Breast cancer(BC)has the highest prevalence among cancers specific to women,and its incidence rates are increasing in many countries.Subtypes of BC,including HER2-positive or triple-negative BC,exhibit differing treat...Breast cancer(BC)has the highest prevalence among cancers specific to women,and its incidence rates are increasing in many countries.Subtypes of BC,including HER2-positive or triple-negative BC,exhibit differing treatment responses;consequently,demand for personalized therapy is increasing,and relevant precision medicine strategies are under development.Aerobic glycolysis in cancer cells can lead to excessive lactate production,which in turn promotes lactylation and influences tumor cell behavior.Epigenetic alterations and metabolic reprogramming are prominent characteristics of tumors.Because lactate and lactylation are important in cancer,further investigation of the mechanisms underlying lactate metabolism and lactylation,and the development of therapeutic strategies targeting these processes,are topics of increasing interest.This review describes current research on lactate metabolism and lactylation in BC,thus offering new perspectives for advancing treatment and management toward more precise and personalized approaches that will ultimately increase BC survival rates and patient quality of life.展开更多
The traditional Chinese medicine Buyang Huanwu Decoction has been shown to improve the neu- rological function of patients with stroke. However, the precise mechanisms underlying its effect remain poorly understood. I...The traditional Chinese medicine Buyang Huanwu Decoction has been shown to improve the neu- rological function of patients with stroke. However, the precise mechanisms underlying its effect remain poorly understood. In this study, we established a rat model of cerebral ischemia by middle cerebral artery occlusion and intragastrically administered 5 g/kg Buyang Huanwu Decoction, once per day, for 1, 7, 14 and 28 days after cerebral ischemia. Immunohistochemical staining revealed a number of cells positive for the neural stem cell marker nestin in the cerebral cortex, the subven- tricular zone and the ipsilateral hippocampal dentate gyrus in rat models of cerebral ischemia. Buyang Huanwu Decoction significantly increased the number of cells positive for 5-bromodeoxyuridine （BrdU）, a cell proliferation-related marker, microtubule-associated protein-2, a marker of neuronal differentiation, and growth-associated protein 43, a marker of synaptic plasticity in the ischemic rat cerebral regions. The number of positive cells peaked at 14 and 28 days after intragastric administration of Buyang Huanwu Decoction. These findings suggest that Buyang Huanwu Decoction can promote the proliferation and differentiation of neural stem cells and en- hance synaptic plasticity in ischemic rat brain tissue.展开更多
In Northeast China, permafrost advanced and retreated several times under the influences of fluctuating paleo-climatesand paleo-environments since the Late Pleistocene. During the last 60 years, many new data were obt...In Northeast China, permafrost advanced and retreated several times under the influences of fluctuating paleo-climatesand paleo-environments since the Late Pleistocene. During the last 60 years, many new data were obtained and studies wereconducted on the evolution of permafrost in Northeast China, but so far no systematic summary and review have been made.Based on sedimentary sequences, remains of past permafrost, paleo-flora and -fauna records, and dating data, permafrostevolution since the Late Pleistocene has been analyzed and reconstructed in this paper. Paleo-temperatures reconstructedfrom the remains of past permafrost and those from paleo-flora and -fauna are compared, and thus the southern limitof permafrost （SLP） in each climate period is inferred by the relationship of the permafrost distribution and the meanannual air/ground temperatures （MAAT/MAGT）. Thus, the evolutionary history of permafrost is here divided into fivestages： （1） the Late Pleistocene （Last Glaciation, or LG） （65 to 10–8.5 ka）, the Last Glaciation Maximum （LGM, 21–13 ka）in particular, the coldest period in the latest history with a cooling of about 6~10 °C, characterized by extensive occurrencesof glaciation, flourishing Mammathas-Coelodonta Faunal Complex （MCFC）, widespread aeolian deposits, and significantsea level lowering, and permafrost greatly expanded southwards almost to the coastal plains （37°N–41°N）; （2） the HoloceneMegathermal Period （HMP, 8.5–7.0 to 4.0–3.0 ka）, 3~5 °C warmer than today, permafrost retreated to about 52°N; （3） theLate Holocene Cold Period （Neoglaciation） （4.0–3.0 to 1.0–0.5 ka）, a cooling of 1~3 °C, some earlier thawed permafrost wasrefrozen or attached, and the SLP invaded southwards to 46°N; （4） the Little Ice Age （LIA, 500 to 100–150 a）, the latestcold period with significant permafrost expansion; and （5） climate warming since the last century, during which NortheastChina has undergone extensive permafrost degradation. The frequent and substantial expansions and retreats of permafrosthave greatly impacted cold-region environments in Northeast China. North of the SLP during the HMP, or in the presentcontinuous permafrost zone, the existing permafrost was largely formed during the LG and was later overlapped by thepermafrost formed in the Neoglaciation. To the south, it was formed in the Neoglaciation. However, many aspects ofpermafrost evolution still await further investigations, such as data integration, numerical reconstruction, and merging ofChinese permafrost history with those of bordering regions as well as collaboration with related disciplines. Of these, studies on the evolution and degradation of permafrost during the past 150 years and its hydrological, ecological, and environmentalimpacts should be prioritized.展开更多
