Epilepsy is a serious neurological disorder;however,the effectiveness of current medications is often suboptimal.Recently,stem cell technology has demonstrated remarkable therapeutic potential in addressing various ne...Epilepsy is a serious neurological disorder;however,the effectiveness of current medications is often suboptimal.Recently,stem cell technology has demonstrated remarkable therapeutic potential in addressing various neurological diseases,igniting interest in its applicability for epilepsy treatment.This comprehensive review summarizes different therapeutic approaches utilizing various types of stem cells.Preclinical experiments have explored the use and potential therapeutic effects of mesenchymal stem cells,including genetically modified variants.Clinical trials involving patientderived mesenchymal stem cells have shown promising results,with reductions in the frequency of epileptic seizures and improvements in neurological,cognitive,and motor functions reported.Another promising therapeutic strategy involves neural stem cells.These cells can be cultured outside the body and directed to differentiate into specific cell types.The transplant of neural stem cells has the potential to replace lost inhibitory interneurons,providing a novel treatment avenue for epilepsy.Embryonic stem cells are characterized by their significant capacity for self-renewal and their ability to differentiate into any type of somatic cell.In epilepsy treatment,embryonic stem cells can serve three primary functions:neuron regeneration,the maintenance of cellular homeostasis,and restorative activity.One notable strategy involves differentiating embryonic stem cells intoγ-aminobutyric acidergic neurons for transplantation into lesion sites.This approach is currently undergoing clinical trials and could be a breakthrough in the treatment of refractory epilepsy.Induced pluripotent stem cells share the same genetic background as the donor,thereby reducing the risk of immune rejection and addressing ethical concerns.However,research on induced pluripotent stem cell therapy remains in the preclinical stage.Despite the promise of stem cell therapies for epilepsy,several limitations must be addressed.Safety concerns persist,including issues such as tumor formation,and the low survival rate of transplanted cells remains a significant challenge.Additionally,the high cost of these treatments may be prohibitive for some patients.In summary,stem cell therapy shows considerable promise in managing epilepsy,but further research is needed to overcome its existing limitations and enhance its clinical applicability.展开更多
To address the performance limitations of conventional LiPF6-carbonate electrolytes under extreme temperatures and high-rate charging,lithium difluoro(oxalato)borate(LiDFOB)is introduced into the LiPF6-carbonate elect...To address the performance limitations of conventional LiPF6-carbonate electrolytes under extreme temperatures and high-rate charging,lithium difluoro(oxalato)borate(LiDFOB)is introduced into the LiPF6-carbonate electrolyte to form a dual-salt system.The optimization mechanism enhancing the fast-charging capability of LiNi_(0.52)Co_(0.2)Mn_(0.28)O_(2)(NCM523)cathode is systematically explored.Molecular dynamics simulations and electrochemical characterization demonstrate the reconstruction of Li+solvation structures,expanding the voltage window and reducting Li^(+)desolvation barriers.In addition,the incorporation of LiDFOB induces the generation of a LiF/Li_(x)BO_(y)F_(z)-enriched cathode-electrolyte interphase,which effectively suppresses the dissolution of transition metals.In situ impedance measurements reveal the accelerated interfacial charge transfer kinetics.As expected,the NCM523 cathode achieves an 82%state-of-charge(SOC)in 12 min at 5 C(25°C)with 87%capacity retention after 100 cycles,and exhibits a 65%higher discharge capacity at 1 C than the baseline at−20°C.The 1 Ah pouch cells based on LiNi_(0.52)Co_(0.2)Mn_(0.28)O_(2)cathodes,graphite anodes,and 0.5 wt%LiDFOB-modified electrolyte demonstrate fast-charging capabilities:charging 97%of the pouch cell capacity within 30 min(2 C)and 80%within 15 min(4 C)at 25°C.This study offers a practical electrolyte design strategy that enhances the fast-charging performance of lithium-ion batteries(LIBs)over a wide temperature range(from−20 to 25°C).展开更多
The rapidly growing demand for lithium iron phosphate(LiFePO_(4))as the cathode material of lithium-ion batteries(LIBs)has aggravated the scarcity of phosphorus(P)reserves on Earth.This study introduces an environment...The rapidly growing demand for lithium iron phosphate(LiFePO_(4))as the cathode material of lithium-ion batteries(LIBs)has aggravated the scarcity of phosphorus(P)reserves on Earth.This study introduces an environmentally friendly and economical method of P recovery from municipal wastewater,providing the P source for LiFePO_(4) cathodes.The novel approach utilizes the sludge of Fe-coagulant-based chemical P removal(CPR)in wastewater treatment.After a sintering treatment with acid washing,the CPR sludge,enriched with P and Fe,transforms into purified P-Fe oxides(Fe2.1P1.0O5.6).These oxides can substitute up to 35%of the FePO_(4) reagent as precursor,producing a carbon-coated LiFePO_(4)(LiFePO_(4)/C)cathode with a specific discharge capacity of 114.9 mA·h·g^(-1)at current density of 17 mA·g^(-1)),and cycle stability of 99.2%after 100 cycles.The enhanced cycle performance of the as-prepared LiFePO_(4)/C cathode may be attributed to the incorporations of impurities(such as Ca^(2+)and Na^(+))from sludge,with improved stability of crystal structure.Unlike conventional P-fertilizers,this P recovery technology converts 100%of P in CPR sludge into the production of value-added LiFePO_(4)/C cathodes.The recovered P from municipal wastewater can meet up to 35%of the P demand in the Chinese LIBs industry,offering a cost-effective solution for addressing the pressing challenges of P scarcity.展开更多
BACKGROUND Rosmarinic acid(RA)is a natural polyphenol carboxylic acid known for its role in chemoprevention.Given its widespread use as a food additive,we are interested in whether RA affects the development of colore...BACKGROUND Rosmarinic acid(RA)is a natural polyphenol carboxylic acid known for its role in chemoprevention.Given its widespread use as a food additive,we are interested in whether RA affects the development of colorectal cancer(CRC).AIM To examine the anti-tumor effects of RA on various CRC cell lines,and to further investigate the possible mechanisms.METHODS Cell Counting Kit-8 assay and optical microscopy imaging were used to evaluate the viability of CRC cell lines.Western blot,quantitative real-time polymerase chain reaction,and flow cytometry analyses were performed to assess cell viability and activation of nuclear factor-kappa B(NF-κB)signaling.Molecular modeling was used to assess the interaction between RA and inhibitory kappa B kinase beta.Luciferase assay was used to examine the activity of NF-κB-driven transcription.The combinations of RA with 5-fluorouracil or oxaliplatin were utilized to evaluate the potential synergistic action of RA with the chemotherapeutics.RESULTS RA exerted potent cytotoxic actions on all six CRC cell lines examined.RA was docked nicely into the binding pocket of inhibitory kappa B kinase beta by molecular modeling.The activity of NF-κB-driven luciferase and the phosphorylation of NF-κB p65 were decreased after exposure to the compound.Lipopolysaccharide-induced NF-κB activation was effectively inhibited by RA,too.Further,RA downregulated the expression of cell proliferationrelated cyclin D1 and MYC,which are target genes of NF-κB.Of note,the cytotoxic actions of 5-fluorouracil and oxaliplatin were markedly enhanced by RA in those CRC cells.CONCLUSION Our results indicate that RA inhibits NF-κB signaling and induces apoptosis in CRC cells.It enhances the cytotoxic actions of chemotherapeutics and might help to improve the chemotherapy of CRC.展开更多
