Here,we investigated the antitumor effect of adenovirus-mediated gene transfer of LIGHT,the tumor-necrosis factor(TNF)superfamily member also known as TNFSF14,in the murine A20 B-cell lymphoma.LIGHT gene modification ...Here,we investigated the antitumor effect of adenovirus-mediated gene transfer of LIGHT,the tumor-necrosis factor(TNF)superfamily member also known as TNFSF14,in the murine A20 B-cell lymphoma.LIGHT gene modification resulted in upregulated expression of Fas and the accessory molecule-intercellular adhesion molecule-1(ICAM-1)on A20 cells and led to enhanced A20 cell apoptosis.LIGHT-modified A20 cells effectively stimulated the proliferation of T lymphocytes and interferon(IFN)-c production in vitro.Immunization of BALB/c mice with a LIGHT-modified A20 cell vaccine efficiently elicited protective immunity against challenge with the parental tumor cell line.Adenovirus-mediated gene transfer of LIGHT by intratumoral injection exerted a very potent antitumor effect against pre-existing A20 cell lymphoma in BALB/c mice.This adenovirus-mediated LIGHT therapy induced substantial splenic natural killer(NK)and cytotoxic T lymphocyte(CTL)activity,enhanced tumor infiltration by inflammatory cells and increased chemokine expression of CC chemokine ligand 21(CCL21),IFN-inducible protein-10(IP-10)and monokine induced by IFN-c(Mig)from tumor tissues.Thus,adenovirus-mediated LIGHT therapy might have potential utility for the prevention and treatment of B-cell lymphoma.展开更多
In this article,published online on 26 April 2010,there was an unintended error in Fig.2a during proof revision process when submitted higher resolution figures as the typeset company needs,leading to that ICAM-1 FACS...In this article,published online on 26 April 2010,there was an unintended error in Fig.2a during proof revision process when submitted higher resolution figures as the typeset company needs,leading to that ICAM-1 FACS staining pictures are the same for A20/LacZ group and A20/LIGHT group.The picture of ICAM-1 FACS staining for A20/LacZ group has now been replaced with the correct picture which was shown in the proof.The corrected Fig.2a is shown here.We are sorry for the inconvenience caused by this error.展开更多
基金supported by grants from the National Natural Science Foundation of China(30328011 and 30872377)the National Basic Research Program of China(2004CB518802)the Science and Technology Department of Zhejiang Province(2008C23044).
文摘Here,we investigated the antitumor effect of adenovirus-mediated gene transfer of LIGHT,the tumor-necrosis factor(TNF)superfamily member also known as TNFSF14,in the murine A20 B-cell lymphoma.LIGHT gene modification resulted in upregulated expression of Fas and the accessory molecule-intercellular adhesion molecule-1(ICAM-1)on A20 cells and led to enhanced A20 cell apoptosis.LIGHT-modified A20 cells effectively stimulated the proliferation of T lymphocytes and interferon(IFN)-c production in vitro.Immunization of BALB/c mice with a LIGHT-modified A20 cell vaccine efficiently elicited protective immunity against challenge with the parental tumor cell line.Adenovirus-mediated gene transfer of LIGHT by intratumoral injection exerted a very potent antitumor effect against pre-existing A20 cell lymphoma in BALB/c mice.This adenovirus-mediated LIGHT therapy induced substantial splenic natural killer(NK)and cytotoxic T lymphocyte(CTL)activity,enhanced tumor infiltration by inflammatory cells and increased chemokine expression of CC chemokine ligand 21(CCL21),IFN-inducible protein-10(IP-10)and monokine induced by IFN-c(Mig)from tumor tissues.Thus,adenovirus-mediated LIGHT therapy might have potential utility for the prevention and treatment of B-cell lymphoma.
文摘In this article,published online on 26 April 2010,there was an unintended error in Fig.2a during proof revision process when submitted higher resolution figures as the typeset company needs,leading to that ICAM-1 FACS staining pictures are the same for A20/LacZ group and A20/LIGHT group.The picture of ICAM-1 FACS staining for A20/LacZ group has now been replaced with the correct picture which was shown in the proof.The corrected Fig.2a is shown here.We are sorry for the inconvenience caused by this error.
