The highly selective hydrogenation of 5-hydroxymethylfurfural to 2,5-dihydroxymethylfuran is an important reaction in the field of biomass hydrogenation,because it is a bridge between biomass resources and chemical in...The highly selective hydrogenation of 5-hydroxymethylfurfural to 2,5-dihydroxymethylfuran is an important reaction in the field of biomass hydrogenation,because it is a bridge between biomass resources and chemical industry.Here,we precisely constructed carbon nitride supported Pd-based catalysts by a simple impregnation-reduction method.By changing the reduction temperature,catalysts with different oxidation state could be precisely constructed.Moreover,the important correlation between the ratio of Pd^(0)/Pd^(2+)and catalytic activity is revealed during the selective hydrogenation of HMF.The Pd/g—C_(3)N_(4)—300 catalyst with a Pd^(0)/Pd^(2+)ratio of 3/2 showed the highest catalytic activity,which could get 96.9%5-hydroxymethylfurfural conversion and 90.3%2,5-dihydroxymethylfuran selectivity.Further density functional theory calculation revealed that the synergistic effect between Pd0and Pd2+in Pd/g—C_(3)N_(4)—300 system could boost the adsorption of the substrate and the dissociation of hydrogen.In this work,we highlight the important correlation between metal oxidation state and catalytic activity,which provides valuable insights for the rational design of precious metal catalysts for hydrogenation reactions.展开更多
Background and aims:Hepatocellular carcinoma is one of the most common cancers worldwide.Previous studies have reported he influence of various hepatic blood flow occlusions on tumor behavior,which is mainly mediated ...Background and aims:Hepatocellular carcinoma is one of the most common cancers worldwide.Previous studies have reported he influence of various hepatic blood flow occlusions on tumor behavior,which is mainly mediated by liver ischemia-reperfusion.Although some genes and pathways have been determined,the whole transcriptome after various hepatic blood flow occlusions is lacking.Methods:We systematically explored transcriptome changes after various hepatic blood flow occlusions,including sham operation(SO;n=10),occlusion of the portal triad(OPT;n=10),and occlusion of the portal vein(OPV;n=10),by RNA-sequencing.Results:HE sections and TUNEL assays showed different liver injury among groups.We identified the top altered genes and pathways.Compared with the SO group,96 genes were altered in OPV,with 81 upregulated and 15 downregulated genes.The top 5 upregulated genes were Pdk4,Serpina12,Depp1,Igfbp1,and Mup22.The top 5 downregulated genes were Sprr1a,Serpinb2,Tnc,Cdkn3,and Cenpu.Compared with the SO group,there were 20 differentially expressed genes in OPT,with 18 upregulated and 2 downregulated genes.The top 5 upregulated genes wereC7,Zbtb16,Gabrp,Pdk4,and Mmrn1.The top 2 downregulated genes were Krt20 and Sis.Compared with the OPV group,72 differentially expressed genes were in OPT,with 39 upregulated and 33 downregulated genes.The top 5 upregulated genes wereHspa1b,Hbb-bs,Phf19,Ddias,and Rad54b.The top 5 downregulated genes were Cish,Socs2,Slc25a30,Rgs3,and Hsd3b5.Conclusion:Various surgical methods have an obvious influence on the transcriptome of tumors.展开更多
Histopathology plays a great role in diagnosing various diseases,which is considered as a golden standard for tumor identification.The tissue constituents must be stained by visible labels for microscopic analysis by ...Histopathology plays a great role in diagnosing various diseases,which is considered as a golden standard for tumor identification.The tissue constituents must be stained by visible labels for microscopic analysis by medical experts.However,this process is time-consuming,labor-intensive,and expensive,which requires rapid pathological approaches for diagnosis in the operating room.Here,we present an easy-to-process and high-performance perovskite biological probes for rapid and visual pathological diagnosis of glioma.Perovskite quantum dots can be encapsulated by the copolymer into nanocrystals(PNCs)with a diameter of 100 nm,which is modified with chlorotoxin to achieve the specific recognition of glioma.Benefiting from the super photoluminescence quantum yield(above 93%)of EVA@PNCs aqueous solution,the glioma can be clearly imaged and captured via a smartphone under the excitation of a handheld UV lamp.To demonstrate the visualization and efficiency of PNC probes,different malignant grades of brain tumor sections can be distinguished in no more than 5 min.This strategy provides a general auxiliary diagnosis platform for achieving the histopathology analysis near the operating bed,which is currently not feasible with standard histochemical staining methods.展开更多
基金supported by the National Key Research and Development Program of China(2021YFA1500500)。
文摘The highly selective hydrogenation of 5-hydroxymethylfurfural to 2,5-dihydroxymethylfuran is an important reaction in the field of biomass hydrogenation,because it is a bridge between biomass resources and chemical industry.Here,we precisely constructed carbon nitride supported Pd-based catalysts by a simple impregnation-reduction method.By changing the reduction temperature,catalysts with different oxidation state could be precisely constructed.Moreover,the important correlation between the ratio of Pd^(0)/Pd^(2+)and catalytic activity is revealed during the selective hydrogenation of HMF.The Pd/g—C_(3)N_(4)—300 catalyst with a Pd^(0)/Pd^(2+)ratio of 3/2 showed the highest catalytic activity,which could get 96.9%5-hydroxymethylfurfural conversion and 90.3%2,5-dihydroxymethylfuran selectivity.Further density functional theory calculation revealed that the synergistic effect between Pd0and Pd2+in Pd/g—C_(3)N_(4)—300 system could boost the adsorption of the substrate and the dissociation of hydrogen.In this work,we highlight the important correlation between metal oxidation state and catalytic activity,which provides valuable insights for the rational design of precious metal catalysts for hydrogenation reactions.