In-situ polymer electrolytes prepared by Li salt-initiated polymerization are promising electrolytes for solid-state Li metal batteries owing to their enhanced interface contact and facile and green preparation proces...In-situ polymer electrolytes prepared by Li salt-initiated polymerization are promising electrolytes for solid-state Li metal batteries owing to their enhanced interface contact and facile and green preparation process.However,conventional in-situ polymer electrolytes suffer from poor interface stability,low mechanical strength,low oxidation stability,and certain flammability.Herein,a silsesquioxane(POSS)-nanocage-crosslinked in-situ polymer electrolyte(POSS-DOL@PI-F)regulated by fluorinated plasticizer and enhanced by polyimide skeleton is fabricated by Li salt initiated in-situ polymerization.Polyimide skeleton and POSS-nanocage-crosslinked network significantly enhance the tensile strength(22.8 MPa)and thermal stability(200℃)of POSS-DOL@PI-F.Fluorinated plasticizer improves ionic conductivity(6.83×10^(-4)S cm^(-1)),flame retardance,and oxidation stability(5.0 V)of POSS-DOL@PI-F.The fluorinated plasticizer of POSS-DOL@PI-F constructs robust LiF-rich solid electrolyte interphases and cathode electrolyte interphases,thereby dramatically enhancing the interface stability of Li metal anodes and LiNi_(0.8)Mn_(0.1)Co_(0.1)O_(2)(NCM811)cathodes.POSS-DOL@PI-F enables stable,long-term(1200 h),and dendrite-free cycle of Li‖Li cells.POSS-DOL@PI-F significantly boosts the performance of Li‖NCM811cells,which display superior cycle stability under harsh conditions of high voltage(4.5 V),high temperature(60℃),low temperature(-20℃),and high areal capacity.This work provides a rational design strategy for safe and efficient polymer electrolytes.展开更多
Model-free,data-driven prediction of chaotic motions is a long-standing challenge in nonlinear science.Stimulated by the recent progress in machine learning,considerable attention has been given to the inference of ch...Model-free,data-driven prediction of chaotic motions is a long-standing challenge in nonlinear science.Stimulated by the recent progress in machine learning,considerable attention has been given to the inference of chaos by the technique of reservoir computing(RC).In particular,by incorporating a parameter-control channel into the standard RC,it is demonstrated that the machine is able to not only replicate the dynamics of the training states,but also infer new dynamics not included in the training set.The new machine-learning scheme,termed parameter-aware RC,opens up new avenues for data-based analysis of chaotic systems,and holds promise for predicting and controlling many real-world complex systems.Here,using typical chaotic systems as examples,we give a comprehensive introduction to this powerful machine-learning technique,including the algorithm,the implementation,the performance,and the open questions calling for further studies.展开更多
Ferroptosis is a novel form of non-apoptotic cell death that has been widely studied in recent years and is involved in a variety of pathophysiological processes.The core treatment goal of ischemic stroke is to restor...Ferroptosis is a novel form of non-apoptotic cell death that has been widely studied in recent years and is involved in a variety of pathophysiological processes.The core treatment goal of ischemic stroke is to restore blood flow as early as possible,while the pathological mechanism of reperfusion injury after restoring blood flow is complex,involving oxidative stress,calcium overload,and inflammatory response.In recent years,more and more studies have found that ferroptosis mediation is involved in the occurrence and development of cerebral ischemia-reperfusion injury.This paper elaborates on the concept,mechanisms,and regulation of ferroptosis,detailing its role in cerebral ischemia-reperfusion injury and potential inhibition strategies.The aim is to deepen the understanding of ferroptosis in this pathological process and provide insights for possible targeted therapies.展开更多
van der Waals(vdW)heterostructures constructed by lowdimensional(0D,1D,and 2D)materials are emerging as one of the most appealing systems in next-generation flexible photodetection.Currently,hand-stacked vdW-type phot...van der Waals(vdW)heterostructures constructed by lowdimensional(0D,1D,and 2D)materials are emerging as one of the most appealing systems in next-generation flexible photodetection.Currently,hand-stacked vdW-type photodetectors are not compatible with large-areaarray fabrication and show unimpressive performance in self-powered mode.Herein,vertical 1D GaN nanorods arrays(NRAs)/2D MoS_(2)/PEDOT:PSS in wafer scale have been proposed for self-powered flexible photodetectors arrays firstly.The as-integrated device without external bias under weak UV illumination exhibits a competitive responsivity of 1.47 A W^(−1)and a high detectivity of 1.2×10^(11)Jones,as well as a fast response speed of 54/71μs,thanks to the strong light absorption of GaN NRAs and the efficient photogenerated carrier separation in type-II heterojunction.Notably,the strain-tunable photodetection performances of device have been demonstrated.Impressively,the device at−0.78%strain and zero bias reveals a significantly enhanced photoresponse with a responsivity of 2.47 A W^(−1),a detectivity of 2.6×10^(11)Jones,and response times of 40/45μs,which are superior to the state-of-the-art self-powered flexible photodetectors.This work presents a valuable avenue to prepare tunable vdWs heterostructures for self-powered flexible photodetection,which performs well in flexible sensors.展开更多
Urban areas face environmental pollution and greenhouse emissions challenges,demand collaborative efforts to mitigation.Urban forests play a crucial role in absorbing CO_(2) emissions and contributing to carbon seques...Urban areas face environmental pollution and greenhouse emissions challenges,demand collaborative efforts to mitigation.Urban forests play a crucial role in absorbing CO_(2) emissions and contributing to carbon sequestration potential,but they also release biogenic volatile organic compounds(BVOCs),which contribute to the formation of tropospheric ozone and secondary organic aerosols(SOA).This study aimed to understand the role of urban forests in carbon stock and BVOCs emission by establishing optimal biomass models for six typical tree species(Robinia pseudoacacia,Quercus,Populus,Pinus tabulaeformis,Betula platyphylla,and Larix gmelinii)in Beijing.Biomass models were developed using field surveys and remote sensing data,with R^(2) values ranging from 0.364 to 0.921.Applying these models to forest resource inventory data,carbon stock and BVOCs emission models were constructed.In 2021,the total carbon stock for these pure forest tree species was estimated at 5.638 million tons,with a carbon density of 58.86 t/ha.The carbon density ranking for pure forest tree species was:Robinia pseudoacacia>Populus tomentosa>Betula platyphylla>Quercus Linn>Pinus tabulaeformis>Larix gmelinii.Total BVOCs emission in 2021 from the studied species were calculated at 25,789.72 t,with an average emission of 0.27 t/ha.Populus tomentosa had the highest BVOCs emission per unit area,followed by Robinia pseudoacacia,and Larix gmelinii had the smallest.Betula platyphylla and Robinia pseudoacacia were identified as species with high carbon stock and low BVOCs emissions in Beijing,offering insights for future urban forest planning and eco-friendly urban environment development strategies.展开更多