Carbon nanotube-based(CNT-based) interfacial evaporation material is one of the most potential materials for solar desalination. Here, we studied the evaporation rate of the CNT-based membranes with different hydrophi...Carbon nanotube-based(CNT-based) interfacial evaporation material is one of the most potential materials for solar desalination. Here, we studied the evaporation rate of the CNT-based membranes with different hydrophilic and hydrophobic chemical modified surfaces using molecular dynamic simulations.We found that the hydrogen bonding density among water molecules at the interface is a key factor in enhancing the evaporation rate. For a hydrophilic CNT-based membrane, the strong interactions between the membrane outer surface and the water molecules can destroy the water-water hydrogen bonding interactions at the interface, resulting in the reduction of the hydrogen bonding density, leading to an enhancement effect in evaporation rate. We also found that there is an optimal thickness for evaporation membrane. These findings could provide some theoretical guidance for designing and exploring advanced CNT-based systems with more beneficial performance in water desalination.展开更多
The constitutive promoter of cauliflower mosaic virus 35S （CaMV 35S） is a polar unidirectional promoter and is widely used in plant genetic engineering. In the present study, the unidirectional CaMV 35S promoter has...The constitutive promoter of cauliflower mosaic virus 35S （CaMV 35S） is a polar unidirectional promoter and is widely used in plant genetic engineering. In the present study, the unidirectional CaMV 35S promoter has been modified to a bi-directional promoter by fusing its minimal promoter element to the 5＇ end of CaMV 35S promoter in the opposite orientation. To qualitatively and quantitatively estimate its bi-directional transcriptional function and activity, two visible reporter genes, gusA （13-glucuronidase, GUS） and gfp （green fluorescent protein, GFP）, were fused to the two ends of the promoter in bi-orientations ending with NOS terminator sequences, respectively. Stable expression of gusA and gfp genes in transgenic tobacco （Nicotiana tabacum L.） was visulized by histochemically staining for GUS and fluorescence microscopic observation under UV for GFP in transgenic plants. The expression of two reporter genes showed that the constructed bi-directional promoter did have the bi-directional transcriptional function in both expected orientations. The quantitative estimation of GUS and GFP were determined on a HITACHI F1000 Fluorescence Spectrophotometer with various wavelengths of excitation and emission. The GUS activity varied from g to 250 pmol 4-MU/min/mg protein and the GFP content varied from 0.9 to 1.8 μg/ mg protein in various lines of transgenic tobacco plants. Higher GUS activity generally coupled with lower GFP content, and vice versa.展开更多
Membrane trafficking is essential for plant growth and responses to external signals.The plant unique FYVE domain-containing protein FREE1 is a component of the ESCRT complex（endosomal sorting complex required for tr...Membrane trafficking is essential for plant growth and responses to external signals.The plant unique FYVE domain-containing protein FREE1 is a component of the ESCRT complex（endosomal sorting complex required for transport）.FREE1 plays multiple roles in regulating protein trafficking and organelle biogenesis including the formation of intraluminal vesicles of multivesicular body（MVB）,vacuolar protein transport and vacuole biogenesis,and autophagic degradation.FREE1 knockout plants show defective MVB formation,abnormal vacuolar transport,fragmented vacuoles,accumulated autophagosomes,and seedling lethality.To further uncover the underlying mechanisms of FREE1 function in plants,we performed a forward genetic screen for mutants that suppressed the seedling lethal phenotype of FREE1-RNAi transgenic plants.The obtained mutants are termed as suppressors of free1（sof）.To date,229 putative sof mutants have been identified.Barely detecting of FREE1 protein with M3 plants further identified 84 FREE1-related suppressors.Also145 mutants showing no reduction of FREE1 protein were termed as RNAi-related mutants.Through next-generation sequencing（NGS）of bulked DNA from F2 mapping population of two RNAi-related sof mutants,FREE1-RNAi T-DNA inserted on chromosome 1 was identified and the causal mutation of putative sof mutant is being identified similarly.These FREE1-and RNAi-related sof mutants will be useful tools and resources for illustrating the underlying mechanisms of FREE1 function in intracellular trafficking and organelle biogenesis,as well as for uncovering the new components involved in the regulation of silencing pathways in plants.展开更多
