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大肠杆菌FMME-N-26生产琥珀酸的发酵条件优化和放大 被引量:4
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作者 刘佳 唐文秀 +4 位作者 王学明 郭亮 陈修来 高聪 刘立明 《过程工程学报》 CAS CSCD 北大核心 2022年第7期853-862,共10页
琥珀酸(Succinic acid)被认为是白色生物技术生产的最具潜力的大宗化学品之一,在工业上具有广泛的应用。微生物发酵生产琥珀酸具有环境友好和可持续发展等优点,展现出良好的发展前景,但是存在得率低、副产物积累、生产强度低等问题。为... 琥珀酸(Succinic acid)被认为是白色生物技术生产的最具潜力的大宗化学品之一,在工业上具有广泛的应用。微生物发酵生产琥珀酸具有环境友好和可持续发展等优点,展现出良好的发展前景,但是存在得率低、副产物积累、生产强度低等问题。为了实现琥珀酸的高效生产,在3.6 L发酵罐中对E. coli FMME-N-26生产琥珀酸发酵条件和补料策略进行了优化,建立了好氧-厌氧两阶段发酵工艺,最终确定发酵策略为:有氧发酵8 h后转为厌氧发酵,MgCO为pH中和剂,发酵72 h补加抗渗透压保护剂2 mmol/L甜菜碱,厌氧阶段控制葡萄糖浓度为1~5 g/L。优化后发酵72 h,琥珀酸的产量和厌氧阶段得率分别达到119.2 g/L和1.08 g/g葡萄糖(理论得率97%),分别比优化前提高了46.4%和4.8%,副产物乙酸和乳酸仅积累2.37和0.94 g/L,分别比优化前降低了37.1%和49.2%。在1000 L发酵罐中实现中试放大生产,E. coli FMME-N-26生产琥珀酸的产量、得率和生产强度在国内外属于领先水平,为琥珀酸工业化生产奠定了坚实的基础,同时也为其他高价值化学品的生产提供了借鉴。 展开更多
关键词 琥珀酸 大肠杆菌 发酵优化 渗透压 pH中和剂
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Comparison of Two Water Treatment Processes with Activated Aluminum Oxide
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作者 Junling wang xueming wang +1 位作者 Cuimin Feng Sheng Wei 《Journal of Water Resource and Protection》 2015年第11期843-850,共8页
We focused on the need for the phosphorus removal in water plant process. As for surface water plant, the enhancement of phosphorus removal in purification process can raise the biological stability of effluent potabl... We focused on the need for the phosphorus removal in water plant process. As for surface water plant, the enhancement of phosphorus removal in purification process can raise the biological stability of effluent potable water. This study conducted the effect of two water treatment processes for phosphorus removal, including the PAC + AAL (the treatment process using poly aluminum chloride and activated aluminum oxide) and PFS + AAL (the treatment process using poly ferric sulfate and activated aluminum oxide). The aim is to use the adsorption capacity of activated aluminum oxide to increase the removal of phosphorus in filtration process. The result showed that the two processes both can remove the phosphorus concentration to reach below 10 μg/L, and the PAC + AAL is the better process. 展开更多
关键词 POLY ALUMINUM CHLORIDE POLY Ferric SULFATE ACTIVATED ALUMINUM Oxide PHOSPHORUS Removal Surface Water
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文本杂合与零翻译
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作者 王雪明 李澜新 《外国语言与文化》 2020年第2期114-123,共10页
零翻译概念自产生以来就一直在争议中不断发展,在讨论中逐渐形成相关理论表述。梳理性研究指出,零翻译的概念内涵呈缩小趋势,体现为“源语符号原封不动地移译”。这种总体趋向虽有合理成分,但亦存在着对翻译复杂性的简化认识。本文从文... 零翻译概念自产生以来就一直在争议中不断发展,在讨论中逐渐形成相关理论表述。梳理性研究指出,零翻译的概念内涵呈缩小趋势,体现为“源语符号原封不动地移译”。这种总体趋向虽有合理成分,但亦存在着对翻译复杂性的简化认识。本文从文本类型、原文杂合性和翻译方向等层面对零翻译概念进行更为深入的探讨,认为零翻译虽为原形移译,但不能否定译者主观能动性的发挥,仍须遵守译语的语言规范和习惯。 展开更多
关键词 零翻译 文本类型 语言规范 译者能动性
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Visual understanding by mining social media: recent advances and challenges
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作者 xueming wang Zechao LI Jinhui TANG 《Frontiers of Computer Science》 SCIE EI CSCD 2018年第3期406-422,共17页
With the rapid increase in social websites that has dramatically increased the volume of social media, which includes the use of images and videos, visual understanding has attracted great interest in several areas su... With the rapid increase in social websites that has dramatically increased the volume of social media, which includes the use of images and videos, visual understanding has attracted great interest in several areas such as multimedia, computer vision, and pattern recognition. Valuable auxiliary resources available on social websites, such as user-provided tags, aid in the tasks of visual understanding. Therefore, sev- eral methods have been proposed for exploring the auxiliary resources for tag refinement, image retrieval, and media sum- marization. This work conducts a comprehensive survey of recent advances in visual understanding by mining social media in order to discuss their merits and limitations. We then analyze the difficulties and challenges of visual understanding followed by several possible future research directions. 展开更多
关键词 social tag visual understanding visual representation tag refinement image retrieval SUMMARIZATION
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