Self-management interventions for chronic obstructive pulmonary disease(COPD)patients using mobile health technology are beneficial for relieving disease symptoms,improving patients’adherence to rehabilitation self-m...Self-management interventions for chronic obstructive pulmonary disease(COPD)patients using mobile health technology are beneficial for relieving disease symptoms,improving patients’adherence to rehabilitation self-management,and improving quality of life.This paper reviews the application of mobile health technology in self-management of patients with chronic obstructive pulmonary disease,introduces the application form of mobile health technology in self-management of patients with chronic obstructive pulmonary disease,summarizes its application effect in self-management of patients with chronic obstructive pulmonary disease,analyzes the problems and proposes solutions in the process of research and implementation at this stage,with a view to providing a theory for the application of mobile health technology in pulmonary rehabilitation and management of patients with chronic obstructive pulmonary disease This study summarize the effect of its application in the self-management of patients with chronic obstructive pulmonary disease.展开更多
Nonalcoholic fatty liver disease (NAFLD) is considered a major health epidemic with an estimated 32.4% worldwide prevalence. No drugs have yet been approved and therapeutic nodes remain a major unmet need. Long noncod...Nonalcoholic fatty liver disease (NAFLD) is considered a major health epidemic with an estimated 32.4% worldwide prevalence. No drugs have yet been approved and therapeutic nodes remain a major unmet need. Long noncoding RNAs are emerging as an important class of novel regulators influencing multiple biological processes and the pathogenesis of NAFLD. Herein, we described a novel long noncoding RNA, lnc_217, which was liver enriched and upregulated in high-fat diet-fed mice, and a genetic animal model of NAFLD. We found that liver specific knockdown of lnc_217 was resistant to high-fat diet-induced hepatic lipid accumulation and decreased serum lipid in mice. Mechanistically, we demonstrated that knockdown of lnc_217 not only decreased de novo lipogenesis by inhibiting sterol regulatory element binding protein-1c cleavage but also increased fatty acid β- oxidation through activation of peroxisome proliferator-activated receptor α and carnitine palmitoyltransferase-1α. Taken together, we conclude that lnc_217 may be a novel regulator of hepatic lipid metabolism and a potential therapeutic target for the treatment of hepatic steatosis and NAFLD-related metabolic disorders.展开更多
This paper reviews the concepts of emotional labor,organizational commitment,and work engagement among nurses.By integrating motivation theory,social exchange theory,and the job demands-resources model,it elaborates o...This paper reviews the concepts of emotional labor,organizational commitment,and work engagement among nurses.By integrating motivation theory,social exchange theory,and the job demands-resources model,it elaborates on the current research status and theoretical support for the interrelationships between these concepts in pairs.Through these theoretical frameworks,the paper explores potential causal relationships between emotional labor,organizational commitment,and work engagement,proposing that organizational commitment may mediate the relationship between emotional labor and work engagement.This study provides insights into the complex dynamics of emotional management,organizational identification,and work engagement in nursing,laying a foundation for further research in this domain.展开更多
Through the combing of relevant literature and the analysis of relevant psychological students’cases,the composition of family factors affecting college students’mental health is analyzed,the specific manifestations...Through the combing of relevant literature and the analysis of relevant psychological students’cases,the composition of family factors affecting college students’mental health is analyzed,the specific manifestations of college students’psychological problems are summarized,and the influence of family factors on college students’mental health status is analyzed in detail.The article finally puts forward suggestions and recommendations for strengthening college students’family education and tries to explore how to promote college students’mental health through improving family education.展开更多