High-voltage-activated(HVA) Ca^(2+)channels are widely expressed in the nervous system. They play an important role in pain conduction by participating in various physiological processes such as synaptic transmission,...High-voltage-activated(HVA) Ca^(2+)channels are widely expressed in the nervous system. They play an important role in pain conduction by participating in various physiological processes such as synaptic transmission, changes in synaptic plasticity, and neuronal excitability. Available evidence suggests that the HVA channel is an important therapeutic target for pain management. In this review, we summarize the changes in different subtypes of HVA channel during pain and present the currently available evidence from the clinical application of HVA channel blockers. We also review novel drugs in various phases of development. Moreover, we discuss the future prospects of HVA channel blockers in order to promote ‘‘bench-to-bedside'' translation.展开更多
As an important component of tokamaks,the divertor is mainly responsible for extracting heat and helium ash,and the targets of the divertor need to withstand high heat flux of 10 MW m-2 for steady-state operation.In t...As an important component of tokamaks,the divertor is mainly responsible for extracting heat and helium ash,and the targets of the divertor need to withstand high heat flux of 10 MW m-2 for steady-state operation.In this study,we proposed a new strategy,using microchannel cooling technology to remove high heat load on the targets of the divertor.The results demonstrated that the microchannel-based W/Cu flat-type mock-up successfully withstood the thermal fatigue test of 1000 cycles at 10 MW m^(-2)with cooling water of 26 l min^(-1),30°C(inlet),0.8 MPa(inlet),15 s power on and 15 s dwell time;the maximum temperature on the heat-loaded surface(W surface)of the mock-up was 493°C,which is much lower than the recrystallization temperature of W(1200°C).Moreover,no occurrence of macrocrack and‘hot spot’at the W surface,as well as no detachment of W/Cu tiles were observed during the thermal fatigue testing.These results indicate that microchannel cooling technology is an efflcient method for removing the heat load of the divertor at a low flow rate.The present study offers a promising solution to replace the monoblock design for the EAST divertor.展开更多
Behavioral decision-making at urban intersections is one of the primary difficulties currently impeding the development of intelligent vehicle technology.The problem is that existing decision-making algorithms cannot ...Behavioral decision-making at urban intersections is one of the primary difficulties currently impeding the development of intelligent vehicle technology.The problem is that existing decision-making algorithms cannot effectively deal with complex random scenarios at urban intersections.To deal with this,a deep deterministic policy gradient(DDPG)decision-making algorithm(T-DDPG)based on a time-series Markov decision process(T-MDP)was developed,where the state was extended to collect observations from several consecutive frames.Experiments found that T-DDPG performed better in terms of convergence and generalizability in complex intersection scenarios than a traditional DDPG algorithm.Furthermore,model-agnostic meta-learning(MAML)was incorporated into the T-DDPG algorithm to improve the training method,leading to a decision algorithm(T-MAML-DDPG)based on a secondary gradient.Simulation experiments of intersection scenarios were carried out on the Gym-Carla platform to verify and compare the decision models.The results showed that T-MAML-DDPG was able to easily deal with the random states of complex intersection scenarios,which could improve traffic safety and efficiency.The above decision-making models based on meta-reinforcement learning are significant for enhancing the decision-making ability of intelligent vehicles at urban intersections.展开更多
Road lanes and markings are the bases for autonomous driving environment perception.In this paper,we propose an end-to-end multi-task network,Road All Information Extractor named RAIENet,which aims to extract the full...Road lanes and markings are the bases for autonomous driving environment perception.In this paper,we propose an end-to-end multi-task network,Road All Information Extractor named RAIENet,which aims to extract the full information of the road surface including road lanes,road markings and their correspondences.Based on the prior knowledge of pavement information,we explore and use the deep progressive relationship between lane segmentation and pavement mark-ing detection.Then,different attention mechanisms are adapted for different tasks.A lane detection accuracy of 0.807 F1-score and a ground marking accuracy of 0.971 mean average precision at intersection over union(IOU)threshold 0.5 were achieved on the newly labeled see more on road plus(CeyMo+)dataset.Of course,we also validated it on two well-known datasets Berkeley Deep-Drive 100K(BDD100K)and CULane.In addition,a post-processing method for generating bird’s eye view lane(BEVLane)using lidar point cloud information is proposed,which is used for the construction of high-definition maps and subsequent decision-making planning.The code and data are available at https://github.com/mayberpf/RAIEnet.展开更多
In this study,the resource distribution,morphological characteristics,artificial cultivation and market circulation of Paris Polyphylla var.yunnanensis and its polygerm varieties in Yunnan were investigated by referri...In this study,the resource distribution,morphological characteristics,artificial cultivation and market circulation of Paris Polyphylla var.yunnanensis and its polygerm varieties in Yunnan were investigated by referring to the research literature,field investigation and our planting experience.It is found that P.polyphylla var.yunnanensis is widely distributed in Yunnan Province,realizing large-scale artificial cultivation,while the wild resources of polygerm varieties are almost on the verge of extinction and are in urgent need of protection.展开更多
