Dear Editors,Bispecific antibodies(bsAbs),which possess the unique ability to bind to two different antigens simultaneously(Labrijn et al.,2019),have emerged as powerful tools in the field of cancer immunotherapy and ...Dear Editors,Bispecific antibodies(bsAbs),which possess the unique ability to bind to two different antigens simultaneously(Labrijn et al.,2019),have emerged as powerful tools in the field of cancer immunotherapy and pathogen prevention.Their design and production are of great significance,as they can enhance the effectiveness of targeted therapy by improving the accuracy and efficiency of attacks against multiple targets.However,compared with traditional monoclonal antibodies,the design and production of bsAbs are more complex.One major challenge facing the conventional IgG bispecific format is the efficient pairing of different chains,known as the chain association issue.To overcome this,researchers have sought to promote heavychain heterodimerization through Fc engineering techniques,such as knobs-intoholes(KiH)and controlled Fab arm exchange.Others have highlighted the benefit of simple heavy-light chain paired formats,as they completely bypass the chain association issue while offering improved manufacturability.展开更多
基金supported by the National Natural Science Foundation of China(81991491,32300777)the Fundamental Research Funds for the Central Universities(20720220006)+1 种基金China Postdoctoral Science Foundation(2022M722659)CAMS Innovation Fund for Medical Sciences(2019RU022).
文摘Dear Editors,Bispecific antibodies(bsAbs),which possess the unique ability to bind to two different antigens simultaneously(Labrijn et al.,2019),have emerged as powerful tools in the field of cancer immunotherapy and pathogen prevention.Their design and production are of great significance,as they can enhance the effectiveness of targeted therapy by improving the accuracy and efficiency of attacks against multiple targets.However,compared with traditional monoclonal antibodies,the design and production of bsAbs are more complex.One major challenge facing the conventional IgG bispecific format is the efficient pairing of different chains,known as the chain association issue.To overcome this,researchers have sought to promote heavychain heterodimerization through Fc engineering techniques,such as knobs-intoholes(KiH)and controlled Fab arm exchange.Others have highlighted the benefit of simple heavy-light chain paired formats,as they completely bypass the chain association issue while offering improved manufacturability.
