The imbalance of nitric oxide(NO)homeostasis in the brain is closely related to the occurrence of Parkinson’s disease(PD).Therefore,revealing the fluctuation of NO in brain is crucial for understanding the pathophysi...The imbalance of nitric oxide(NO)homeostasis in the brain is closely related to the occurrence of Parkinson’s disease(PD).Therefore,revealing the fluctuation of NO in brain is crucial for understanding the pathophysiological processes.However,currently developed NO probes are unsuitable for this purpose due to their poor blood-brain barrier permeability.Herein,a fluorescent probe(PO-NH)with blood-brain barrier crossing capability and high selectivity for NO was developed.Under the NO mediation,the photoinduced electron transfer(PET)process of the probe was blocked,giving an intensive fluorescence enhancement(F/F_(0)=15).Moreover,PO-NH can be used to effectively monitor changes in intracellular NO levels.Significantly,due to excellent blood-brain barrier crossing ability and near-infrared(NIR)emission,PO-NH is suitable for in vivo imaging of NO in the brain and illustrating with the deterioration of PD,the level of NO gradually increased in the brain of PD mice.We believe that PO-NH may provide a beneficial tool for understanding the biological function of NO in the brain and revealing the complex connection between NO and PD.展开更多
基金the Program of Graduate Education and Teaching Reform of Shanxi(No.2022YJJG302)the Applied Basic Research Programs of Shanxi(Nos.201801D221106,202203021221228)+1 种基金the Key R&D Project of Lvliang(No.2023GXYF04)Scientific Instrument Center of Shanxi University(No.201512)。
文摘The imbalance of nitric oxide(NO)homeostasis in the brain is closely related to the occurrence of Parkinson’s disease(PD).Therefore,revealing the fluctuation of NO in brain is crucial for understanding the pathophysiological processes.However,currently developed NO probes are unsuitable for this purpose due to their poor blood-brain barrier permeability.Herein,a fluorescent probe(PO-NH)with blood-brain barrier crossing capability and high selectivity for NO was developed.Under the NO mediation,the photoinduced electron transfer(PET)process of the probe was blocked,giving an intensive fluorescence enhancement(F/F_(0)=15).Moreover,PO-NH can be used to effectively monitor changes in intracellular NO levels.Significantly,due to excellent blood-brain barrier crossing ability and near-infrared(NIR)emission,PO-NH is suitable for in vivo imaging of NO in the brain and illustrating with the deterioration of PD,the level of NO gradually increased in the brain of PD mice.We believe that PO-NH may provide a beneficial tool for understanding the biological function of NO in the brain and revealing the complex connection between NO and PD.