In order to respond to the national policy of energy saving and consumption reduction, the temperature drop formula of annular pipeline should be established to optimize the water mixing temperature and water mixing f...In order to respond to the national policy of energy saving and consumption reduction, the temperature drop formula of annular pipeline should be established to optimize the water mixing temperature and water mixing flow. The traditional temperature drop formula applies the high water cut actual situation to 13.7%. On the basis of the traditional Schulhof temperature drop formula, the influence of hydraulic friction on the oil flow temperature is calculated by the calculus idea. The heat transfer coefficient of the pipeline under the complex condition is obtained by the inverse algorithm, and the water mixing flow and water temperature are simulated with MATLAB. The function surface diagram of the degree and the back station temperature. The actual error can be reduced to 4.8%, and the return station temperature can be optimized by 7 degrees.展开更多
From December 2022 to January 2023,the SARS-CoV-2 Omicron BA.5/BF.7 variant significantly impacted China’s mainland.While most COVID-19 patients experienced mild symptoms and were treated as outpatients or at home,so...From December 2022 to January 2023,the SARS-CoV-2 Omicron BA.5/BF.7 variant significantly impacted China’s mainland.While most COVID-19 patients experienced mild symptoms and were treated as outpatients or at home,some cases progressed to severe illness,necessitating hospitalization or even resulting in death.To better understand this outbreak and forecast future waves as SARS-CoV-2 continues to evolve,it is crucial to assess the titer of neutralizing antibodies(Nab)for evaluating the establishment of an immune barrier.In this study,we investigated the dynamic evolution of humoral immunity following the breakthrough infection wave driven by the SARS-CoV-2 Omicron BA.5/BF.7 variant in southwest China.展开更多
The task of executing left turns at signal-free T-shaped intersections without protective signals poses a criticalchallenge in the realm of autonomous driving. Conventional rule-based approaches tend to be excessively...The task of executing left turns at signal-free T-shaped intersections without protective signals poses a criticalchallenge in the realm of autonomous driving. Conventional rule-based approaches tend to be excessivelycautious, rendering them inadequate for effectively managing driving tasks within unpredictable T-shapedintersection environments. In the case of complex traffic scenarios, a single model is less effective in convergenceand has a lower pass rate and poorer safety. Thus, this study introduces a multi-layer reinforcement learningmodel, employing D3QN (Dueling Double DQN) and TD3 (Twin Delayed Deep Deterministic policy gradientalgorithm) for advanced behavioral decision-making and vertical acceleration planning, respectively. In ourexperimental investigation, we designed four simulation scenarios based on the driving behavior of the Carlasimulator to replicate real-world driving conditions. Verification and test simulation outcomes substantiate that,in comparison to other single-trained reinforcement learning models, the multi-layer reinforcement learningmodel proposed in this study attains the highest success rate. Specifically, the pass rate in the verification scenario,consistent with the training conditions, achieves an impressive 99.5%. Furthermore, the pass rate in thecomprehensive test scenario reaches 89.6%. These experiments unequivocally demonstrate the considerableenhancement in T-shaped intersections pass rates achieved by the proposed method while ensuring both trafficefficiency and safety.展开更多
基金supported by grants from the National Natural Science Foundation of China(Grant No.82302626)Gusu Health Talent Project of Suzhou,China(Grant No.GSWS2022076)Suzhou Key Laboratory of Intelligent Critical Illness Biomarkers Translational Research Project(Grant No.SZS2024029)。
文摘Breast cancer(BC)has the highest prevalence among cancers specific to women,and its incidence rates are increasing in many countries.Subtypes of BC,including HER2-positive or triple-negative BC,exhibit differing treatment responses;consequently,demand for personalized therapy is increasing,and relevant precision medicine strategies are under development.Aerobic glycolysis in cancer cells can lead to excessive lactate production,which in turn promotes lactylation and influences tumor cell behavior.Epigenetic alterations and metabolic reprogramming are prominent characteristics of tumors.Because lactate and lactylation are important in cancer,further investigation of the mechanisms underlying lactate metabolism and lactylation,and the development of therapeutic strategies targeting these processes,are topics of increasing interest.This review describes current research on lactate metabolism and lactylation in BC,thus offering new perspectives for advancing treatment and management toward more precise and personalized approaches that will ultimately increase BC survival rates and patient quality of life.
