AIM： To investigate genetic instability of gene BRCA1 at locus D17S855, and their relationship with clinicopathological characteristics of gastric cancer in Chinese population. METHODS： Microsatellite instability （...AIM： To investigate genetic instability of gene BRCA1 at locus D17S855, and their relationship with clinicopathological characteristics of gastric cancer in Chinese population. METHODS： Microsatellite instability （MSI） and loss of heterozygosity （LOH） of gene BRCA1 at locus D17S855 were compared between 37 samples of gastric cancer and corresponding non-cancerous gastric tissue. RESULTS： MSI at locus D17S855 was positive in 7 of 37 samples of gastric cancer （18.95%）. MSI had a close relationship with TNM staging but no relation with lymph node metastasis, histological type or tumor differentiation. MSI positive frequency in TNM Ⅰ ＋ Ⅱ （31.58%, 6/19） was much higher than that in TNM Ⅲ＋ Ⅳ （5.56%, 1/18）, （P 〈 0.05）. LOH positive rate was 18.92% （7/37）. LOH had no relationship to histological type, tumor differentiation or lymph node metastasis, but LOH positive rate in TNM Ⅲ＋ Ⅳ was 33.33% （6/18）, much higher than that in TNM Ⅰ ＋ Ⅱ （ 5.26%, 1/19）, （P 〈 0.05）. BRCA1 protein was expressed in 14 of 37 samples of gastric cancer. The positive rates of BRCA1 protein in TNM Ⅰ ＋ Ⅱ and TNM Ⅲ＋ Ⅳ were 57.89% and 16.67%, respectively, （P 〈 0.05）. The positive rate of BRCA1 protein was 77.78% in high differentiation samples, 30.77% in middle differentiation and 12.50% in lower differentiation samples, （P 〈 0.05）. CONCLUSION： MSI of BRCA1 gene could be used as a molecular marker in early phases of sporadic gastric cancer in Chinese population. LOH occurs at later period of gastric cancer, therefore, it could be used as prognostic factor.展开更多
BACKGROUND Pulmonary alveolar proteinosis(PAP)is a rare lung disease characterized by the accumulation of phospholipoproteinaceous material in the alveoli.Cases of PAP complicated with tuberculosis are much more compl...BACKGROUND Pulmonary alveolar proteinosis(PAP)is a rare lung disease characterized by the accumulation of phospholipoproteinaceous material in the alveoli.Cases of PAP complicated with tuberculosis are much more complex and have rarely been well recorded.CASE SUMMARY We describe a 21-year-old Han Chinese patient with suspicious lung infection associated with mild restrictive ventilatory dysfunction and diffusion reduction.High resolution computed tomography revealed a“crazy-paving”appearance and multiple pulmonary miliary nodules around the bronchi.Bronchoalveolar lavage demonstrated a small amount of periodic acid-Schiff positive proteinaceous materials.A serological test for the presence of a Mycobacterium tuberculosis antibody and an interferon-gamma release assay were both positive.The patient received a standard course of first-line anti-tuberculosis treatment after diagnostic bronchoalveolar lavage.To date,clinical remission has been achieved and maintained for five years.CONCLUSION In summary,the diagnosis of PAP complicated with tuberculosis was supported by a combination of clinical manifestations,imaging,pulmonary function,laboratory examinations,bronchoalveolar lavage,etc.This case highlighted that diagnostic bronchoalveolar lavage in combination with anti-tuberculosis treatment is a safe and effective option for mild PAP patients with tuberculosis.展开更多
Spontaneous rupture of the iliac vein is rare clinical emergency. Sudden onset, hypertension, and abdominal distention with a nonpulsatile mass in the iliac fossa are the chief symptoms. We reported another case of sp...Spontaneous rupture of the iliac vein is rare clinical emergency. Sudden onset, hypertension, and abdominal distention with a nonpulsatile mass in the iliac fossa are the chief symptoms. We reported another case of spontaneous rupture of the left external iliac vein diagnosed intra-operatively and successfully treated with direct suturing. And related literatures were reviewed. Possible etiology and optimal treatment were discussed.展开更多
Length-biased data arise in many important fields, including epidemiological cohort studies, cancer screening trials and labor economics. Analysis of such data has attracted much attention in the literature. In this p...Length-biased data arise in many important fields, including epidemiological cohort studies, cancer screening trials and labor economics. Analysis of such data has attracted much attention in the literature. In this paper we propose a quantile regression approach for analyzing right-censored and length-biased data. We derive an inverse probability weighted estimating equation corresponding to the quantile regression to correct the bias due to length-bias sampling and informative censoring. This method can easily handle informative censoring induced by length-biased sampling. This is an appealing feature of our proposed method since it is generally difficult to obtain unbiased estimates of risk factors in the presence of length-bias and informative censoring. We establish the consistency and asymptotic distribution of the proposed estimator using empirical process techniques. A resampling method is adopted to estimate the variance of the estimator. We conduct simulation studies to evaluate its finite sample performance and use a real data set to illustrate the application of the proposed method.展开更多
Wernicke encephalopathy(WE)is an acute or subacute neurological syndrome caused by thiamine(Vitamin B 1)deficiency and is usually underestimated in clinical practice.WE is suspected in merely about 6%of non-alcoholic ...Wernicke encephalopathy(WE)is an acute or subacute neurological syndrome caused by thiamine(Vitamin B 1)deficiency and is usually underestimated in clinical practice.WE is suspected in merely about 6%of non-alcoholic patients and one-third of alcoholic patients,Non-alcoholic causes include gastrointestinal surgery and disease,malnutrition,cancer and chemotherapeutic treatments,and long-term parenteral nutrition.However,few cases were reported about WE in patients with tuberculosis.Early diagnosis and medication are vital for the prognosis of this disease.展开更多
