Viruses,the most diverse of all biological entities,are ubiquitous and can influence nearly all life forms on Earth[1].The preliminary pathway through which viruses affect microbialmetabolism and evolution is viral in...Viruses,the most diverse of all biological entities,are ubiquitous and can influence nearly all life forms on Earth[1].The preliminary pathway through which viruses affect microbialmetabolism and evolution is viral infection[2].There are two distinct infection modes for viruses interacting with prokaryotes,namely temperate and virulent lifestyles[3].Virulent viruses trigger their hosts’death by lysis once they invade the host cells[2],whereas temperate viruses can replicate alongside their host inthe lysogenic cycle until a lytic cycle is induced[2,3].It has beenreported that viral lifestyles are key determinants of virusencoded auxiliary metabolic genes(AMGs)that are crucial toolboxes for viruses to reprogram host metabolisms[4].An in-depthinvestigation of virus-microbe infection modes over a broad geographical scale is thus vital for understanding the potential effectsof viruses on the microbial community and biogeochemical cycles.展开更多
Postharvest seafood mushroom(Hypsizygus marmoreus)is highly susceptible to rapid dehydration,decay,and softening,rendering single preservation technologies insufficient to effectively address these issues.This study i...Postharvest seafood mushroom(Hypsizygus marmoreus)is highly susceptible to rapid dehydration,decay,and softening,rendering single preservation technologies insufficient to effectively address these issues.This study investigated a complementary strategy by integrating a low-voltage electrostatic field(LVEF)with biodegradable poly(butylene adipate-co-terephthalate)/polylactic acid(PBAT/PLA)packaging to extend the mushroom's shelf life.Results showed that compared with conventional low-density polyethylene(LDPE),PBAT/PLA film effec-tively prevented moisture condensation and maintained lower CO_(2)concentrations.In contrast,LVEF treatment could suppress respiratory intensity,modulate relevant enzyme activity,and significantly mitigate excessive moisture loss caused by PBAT/PLA films.The findings indicated that the combined treatment effectively increased the mushrooms'antioxidant capacity,maintained cell membrane stability,sustained cell wall poly-saccharide levels,and preserved optimal appearance and texture during storage.The combined application of LVEF and PBAT/PLA packaging effectively preserved quality and extended the shelf life at 4℃from 6 to 15 days,offering a promising,eco-friendly strategy for preserving high-respiration edible fungi.展开更多
基金supported by the National Key Research and Development Program of China(2022YFF0801901 and 2022YFF0801903)the National Natural Science Foundation of China(32425004)the New Cornerstone Science Foundation through the XPLORER PRIZE.
文摘Viruses,the most diverse of all biological entities,are ubiquitous and can influence nearly all life forms on Earth[1].The preliminary pathway through which viruses affect microbialmetabolism and evolution is viral infection[2].There are two distinct infection modes for viruses interacting with prokaryotes,namely temperate and virulent lifestyles[3].Virulent viruses trigger their hosts’death by lysis once they invade the host cells[2],whereas temperate viruses can replicate alongside their host inthe lysogenic cycle until a lytic cycle is induced[2,3].It has beenreported that viral lifestyles are key determinants of virusencoded auxiliary metabolic genes(AMGs)that are crucial toolboxes for viruses to reprogram host metabolisms[4].An in-depthinvestigation of virus-microbe infection modes over a broad geographical scale is thus vital for understanding the potential effectsof viruses on the microbial community and biogeochemical cycles.
基金funded by the National Key Research and Development Program of China(2022YFA1203604 and 2023YFF1105200)the Postgraduate Research&Practice Innovation Program of Jiangsu Province(KYCX24_2507).
文摘Postharvest seafood mushroom(Hypsizygus marmoreus)is highly susceptible to rapid dehydration,decay,and softening,rendering single preservation technologies insufficient to effectively address these issues.This study investigated a complementary strategy by integrating a low-voltage electrostatic field(LVEF)with biodegradable poly(butylene adipate-co-terephthalate)/polylactic acid(PBAT/PLA)packaging to extend the mushroom's shelf life.Results showed that compared with conventional low-density polyethylene(LDPE),PBAT/PLA film effec-tively prevented moisture condensation and maintained lower CO_(2)concentrations.In contrast,LVEF treatment could suppress respiratory intensity,modulate relevant enzyme activity,and significantly mitigate excessive moisture loss caused by PBAT/PLA films.The findings indicated that the combined treatment effectively increased the mushrooms'antioxidant capacity,maintained cell membrane stability,sustained cell wall poly-saccharide levels,and preserved optimal appearance and texture during storage.The combined application of LVEF and PBAT/PLA packaging effectively preserved quality and extended the shelf life at 4℃from 6 to 15 days,offering a promising,eco-friendly strategy for preserving high-respiration edible fungi.