Despite the promise of nanomedicines leveraging the enhanced permeability and retention(EPR)effect for tumor targeting,their clinical translation remains hindered due to physiological barriers,rapid clearance,and poor...Despite the promise of nanomedicines leveraging the enhanced permeability and retention(EPR)effect for tumor targeting,their clinical translation remains hindered due to physiological barriers,rapid clearance,and poor intratumoral diffusion.In situ self-assembly of nanomedicines within tumors offers a promising strategy to enhance tumor accumulation.However,significant challenges persist.Herein,our findings reveal that tumor-infiltrating erythrocytes demonstrate the capacity to endogenously synthesize self-assembled nanomedicine with clinically approved small-molecule drug.Attenuated Salmonella Typhimurium VNP20009(VNP)can induce tumor-specific erythrocyte infiltration.Erythrocytes infiltrating the tumor site are phagocytosed by tumor cells and degraded into ferrous ions through the action of heme oxygenase.In the presence of zoledronic acid,ferrous irons interact with the zoledronic acid,resulting in the intracellular self-assembly of nanoparticles within tumor cells.These nanoparticles exhibit peroxidase-mimetic activity,and continuously catalyze the generation of reactive oxygen species(ROS)within the tumor,ultimately inducing tumor cell apoptosis.Therefore,this bioengineered therapeutic strategy that harnesses erythrocyte phagocytosis to drive tumor-specific intracellular self-assembly of peroxidase-mimetic nanoparticles may open up new nanotechnology for drug delivery and improve antitumor therapy.展开更多
基金supported by a grant from the National Key R&D Program of China(No.2022YFC3401600)the National Natural Science Foundation of China(No.32171372)+2 种基金Central Guidance for Local Science and Technology Development Funds Project(No.XZ202202YD0029C)Special Funds of Life and Health Technology of Naning(No.202110020)the National Natural Science Foundation of China(No.82241054)。
文摘Despite the promise of nanomedicines leveraging the enhanced permeability and retention(EPR)effect for tumor targeting,their clinical translation remains hindered due to physiological barriers,rapid clearance,and poor intratumoral diffusion.In situ self-assembly of nanomedicines within tumors offers a promising strategy to enhance tumor accumulation.However,significant challenges persist.Herein,our findings reveal that tumor-infiltrating erythrocytes demonstrate the capacity to endogenously synthesize self-assembled nanomedicine with clinically approved small-molecule drug.Attenuated Salmonella Typhimurium VNP20009(VNP)can induce tumor-specific erythrocyte infiltration.Erythrocytes infiltrating the tumor site are phagocytosed by tumor cells and degraded into ferrous ions through the action of heme oxygenase.In the presence of zoledronic acid,ferrous irons interact with the zoledronic acid,resulting in the intracellular self-assembly of nanoparticles within tumor cells.These nanoparticles exhibit peroxidase-mimetic activity,and continuously catalyze the generation of reactive oxygen species(ROS)within the tumor,ultimately inducing tumor cell apoptosis.Therefore,this bioengineered therapeutic strategy that harnesses erythrocyte phagocytosis to drive tumor-specific intracellular self-assembly of peroxidase-mimetic nanoparticles may open up new nanotechnology for drug delivery and improve antitumor therapy.