基金supported by grants from the National Nature Science Foundation of China,No.30873355,81072939,81273989,81202694the Foundation of Educational Commission of Hunan Province in China,No.11C0954
文摘The traditional Chinese medicine Buyang Huanwu Decoction has been shown to improve the neu- rological function of patients with stroke. However, the precise mechanisms underlying its effect remain poorly understood. In this study, we established a rat model of cerebral ischemia by middle cerebral artery occlusion and intragastrically administered 5 g/kg Buyang Huanwu Decoction, once per day, for 1, 7, 14 and 28 days after cerebral ischemia. Immunohistochemical staining revealed a number of cells positive for the neural stem cell marker nestin in the cerebral cortex, the subven- tricular zone and the ipsilateral hippocampal dentate gyrus in rat models of cerebral ischemia. Buyang Huanwu Decoction significantly increased the number of cells positive for 5-bromodeoxyuridine （BrdU）, a cell proliferation-related marker, microtubule-associated protein-2, a marker of neuronal differentiation, and growth-associated protein 43, a marker of synaptic plasticity in the ischemic rat cerebral regions. The number of positive cells peaked at 14 and 28 days after intragastric administration of Buyang Huanwu Decoction. These findings suggest that Buyang Huanwu Decoction can promote the proliferation and differentiation of neural stem cells and en- hance synaptic plasticity in ischemic rat brain tissue.
基金supported by the Subproject No. XDA05120302 (Permafrost Extent in China during the Last Glaciation Maximum and Megathermal)Strategic Pilot Science and Technology Program of the Chinese Academy of Sciences (Identification of Carbon Budgets for Adaptation to Changing Climate and the Associated Issues) (Grant No. XDA05000000)the auspices of the International Permafrost Association (IPA) Action Group on "Last Permafrost Maximum and Minimum (LPMM) on the Eurasian Continent"
文摘In Northeast China, permafrost advanced and retreated several times under the influences of fluctuating paleo-climatesand paleo-environments since the Late Pleistocene. During the last 60 years, many new data were obtained and studies wereconducted on the evolution of permafrost in Northeast China, but so far no systematic summary and review have been made.Based on sedimentary sequences, remains of past permafrost, paleo-flora and -fauna records, and dating data, permafrostevolution since the Late Pleistocene has been analyzed and reconstructed in this paper. Paleo-temperatures reconstructedfrom the remains of past permafrost and those from paleo-flora and -fauna are compared, and thus the southern limitof permafrost （SLP） in each climate period is inferred by the relationship of the permafrost distribution and the meanannual air/ground temperatures （MAAT/MAGT）. Thus, the evolutionary history of permafrost is here divided into fivestages： （1） the Late Pleistocene （Last Glaciation, or LG） （65 to 10–8.5 ka）, the Last Glaciation Maximum （LGM, 21–13 ka）in particular, the coldest period in the latest history with a cooling of about 6~10 °C, characterized by extensive occurrencesof glaciation, flourishing Mammathas-Coelodonta Faunal Complex （MCFC）, widespread aeolian deposits, and significantsea level lowering, and permafrost greatly expanded southwards almost to the coastal plains （37°N–41°N）; （2） the HoloceneMegathermal Period （HMP, 8.5–7.0 to 4.0–3.0 ka）, 3~5 °C warmer than today, permafrost retreated to about 52°N; （3） theLate Holocene Cold Period （Neoglaciation） （4.0–3.0 to 1.0–0.5 ka）, a cooling of 1~3 °C, some earlier thawed permafrost wasrefrozen or attached, and the SLP invaded southwards to 46°N; （4） the Little Ice Age （LIA, 500 to 100–150 a）, the latestcold period with significant permafrost expansion; and （5） climate warming since the last century, during which NortheastChina has undergone extensive permafrost degradation. The frequent and substantial expansions and retreats of permafrosthave greatly impacted cold-region environments in Northeast China. North of the SLP during the HMP, or in the presentcontinuous permafrost zone, the existing permafrost was largely formed during the LG and was later overlapped by thepermafrost formed in the Neoglaciation. To the south, it was formed in the Neoglaciation. However, many aspects ofpermafrost evolution still await further investigations, such as data integration, numerical reconstruction, and merging ofChinese permafrost history with those of bordering regions as well as collaboration with related disciplines. Of these, studies on the evolution and degradation of permafrost during the past 150 years and its hydrological, ecological, and environmentalimpacts should be prioritized.