文摘AIM： To investigate genetic instability of gene BRCA1 at locus D17S855, and their relationship with clinicopathological characteristics of gastric cancer in Chinese population. METHODS： Microsatellite instability （MSI） and loss of heterozygosity （LOH） of gene BRCA1 at locus D17S855 were compared between 37 samples of gastric cancer and corresponding non-cancerous gastric tissue. RESULTS： MSI at locus D17S855 was positive in 7 of 37 samples of gastric cancer （18.95%）. MSI had a close relationship with TNM staging but no relation with lymph node metastasis, histological type or tumor differentiation. MSI positive frequency in TNM Ⅰ ＋ Ⅱ （31.58%, 6/19） was much higher than that in TNM Ⅲ＋ Ⅳ （5.56%, 1/18）, （P 〈 0.05）. LOH positive rate was 18.92% （7/37）. LOH had no relationship to histological type, tumor differentiation or lymph node metastasis, but LOH positive rate in TNM Ⅲ＋ Ⅳ was 33.33% （6/18）, much higher than that in TNM Ⅰ ＋ Ⅱ （ 5.26%, 1/19）, （P 〈 0.05）. BRCA1 protein was expressed in 14 of 37 samples of gastric cancer. The positive rates of BRCA1 protein in TNM Ⅰ ＋ Ⅱ and TNM Ⅲ＋ Ⅳ were 57.89% and 16.67%, respectively, （P 〈 0.05）. The positive rate of BRCA1 protein was 77.78% in high differentiation samples, 30.77% in middle differentiation and 12.50% in lower differentiation samples, （P 〈 0.05）. CONCLUSION： MSI of BRCA1 gene could be used as a molecular marker in early phases of sporadic gastric cancer in Chinese population. LOH occurs at later period of gastric cancer, therefore, it could be used as prognostic factor.
基金the National Science and Technology Major Project of the Ministry of Science and Technology of China,No.2018ZX10715-003.
文摘BACKGROUND Pulmonary alveolar proteinosis(PAP)is a rare lung disease characterized by the accumulation of phospholipoproteinaceous material in the alveoli.Cases of PAP complicated with tuberculosis are much more complex and have rarely been well recorded.CASE SUMMARY We describe a 21-year-old Han Chinese patient with suspicious lung infection associated with mild restrictive ventilatory dysfunction and diffusion reduction.High resolution computed tomography revealed a“crazy-paving”appearance and multiple pulmonary miliary nodules around the bronchi.Bronchoalveolar lavage demonstrated a small amount of periodic acid-Schiff positive proteinaceous materials.A serological test for the presence of a Mycobacterium tuberculosis antibody and an interferon-gamma release assay were both positive.The patient received a standard course of first-line anti-tuberculosis treatment after diagnostic bronchoalveolar lavage.To date,clinical remission has been achieved and maintained for five years.CONCLUSION In summary,the diagnosis of PAP complicated with tuberculosis was supported by a combination of clinical manifestations,imaging,pulmonary function,laboratory examinations,bronchoalveolar lavage,etc.This case highlighted that diagnostic bronchoalveolar lavage in combination with anti-tuberculosis treatment is a safe and effective option for mild PAP patients with tuberculosis.
文摘Spontaneous rupture of the iliac vein is rare clinical emergency. Sudden onset, hypertension, and abdominal distention with a nonpulsatile mass in the iliac fossa are the chief symptoms. We reported another case of spontaneous rupture of the left external iliac vein diagnosed intra-operatively and successfully treated with direct suturing. And related literatures were reviewed. Possible etiology and optimal treatment were discussed.
基金National Natural Science Funds for Distinguished Young Scholar (No. 70825004)Creative Research Groups of China (No. 10721101)+1 种基金Shanghai University of Finance and Economics Project 211 Phase ⅢShanghai Leading Academic Discipline Project (No. B803)
文摘Length-biased data arise in many important fields, including epidemiological cohort studies, cancer screening trials and labor economics. Analysis of such data has attracted much attention in the literature. In this paper we propose a quantile regression approach for analyzing right-censored and length-biased data. We derive an inverse probability weighted estimating equation corresponding to the quantile regression to correct the bias due to length-bias sampling and informative censoring. This method can easily handle informative censoring induced by length-biased sampling. This is an appealing feature of our proposed method since it is generally difficult to obtain unbiased estimates of risk factors in the presence of length-bias and informative censoring. We establish the consistency and asymptotic distribution of the proposed estimator using empirical process techniques. A resampling method is adopted to estimate the variance of the estimator. We conduct simulation studies to evaluate its finite sample performance and use a real data set to illustrate the application of the proposed method.
文摘Wernicke encephalopathy(WE)is an acute or subacute neurological syndrome caused by thiamine(Vitamin B 1)deficiency and is usually underestimated in clinical practice.WE is suspected in merely about 6%of non-alcoholic patients and one-third of alcoholic patients,Non-alcoholic causes include gastrointestinal surgery and disease,malnutrition,cancer and chemotherapeutic treatments,and long-term parenteral nutrition.However,few cases were reported about WE in patients with tuberculosis.Early diagnosis and medication are vital for the prognosis of this disease.