BACKGROUND Electroacupuncture(EA)has been recognized for its beneficial effects on glucolipid metabolism,potentially through the regulation of sensory nerve coordination.The expandability of peripancreatic adipose tis...BACKGROUND Electroacupuncture(EA)has been recognized for its beneficial effects on glucolipid metabolism,potentially through the regulation of sensory nerve coordination.The expandability of peripancreatic adipose tissue(PAT)is implicated in the transition from obesity to type 2 diabetes mellitus(T2DM).However,the specific pancreatic responses to EA require further elucidation.AIM To investigate the influence of EA on pancreatic glucolipid reduction level in a high-fat diet(HFD)rat model.METHODS To delineate the precise pathway through which EA mediates interactions bet ween PAT and islets,we assessed the expression levels of NGF,TRPV1,insulin,as well as other proteins in the pancreas and PAT.This approach enabled us to identify the acupoints that are most conducive to optimizing glycolipid metabolism.RESULTS The ST25,LI11 and ST37 groups attenuated HFD-induced obesity and insulin resistance(IR)to distinct degrees,with ST25 group having the greatest effect.EA at ST25 was found to modify the local regulatory influence of PAT on the pancreatic intrinsic nervous system.Specifically,EA at ST25 obviously activated the TRPV1-CGRP-islet beta cell pathway,contributing to the relief of glucolipid metabolic stress.The beneficial effects were abrogated following the chemical silencing of TRPV1 sensory afferents,confirming their indispensable role in EA-mediated regulation of islet and PAT function.Furthermore,in TRPV1 knockout mice,a reduction in PAT inflammation was observed,along with the recovery of islet beta cell function.EA at LI11 and ST37 demonstrated anti-inflammatory properties and helped ameliorate IR.CONCLUSION The PAT ecological niche influenced the progression from obesity to T2DM through various immunometabolic pathways.EA at ST25 could regulate glucolipid metabolism via the TRPV1-CGRP-islet beta cell pathway.展开更多
Emerging evidence highlights the role of thyroid hormones in cancer,although findings are controversial.Research on thyroid-related traits in lung carcinogenesis is limited.Using UK Biobank data,we performed bidirecti...Emerging evidence highlights the role of thyroid hormones in cancer,although findings are controversial.Research on thyroid-related traits in lung carcinogenesis is limited.Using UK Biobank data,we performed bidirectional Mendelian randomization(MR)to assess causal associations between lung cancer risk and thyroid dysfunction(hypothyroidism and hyperthyroidism)or functional traits(free thyroxine[FT4]and normal-range thyroid-stimulating hormone[TSH]).Furthermore,in the smoking-behavior-stratified MR analysis,we evaluated the mediating effect of thyroid-related phenotypes on the association between smoking behaviors and lung cancer.We demonstrated significant associations between lung cancer risk and hypothyroidism(hazard ratio[HR]=1.14,95%confidence interval[CI]=1.03–1.26,P=0.009)and hyperthyroidism(HR=1.55,95%CI=1.29–1.87,P=1.90×10^(-6))in the UKB.Moreover,the MR analysis indicated a causal effect of thyroid dysfunction on lung cancer risk(ORinverse variance weighted[IVW]=1.09,95%CI=1.05–1.13,P=3.12×10^(-6)for hypothyroidism;ORIVW=1.08,95%CI=1.04–1.12,P=8.14×10^(-5)for hyperthyroidism).We found that FT4 levels were protective against lung cancer risk(ORIVW=0.93,95%CI=0.87–0.99,P=0.030).Additionally,the stratified MR analysis demonstrated distinct causal effects of thyroid dysfunction on lung cancer risk among smokers.Hyperthyroidism mediated the effect of smoking behaviors,especially the age of smoking initiation(17.66%mediated),on lung cancer risk.Thus,thyroid dysfunction phenotypes play causal roles in lung cancer development exclusively among smokers and act as mediators in the causal pathway from smoking to lung cancer.展开更多
Perovskite oxides(ABO_(3))are thought to be promising electrocatalysts for oxygen evolution reaction(OER),but their specific surface area(SSA)is too low(usually<10 m^(2) g^(−1)).Developing advanced ABO_(3) electroc...Perovskite oxides(ABO_(3))are thought to be promising electrocatalysts for oxygen evolution reaction(OER),but their specific surface area(SSA)is too low(usually<10 m^(2) g^(−1)).Developing advanced ABO_(3) electrocatalysts with high SSA and optimized structure is of great significance but remains a tremendous challenge.Herein,we propose a general strategy for fabrication of mesoporous perovskite oxide nanosheets(MPONs)with controllable atomic doping via self-sacrificial template-induced nanostructure modulation.A variety of MPONs including LaFeO_(3),A-site-doped LaFeO_(3)(A-LaFeO_(3),where A is Pr,Nd,Sm,Eu,or Gd)and B-site-doped LaFeO_(3)(B-LaFeO_(3),where B is Mn,Co,Ni,Cu,or Zn)have been achieved.Interestingly,it is discovered that the catalytic activities of A-LaFeO_(3) MPONs as OER catalysts are overall higher than those of B-LaFeO_(3) ones.Especially,the screened Eu-LaFeO_(3) MPONs only require a low overpotential of 267 mV at 10 mA cm^(−2),outperforming most reported perovskite oxides.The superior catalytic activity of Eu-LaFeO_(3) MPONs is attributed to their favorable porous structure,which increases the density of active sites,and enhanced lattice oxygen participation,which improves the intrinsic activity.This study provides guidance for the design and controlled synthesis of advanced rare-earth-doped MPONs with ultrahigh SSA for enhanced electrocatalysis.展开更多
Mn-based P2-type oxides are considered as promising cathodes for Na-ion batteries;however,they face significant challenges,including structural degradation when charged at high cutoff voltages and structural changes u...Mn-based P2-type oxides are considered as promising cathodes for Na-ion batteries;however,they face significant challenges,including structural degradation when charged at high cutoff voltages and structural changes upon storing in a humid atmosphere.In response to these issues,we have designed an oxide with co-doping of Cu and Al which can balance both cost and structural stability.The redox reaction of Cu^(2+/3+)can provide certain charge compensation,and the introduction of Al can further suppress the Jahn-Teller effect of Mn,thereby achieving superior long-term cycling performance.The ex-situ XRD testing indicates that Cu/Al co-doping can effectively suppress the phase transition of P2-O2 at high voltage,thereby explaining the improvement in electrochemical performance.DFT calculations reveal a high chemical tolerance to moisture,with lower adsorption energy for H_(2)O compared to pure Na_(0.67)Cu_(0.25)Mn_(0.75)O_(2).A representative Na_(0.67)Cu_(0.20)Al_(0.05)Mn_(0.75)O_(2)cathode demonstrates impressive reversible capacities of 148.7 mAh/g at 0.2 C,along with a remarkable capacity retention of 79.1%(2 C,500 cycles).展开更多
