Acetylcholinesterase (AChE) plays a key role in terminating neurotransmission at cholinergic synapses. AChE is also found in tissues devoid of cholinergic responses, indicating its potential function beyond neurotrans...Acetylcholinesterase (AChE) plays a key role in terminating neurotransmission at cholinergic synapses. AChE is also found in tissues devoid of cholinergic responses, indicating its potential function beyond neurotransmission. It has been suggested that AChE may participate in development, differentiation, and pathogenic processes such as Alzheimer's disease and tumorigenesis. We examined AChE expression in human lung fibroblast cell line (HLF) upon induction of apoptosis by UV or G418. AChE is induced in all apoptotic cells examined as determined by cytochemical staining, immunological analysis,展开更多
Activin A is a kind of pre-inflammatory factor that belongs to the transforming growth factor-β(TGF-β) superfamily.To investigate the effect and mechanism of activin A on the activities of mouse macrophages,the secr...Activin A is a kind of pre-inflammatory factor that belongs to the transforming growth factor-β(TGF-β) superfamily.To investigate the effect and mechanism of activin A on the activities of mouse macrophages,the secretion of NO in the supernatant of cultured mouse peritoneal macrophages was examined by NO assay kit,and the expression of iNOS,ActRIIA and ARIP2 mRNA in mouse peritoneal macrophages was analyzed by RT-PCR. The results showed that activin A stimulated the secretion of NO and the expression of iNOS mRNA in non-activated mouse macrophages in a time-and dose-dependent manner.In contrast,activin A in the same concentration inhibited the secretion of NO in LPS-activated mouse macrophages in a dose-dependent manner. ActRIIA was highly expressed on macrophages,and activin A upregulated the ActRIIA mRNA expression in macrophages.Anti-ActRIIA antibody can block the secretion of NO from the macrophages stimulated by activin A. Furthermore,RT-PCR analysis revealed that activin A enhanced the ARIP2 mRNA expression in macrophages. These results indicated that Activin A may be a weak activator compared with LPS to mouse macrophages,and activin A may modulate the secretion of NO through ActRIIA-ARIP2 signal pathway in mouse macrophages. Cellular & Molecular Immunology.2005;2(1):63-67.展开更多
文摘Acetylcholinesterase (AChE) plays a key role in terminating neurotransmission at cholinergic synapses. AChE is also found in tissues devoid of cholinergic responses, indicating its potential function beyond neurotransmission. It has been suggested that AChE may participate in development, differentiation, and pathogenic processes such as Alzheimer's disease and tumorigenesis. We examined AChE expression in human lung fibroblast cell line (HLF) upon induction of apoptosis by UV or G418. AChE is induced in all apoptotic cells examined as determined by cytochemical staining, immunological analysis,
基金supported by the National Natural Science Foundation of China(No.30170478,30400400)International Cooperation Projects of Jilin Province of China(No.20040707-6).
文摘Activin A is a kind of pre-inflammatory factor that belongs to the transforming growth factor-β(TGF-β) superfamily.To investigate the effect and mechanism of activin A on the activities of mouse macrophages,the secretion of NO in the supernatant of cultured mouse peritoneal macrophages was examined by NO assay kit,and the expression of iNOS,ActRIIA and ARIP2 mRNA in mouse peritoneal macrophages was analyzed by RT-PCR. The results showed that activin A stimulated the secretion of NO and the expression of iNOS mRNA in non-activated mouse macrophages in a time-and dose-dependent manner.In contrast,activin A in the same concentration inhibited the secretion of NO in LPS-activated mouse macrophages in a dose-dependent manner. ActRIIA was highly expressed on macrophages,and activin A upregulated the ActRIIA mRNA expression in macrophages.Anti-ActRIIA antibody can block the secretion of NO from the macrophages stimulated by activin A. Furthermore,RT-PCR analysis revealed that activin A enhanced the ARIP2 mRNA expression in macrophages. These results indicated that Activin A may be a weak activator compared with LPS to mouse macrophages,and activin A may modulate the secretion of NO through ActRIIA-ARIP2 signal pathway in mouse macrophages. Cellular & Molecular Immunology.2005;2(1):63-67.
