[Objective] This study aimed to make a survey of domestic pigeons for infection with Eimeria species of coccidia was conducted in Guangdong Province, southern China. [Method] A total of 244 fecal samples(102 from Huiz...[Objective] This study aimed to make a survey of domestic pigeons for infection with Eimeria species of coccidia was conducted in Guangdong Province, southern China. [Method] A total of 244 fecal samples(102 from Huizhou, 83 from Jiangmen, 35 from Chaozhou and 24 from Guangzhou, respectively) in four pigeon breeding farms between June 2012 and March2013 were collected and examined microscopically. [Results] Eimeria oocysts were seen in 223(91.4%) fecal samples, with three species, namely E. labbeana, E.columbae and E. kapotei. E. labbeana was the most common species in Guangdong provinces with an overall prevalence of 91.4%, while a slightly lower incidence of E. columbae and E. kapotei species was detected with11.1% and 6.6%, representatively. The prevalence in different months varied ranging from 83.3% to 91.7%, with the highest prevalence in summer. Nestling groups showed obviously high infection than adult pigeons. [Conclusion] The present survey indicated the wide and severe prevalence of Eimeria infection in Guangdong domestic pigeons, which suggested that integrated measures should be taken to control and prevent coccidiosis of pigeons in this province.展开更多
Etomidate(ETO),a benzodiazepine commonly used by pregnant women with anxiety disorders,has been increasingly detected in aquatic environments.However,its reproductive toxicity and intergenerational effects have not be...Etomidate(ETO),a benzodiazepine commonly used by pregnant women with anxiety disorders,has been increasingly detected in aquatic environments.However,its reproductive toxicity and intergenerational effects have not been adequately assessed.In this study,adult female fish were employed to investigate the reproductive toxicity after ETO exposure.After 4 weeks of exposure,zebrafish ovaries exhibited polycystic ovary syndrome(PCOS)-like changes,including polycystic ovaries,reduced spawning rates,and elevated androgen levels.Moreover,ETO was transmitted to offspring via maternal ovaries,significantly increasing larval mortality and deformity rates while reducing their locomotor activity.Transcriptomic and metabolomic profiling revealed oxidative stress responses,disrupted unsaturated fatty acid and glutathione metabolism,and damage to ovarian granulosa cells and the zona pellucida,as confirmed by reactive oxygen species(ROS)fluorescence staining.Combined multiomics analysis demonstrated that ETO exposure exacerbated ovarian oxidative stress,impaired the functional structure of ovaries,and disrupted follicular cell maturation via hyperactivation of the mechanistic target of the rapamycin complex 1(mTORC1)pathway.These changes culminated in PCOS-like effects in zebrafish.This study provides critical insights into the mechanisms of ETO-induced reproductive toxicity and transgenerational impacts,offering a foundation for more comprehensive risk assessments and potential interventions.展开更多
基金Support by the National Natural Science Foundation of China(Grant No.31402186 and 31302086)the Pearl River Science and Technology New Star Project(Grant No.2012J2200059 and 2014J2200096)+1 种基金the Dean Foundation of Guangdong Academy of Agricultural Sciences(Grant No.201413)the Science and Technology Plan Projects of Guangdong Province(Grant No.2015A020209075,2013B031500005,2014B070706011 and 2013B060400037)
文摘[Objective] This study aimed to make a survey of domestic pigeons for infection with Eimeria species of coccidia was conducted in Guangdong Province, southern China. [Method] A total of 244 fecal samples(102 from Huizhou, 83 from Jiangmen, 35 from Chaozhou and 24 from Guangzhou, respectively) in four pigeon breeding farms between June 2012 and March2013 were collected and examined microscopically. [Results] Eimeria oocysts were seen in 223(91.4%) fecal samples, with three species, namely E. labbeana, E.columbae and E. kapotei. E. labbeana was the most common species in Guangdong provinces with an overall prevalence of 91.4%, while a slightly lower incidence of E. columbae and E. kapotei species was detected with11.1% and 6.6%, representatively. The prevalence in different months varied ranging from 83.3% to 91.7%, with the highest prevalence in summer. Nestling groups showed obviously high infection than adult pigeons. [Conclusion] The present survey indicated the wide and severe prevalence of Eimeria infection in Guangdong domestic pigeons, which suggested that integrated measures should be taken to control and prevent coccidiosis of pigeons in this province.
基金supported by the National Natural Science Foundation of China(Grants No.22206072 and 82293652)the Science and Technology Program of Guangzhou(2024A04J2742).
文摘Etomidate(ETO),a benzodiazepine commonly used by pregnant women with anxiety disorders,has been increasingly detected in aquatic environments.However,its reproductive toxicity and intergenerational effects have not been adequately assessed.In this study,adult female fish were employed to investigate the reproductive toxicity after ETO exposure.After 4 weeks of exposure,zebrafish ovaries exhibited polycystic ovary syndrome(PCOS)-like changes,including polycystic ovaries,reduced spawning rates,and elevated androgen levels.Moreover,ETO was transmitted to offspring via maternal ovaries,significantly increasing larval mortality and deformity rates while reducing their locomotor activity.Transcriptomic and metabolomic profiling revealed oxidative stress responses,disrupted unsaturated fatty acid and glutathione metabolism,and damage to ovarian granulosa cells and the zona pellucida,as confirmed by reactive oxygen species(ROS)fluorescence staining.Combined multiomics analysis demonstrated that ETO exposure exacerbated ovarian oxidative stress,impaired the functional structure of ovaries,and disrupted follicular cell maturation via hyperactivation of the mechanistic target of the rapamycin complex 1(mTORC1)pathway.These changes culminated in PCOS-like effects in zebrafish.This study provides critical insights into the mechanisms of ETO-induced reproductive toxicity and transgenerational impacts,offering a foundation for more comprehensive risk assessments and potential interventions.