BACKGROUND Although extensive research has investigated attentional biases based on the looming vulnerability model of anxiety,the characteristics of attentional biases in individuals with looming cognitive styles(LCS...BACKGROUND Although extensive research has investigated attentional biases based on the looming vulnerability model of anxiety,the characteristics of attentional biases in individuals with looming cognitive styles(LCS)remain incompletely elucidated.No prior eye-tracking studies have examined the spatiotemporal dynamics of their threat-related attentional preferences.AIM To investigate the nature and temporal pattern of attentional biases toward threat stimuli in individuals exhibiting different levels of LCS using eye-tracking technology.METHODS A total of 212 participants were stratified according to their Looming Maladaptive Style Questionnaire scores.From the high and low scoring subgroups,35 participants were randomly selected for an eye-tracking experiment using a classic dot-probe paradigm featuring threat and neutral images.Four eye-tracking metrics,including first fixation latency,first fixation duration,total fixation duration,and fixation count,were analyzed to assess detection speed,attentional orienting,initial maintenance/avoidance,and overall engagement.RESULTS Distinct attentional bias patterns were observed between high and low LCS groups.High LCS individuals exhibited a vigilance-avoidance pattern characterized by initial vigilance toward threat stimuli(evidenced by faster detection and preferential orienting),followed by attentional avoidance,alongside sustained attention maintenance to threat.CONCLUSION These findings reveal a temporal dissociation between early vigilance and later avoidance during threat processing in high LCS individuals,providing novel empirical evidence to refine models of cognitive vulnerability and attentional dynamics in threat perception.展开更多
BACKGROUND Peripheral neuropathy caused by diabetes is closely related to the vicious cycle of oxidative stress and mitochondrial dysfunction resulting from metabolic abnormalities.The effects mediated by the silent i...BACKGROUND Peripheral neuropathy caused by diabetes is closely related to the vicious cycle of oxidative stress and mitochondrial dysfunction resulting from metabolic abnormalities.The effects mediated by the silent information regulator type 2 homolog-1(SIRT1)/peroxisome proliferator-activated receptor-gamma coactivator-1α(PGC-1α)axis present new opportunities for the treatment of type 2 diabetic peripheral neuropathy(T2DPN),potentially breaking this harmful cycle.AIM To validate the effectiveness of electroacupuncture(EA)in the treatment of T2DPN and investigate its potential mechanism based on the SIRT1/PGC-1αaxis.METHODS The effects of EA were evaluated through assessments of metabolic changes,morphological observations,and functional examinations of the sciatic nerve,along with measurements of inflammation and oxidative stress.Proteins related to the SIRT1/PGC-1αaxis,involved in the regulation of mitochondrial biogenesis and antioxidative stress,were detected in the sciatic nerve using Western blotting to explain the underlying mechanism.A counterevidence group was created by injecting a SIRT1 inhibitor during EA intervention to support the hypothesis.RESULTS In addition to diabetes-related metabolic changes,T2DPN rats showed significant reductions in pain threshold after 9 weeks,suggesting abnormal peripheral nerve function.EA treatment partially restored metabolic control and reduced nerve damage in T2DPN rats.The SIRT1/PGC-1αaxis,which was downregulated in the model group,was upregulated by EA intervention.The endogenous antioxidant system related to the SIRT1/PGC-1αaxis,previously inhibited in diabetic rats,was reactivated.A similar trend was observed in inflammatory markers.When SIRT1 was inhibited in diabetic rats,these beneficial effects were abolished.CONCLUSION EA can alleviate the symptoms of T2DNP in experimental rats,and its effects may be related to the mitochondrial biogenesis and endogenous antioxidant system mediated by the SIRT1/PGC-1αaxis.展开更多
基金supported by the National Natural Science Foundation of China,Nos.82471471(to WJ),82471485(to FY)Shaanxi Province Special Support Program for Leading Talents in Scientific and Technological Innovation,No.tzjhjw(to WJ)+1 种基金Shaanxi Key Research and Development Plan Project,No.2023-YBSF-353(to XW)the Joint Fund Project of Innovation Research Institute of Xijing Hospital,No.LHJJ24JH13(to ZS)。
文摘Epilepsy is a serious neurological disorder;however,the effectiveness of current medications is often suboptimal.Recently,stem cell technology has demonstrated remarkable therapeutic potential in addressing various neurological diseases,igniting interest in its applicability for epilepsy treatment.This comprehensive review summarizes different therapeutic approaches utilizing various types of stem cells.Preclinical experiments have explored the use and potential therapeutic effects of mesenchymal stem cells,including genetically modified variants.Clinical trials involving patientderived mesenchymal stem cells have shown promising results,with reductions in the frequency of epileptic seizures and improvements in neurological,cognitive,and motor functions reported.Another promising therapeutic strategy involves neural stem cells.These cells can be cultured outside the body and directed to differentiate into specific cell types.The transplant of neural stem cells has the potential to replace lost inhibitory interneurons,providing a novel treatment avenue for epilepsy.Embryonic stem cells are characterized by their significant capacity for self-renewal and their ability to differentiate into any type of somatic cell.In epilepsy treatment,embryonic stem cells can serve three primary functions:neuron regeneration,the maintenance of cellular homeostasis,and restorative activity.One notable strategy involves differentiating embryonic stem cells intoγ-aminobutyric acidergic neurons for transplantation into lesion sites.This approach is currently undergoing clinical trials and could be a breakthrough in the treatment of refractory epilepsy.Induced pluripotent stem cells share the same genetic background as the donor,thereby reducing the risk of immune rejection and addressing ethical concerns.However,research on induced pluripotent stem cell therapy remains in the preclinical stage.Despite the promise of stem cell therapies for epilepsy,several limitations must be addressed.Safety concerns persist,including issues such as tumor formation,and the low survival rate of transplanted cells remains a significant challenge.Additionally,the high cost of these treatments may be prohibitive for some patients.In summary,stem cell therapy shows considerable promise in managing epilepsy,but further research is needed to overcome its existing limitations and enhance its clinical applicability.
