17β-Trenbolone(17-TB)is well documented as an environmental endocrine disruptor in aquatic biological studies,but its effects on mammals remain poorly understood.Furthermore,17-TB acts as a hormone with properties si...17β-Trenbolone(17-TB)is well documented as an environmental endocrine disruptor in aquatic biological studies,but its effects on mammals remain poorly understood.Furthermore,17-TB acts as a hormone with properties similar to testosterone,and the consequences of juvenile exposure on adult social behavior remain uncertain.Bisphenol A(BPA)acts as an estrogen-like hormone,compared to 17-TB.Three-week-old male Balb/c mice were exposed orally to 17-TB(100μg/(kg·day))and BPA(4 mg/(kg·day))for 28 days.Assessments of social interactions and a three-chamber test showed that 17-TB increased virility in male mice,intensified both male and female sexual behavior,and attracted and accepted female mice.It also increased social dominance through tube tests inmalemice and markedly activated the c-Fos^(+)immune response in themedial prefrontal cortex(mPFC)and basal amygdala(BLA).ELISA data showed that 17-TB and BPA exposure significantly affected serum gonadotropin-releasing hormone(GnRH),growth hormone(GH),estradiol(E2),and luteinizing hormone(LH)levels,as well as testicular lesions and androgen receptor(ARβ)and estrogen receptor(ERα)synthesis.Testicular transcriptomic analysis further confirmed that could disrupt steroid synthesis and linoleic acid-related biometabolic processes.These findings suggest the influence of 17-TB and BPA exposure on sexual behavior and fertility in male mice,possibly through modulation of the hypothalamic-pituitary-gonadal axis.This study provides insights relevant to human reproductive health and neuro-social behavioral research,and the potential risk of environmental disturbances should not be overlooked.展开更多
Insulin resistance is a key pathological process in the development of type 2 diabetes,which can also lead to depression,anxiety and other symptoms.With the accelerated pace of life,food with high-energy and additives...Insulin resistance is a key pathological process in the development of type 2 diabetes,which can also lead to depression,anxiety and other symptoms.With the accelerated pace of life,food with high-energy and additives is becoming more and more common in people's diet.Propionate is a naturally existing short-chain fatty acid salt widely used as a food preservative,but studies have found that low concentrations of propionate can induce insulin resistance.Theaflavins(TFs)are characteristic components of black tea,which have hypoglycemic and lipid-lowering effects.In the present study,propionate combined with high-fat diet was used to induce insulin resistance in mice and treated them with TFs.The results showed that propionate could aggravate insulin resistance and anxiety induced by high-fat diet in mice.TFs alleviated insulin resistance and anxiety induced by propionate combined with high-fat diet by improving the disorder of metabolic gene expression and inflammation in the adipose and liver tissue,as well as neuroinflammation in the brain.Our study suggests that the food additive propionate combined with a high-fat diet may be harmful to organismal health.As a natural drug,TFs have the potential to alleviate insulin resistance induced by propionate combined with high-fat diet.展开更多
Increasing evidence indicates that disturbance of the clock genes,which leads to systemic endocrine perturbation,plays a crucial role in the pathogenesis of metabolic and liver diseases.Fluorene-9-bisphenol(BHPF)is ut...Increasing evidence indicates that disturbance of the clock genes,which leads to systemic endocrine perturbation,plays a crucial role in the pathogenesis of metabolic and liver diseases.Fluorene-9-bisphenol(BHPF)is utilized in the manufacturing of plastic materials but its biological effects on liver homeostasis remain unknown.The impacts and involved mechanisms of BHPF on the liver diseases,metabolism,and circadian clock were comprehensively studied by zebrafish andmouse models.The therapeutic effect of melatonin(MT)was also verified.Zebrafish and mousemodels with either acute exposure(0.5 and 1μmol/L,1-4 days post-fertilization)or chronic oral exposure(0.5 and 50 mg/(kg·2 days),30 days)were established with various BHPF concentrations.Herein,we identified a crucial role for estrogenic regulation in liver development and circadian locomotor rhythms damaged by BHPF in a zebrafish model.BHPF mice showed chaos in circadian activity through the imbalance of circadian clock component Brain and Muscle Aryl hydrocarbon receptor nuclear translocator-like 1 in the liver and brain.The liver sexual dimorphic alteration alongwith reduced growth hormone and estrogens played a critical role in damaged glucose metabolism,hepatic inflammation,and fibrosis induced by BHPF.Besides,sleep improvement by exogenous MT alleviated BHPF-related glucose metabolism and liver injury in mice.We proposed the pathogenesis of metabolic and liver disease resulting from BHPF and promising targeted therapy for livermetabolism disorders associated with endocrine perturbation chemicals.These results might play a warning role in the administration of endocrine-disrupting chemicals in everyday life and various industry applications.展开更多
基金supported by the National Natural Science Foundation of China(Nos.62027812 and 62273186).
