Objective：MicroRNAs （miRNAs） have potential as diagnostic biomarkers in cancer.Evaluation of the association between miRNA expression patterns and early detection of liver metastasis in colorectal cancer （CRC） ha...Objective：MicroRNAs （miRNAs） have potential as diagnostic biomarkers in cancer.Evaluation of the association between miRNA expression patterns and early detection of liver metastasis in colorectal cancer （CRC） has not been reported.Methods：We investigated the expression of metastasis-associated miRs-31,335,206,141,126,200b,200c,21,Let7a,Let7b and Let7c in localized,liver-metastatic and other organ-metastatic CRC （OM-CRC）.Expressions of target miRNAs in serum were evaluated in 116 consecutive localized CRC （L-CRC）,72 synchronous liver-metastatic CRC （SLM-CRC） and 36 other OM-CRC by quantitative real-time PCR.Results：Seven of 11 tested miRNAs could be detected from serum.Four miRNAs,miR-126,Let-7a,miR141 and miR-21 were identified as metastasis-associated miRNAs.Compared with L-CRC,significant upregulated expression was observed for miR-141 and miR-21 in SLM-CRC and OM-CRC,down-regulated expression was observed for miR-126 in SLM-CRC and OM-CRC,and up-regulated expression of Let-7a in OM-CRC.The receiver operating characteristic （ROC） curve showed serum miR-126 had a cut-off [log10 relative quantity （log10RQ）=--0.2005] with 77.78％ sensitivity and 68.97％ specificity with an area under curve （AUC） of 0.7564,miR-141 had a cut-off （1og10RQ=-0.2285） with 86.11％ sensitivity and 76.11％ specificity with an AUC of 0.8279,and miR-21 had a cut-off （log10RQ=-0.1310） with 73.61％ sensitivity and 66.38％ specificity with an AUC of 0.7479.Conclusions：We identified liver metastasis-associated miRNAs,suggesting serum miR-126,miR-141 and miR-21 may be novel biomarkers for clinical diagnosis of early stage liver-metastatic CRC.展开更多
EBI2 is known as a kind of 7TM G protein-coupled receptors, and EBi2 gene is one of the most upregulated genes in Epstein-Barr virus （EBV） -infected lymphocytes. As a constitutively active receptor, EBI2 signals via...EBI2 is known as a kind of 7TM G protein-coupled receptors, and EBi2 gene is one of the most upregulated genes in Epstein-Barr virus （EBV） -infected lymphocytes. As a constitutively active receptor, EBI2 signals via Gai, and TM-II plays an important role in the signal transduct/on process. EBI2 is expressed mainly in mature, B cells and the expression level increases at the early stage of activation; subsequently, B cells are transferred into GC and the expression level is down-regulated. EBI2 can regulate independently and mediate synergistically the migration of B cell towards the extrafollicular areas and GC depending on chemo- kine receptors CXCR5 and CCR7 （EBII）. Differential expression of EBI2 receptor is conducive to accurate migration of B cells at different stages, thereby leading to appropriate irranunologic responses to T-dependent antigens. Recently, two research groups identified a specific oxysteml as the natural ligand for EBL2. Among all the oxysterols, 7α, 25-Dihydrexycholesteml （7a, 25-OHC） is the most effective ligand and activator, and EBI2-Oxysterol signal pathway plays an important role in adaptive immune responses. In addition, EBI2 is also closely related with specific kinds of autoimmune diseases, so it is considered as a negative regulator of the innate immune response in macrophages.展开更多
M13 phage＇is a filamentous bacteriophage containing a circular single-stranded DNA molecular, which is surrounded by approximately 2 800 copies of protein PVIII. In addition, there are five copies of each of proteins...M13 phage＇is a filamentous bacteriophage containing a circular single-stranded DNA molecular, which is surrounded by approximately 2 800 copies of protein PVIII. In addition, there are five copies of each of proteins PVII and PXI in one end, and five copies of each of proteins PVI and PUI on the other. These coat proteins have play an important role in the infection and assembly of M13 phage. With the development of phage display technology, these five coat proteins all can be used in phage display, which plays an increasingly important role in molecular detection and treatment.展开更多
Dear Editor,Atrial fibrillation(AF)is the most common sustained arrhythmia,frequently presenting with symptoms such as palpitations,fatigue,and shortness of breath(Lippi et al.,2021).AF is categorized into two distinc...Dear Editor,Atrial fibrillation(AF)is the most common sustained arrhythmia,frequently presenting with symptoms such as palpitations,fatigue,and shortness of breath(Lippi et al.,2021).AF is categorized into two distinct phenotypes:paroxysmal atrial fibrillation(PAF)and persistent atrial fibrillation(PsAF).展开更多