基金financially supported by the National Natural Science Foundation of China (Grant No. 52372191)the National Natural Science Foundation of China (Grant No. 22271106)+2 种基金the National Science Foundation of China (Grant Nos. 52073286 (C.-Z.L.), 22275185 (C.-Z.L.))the Fujian Science&Technology Innovation Laboratory for Optoelectronic Information of China(2021ZZ115 (C.-Z.L.)the XMIREM Autonomously Deployment Project (2023GG01 (C.-Z.L.))
文摘To address the performance limitations of conventional LiPF6-carbonate electrolytes under extreme temperatures and high-rate charging,lithium difluoro(oxalato)borate(LiDFOB)is introduced into the LiPF6-carbonate electrolyte to form a dual-salt system.The optimization mechanism enhancing the fast-charging capability of LiNi_(0.52)Co_(0.2)Mn_(0.28)O_(2)(NCM523)cathode is systematically explored.Molecular dynamics simulations and electrochemical characterization demonstrate the reconstruction of Li+solvation structures,expanding the voltage window and reducting Li^(+)desolvation barriers.In addition,the incorporation of LiDFOB induces the generation of a LiF/Li_(x)BO_(y)F_(z)-enriched cathode-electrolyte interphase,which effectively suppresses the dissolution of transition metals.In situ impedance measurements reveal the accelerated interfacial charge transfer kinetics.As expected,the NCM523 cathode achieves an 82%state-of-charge(SOC)in 12 min at 5 C(25°C)with 87%capacity retention after 100 cycles,and exhibits a 65%higher discharge capacity at 1 C than the baseline at−20°C.The 1 Ah pouch cells based on LiNi_(0.52)Co_(0.2)Mn_(0.28)O_(2)cathodes,graphite anodes,and 0.5 wt%LiDFOB-modified electrolyte demonstrate fast-charging capabilities:charging 97%of the pouch cell capacity within 30 min(2 C)and 80%within 15 min(4 C)at 25°C.This study offers a practical electrolyte design strategy that enhances the fast-charging performance of lithium-ion batteries(LIBs)over a wide temperature range(from−20 to 25°C).
基金supported by the National Natural Science Foundation of China(52100093,52270128,and 52261135627)the Guangdong Basic and Applied Basic Research Foundation(2023A1515011734 and 2021B1515120068)+2 种基金the Municipal Science and Technology Innovation Council of the Shen-zhen Government(KCXFZ20211020163556020 and SGDX20230116092359002)the Research Grants Council(17210219)the Innovation and Technology Fund(ITS/242/20FP)of the Hong Kong SAR Government。
文摘The rapidly growing demand for lithium iron phosphate(LiFePO_(4))as the cathode material of lithium-ion batteries(LIBs)has aggravated the scarcity of phosphorus(P)reserves on Earth.This study introduces an environmentally friendly and economical method of P recovery from municipal wastewater,providing the P source for LiFePO_(4) cathodes.The novel approach utilizes the sludge of Fe-coagulant-based chemical P removal(CPR)in wastewater treatment.After a sintering treatment with acid washing,the CPR sludge,enriched with P and Fe,transforms into purified P-Fe oxides(Fe2.1P1.0O5.6).These oxides can substitute up to 35%of the FePO_(4) reagent as precursor,producing a carbon-coated LiFePO_(4)(LiFePO_(4)/C)cathode with a specific discharge capacity of 114.9 mA·h·g^(-1)at current density of 17 mA·g^(-1)),and cycle stability of 99.2%after 100 cycles.The enhanced cycle performance of the as-prepared LiFePO_(4)/C cathode may be attributed to the incorporations of impurities(such as Ca^(2+)and Na^(+))from sludge,with improved stability of crystal structure.Unlike conventional P-fertilizers,this P recovery technology converts 100%of P in CPR sludge into the production of value-added LiFePO_(4)/C cathodes.The recovered P from municipal wastewater can meet up to 35%of the P demand in the Chinese LIBs industry,offering a cost-effective solution for addressing the pressing challenges of P scarcity.
基金Supported by Natural Science Foundation of Heilongjiang Province of China Under Grant,No.PL2024H020High-Quality Innovation Platform of Science and Education Innovation Zone in Suzhou Industrial Park-Key Platform Project,No.YZCXPT2023104.