文摘17β-Trenbolone(17-TB)is well documented as an environmental endocrine disruptor in aquatic biological studies,but its effects on mammals remain poorly understood.Furthermore,17-TB acts as a hormone with properties similar to testosterone,and the consequences of juvenile exposure on adult social behavior remain uncertain.Bisphenol A(BPA)acts as an estrogen-like hormone,compared to 17-TB.Three-week-old male Balb/c mice were exposed orally to 17-TB(100μg/(kg·day))and BPA(4 mg/(kg·day))for 28 days.Assessments of social interactions and a three-chamber test showed that 17-TB increased virility in male mice,intensified both male and female sexual behavior,and attracted and accepted female mice.It also increased social dominance through tube tests inmalemice and markedly activated the c-Fos^(+)immune response in themedial prefrontal cortex(mPFC)and basal amygdala(BLA).ELISA data showed that 17-TB and BPA exposure significantly affected serum gonadotropin-releasing hormone(GnRH),growth hormone(GH),estradiol(E2),and luteinizing hormone(LH)levels,as well as testicular lesions and androgen receptor(ARβ)and estrogen receptor(ERα)synthesis.Testicular transcriptomic analysis further confirmed that could disrupt steroid synthesis and linoleic acid-related biometabolic processes.These findings suggest the influence of 17-TB and BPA exposure on sexual behavior and fertility in male mice,possibly through modulation of the hypothalamic-pituitary-gonadal axis.This study provides insights relevant to human reproductive health and neuro-social behavioral research,and the potential risk of environmental disturbances should not be overlooked.
基金jointly supported by Special Fund for Basic Research on Scientific Instruments of the Chinese National Natural Science Foundation(62027812)National Innovation and Entrepreneurship Project for College Students(202210055092)。
文摘Insulin resistance is a key pathological process in the development of type 2 diabetes,which can also lead to depression,anxiety and other symptoms.With the accelerated pace of life,food with high-energy and additives is becoming more and more common in people's diet.Propionate is a naturally existing short-chain fatty acid salt widely used as a food preservative,but studies have found that low concentrations of propionate can induce insulin resistance.Theaflavins(TFs)are characteristic components of black tea,which have hypoglycemic and lipid-lowering effects.In the present study,propionate combined with high-fat diet was used to induce insulin resistance in mice and treated them with TFs.The results showed that propionate could aggravate insulin resistance and anxiety induced by high-fat diet in mice.TFs alleviated insulin resistance and anxiety induced by propionate combined with high-fat diet by improving the disorder of metabolic gene expression and inflammation in the adipose and liver tissue,as well as neuroinflammation in the brain.Our study suggests that the food additive propionate combined with a high-fat diet may be harmful to organismal health.As a natural drug,TFs have the potential to alleviate insulin resistance induced by propionate combined with high-fat diet.
基金supported by the National Natural Science Foundation of China(No.62027812)Tianjin Key Medical Discipline(Specialty)Construction Project(No.TJSYXZDXK006)Tianjin Municipal Bureau of Public Health(No.RC20037).
文摘Increasing evidence indicates that disturbance of the clock genes,which leads to systemic endocrine perturbation,plays a crucial role in the pathogenesis of metabolic and liver diseases.Fluorene-9-bisphenol(BHPF)is utilized in the manufacturing of plastic materials but its biological effects on liver homeostasis remain unknown.The impacts and involved mechanisms of BHPF on the liver diseases,metabolism,and circadian clock were comprehensively studied by zebrafish andmouse models.The therapeutic effect of melatonin(MT)was also verified.Zebrafish and mousemodels with either acute exposure(0.5 and 1μmol/L,1-4 days post-fertilization)or chronic oral exposure(0.5 and 50 mg/(kg·2 days),30 days)were established with various BHPF concentrations.Herein,we identified a crucial role for estrogenic regulation in liver development and circadian locomotor rhythms damaged by BHPF in a zebrafish model.BHPF mice showed chaos in circadian activity through the imbalance of circadian clock component Brain and Muscle Aryl hydrocarbon receptor nuclear translocator-like 1 in the liver and brain.The liver sexual dimorphic alteration alongwith reduced growth hormone and estrogens played a critical role in damaged glucose metabolism,hepatic inflammation,and fibrosis induced by BHPF.Besides,sleep improvement by exogenous MT alleviated BHPF-related glucose metabolism and liver injury in mice.We proposed the pathogenesis of metabolic and liver disease resulting from BHPF and promising targeted therapy for livermetabolism disorders associated with endocrine perturbation chemicals.These results might play a warning role in the administration of endocrine-disrupting chemicals in everyday life and various industry applications.
