Colorectal cancer(CRC)is a prevalent malignancy characterized by rising incidence and mortality rates worldwide.The involvement of RNA pseudouridine synthase enzymes in CRC development is well-documented,yet the trans...Colorectal cancer(CRC)is a prevalent malignancy characterized by rising incidence and mortality rates worldwide.The involvement of RNA pseudouridine synthase enzymes in CRC development is well-documented,yet the transcriptomic profile of pseudouridine(Ψ)in CRC remains largely unexplored.This study explored the transcriptomic landscape of Ψ in CRC by analyzing 21 biopsies and their corresponding adjacent healthy tissues,along with blood samples from 10 patients using PRAISE,a quantitative pseudouridine sequencing technology at base resolution.Our findings revealed significant differences in Ψ distribution and levels between CRC samples and adjacent normal tissues,correlating with the expression levels of DKC1,PUS7L,and PUS10.Notably,distinct Ψ levels of sno RNAs could serve as potential tumor biomarkers.Furthermore,we assessed the clinical utility of Ψ in both tumor and blood samples.Differentiated Ψ levels showed promising correlations with clinical markers such as cancer antigen 125,cancer antigen 153,and cancer antigen 199 in tumors,whereas Ψ sites in blood revealed enhanced correlations with routine blood indicators like white blood cells(WBCs)and alpha-fetoprotein(AFP).These findings underscore the significant role of RNA Ψ in CRC,providing valuable insights into its potential applications for clinical diagnosis and treatment.展开更多
基金supported by the National Natural Science Foundation of China(22377096,92153303,21721005,91940000,92253202 and 22177087)the Ministry of Science and Technology of China(2023YFC3402200)+2 种基金China Postdoctoral Science Foundation(2023M732692)Fundamental Research Funds for the Central Universities(2042023kf0118 and 2042023kfyq05)Translational Medicine and Interdisciplinary Research Joint Foundation of Zhongnan Hospital of Wuhan University(ZNJC20222)。
文摘Colorectal cancer(CRC)is a prevalent malignancy characterized by rising incidence and mortality rates worldwide.The involvement of RNA pseudouridine synthase enzymes in CRC development is well-documented,yet the transcriptomic profile of pseudouridine(Ψ)in CRC remains largely unexplored.This study explored the transcriptomic landscape of Ψ in CRC by analyzing 21 biopsies and their corresponding adjacent healthy tissues,along with blood samples from 10 patients using PRAISE,a quantitative pseudouridine sequencing technology at base resolution.Our findings revealed significant differences in Ψ distribution and levels between CRC samples and adjacent normal tissues,correlating with the expression levels of DKC1,PUS7L,and PUS10.Notably,distinct Ψ levels of sno RNAs could serve as potential tumor biomarkers.Furthermore,we assessed the clinical utility of Ψ in both tumor and blood samples.Differentiated Ψ levels showed promising correlations with clinical markers such as cancer antigen 125,cancer antigen 153,and cancer antigen 199 in tumors,whereas Ψ sites in blood revealed enhanced correlations with routine blood indicators like white blood cells(WBCs)and alpha-fetoprotein(AFP).These findings underscore the significant role of RNA Ψ in CRC,providing valuable insights into its potential applications for clinical diagnosis and treatment.