文摘BACKGROUND Rosmarinic acid(RA)is a natural polyphenol carboxylic acid known for its role in chemoprevention.Given its widespread use as a food additive,we are interested in whether RA affects the development of colorectal cancer(CRC).AIM To examine the anti-tumor effects of RA on various CRC cell lines,and to further investigate the possible mechanisms.METHODS Cell Counting Kit-8 assay and optical microscopy imaging were used to evaluate the viability of CRC cell lines.Western blot,quantitative real-time polymerase chain reaction,and flow cytometry analyses were performed to assess cell viability and activation of nuclear factor-kappa B(NF-κB)signaling.Molecular modeling was used to assess the interaction between RA and inhibitory kappa B kinase beta.Luciferase assay was used to examine the activity of NF-κB-driven transcription.The combinations of RA with 5-fluorouracil or oxaliplatin were utilized to evaluate the potential synergistic action of RA with the chemotherapeutics.RESULTS RA exerted potent cytotoxic actions on all six CRC cell lines examined.RA was docked nicely into the binding pocket of inhibitory kappa B kinase beta by molecular modeling.The activity of NF-κB-driven luciferase and the phosphorylation of NF-κB p65 were decreased after exposure to the compound.Lipopolysaccharide-induced NF-κB activation was effectively inhibited by RA,too.Further,RA downregulated the expression of cell proliferationrelated cyclin D1 and MYC,which are target genes of NF-κB.Of note,the cytotoxic actions of 5-fluorouracil and oxaliplatin were markedly enhanced by RA in those CRC cells.CONCLUSION Our results indicate that RA inhibits NF-κB signaling and induces apoptosis in CRC cells.It enhances the cytotoxic actions of chemotherapeutics and might help to improve the chemotherapy of CRC.
基金supported by the National Natural Science Foundation of China(22375116,22001057)the Science Foundation of High-Level Talents of Wuyi University(2019AL017,2021AL002)Tianjin Lishen Battery Co.,Ltd。
文摘In-situ polymer electrolytes prepared by Li salt-initiated polymerization are promising electrolytes for solid-state Li metal batteries owing to their enhanced interface contact and facile and green preparation process.However,conventional in-situ polymer electrolytes suffer from poor interface stability,low mechanical strength,low oxidation stability,and certain flammability.Herein,a silsesquioxane(POSS)-nanocage-crosslinked in-situ polymer electrolyte(POSS-DOL@PI-F)regulated by fluorinated plasticizer and enhanced by polyimide skeleton is fabricated by Li salt initiated in-situ polymerization.Polyimide skeleton and POSS-nanocage-crosslinked network significantly enhance the tensile strength(22.8 MPa)and thermal stability(200℃)of POSS-DOL@PI-F.Fluorinated plasticizer improves ionic conductivity(6.83×10^(-4)S cm^(-1)),flame retardance,and oxidation stability(5.0 V)of POSS-DOL@PI-F.The fluorinated plasticizer of POSS-DOL@PI-F constructs robust LiF-rich solid electrolyte interphases and cathode electrolyte interphases,thereby dramatically enhancing the interface stability of Li metal anodes and LiNi_(0.8)Mn_(0.1)Co_(0.1)O_(2)(NCM811)cathodes.POSS-DOL@PI-F enables stable,long-term(1200 h),and dendrite-free cycle of Li‖Li cells.POSS-DOL@PI-F significantly boosts the performance of Li‖NCM811cells,which display superior cycle stability under harsh conditions of high voltage(4.5 V),high temperature(60℃),low temperature(-20℃),and high areal capacity.This work provides a rational design strategy for safe and efficient polymer electrolytes.
基金Project supported by the National Natural Science Foundation of China(Grant No.12275165)XGW was also supported by the Fundamental Research Funds for the Central Universities(Grant No.GK202202003).
文摘Model-free,data-driven prediction of chaotic motions is a long-standing challenge in nonlinear science.Stimulated by the recent progress in machine learning,considerable attention has been given to the inference of chaos by the technique of reservoir computing(RC).In particular,by incorporating a parameter-control channel into the standard RC,it is demonstrated that the machine is able to not only replicate the dynamics of the training states,but also infer new dynamics not included in the training set.The new machine-learning scheme,termed parameter-aware RC,opens up new avenues for data-based analysis of chaotic systems,and holds promise for predicting and controlling many real-world complex systems.Here,using typical chaotic systems as examples,we give a comprehensive introduction to this powerful machine-learning technique,including the algorithm,the implementation,the performance,and the open questions calling for further studies.
基金Natural Science Basic Research Program of Shaanxi Province(Project No.:2021JM-554)。
文摘Ferroptosis is a novel form of non-apoptotic cell death that has been widely studied in recent years and is involved in a variety of pathophysiological processes.The core treatment goal of ischemic stroke is to restore blood flow as early as possible,while the pathological mechanism of reperfusion injury after restoring blood flow is complex,involving oxidative stress,calcium overload,and inflammatory response.In recent years,more and more studies have found that ferroptosis mediation is involved in the occurrence and development of cerebral ischemia-reperfusion injury.This paper elaborates on the concept,mechanisms,and regulation of ferroptosis,detailing its role in cerebral ischemia-reperfusion injury and potential inhibition strategies.The aim is to deepen the understanding of ferroptosis in this pathological process and provide insights for possible targeted therapies.
基金supported by the National Key Research and Development Program of China(No.2022YFB3604500,No.2022YFB3604501)the National Natural Science Foundation of China(No.52172141)the Technology Development Project of Shanxi-Zheda Institude of Advanced Materials and Chemical Engineering(No.2022SX-TD017).
文摘van der Waals(vdW)heterostructures constructed by lowdimensional(0D,1D,and 2D)materials are emerging as one of the most appealing systems in next-generation flexible photodetection.Currently,hand-stacked vdW-type photodetectors are not compatible with large-areaarray fabrication and show unimpressive performance in self-powered mode.Herein,vertical 1D GaN nanorods arrays(NRAs)/2D MoS_(2)/PEDOT:PSS in wafer scale have been proposed for self-powered flexible photodetectors arrays firstly.The as-integrated device without external bias under weak UV illumination exhibits a competitive responsivity of 1.47 A W^(−1)and a high detectivity of 1.2×10^(11)Jones,as well as a fast response speed of 54/71μs,thanks to the strong light absorption of GaN NRAs and the efficient photogenerated carrier separation in type-II heterojunction.Notably,the strain-tunable photodetection performances of device have been demonstrated.Impressively,the device at−0.78%strain and zero bias reveals a significantly enhanced photoresponse with a responsivity of 2.47 A W^(−1),a detectivity of 2.6×10^(11)Jones,and response times of 40/45μs,which are superior to the state-of-the-art self-powered flexible photodetectors.This work presents a valuable avenue to prepare tunable vdWs heterostructures for self-powered flexible photodetection,which performs well in flexible sensors.
基金supported by the National Natural Science Foundation of China(No.42077454)the Xiong'an New Area Science and Technology Innovation Project(No.2022XACX1000)the 5·5 Engineering Research&Innovation Team Project of Beijing Forestry University(No.BLRC2023B04).