基金supported by the Beijing Natural Science Foundation of China(7202235).
文摘Background and aims:Hepatocellular carcinoma is one of the most common cancers worldwide.Previous studies have reported he influence of various hepatic blood flow occlusions on tumor behavior,which is mainly mediated by liver ischemia-reperfusion.Although some genes and pathways have been determined,the whole transcriptome after various hepatic blood flow occlusions is lacking.Methods:We systematically explored transcriptome changes after various hepatic blood flow occlusions,including sham operation(SO;n=10),occlusion of the portal triad(OPT;n=10),and occlusion of the portal vein(OPV;n=10),by RNA-sequencing.Results:HE sections and TUNEL assays showed different liver injury among groups.We identified the top altered genes and pathways.Compared with the SO group,96 genes were altered in OPV,with 81 upregulated and 15 downregulated genes.The top 5 upregulated genes were Pdk4,Serpina12,Depp1,Igfbp1,and Mup22.The top 5 downregulated genes were Sprr1a,Serpinb2,Tnc,Cdkn3,and Cenpu.Compared with the SO group,there were 20 differentially expressed genes in OPT,with 18 upregulated and 2 downregulated genes.The top 5 upregulated genes wereC7,Zbtb16,Gabrp,Pdk4,and Mmrn1.The top 2 downregulated genes were Krt20 and Sis.Compared with the OPV group,72 differentially expressed genes were in OPT,with 39 upregulated and 33 downregulated genes.The top 5 upregulated genes wereHspa1b,Hbb-bs,Phf19,Ddias,and Rad54b.The top 5 downregulated genes were Cish,Socs2,Slc25a30,Rgs3,and Hsd3b5.Conclusion:Various surgical methods have an obvious influence on the transcriptome of tumors.
基金National Natural Science Foundation of China,Grant/Award Numbers:52222313,22075296,91963212,32271108Beijing Municipal Science and Technology Commission,Grant/Award Numbers:Z201100006820037,Z211100002121001+1 种基金Youth Innovation Promotion Association CAS,Grant/Award Number:2020032Beijing National Laboratory for Molecular Sciences,Grant/Award Number:BNLMSCXXM-202005。
文摘Histopathology plays a great role in diagnosing various diseases,which is considered as a golden standard for tumor identification.The tissue constituents must be stained by visible labels for microscopic analysis by medical experts.However,this process is time-consuming,labor-intensive,and expensive,which requires rapid pathological approaches for diagnosis in the operating room.Here,we present an easy-to-process and high-performance perovskite biological probes for rapid and visual pathological diagnosis of glioma.Perovskite quantum dots can be encapsulated by the copolymer into nanocrystals(PNCs)with a diameter of 100 nm,which is modified with chlorotoxin to achieve the specific recognition of glioma.Benefiting from the super photoluminescence quantum yield(above 93%)of EVA@PNCs aqueous solution,the glioma can be clearly imaged and captured via a smartphone under the excitation of a handheld UV lamp.To demonstrate the visualization and efficiency of PNC probes,different malignant grades of brain tumor sections can be distinguished in no more than 5 min.This strategy provides a general auxiliary diagnosis platform for achieving the histopathology analysis near the operating bed,which is currently not feasible with standard histochemical staining methods.