Background and Aims:Hepatocellular carcinoma(HCC)remains challenging to treat in advanced stages,primarily due to the development of resistance to sorafenib.There is an urgent need for novel therapeutic strategies to ...Background and Aims:Hepatocellular carcinoma(HCC)remains challenging to treat in advanced stages,primarily due to the development of resistance to sorafenib.There is an urgent need for novel therapeutic strategies to overcome this resistance.This study aimed to investigate the potential of oleanolic acid(OA),a natural hepatoprotective compound,in mitigating sorafenib resistance and elucidate its underlying molecular mechanisms.Methods:Sorafenib-resistant Huh7 and HepG2 cell lines were established to mimic the resistant phenotype.The effects of OA on these cells were evaluated by assessing cell invasion,migration,and sensitivity to sorafenib.Gene expression analysis was conducted to identify molecular changes induced by OA treatment,with a focus on fabp3 expression.Results:Oleanolic acid significantly inhibited the invasive and migratory capabilities of sorafenib-resistant Huh7 and HepG2 cells(p<0.01).Furthermore,OA treatment downregulated fabp3 expression and restored the cells’sensitivity to sorafenib.Conclusions:Oleanolic acid shows promise as an adjunct therapy for overcoming sorafenib resistance in HCC.By reducing cell aggressiveness and restoring drug sensitivity,OA may enhance the therapeutic efficacy of current treatments for advanced HCC.展开更多
Coordinated gene transcription in plastids and nuclei is necessary for the photosynthetic apparatus assembly during chloroplast biogenesis. Despite the identification of several transcription factors regulating nuclea...Coordinated gene transcription in plastids and nuclei is necessary for the photosynthetic apparatus assembly during chloroplast biogenesis. Despite the identification of several transcription factors regulating nuclear-encoded photosynthetic gene transcription, transcription factors regulating plastid gene transcription are barely reported. Here, we report that BAI1 (“albino” in Chinese), a nucleus–plastid dual-targeted C2H2-type zinc-finger transcription factor in Arabidopsis, positively regulates and tunes the transcription of both nuclear and plastid genes. Knockout of BAI1 blocks chloroplast formation, producing albino seedlings and lethality. In plastids, BAI1 is a newly identified functional component of the pTAC (transcriptionally active chromosome complex), which interacts with another pTAC component, pTAC12/PAP5/HMR, to allow the effective assembly of plastid-encoded RNA polymerase (PEP) complexes. The transcript levels of PEP-dependent genes were reduced in the bai1 mutant. In contrast, the accumulation of nuclear-encoded RNA polymerase (NEP)-dependent transcripts was increased, suggesting that BAI1 is critical in maintaining PEP activity. BAI1 directly binds to the promoter regions of nuclear genes RbcSs and a plastid gene RbcL to activate their expression for RubisCO assembly. AtBAI1 homologs TaBAI1, GmBAI1a, and GmBAI1b from monocots and dicots can fully complement the defects of the Arabidopsis bai1 mutant. In contrast, Physcomitrium patens BAI1 (PpBAI1) only partially complements the bai1 mutant. Phylogenetic analysis of BAI1 and HMR uncovered that both components arose from late-diverging streptophyte algae, following a conserved evolutionary path during terrestrialization. In summary, this work unveils a BAI1-mediated transcription regulatory mechanism synchronizing the transcription of nuclear and plastid genes, necessary for hybrid photosynthetic complex assembly. This could be an intrinsic feature facilitating plant terrestrialization.展开更多
Background: Gut-resident macrophages (gMacs) supplemented by monocytes-to-gMacs differentiation play a critical role in maintaining intestinal homeostasis. Activating transcription factor 4 (ATF4) is involved in immun...Background: Gut-resident macrophages (gMacs) supplemented by monocytes-to-gMacs differentiation play a critical role in maintaining intestinal homeostasis. Activating transcription factor 4 (ATF4) is involved in immune cell differentiation. We therefore set out to investigate the role of ATF4-regulated monocytes-to-gMacs differentiation in sepsis-induced intestinal injury.Methods: Sepsis was induced in C57BL/6 wild type (WT) mice andAtf4-knockdown (Atf4+/-) mice by cecal ligation and puncture or administration of lipopolysaccharide (LPS). Colon, peripheral blood mononuclear cells, sera, lung, liver, and mesenteric lymph nodes were collected for flow cytometry, hematoxylin and eosin staining, immunohistochemistry, quantitative reverse transcription polymerase chain reaction, and enzyme-linked immunosorbent assay, respectively.Results: CD64, CD11b, Ly6C, major histocompatibility complex-II (MHC-II), CX3CR1, Ly6G, and SSC were identified as optimal primary markers for detecting the process of monocytes-to-gMacs differentiation