Sodium superionic conductor(NASICON)-type compounds have been regarded as promising cathodes for sodium-ion batteries(SIBs)due to their favorable ionic conductivity and robust structural stability.However,their high c...Sodium superionic conductor(NASICON)-type compounds have been regarded as promising cathodes for sodium-ion batteries(SIBs)due to their favorable ionic conductivity and robust structural stability.However,their high cost and relatively low energy density restrict their further practical application,which can be tailored by widening the operating voltages with earth-abundant elements such as Mn.Here,we propose a rational strategy of infusing Mn element in NASICON frameworks with sufficiently mobile sodium ions that enhances the redox voltage and ionic migration activity.The optimized structure of Na3.5Mn0.5V1.5(PO4)3/C is achieved and investigated systematically to be a durable cathode(76.6%capacity retention over 5,000 cycles at 20 C)for SIBs,which exhibits high reversible capacity(113.1 mAh·g^−1 at 0.5 C)with relatively low volume change(7.6%).Importantly,its high-areal-loading and temperature-resistant sodium ion storage properties are evaluated,and the full-cell configuration is demonstrated.This work indicates that this Na3.5Mn0.5V1.5(PO4)3/C composite could be a promising cathode candidate for SIBs.展开更多
Many studies have shown that spaceflight causes oxidative stress and induces brain disorder in astronauts,but the counter measurements are lacking.Increasing evidence demonstrated that hydrogen can act as a therapeuti...Many studies have shown that spaceflight causes oxidative stress and induces brain disorder in astronauts,but the counter measurements are lacking.Increasing evidence demonstrated that hydrogen can act as a therapeutic antioxidant.In this study,rats were treated with or without about 5%hydrogen under hindlimb unloading or normal conditions for 28 d.We assessed rat’s brain function by open-field test,step-down passive avoidance test,the neurotransmitter’s level detected by liquid chromatography with tandem mass spectrometry,and Nissl and hematoxylin-eosin staining analysis.We also assessed the oxidative damage by changes of malondialdehyde level,the ratio of reduced glutathione to oxidized glutathione,and superoxide dismutase and catalase activity.Glucose metabolism disorder was disclosed through glucose metabolomic analysis.The underlying mechanism of the effects of hydrogen was analyzed by mRNA sequencing and detecting mRNA and protein levels.Our data showed that hindlimb unloading caused oxidative damage and glucose metabolism disorder in brain tissues and decreased brain function in rats.Hydrogen inhalation reduced oxidative damage,ameliorated glucose metabolism disorder,and alleviated the dysfunction of rat brain function.Peroxisome-proliferator-activated receptor gamma coactivator 1αand brain-derived neurotrophic factor,the key regulators of glucose metabolism and brain function,were obviously affected.This research confirms the protective effect of hydrogen inhalation on declining brain function under hindlimb unloading conditions and discloses the underlying mechanism,which provides a potential strategy for astronauts’health.展开更多
文摘Objective：MicroRNAs （miRNAs） have potential as diagnostic biomarkers in cancer.Evaluation of the association between miRNA expression patterns and early detection of liver metastasis in colorectal cancer （CRC） has not been reported.Methods：We investigated the expression of metastasis-associated miRs-31,335,206,141,126,200b,200c,21,Let7a,Let7b and Let7c in localized,liver-metastatic and other organ-metastatic CRC （OM-CRC）.Expressions of target miRNAs in serum were evaluated in 116 consecutive localized CRC （L-CRC）,72 synchronous liver-metastatic CRC （SLM-CRC） and 36 other OM-CRC by quantitative real-time PCR.Results：Seven of 11 tested miRNAs could be detected from serum.Four miRNAs,miR-126,Let-7a,miR141 and miR-21 were identified as metastasis-associated miRNAs.Compared with L-CRC,significant upregulated expression was observed for miR-141 and miR-21 in SLM-CRC and OM-CRC,down-regulated expression was observed for miR-126 in SLM-CRC and OM-CRC,and up-regulated expression of Let-7a in OM-CRC.The receiver operating characteristic （ROC） curve showed serum miR-126 had a cut-off [log10 relative quantity （log10RQ）=--0.2005] with 77.78％ sensitivity and 68.97％ specificity with an area under curve （AUC） of 0.7564,miR-141 had a cut-off （1og10RQ=-0.2285） with 86.11％ sensitivity and 76.11％ specificity with an AUC of 0.8279,and miR-21 had a cut-off （log10RQ=-0.1310） with 73.61％ sensitivity and 66.38％ specificity with an AUC of 0.7479.Conclusions：We identified liver metastasis-associated miRNAs,suggesting serum miR-126,miR-141 and miR-21 may be novel biomarkers for clinical diagnosis of early stage liver-metastatic CRC.