基金supported by the National Key R&D Program of China (No. 2018YFA0209500)the National Natural Science Foundation of China (Nos. 52025132, 21975209, 22005255)the Key Laboratory of Biomedical Effects of Nanomaterials and Nanosafety,Chinese Academy of Sciences (No. NSKF202008)。
文摘Carbon nanotube-based(CNT-based) interfacial evaporation material is one of the most potential materials for solar desalination. Here, we studied the evaporation rate of the CNT-based membranes with different hydrophilic and hydrophobic chemical modified surfaces using molecular dynamic simulations.We found that the hydrogen bonding density among water molecules at the interface is a key factor in enhancing the evaporation rate. For a hydrophilic CNT-based membrane, the strong interactions between the membrane outer surface and the water molecules can destroy the water-water hydrogen bonding interactions at the interface, resulting in the reduction of the hydrogen bonding density, leading to an enhancement effect in evaporation rate. We also found that there is an optimal thickness for evaporation membrane. These findings could provide some theoretical guidance for designing and exploring advanced CNT-based systems with more beneficial performance in water desalination.
文摘The constitutive promoter of cauliflower mosaic virus 35S （CaMV 35S） is a polar unidirectional promoter and is widely used in plant genetic engineering. In the present study, the unidirectional CaMV 35S promoter has been modified to a bi-directional promoter by fusing its minimal promoter element to the 5＇ end of CaMV 35S promoter in the opposite orientation. To qualitatively and quantitatively estimate its bi-directional transcriptional function and activity, two visible reporter genes, gusA （13-glucuronidase, GUS） and gfp （green fluorescent protein, GFP）, were fused to the two ends of the promoter in bi-orientations ending with NOS terminator sequences, respectively. Stable expression of gusA and gfp genes in transgenic tobacco （Nicotiana tabacum L.） was visulized by histochemically staining for GUS and fluorescence microscopic observation under UV for GFP in transgenic plants. The expression of two reporter genes showed that the constructed bi-directional promoter did have the bi-directional transcriptional function in both expected orientations. The quantitative estimation of GUS and GFP were determined on a HITACHI F1000 Fluorescence Spectrophotometer with various wavelengths of excitation and emission. The GUS activity varied from g to 250 pmol 4-MU/min/mg protein and the GFP content varied from 0.9 to 1.8 μg/ mg protein in various lines of transgenic tobacco plants. Higher GUS activity generally coupled with lower GFP content, and vice versa.
基金supported by grants from the NIH GM114660 to Y.Zhaothe Research Grants Council of Hong Kong (CUHK466011,465112,466613,CUHK2/CRF/11G,C401114R and Ao E/M-05/12)+2 种基金NSFC/RGC (N_CUHK406/12)NSFC (31270226 and 31470294)Shenzhen Peacock Project (KQTD201101) to L.Jiang
文摘Membrane trafficking is essential for plant growth and responses to external signals.The plant unique FYVE domain-containing protein FREE1 is a component of the ESCRT complex（endosomal sorting complex required for transport）.FREE1 plays multiple roles in regulating protein trafficking and organelle biogenesis including the formation of intraluminal vesicles of multivesicular body（MVB）,vacuolar protein transport and vacuole biogenesis,and autophagic degradation.FREE1 knockout plants show defective MVB formation,abnormal vacuolar transport,fragmented vacuoles,accumulated autophagosomes,and seedling lethality.To further uncover the underlying mechanisms of FREE1 function in plants,we performed a forward genetic screen for mutants that suppressed the seedling lethal phenotype of FREE1-RNAi transgenic plants.The obtained mutants are termed as suppressors of free1（sof）.To date,229 putative sof mutants have been identified.Barely detecting of FREE1 protein with M3 plants further identified 84 FREE1-related suppressors.Also145 mutants showing no reduction of FREE1 protein were termed as RNAi-related mutants.Through next-generation sequencing（NGS）of bulked DNA from F2 mapping population of two RNAi-related sof mutants,FREE1-RNAi T-DNA inserted on chromosome 1 was identified and the causal mutation of putative sof mutant is being identified similarly.These FREE1-and RNAi-related sof mutants will be useful tools and resources for illustrating the underlying mechanisms of FREE1 function in intracellular trafficking and organelle biogenesis,as well as for uncovering the new components involved in the regulation of silencing pathways in plants.
