The imbalance of nitric oxide(NO)homeostasis in the brain is closely related to the occurrence of Parkinson’s disease(PD).Therefore,revealing the fluctuation of NO in brain is crucial for understanding the pathophysi...The imbalance of nitric oxide(NO)homeostasis in the brain is closely related to the occurrence of Parkinson’s disease(PD).Therefore,revealing the fluctuation of NO in brain is crucial for understanding the pathophysiological processes.However,currently developed NO probes are unsuitable for this purpose due to their poor blood-brain barrier permeability.Herein,a fluorescent probe(PO-NH)with blood-brain barrier crossing capability and high selectivity for NO was developed.Under the NO mediation,the photoinduced electron transfer(PET)process of the probe was blocked,giving an intensive fluorescence enhancement(F/F_(0)=15).Moreover,PO-NH can be used to effectively monitor changes in intracellular NO levels.Significantly,due to excellent blood-brain barrier crossing ability and near-infrared(NIR)emission,PO-NH is suitable for in vivo imaging of NO in the brain and illustrating with the deterioration of PD,the level of NO gradually increased in the brain of PD mice.We believe that PO-NH may provide a beneficial tool for understanding the biological function of NO in the brain and revealing the complex connection between NO and PD.展开更多
Long-wavelength fluorescent dyes have revolutionized in vivo fluorescence imaging by offering unparalleled spatial resolution.Nevertheless,the majority of organic fluorescent dyes documented to date exhibit emission w...Long-wavelength fluorescent dyes have revolutionized in vivo fluorescence imaging by offering unparalleled spatial resolution.Nevertheless,the majority of organic fluorescent dyes documented to date exhibit emission wavelengths predominantly within the range of 1000-1100 nm,with scarcely any surpassing the 1200 nm threshold.Herein,we introduce a heptamethine cyanine dye that boasts a remarkable fluorescence wavelength of 1239 nm.To our knowledge,this represents the longest fluorescence wavelength for heptamethine benzindole derivative.We have meticulously analyzed its photophysical characteristics and solvatochromic behavior,and assessed its efficacy in vivo fluorescence imaging applications.展开更多
基金the Program of Graduate Education and Teaching Reform of Shanxi(No.2022YJJG302)the Applied Basic Research Programs of Shanxi(Nos.201801D221106,202203021221228)+1 种基金the Key R&D Project of Lvliang(No.2023GXYF04)Scientific Instrument Center of Shanxi University(No.201512)。
文摘The imbalance of nitric oxide(NO)homeostasis in the brain is closely related to the occurrence of Parkinson’s disease(PD).Therefore,revealing the fluctuation of NO in brain is crucial for understanding the pathophysiological processes.However,currently developed NO probes are unsuitable for this purpose due to their poor blood-brain barrier permeability.Herein,a fluorescent probe(PO-NH)with blood-brain barrier crossing capability and high selectivity for NO was developed.Under the NO mediation,the photoinduced electron transfer(PET)process of the probe was blocked,giving an intensive fluorescence enhancement(F/F_(0)=15).Moreover,PO-NH can be used to effectively monitor changes in intracellular NO levels.Significantly,due to excellent blood-brain barrier crossing ability and near-infrared(NIR)emission,PO-NH is suitable for in vivo imaging of NO in the brain and illustrating with the deterioration of PD,the level of NO gradually increased in the brain of PD mice.We believe that PO-NH may provide a beneficial tool for understanding the biological function of NO in the brain and revealing the complex connection between NO and PD.
基金supported by the National Natural Science Foundation of China(No.22277069)Fundamental Research Program of Shanxi Province(Nos.202303021222134,202303021224003)+2 种基金Open Project Fund from Key Laboratory of Coal Environmental Pathogenicity and Prevention(Shanxi Medical University),Ministry of Education,China(No.MEKLCEPP/SXMU-202414)Doctor Fund Project of Shanxi Province(No.SD2324)Doctor Fund Project of Shanxi Medical University(No.XD2222)。
文摘Long-wavelength fluorescent dyes have revolutionized in vivo fluorescence imaging by offering unparalleled spatial resolution.Nevertheless,the majority of organic fluorescent dyes documented to date exhibit emission wavelengths predominantly within the range of 1000-1100 nm,with scarcely any surpassing the 1200 nm threshold.Herein,we introduce a heptamethine cyanine dye that boasts a remarkable fluorescence wavelength of 1239 nm.To our knowledge,this represents the longest fluorescence wavelength for heptamethine benzindole derivative.We have meticulously analyzed its photophysical characteristics and solvatochromic behavior,and assessed its efficacy in vivo fluorescence imaging applications.
