To investigate the potential of KTIG-MIG coupling welding in improving welding efficiency and quality for medium-thickness Q235B low-carbon steel plates,this study specifically analyzes the microstructural characteris...To investigate the potential of KTIG-MIG coupling welding in improving welding efficiency and quality for medium-thickness Q235B low-carbon steel plates,this study specifically analyzes the microstructural characteristics of three distinct regions in butt joints-the base metal(BM),heat-affected zone(HAZ),and weld metal zone(WMZ)-while simultaneously conducting comprehensive mechanical property testing on the welded joints.The test results of mechanical properties are combined with the data of microstructure analysis.The relationship between mechanical properties and microstructure of the WMZ of the butt joint is found.The results show that the microstructure of weld zone does not change significantly with the increase of heat input,but the grain structure of fusion zone and heat affected zone increases gradually.The tensile properties of welded joints are better than those of base materials under different heat input conditions.展开更多
In vitro toxicological assessment helps explore key fractions of particulate matter(PM)in association with the toxic mechanism.Previous studies mainly discussed the toxicity effects of the water-soluble and organic-so...In vitro toxicological assessment helps explore key fractions of particulate matter(PM)in association with the toxic mechanism.Previous studies mainly discussed the toxicity effects of the water-soluble and organic-soluble fractions of PM.However,the toxicity of insoluble fractions is relatively poorly understood,and the adsorption of proteins is rarely considered.In this work,the formation of protein corona on the surface of insoluble particles during incubation in a culture medium was investigated.It was found that highly abundant proteins in fetal bovine serum were the main components of the protein corona.The adsorbed proteins increased the dispersion stability of insoluble particles.Meanwhile,the leaching concentrations of some metal elements(e.g.,Cu,Zn,and Pb)from PM increased in the presence of proteins.The toxicity effects and potential mechanisms of the PM insoluble particle–protein corona complex on macrophage cells RAW264.7 were discussed.The results revealed that the PM insoluble particle–protein corona complex could influence the phagosome pathway in RAW264.7 cells.Thus,it promoted the intracellular reactive oxygen species generation and induced a greater degree of cell differentiation,significantly altering cell morphology.Consequently,this work sheds new light on the combination of insoluble particles and protein corona in terms of PM cytotoxicity assessment.展开更多
基金supported by the National Natural Science Foundation of China(51605103)Key Projects of Science and Technology Research Plan of Hubei Provincial Department of Education(D20221306)Key Project of Hubei Provincial Science and Technology Department(2020BAB055).
文摘To investigate the potential of KTIG-MIG coupling welding in improving welding efficiency and quality for medium-thickness Q235B low-carbon steel plates,this study specifically analyzes the microstructural characteristics of three distinct regions in butt joints-the base metal(BM),heat-affected zone(HAZ),and weld metal zone(WMZ)-while simultaneously conducting comprehensive mechanical property testing on the welded joints.The test results of mechanical properties are combined with the data of microstructure analysis.The relationship between mechanical properties and microstructure of the WMZ of the butt joint is found.The results show that the microstructure of weld zone does not change significantly with the increase of heat input,but the grain structure of fusion zone and heat affected zone increases gradually.The tensile properties of welded joints are better than those of base materials under different heat input conditions.
基金supported by the National Natural Science Foundation of China(91643105,21874065,22176085).
文摘In vitro toxicological assessment helps explore key fractions of particulate matter(PM)in association with the toxic mechanism.Previous studies mainly discussed the toxicity effects of the water-soluble and organic-soluble fractions of PM.However,the toxicity of insoluble fractions is relatively poorly understood,and the adsorption of proteins is rarely considered.In this work,the formation of protein corona on the surface of insoluble particles during incubation in a culture medium was investigated.It was found that highly abundant proteins in fetal bovine serum were the main components of the protein corona.The adsorbed proteins increased the dispersion stability of insoluble particles.Meanwhile,the leaching concentrations of some metal elements(e.g.,Cu,Zn,and Pb)from PM increased in the presence of proteins.The toxicity effects and potential mechanisms of the PM insoluble particle–protein corona complex on macrophage cells RAW264.7 were discussed.The results revealed that the PM insoluble particle–protein corona complex could influence the phagosome pathway in RAW264.7 cells.Thus,it promoted the intracellular reactive oxygen species generation and induced a greater degree of cell differentiation,significantly altering cell morphology.Consequently,this work sheds new light on the combination of insoluble particles and protein corona in terms of PM cytotoxicity assessment.