in the colon of WT mice. Monocytes-to-gMacs differentiation was impaired in the colon during sepsis and was associated with decreased expression of ATF4 in P1 (Ly6Chi monocytes), the precursor cells of gMacs.Atf4 knockdown exacerbated the impairment of monocytes-to-gMacs differentiation in response to LPS, resulting in a significant reduction of gMacs in the colon. Furthermore, compared with WT mice,Atf4+/- mice exhibited higher pathology scores, increased expression of inflammatory factor genes (TNF-α, IL-1β), suppressed expression of CD31 and vascular endothelial-cadherin in the colon, and increased translocation of intestinal bacteria to lymph nodes and lungs following exposure to LPS. However, the aggravation of sepsis-induced intestinal injury resulting fromAtf4 knockdown was not caused by the enhanced inflammatory effect of Ly6Chi monocytes and gMacs.Conclusion: ATF4, as a novel regulator of monocytes-to-gMacs differentiation, plays a critical role in protecting mice against sepsis-induced intestinal injury, suggesting that ATF4 might be a potential therapeutic target for sepsis treatment.展开更多
An electrochemical sensor for determination of hydroquinone （HQ） and catechol （CC） was developed using Au nanoparticles （AuNPs） fabricated on reduced graphene oxide/polyimide （PIIRGO） film by electroless depos...An electrochemical sensor for determination of hydroquinone （HQ） and catechol （CC） was developed using Au nanoparticles （AuNPs） fabricated on reduced graphene oxide/polyimide （PIIRGO） film by electroless deposition. The electrochemical behaviors of HQ and CC at PIIRGO-AuNPs electrode were investigated by cyclic voltammetry （CV） and differential pulse voltammetry （DPV）. Under the optimized condition, the current responses at PI/RGO-AuNPs electrode were linear over ranges from 1 to 654 mol/L for HQ and from 2 to 1289 mol/L for CC, with the detection limits of 0.09 and 0.2 mollL, respectively. The proposed electrode exhibited good reproducibility, stability and selectivity. In addition, the proposed electrode was successfully applied in the determination of HQ and CC in tap water and the Yellow River samples.展开更多
基金supported by the 2025 Hangzhou Normal University Teaching Development and Reform Project(Project No.JG2025320)。
文摘Self-management interventions for chronic obstructive pulmonary disease(COPD)patients using mobile health technology are beneficial for relieving disease symptoms,improving patients’adherence to rehabilitation self-management,and improving quality of life.This paper reviews the application of mobile health technology in self-management of patients with chronic obstructive pulmonary disease,introduces the application form of mobile health technology in self-management of patients with chronic obstructive pulmonary disease,summarizes its application effect in self-management of patients with chronic obstructive pulmonary disease,analyzes the problems and proposes solutions in the process of research and implementation at this stage,with a view to providing a theory for the application of mobile health technology in pulmonary rehabilitation and management of patients with chronic obstructive pulmonary disease This study summarize the effect of its application in the self-management of patients with chronic obstructive pulmonary disease.
基金This work was supported by grants from the National Natural Science Foundation of China(Grant Nos.32130050,32201064,and 82170838)the Natural Science Research Project of Universities in Jiangsu Province(Grant No.21KJB180003).
文摘Nonalcoholic fatty liver disease (NAFLD) is considered a major health epidemic with an estimated 32.4% worldwide prevalence. No drugs have yet been approved and therapeutic nodes remain a major unmet need. Long noncoding RNAs are emerging as an important class of novel regulators influencing multiple biological processes and the pathogenesis of NAFLD. Herein, we described a novel long noncoding RNA, lnc_217, which was liver enriched and upregulated in high-fat diet-fed mice, and a genetic animal model of NAFLD. We found that liver specific knockdown of lnc_217 was resistant to high-fat diet-induced hepatic lipid accumulation and decreased serum lipid in mice. Mechanistically, we demonstrated that knockdown of lnc_217 not only decreased de novo lipogenesis by inhibiting sterol regulatory element binding protein-1c cleavage but also increased fatty acid β- oxidation through activation of peroxisome proliferator-activated receptor α and carnitine palmitoyltransferase-1α. Taken together, we conclude that lnc_217 may be a novel regulator of hepatic lipid metabolism and a potential therapeutic target for the treatment of hepatic steatosis and NAFLD-related metabolic disorders.