基金Supported by 2011 Funding Project for Academic Human Resources Development(015000543111509)
文摘EBI2 is known as a kind of 7TM G protein-coupled receptors, and EBi2 gene is one of the most upregulated genes in Epstein-Barr virus （EBV） -infected lymphocytes. As a constitutively active receptor, EBI2 signals via Gai, and TM-II plays an important role in the signal transduct/on process. EBI2 is expressed mainly in mature, B cells and the expression level increases at the early stage of activation; subsequently, B cells are transferred into GC and the expression level is down-regulated. EBI2 can regulate independently and mediate synergistically the migration of B cell towards the extrafollicular areas and GC depending on chemo- kine receptors CXCR5 and CCR7 （EBII）. Differential expression of EBI2 receptor is conducive to accurate migration of B cells at different stages, thereby leading to appropriate irranunologic responses to T-dependent antigens. Recently, two research groups identified a specific oxysteml as the natural ligand for EBL2. Among all the oxysterols, 7α, 25-Dihydrexycholesteml （7a, 25-OHC） is the most effective ligand and activator, and EBI2-Oxysterol signal pathway plays an important role in adaptive immune responses. In addition, EBI2 is also closely related with specific kinds of autoimmune diseases, so it is considered as a negative regulator of the innate immune response in macrophages.
文摘M13 phage＇is a filamentous bacteriophage containing a circular single-stranded DNA molecular, which is surrounded by approximately 2 800 copies of protein PVIII. In addition, there are five copies of each of proteins PVII and PXI in one end, and five copies of each of proteins PVI and PUI on the other. These coat proteins have play an important role in the infection and assembly of M13 phage. With the development of phage display technology, these five coat proteins all can be used in phage display, which plays an increasingly important role in molecular detection and treatment.
基金supported by the National Natural Science Foundation of China(31500828).
文摘Dear Editor,Atrial fibrillation(AF)is the most common sustained arrhythmia,frequently presenting with symptoms such as palpitations,fatigue,and shortness of breath(Lippi et al.,2021).AF is categorized into two distinct phenotypes:paroxysmal atrial fibrillation(PAF)and persistent atrial fibrillation(PsAF).
基金This work was supported by the National Natural Science Foundation of China(Nos.51872334 and 51932011)the Innovation-Driven Project of Central South University(No.2020CX024).
文摘Sodium superionic conductor(NASICON)-type compounds have been regarded as promising cathodes for sodium-ion batteries(SIBs)due to their favorable ionic conductivity and robust structural stability.However,their high cost and relatively low energy density restrict their further practical application,which can be tailored by widening the operating voltages with earth-abundant elements such as Mn.Here,we propose a rational strategy of infusing Mn element in NASICON frameworks with sufficiently mobile sodium ions that enhances the redox voltage and ionic migration activity.The optimized structure of Na3.5Mn0.5V1.5(PO4)3/C is achieved and investigated systematically to be a durable cathode(76.6%capacity retention over 5,000 cycles at 20 C)for SIBs,which exhibits high reversible capacity(113.1 mAh·g^−1 at 0.5 C)with relatively low volume change(7.6%).Importantly,its high-areal-loading and temperature-resistant sodium ion storage properties are evaluated,and the full-cell configuration is demonstrated.This work indicates that this Na3.5Mn0.5V1.5(PO4)3/C composite could be a promising cathode candidate for SIBs.
基金supported by Advanced Space Medico-Engineering Research Project of China(no.2015SY54A0503)the National Natural Science Foundation of China(31630038,81830061,and 82192882).
文摘Many studies have shown that spaceflight causes oxidative stress and induces brain disorder in astronauts,but the counter measurements are lacking.Increasing evidence demonstrated that hydrogen can act as a therapeutic antioxidant.In this study,rats were treated with or without about 5%hydrogen under hindlimb unloading or normal conditions for 28 d.We assessed rat’s brain function by open-field test,step-down passive avoidance test,the neurotransmitter’s level detected by liquid chromatography with tandem mass spectrometry,and Nissl and hematoxylin-eosin staining analysis.We also assessed the oxidative damage by changes of malondialdehyde level,the ratio of reduced glutathione to oxidized glutathione,and superoxide dismutase and catalase activity.Glucose metabolism disorder was disclosed through glucose metabolomic analysis.The underlying mechanism of the effects of hydrogen was analyzed by mRNA sequencing and detecting mRNA and protein levels.Our data showed that hindlimb unloading caused oxidative damage and glucose metabolism disorder in brain tissues and decreased brain function in rats.Hydrogen inhalation reduced oxidative damage,ameliorated glucose metabolism disorder,and alleviated the dysfunction of rat brain function.Peroxisome-proliferator-activated receptor gamma coactivator 1αand brain-derived neurotrophic factor,the key regulators of glucose metabolism and brain function,were obviously affected.This research confirms the protective effect of hydrogen inhalation on declining brain function under hindlimb unloading conditions and discloses the underlying mechanism,which provides a potential strategy for astronauts’health.