基金supported by the National Natural Science Foundation of China (81701092)Shanghai Municipal Education Commission-Gaofeng Clinical Medicine Support (20171916)Shanghai Municipal Commission of Health and Family Planning Funding for Key Developing Disciplines (2015ZB0101)
文摘High-voltage-activated(HVA) Ca^(2+)channels are widely expressed in the nervous system. They play an important role in pain conduction by participating in various physiological processes such as synaptic transmission, changes in synaptic plasticity, and neuronal excitability. Available evidence suggests that the HVA channel is an important therapeutic target for pain management. In this review, we summarize the changes in different subtypes of HVA channel during pain and present the currently available evidence from the clinical application of HVA channel blockers. We also review novel drugs in various phases of development. Moreover, we discuss the future prospects of HVA channel blockers in order to promote ‘‘bench-to-bedside'' translation.
基金financial support from the National MCF Energy R&D Program(No.2018YFE0312300)National Natural Science Foundation of China(No.51706100)+1 种基金the Natural Science Foundation of Jiangsu Province(No.BK20180477)Fundamental Research Funds for the Central Universities(No.30918011205)。
文摘As an important component of tokamaks,the divertor is mainly responsible for extracting heat and helium ash,and the targets of the divertor need to withstand high heat flux of 10 MW m-2 for steady-state operation.In this study,we proposed a new strategy,using microchannel cooling technology to remove high heat load on the targets of the divertor.The results demonstrated that the microchannel-based W/Cu flat-type mock-up successfully withstood the thermal fatigue test of 1000 cycles at 10 MW m^(-2)with cooling water of 26 l min^(-1),30°C(inlet),0.8 MPa(inlet),15 s power on and 15 s dwell time;the maximum temperature on the heat-loaded surface(W surface)of the mock-up was 493°C,which is much lower than the recrystallization temperature of W(1200°C).Moreover,no occurrence of macrocrack and‘hot spot’at the W surface,as well as no detachment of W/Cu tiles were observed during the thermal fatigue testing.These results indicate that microchannel cooling technology is an efflcient method for removing the heat load of the divertor at a low flow rate.The present study offers a promising solution to replace the monoblock design for the EAST divertor.
基金supported in part by the Beijing Municipal Science and Technology Project(No.Z191100007419010)Automobile Industry Joint Fund(No.U1764261)of the National Natural Science Foundation of China+1 种基金Shandong Key R&D Program(No.2020CXGC010118)Key Laboratory for New Technology Application of Road Conveyance of Jiangsu Province(No.BM20082061706)。
文摘Behavioral decision-making at urban intersections is one of the primary difficulties currently impeding the development of intelligent vehicle technology.The problem is that existing decision-making algorithms cannot effectively deal with complex random scenarios at urban intersections.To deal with this,a deep deterministic policy gradient(DDPG)decision-making algorithm(T-DDPG)based on a time-series Markov decision process(T-MDP)was developed,where the state was extended to collect observations from several consecutive frames.Experiments found that T-DDPG performed better in terms of convergence and generalizability in complex intersection scenarios than a traditional DDPG algorithm.Furthermore,model-agnostic meta-learning(MAML)was incorporated into the T-DDPG algorithm to improve the training method,leading to a decision algorithm(T-MAML-DDPG)based on a secondary gradient.Simulation experiments of intersection scenarios were carried out on the Gym-Carla platform to verify and compare the decision models.The results showed that T-MAML-DDPG was able to easily deal with the random states of complex intersection scenarios,which could improve traffic safety and efficiency.The above decision-making models based on meta-reinforcement learning are significant for enhancing the decision-making ability of intelligent vehicles at urban intersections.
基金supported by the Key R&D Program of Shandong Province,China(No.2020CXGC010118)Advanced Technology Research Institute,Beijing Institute of Technology(BITAI).