文摘This paper reviews the concepts of emotional labor,organizational commitment,and work engagement among nurses.By integrating motivation theory,social exchange theory,and the job demands-resources model,it elaborates on the current research status and theoretical support for the interrelationships between these concepts in pairs.Through these theoretical frameworks,the paper explores potential causal relationships between emotional labor,organizational commitment,and work engagement,proposing that organizational commitment may mediate the relationship between emotional labor and work engagement.This study provides insights into the complex dynamics of emotional management,organizational identification,and work engagement in nursing,laying a foundation for further research in this domain.
文摘Through the combing of relevant literature and the analysis of relevant psychological students’cases,the composition of family factors affecting college students’mental health is analyzed,the specific manifestations of college students’psychological problems are summarized,and the influence of family factors on college students’mental health status is analyzed in detail.The article finally puts forward suggestions and recommendations for strengthening college students’family education and tries to explore how to promote college students’mental health through improving family education.
基金supported by the Horizontal Project of Jiangsu Medical College(2021010401)the collaborative innovation project for“Double First-Class”Discipline Heilongjiang Province(LJGXCG2023-089)+2 种基金the Joint Guidance Project of Natural Science Foundation of Heilongjiang Province(LH2022H090,PL2024H002)the Innovation Team of Heilongjiang Provincial Department of Education(2024-KYYWF-0617)the he East-Extreme Discipline Construction Team of Jiamusi University(DJXSTD202404).
文摘Background and Aims:Hepatocellular carcinoma(HCC)remains challenging to treat in advanced stages,primarily due to the development of resistance to sorafenib.There is an urgent need for novel therapeutic strategies to overcome this resistance.This study aimed to investigate the potential of oleanolic acid(OA),a natural hepatoprotective compound,in mitigating sorafenib resistance and elucidate its underlying molecular mechanisms.Methods:Sorafenib-resistant Huh7 and HepG2 cell lines were established to mimic the resistant phenotype.The effects of OA on these cells were evaluated by assessing cell invasion,migration,and sensitivity to sorafenib.Gene expression analysis was conducted to identify molecular changes induced by OA treatment,with a focus on fabp3 expression.Results:Oleanolic acid significantly inhibited the invasive and migratory capabilities of sorafenib-resistant Huh7 and HepG2 cells(p<0.01).Furthermore,OA treatment downregulated fabp3 expression and restored the cells’sensitivity to sorafenib.Conclusions:Oleanolic acid shows promise as an adjunct therapy for overcoming sorafenib resistance in HCC.By reducing cell aggressiveness and restoring drug sensitivity,OA may enhance the therapeutic efficacy of current treatments for advanced HCC.
基金supported by the National Natural Science Foundation of China National Natural Science Foundation of China(32070550 and 31872631).
文摘Coordinated gene transcription in plastids and nuclei is necessary for the photosynthetic apparatus assembly during chloroplast biogenesis. Despite the identification of several transcription factors regulating nuclear-encoded photosynthetic gene transcription, transcription factors regulating plastid gene transcription are barely reported. Here, we report that BAI1 (“albino” in Chinese), a nucleus–plastid dual-targeted C2H2-type zinc-finger transcription factor in Arabidopsis, positively regulates and tunes the transcription of both nuclear and plastid genes. Knockout of BAI1 blocks chloroplast formation, producing albino seedlings and lethality. In plastids, BAI1 is a newly identified functional component of the pTAC (transcriptionally active chromosome complex), which interacts with another pTAC component, pTAC12/PAP5/HMR, to allow the effective assembly of plastid-encoded RNA polymerase (PEP) complexes. The transcript levels of PEP-dependent genes were reduced in the bai1 mutant. In contrast, the accumulation of nuclear-encoded RNA polymerase (NEP)-dependent transcripts was increased, suggesting that BAI1 is critical in maintaining PEP activity. BAI1 directly binds to the promoter regions of nuclear genes RbcSs and a plastid gene RbcL to activate their expression for RubisCO assembly. AtBAI1 homologs TaBAI1, GmBAI1a, and GmBAI1b from monocots and dicots can fully complement the defects of the Arabidopsis bai1 mutant. In contrast, Physcomitrium patens BAI1 (PpBAI1) only partially complements the bai1 mutant. Phylogenetic analysis of BAI1 and HMR uncovered that both components arose from late-diverging streptophyte algae, following a conserved evolutionary path during terrestrialization. In summary, this work unveils a BAI1-mediated transcription regulatory mechanism synchronizing the transcription of nuclear and plastid genes, necessary for hybrid photosynthetic complex assembly. This could be an intrinsic feature facilitating plant terrestrialization.
