Patulin is a mycotoxin that widely contaminates fruits and their derivative products,posing a significant threat to global food safety and human health.Recent years have seen an increase in research focused on patulin...Patulin is a mycotoxin that widely contaminates fruits and their derivative products,posing a significant threat to global food safety and human health.Recent years have seen an increase in research focused on patulin removal,with biological methods emerging as the most effective and safe solutions.This study utilized the previously identified critical degrading enzyme,PgSDR-A5D9S1,which was extracted and purified,and applied it to juice and apple systems.Through density functional energy calculation simulations,we predicted the critical site,SER-256,that is involved in the interaction between the degrading enzyme and patulin.The experimental results demonstrated that the concentration of 100μg/L PgSDR-A5D9S1 degrading enzyme achieved degradation rates of 75%and 35%for patulin in juice and apple systems in 6 h,respectively.Furthermore,the enzyme exhibited significant inhibitory effects on Penicillium in the apple system,effectively preventing food contamination by patulin.展开更多
基金supported by the National Natural Science Foun-dation of China(grant number 32102107)College and university talents service enterprise project of Xi’an(grant number 2023JH-GXRC-4150127)+1 种基金the Shaanxi Province key industry innovation chain project(2023-ZDLNY-39)the Shaanxi Province key core technology research projects(2024NC-GJHX-06).
文摘Patulin is a mycotoxin that widely contaminates fruits and their derivative products,posing a significant threat to global food safety and human health.Recent years have seen an increase in research focused on patulin removal,with biological methods emerging as the most effective and safe solutions.This study utilized the previously identified critical degrading enzyme,PgSDR-A5D9S1,which was extracted and purified,and applied it to juice and apple systems.Through density functional energy calculation simulations,we predicted the critical site,SER-256,that is involved in the interaction between the degrading enzyme and patulin.The experimental results demonstrated that the concentration of 100μg/L PgSDR-A5D9S1 degrading enzyme achieved degradation rates of 75%and 35%for patulin in juice and apple systems in 6 h,respectively.Furthermore,the enzyme exhibited significant inhibitory effects on Penicillium in the apple system,effectively preventing food contamination by patulin.