文摘Road lanes and markings are the bases for autonomous driving environment perception.In this paper,we propose an end-to-end multi-task network,Road All Information Extractor named RAIENet,which aims to extract the full information of the road surface including road lanes,road markings and their correspondences.Based on the prior knowledge of pavement information,we explore and use the deep progressive relationship between lane segmentation and pavement mark-ing detection.Then,different attention mechanisms are adapted for different tasks.A lane detection accuracy of 0.807 F1-score and a ground marking accuracy of 0.971 mean average precision at intersection over union(IOU)threshold 0.5 were achieved on the newly labeled see more on road plus(CeyMo+)dataset.Of course,we also validated it on two well-known datasets Berkeley Deep-Drive 100K(BDD100K)and CULane.In addition,a post-processing method for generating bird’s eye view lane(BEVLane)using lidar point cloud information is proposed,which is used for the construction of high-definition maps and subsequent decision-making planning.The code and data are available at https://github.com/mayberpf/RAIEnet.
基金Sichuan Provincial Department of Science and Technology(2017SZYZF0004).
文摘In this study,the resource distribution,morphological characteristics,artificial cultivation and market circulation of Paris Polyphylla var.yunnanensis and its polygerm varieties in Yunnan were investigated by referring to the research literature,field investigation and our planting experience.It is found that P.polyphylla var.yunnanensis is widely distributed in Yunnan Province,realizing large-scale artificial cultivation,while the wild resources of polygerm varieties are almost on the verge of extinction and are in urgent need of protection.
文摘In order to respond to the national policy of energy saving and consumption reduction, the temperature drop formula of annular pipeline should be established to optimize the water mixing temperature and water mixing flow. The traditional temperature drop formula applies the high water cut actual situation to 13.7%. On the basis of the traditional Schulhof temperature drop formula, the influence of hydraulic friction on the oil flow temperature is calculated by the calculus idea. The heat transfer coefficient of the pipeline under the complex condition is obtained by the inverse algorithm, and the water mixing flow and water temperature are simulated with MATLAB. The function surface diagram of the degree and the back station temperature. The actual error can be reduced to 4.8%, and the return station temperature can be optimized by 7 degrees.
基金supported by National Science and Technology Major Project of the Ministry of Science and Technology of China(2024ZD0523902)the National Natural Science Foundation of China(81790643,82121003,82103986)+5 种基金the CAMS Innovation Fund for Medical Sciences(2019-I2M-5-032)the Sichuan Science and Technology Program(2025ZNSFSC0793,2022YFS0002)Sichuan Medical Association(Q2024079)the Health Department of Sichuan Province(2022-214,21PJ081,S21010)Chengdu Science and Technology Bureau(2022-YF05-01644-SN)the Sichuan Provincial People’s Hospital(2022QN41).
文摘From December 2022 to January 2023,the SARS-CoV-2 Omicron BA.5/BF.7 variant significantly impacted China’s mainland.While most COVID-19 patients experienced mild symptoms and were treated as outpatients or at home,some cases progressed to severe illness,necessitating hospitalization or even resulting in death.To better understand this outbreak and forecast future waves as SARS-CoV-2 continues to evolve,it is crucial to assess the titer of neutralizing antibodies(Nab)for evaluating the establishment of an immune barrier.In this study,we investigated the dynamic evolution of humoral immunity following the breakthrough infection wave driven by the SARS-CoV-2 Omicron BA.5/BF.7 variant in southwest China.
基金support by the Key R&D Program of Shandong Province,China(No.2023CXPT032)Shandong Provincial Natural Science Foundation(No.ZR2023MF0766).
文摘The task of executing left turns at signal-free T-shaped intersections without protective signals poses a criticalchallenge in the realm of autonomous driving. Conventional rule-based approaches tend to be excessivelycautious, rendering them inadequate for effectively managing driving tasks within unpredictable T-shapedintersection environments. In the case of complex traffic scenarios, a single model is less effective in convergenceand has a lower pass rate and poorer safety. Thus, this study introduces a multi-layer reinforcement learningmodel, employing D3QN (Dueling Double DQN) and TD3 (Twin Delayed Deep Deterministic policy gradientalgorithm) for advanced behavioral decision-making and vertical acceleration planning, respectively. In ourexperimental investigation, we designed four simulation scenarios based on the driving behavior of the Carlasimulator to replicate real-world driving conditions. Verification and test simulation outcomes substantiate that,in comparison to other single-trained reinforcement learning models, the multi-layer reinforcement learningmodel proposed in this study attains the highest success rate. Specifically, the pass rate in the verification scenario,consistent with the training conditions, achieves an impressive 99.5%. Furthermore, the pass rate in thecomprehensive test scenario reaches 89.6%. These experiments unequivocally demonstrate the considerableenhancement in T-shaped intersections pass rates achieved by the proposed method while ensuring both trafficefficiency and safety.