基金National Natural Science Foundation of China(Nos. 82172152, 81873944, 82172154, 81971869, 82171729)Natural Science Foundation of Shanghai(No.19ZR1442500)Research Fund of Ruijin Hospital North Affiliated to Shanghai Jiao Tong University School of Medicine(No.2020ZY11)
文摘Background: Gut-resident macrophages (gMacs) supplemented by monocytes-to-gMacs differentiation play a critical role in maintaining intestinal homeostasis. Activating transcription factor 4 (ATF4) is involved in immune cell differentiation. We therefore set out to investigate the role of ATF4-regulated monocytes-to-gMacs differentiation in sepsis-induced intestinal injury.Methods: Sepsis was induced in C57BL/6 wild type (WT) mice andAtf4-knockdown (Atf4+/-) mice by cecal ligation and puncture or administration of lipopolysaccharide (LPS). Colon, peripheral blood mononuclear cells, sera, lung, liver, and mesenteric lymph nodes were collected for flow cytometry, hematoxylin and eosin staining, immunohistochemistry, quantitative reverse transcription polymerase chain reaction, and enzyme-linked immunosorbent assay, respectively.Results: CD64, CD11b, Ly6C, major histocompatibility complex-II (MHC-II), CX3CR1, Ly6G, and SSC were identified as optimal primary markers for detecting the process of monocytes-to-gMacs differentiation in the colon of WT mice. Monocytes-to-gMacs differentiation was impaired in the colon during sepsis and was associated with decreased expression of ATF4 in P1 (Ly6Chi monocytes), the precursor cells of gMacs.Atf4 knockdown exacerbated the impairment of monocytes-to-gMacs differentiation in response to LPS, resulting in a significant reduction of gMacs in the colon. Furthermore, compared with WT mice,Atf4+/- mice exhibited higher pathology scores, increased expression of inflammatory factor genes (TNF-α, IL-1β), suppressed expression of CD31 and vascular endothelial-cadherin in the colon, and increased translocation of intestinal bacteria to lymph nodes and lungs following exposure to LPS. However, the aggravation of sepsis-induced intestinal injury resulting fromAtf4 knockdown was not caused by the enhanced inflammatory effect of Ly6Chi monocytes and gMacs.Conclusion: ATF4, as a novel regulator of monocytes-to-gMacs differentiation, plays a critical role in protecting mice against sepsis-induced intestinal injury, suggesting that ATF4 might be a potential therapeutic target for sepsis treatment.
基金Acknowledgement This work was supported by the National Natural Science Foundation of China （Grant No. 51372106）.
文摘An electrochemical sensor for determination of hydroquinone （HQ） and catechol （CC） was developed using Au nanoparticles （AuNPs） fabricated on reduced graphene oxide/polyimide （PIIRGO） film by electroless deposition. The electrochemical behaviors of HQ and CC at PIIRGO-AuNPs electrode were investigated by cyclic voltammetry （CV） and differential pulse voltammetry （DPV）. Under the optimized condition, the current responses at PI/RGO-AuNPs electrode were linear over ranges from 1 to 654 mol/L for HQ and from 2 to 1289 mol/L for CC, with the detection limits of 0.09 and 0.2 mollL, respectively. The proposed electrode exhibited good reproducibility, stability and selectivity. In addition, the proposed electrode was successfully applied in the determination of HQ and CC in tap water and the Yellow River samples.