文摘Urban areas face environmental pollution and greenhouse emissions challenges,demand collaborative efforts to mitigation.Urban forests play a crucial role in absorbing CO_(2) emissions and contributing to carbon sequestration potential,but they also release biogenic volatile organic compounds(BVOCs),which contribute to the formation of tropospheric ozone and secondary organic aerosols(SOA).This study aimed to understand the role of urban forests in carbon stock and BVOCs emission by establishing optimal biomass models for six typical tree species(Robinia pseudoacacia,Quercus,Populus,Pinus tabulaeformis,Betula platyphylla,and Larix gmelinii)in Beijing.Biomass models were developed using field surveys and remote sensing data,with R^(2) values ranging from 0.364 to 0.921.Applying these models to forest resource inventory data,carbon stock and BVOCs emission models were constructed.In 2021,the total carbon stock for these pure forest tree species was estimated at 5.638 million tons,with a carbon density of 58.86 t/ha.The carbon density ranking for pure forest tree species was:Robinia pseudoacacia>Populus tomentosa>Betula platyphylla>Quercus Linn>Pinus tabulaeformis>Larix gmelinii.Total BVOCs emission in 2021 from the studied species were calculated at 25,789.72 t,with an average emission of 0.27 t/ha.Populus tomentosa had the highest BVOCs emission per unit area,followed by Robinia pseudoacacia,and Larix gmelinii had the smallest.Betula platyphylla and Robinia pseudoacacia were identified as species with high carbon stock and low BVOCs emissions in Beijing,offering insights for future urban forest planning and eco-friendly urban environment development strategies.
基金Supported by National Natural Science Foundation of China,No.82305376,No.82374577,No.82305375,No.82074532,and No.82405567The Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD).
文摘BACKGROUND Electroacupuncture(EA)has been recognized for its beneficial effects on glucolipid metabolism,potentially through the regulation of sensory nerve coordination.The expandability of peripancreatic adipose tissue(PAT)is implicated in the transition from obesity to type 2 diabetes mellitus(T2DM).However,the specific pancreatic responses to EA require further elucidation.AIM To investigate the influence of EA on pancreatic glucolipid reduction level in a high-fat diet(HFD)rat model.METHODS To delineate the precise pathway through which EA mediates interactions bet ween PAT and islets,we assessed the expression levels of NGF,TRPV1,insulin,as well as other proteins in the pancreas and PAT.This approach enabled us to identify the acupoints that are most conducive to optimizing glycolipid metabolism.RESULTS The ST25,LI11 and ST37 groups attenuated HFD-induced obesity and insulin resistance(IR)to distinct degrees,with ST25 group having the greatest effect.EA at ST25 was found to modify the local regulatory influence of PAT on the pancreatic intrinsic nervous system.Specifically,EA at ST25 obviously activated the TRPV1-CGRP-islet beta cell pathway,contributing to the relief of glucolipid metabolic stress.The beneficial effects were abrogated following the chemical silencing of TRPV1 sensory afferents,confirming their indispensable role in EA-mediated regulation of islet and PAT function.Furthermore,in TRPV1 knockout mice,a reduction in PAT inflammation was observed,along with the recovery of islet beta cell function.EA at LI11 and ST37 demonstrated anti-inflammatory properties and helped ameliorate IR.CONCLUSION The PAT ecological niche influenced the progression from obesity to T2DM through various immunometabolic pathways.EA at ST25 could regulate glucolipid metabolism via the TRPV1-CGRP-islet beta cell pathway.
基金funded by the National Natural Science Foundation of China(Grant Nos.82220108002 to F.C.,82273737 to R.Z.,82473728 to Y.W.)the US National Institutes of Health(Grant Nos.CA209414,HL060710,ES000002 to D.C.C.,CA209414,CA249096 to Y.L.)the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD).R.Z.was partially supported by the Qing Lan Project of the Higher Education Institutions of Jiangsu Province and the Outstanding Young-Level Academic Leadership Training Program of Nanjing Medical University.
文摘Emerging evidence highlights the role of thyroid hormones in cancer,although findings are controversial.Research on thyroid-related traits in lung carcinogenesis is limited.Using UK Biobank data,we performed bidirectional Mendelian randomization(MR)to assess causal associations between lung cancer risk and thyroid dysfunction(hypothyroidism and hyperthyroidism)or functional traits(free thyroxine[FT4]and normal-range thyroid-stimulating hormone[TSH]).Furthermore,in the smoking-behavior-stratified MR analysis,we evaluated the mediating effect of thyroid-related phenotypes on the association between smoking behaviors and lung cancer.We demonstrated significant associations between lung cancer risk and hypothyroidism(hazard ratio[HR]=1.14,95%confidence interval[CI]=1.03–1.26,P=0.009)and hyperthyroidism(HR=1.55,95%CI=1.29–1.87,P=1.90×10^(-6))in the UKB.Moreover,the MR analysis indicated a causal effect of thyroid dysfunction on lung cancer risk(ORinverse variance weighted[IVW]=1.09,95%CI=1.05–1.13,P=3.12×10^(-6)for hypothyroidism;ORIVW=1.08,95%CI=1.04–1.12,P=8.14×10^(-5)for hyperthyroidism).We found that FT4 levels were protective against lung cancer risk(ORIVW=0.93,95%CI=0.87–0.99,P=0.030).Additionally,the stratified MR analysis demonstrated distinct causal effects of thyroid dysfunction on lung cancer risk among smokers.Hyperthyroidism mediated the effect of smoking behaviors,especially the age of smoking initiation(17.66%mediated),on lung cancer risk.Thus,thyroid dysfunction phenotypes play causal roles in lung cancer development exclusively among smokers and act as mediators in the causal pathway from smoking to lung cancer.
