD)in two steps by a dual-enzyme cascade of 17β-hydroxysteroid dehydrogenase(17β-HSD)and 3-sterone-Δ^(1)-dehydrogenase(KstD).The conversion efficiency of three isoenzymes of 17β-HSD from Mycobacterium sp.LY-1 for substrate AD was first analyzed.After that,the 17β-HSD2 with high selectivity and specificity for AD was screened and co-expressed with KstD3 in Escherichia coli BL21 to construct a dual-enzyme catalytic system.The results showed that the synthesis of boldenone from AD could be achieved by constructing the dual-enzyme expression system of 17β-HSD and KstD,as we determined that the concentration of boldenone reached 24.3 mg/L.To further improve the synthesis efficiency of boldenone,the expression conditions of the dual-enzyme system were optimized,and the concentration of boldenone reached 31.9 mg/L.The explora-tion of this route will provide a foundation for the efficient enzymatic synthesis of boldenone.展开更多
文摘Background:We aimed to characterize the protective effects and the molecular mechanisms of action of a Saccharomyces cerevisiae fermentation product(NTK)in response to a mastitis challenge.Eighteen mid-lactation multiparous Holstein cows(n=9/group)were fed the control diet(CON)or CON supplemented with 19 g/d NTK for 45 d(phase 1,P1)and then infected in the right rear quarter with 2500 CFU of Streptococcus uberis(phase 2,P2).After 36-h,mammary gland and liver biopsies were collected and antibiotic treatment started until the end of P2(9 d post challenge).Cows were then followed until day 75(phase 3,P3).Milk yield(MY)and dry matter intake(DMI)were recorded daily.Milk samples for somatic cell score were collected,and rectal and udder temperature,heart and respiration rate were recorded during the challenge period(P2)together with blood samples for metabolite and immune function analyses.Data were analyzed by phase using the PROC MIXED procedure in SAS.Biopsies were used for transcriptomic analysis via RNA-sequencing,followed by pathway analysis.Results:DMI and MY were not affected by diet in P1,but an interaction with time was recorded in P2 indicating a better recovery from the challenge in NTK compared with CON.NTK reduced rectal temperature,somatic cell score,and temperature of the infected quarter during the challenge.Transcriptome data supported these findings,as NTK supplementation upregulated mammary genes related to immune cell antibacterial function(e.g.,CATHL4,NOS2),epithelial tissue protection(e.g.IL17C),and anti-inflammatory activity(e.g.,ATF3,BAG3,IER3,G-CSF,GRO1,ZFAND2A).Pathway analysis indicated upregulation of tumor necrosis factorα,heat shock protein response,and p21 related pathways in the response to mastitis in NTK cows.Other pathways for detoxification and cytoprotection functions along with the tight junction pathway were also upregulated in NTK-fed cows.Conclusions:Overall,results highlighted molecular networks involved in the protective effect of NTK prophylactic supplementation on udder health during a subclinical mastitic event.
文摘In 2018,the STAR collaboration collected data from^(96)_(44)Ru+^(96)_(44)Ru and^(96)_(40)Zr+^(96)_(40)Zr at√^(S)NN=200 Ge V to search for the presence of the chiral magnetic effect in collisions of nuclei.The isobar collision species alternated frequently between 9644 Ru+^(96)_(44)Ru and^(96)_(40)Zr+^(96)_(40)Zr.In order to conduct blind analyses of studies related to the chiral magnetic effect in these isobar data,STAR developed a three-step blind analysis procedure.Analysts are initially provided a"reference sample"of data,comprised of a mix of events from the two species,the order of which respects time-dependent changes in run conditions.After tuning analysis codes and performing time-dependent quality assurance on the reference sample,analysts are provided a species-blind sample suitable for calculating efficiencies and corrections for individual≈30-min data-taking runs.For this sample,species-specific information is disguised,but individual output files contain data from a single isobar species.Only run-by-run corrections and code alteration subsequent to these corrections are allowed at this stage.Following these modifications,the"frozen"code is passed over the fully un-blind data,completing the blind analysis.As a check of the feasibility of the blind analysis procedure,analysts completed a"mock data challenge,"analyzing data from Au+Au collisions at√^(S)NN=27 Ge V,collected in 2018.The Au+Au data were prepared in the same manner intended for the isobar blind data.The details of the blind analysis procedure and results from the mock data challenge are presented.
文摘High transverse momentum(pT)particle production is suppressed owing to the parton(jet)energy loss in the hot dense medium created in relativistic heavy-ion collisions.Redistribution of energy at low-to-modest pT has been difficult to measure,owing to large anisotropic backgrounds.We report a data-driven method for background evaluation and subtraction,exploiting the away-side pseudorapidity gaps,to measure the jetlike correlation shape in Au+Au collisions at √sNN=200 GeV in the STAR experiment.The correlation shapes,for trigger particles pT>3GeV/c and various associated particle pT ranges within 0.5<pT<10GeV/c,are consistent with Gaussians,and their widths increase with centrality.The results indicate jet broadening in the medium created in central heavy-ion collisions.
基金supported by National Key Research and Development Program of China(No.2019YFA0905300)the National Natural Science Foundation of China(No.22078126)+1 种基金Qing Lan Project in Jiangsu ProvinceFundamental Research Funds for Central Universities of China(No.JUSRP221025).
文摘Boldenone is a protein-assimilating androgen steroid that can promote protein synthesis,support nitrogen storage,and enhance renal erythropoietin release.The industrial production of boldenone mainly relies on chemical synthesis,which has various problems,such as a complex conversion process,excessive byproducts,and serious environmental pollution.There-fore,it is of great significance to explore a new biosynthetic route.Recently,the enzymatic synthesis of steroid compounds has been performed more frequently than in the past.In this work,boldenone was produced from androstenedione(AD)in two steps by a dual-enzyme cascade of 17β-hydroxysteroid dehydrogenase(17β-HSD)and 3-sterone-Δ^(1)-dehydrogenase(KstD).The conversion efficiency of three isoenzymes of 17β-HSD from Mycobacterium sp.LY-1 for substrate AD was first analyzed.After that,the 17β-HSD2 with high selectivity and specificity for AD was screened and co-expressed with KstD3 in Escherichia coli BL21 to construct a dual-enzyme catalytic system.The results showed that the synthesis of boldenone from AD could be achieved by constructing the dual-enzyme expression system of 17β-HSD and KstD,as we determined that the concentration of boldenone reached 24.3 mg/L.To further improve the synthesis efficiency of boldenone,the expression conditions of the dual-enzyme system were optimized,and the concentration of boldenone reached 31.9 mg/L.The explora-tion of this route will provide a foundation for the efficient enzymatic synthesis of boldenone.