基金financially supported by the National Key Research and Development Program(Nos.2022YFB2502104 and 2022YFA1602700)the Key Research and Development Program of Jiangsu Provincial Department of Science and Technology of China(No.BE2022332)+4 种基金the Jiangsu Carbon Peak Carbon Neutralization Science and Technology Innovation Special Fund(No.BE2022605)the National Natural Science Foundation of China(Nos.22109073,22379071)the DECRA program of Australian Research Council(No.DE230100357)the JSPS KAKENHI(No.JP23K13703)the Center for Computational Materials Science,Institute for Materials Research,Tohoku University for the use of MASAMUNE-IMR(202312-SCKXX-0203)。
文摘Perovskite oxides(ABO_(3))are thought to be promising electrocatalysts for oxygen evolution reaction(OER),but their specific surface area(SSA)is too low(usually<10 m^(2) g^(−1)).Developing advanced ABO_(3) electrocatalysts with high SSA and optimized structure is of great significance but remains a tremendous challenge.Herein,we propose a general strategy for fabrication of mesoporous perovskite oxide nanosheets(MPONs)with controllable atomic doping via self-sacrificial template-induced nanostructure modulation.A variety of MPONs including LaFeO_(3),A-site-doped LaFeO_(3)(A-LaFeO_(3),where A is Pr,Nd,Sm,Eu,or Gd)and B-site-doped LaFeO_(3)(B-LaFeO_(3),where B is Mn,Co,Ni,Cu,or Zn)have been achieved.Interestingly,it is discovered that the catalytic activities of A-LaFeO_(3) MPONs as OER catalysts are overall higher than those of B-LaFeO_(3) ones.Especially,the screened Eu-LaFeO_(3) MPONs only require a low overpotential of 267 mV at 10 mA cm^(−2),outperforming most reported perovskite oxides.The superior catalytic activity of Eu-LaFeO_(3) MPONs is attributed to their favorable porous structure,which increases the density of active sites,and enhanced lattice oxygen participation,which improves the intrinsic activity.This study provides guidance for the design and controlled synthesis of advanced rare-earth-doped MPONs with ultrahigh SSA for enhanced electrocatalysis.
基金supported by National Natural Science Youth Foundation of China(No.22308294)National Natural Science Foundation of China(No.22179077)+1 种基金Postgraduate Research&Practice Innovation Program of Jiangsu Province(No.SJCX23_1868)Qing Lan Project of Jiangsu University and the Funding for school-level research projects of Yancheng Institute of Technology.
文摘Mn-based P2-type oxides are considered as promising cathodes for Na-ion batteries;however,they face significant challenges,including structural degradation when charged at high cutoff voltages and structural changes upon storing in a humid atmosphere.In response to these issues,we have designed an oxide with co-doping of Cu and Al which can balance both cost and structural stability.The redox reaction of Cu^(2+/3+)can provide certain charge compensation,and the introduction of Al can further suppress the Jahn-Teller effect of Mn,thereby achieving superior long-term cycling performance.The ex-situ XRD testing indicates that Cu/Al co-doping can effectively suppress the phase transition of P2-O2 at high voltage,thereby explaining the improvement in electrochemical performance.DFT calculations reveal a high chemical tolerance to moisture,with lower adsorption energy for H_(2)O compared to pure Na_(0.67)Cu_(0.25)Mn_(0.75)O_(2).A representative Na_(0.67)Cu_(0.20)Al_(0.05)Mn_(0.75)O_(2)cathode demonstrates impressive reversible capacities of 148.7 mAh/g at 0.2 C,along with a remarkable capacity retention of 79.1%(2 C,500 cycles).
基金Supported by the Shanghai Municipal Health Commission,China,No.GWV-10.2-YQ46.
文摘BACKGROUND Although extensive research has investigated attentional biases based on the looming vulnerability model of anxiety,the characteristics of attentional biases in individuals with looming cognitive styles(LCS)remain incompletely elucidated.No prior eye-tracking studies have examined the spatiotemporal dynamics of their threat-related attentional preferences.AIM To investigate the nature and temporal pattern of attentional biases toward threat stimuli in individuals exhibiting different levels of LCS using eye-tracking technology.METHODS A total of 212 participants were stratified according to their Looming Maladaptive Style Questionnaire scores.From the high and low scoring subgroups,35 participants were randomly selected for an eye-tracking experiment using a classic dot-probe paradigm featuring threat and neutral images.Four eye-tracking metrics,including first fixation latency,first fixation duration,total fixation duration,and fixation count,were analyzed to assess detection speed,attentional orienting,initial maintenance/avoidance,and overall engagement.RESULTS Distinct attentional bias patterns were observed between high and low LCS groups.High LCS individuals exhibited a vigilance-avoidance pattern characterized by initial vigilance toward threat stimuli(evidenced by faster detection and preferential orienting),followed by attentional avoidance,alongside sustained attention maintenance to threat.CONCLUSION These findings reveal a temporal dissociation between early vigilance and later avoidance during threat processing in high LCS individuals,providing novel empirical evidence to refine models of cognitive vulnerability and attentional dynamics in threat perception.
基金National Natural Science Foundation of China,No.82074532,No.82374577,No.82305375,No.82305376,and No.82405567The Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD).
文摘BACKGROUND Peripheral neuropathy caused by diabetes is closely related to the vicious cycle of oxidative stress and mitochondrial dysfunction resulting from metabolic abnormalities.The effects mediated by the silent information regulator type 2 homolog-1(SIRT1)/peroxisome proliferator-activated receptor-gamma coactivator-1α(PGC-1α)axis present new opportunities for the treatment of type 2 diabetic peripheral neuropathy(T2DPN),potentially breaking this harmful cycle.AIM To validate the effectiveness of electroacupuncture(EA)in the treatment of T2DPN and investigate its potential mechanism based on the SIRT1/PGC-1αaxis.METHODS The effects of EA were evaluated through assessments of metabolic changes,morphological observations,and functional examinations of the sciatic nerve,along with measurements of inflammation and oxidative stress.Proteins related to the SIRT1/PGC-1αaxis,involved in the regulation of mitochondrial biogenesis and antioxidative stress,were detected in the sciatic nerve using Western blotting to explain the underlying mechanism.A counterevidence group was created by injecting a SIRT1 inhibitor during EA intervention to support the hypothesis.RESULTS In addition to diabetes-related metabolic changes,T2DPN rats showed significant reductions in pain threshold after 9 weeks,suggesting abnormal peripheral nerve function.EA treatment partially restored metabolic control and reduced nerve damage in T2DPN rats.The SIRT1/PGC-1αaxis,which was downregulated in the model group,was upregulated by EA intervention.The endogenous antioxidant system related to the SIRT1/PGC-1αaxis,previously inhibited in diabetic rats,was reactivated.A similar trend was observed in inflammatory markers.When SIRT1 was inhibited in diabetic rats,these beneficial effects were abolished.CONCLUSION EA can alleviate the symptoms of T2DNP in experimental rats,and its effects may be related to the mitochondrial biogenesis and endogenous antioxidant system mediated by the SIRT1/PGC-1αaxis.
