Panax notoginseng-steamed chicken(PNSC)is a common medicinal diet in China.In this study,we conducted comprehensive analysis to determine the effect of PNSC on uterine involution in postpartum mice and human umbilical...Panax notoginseng-steamed chicken(PNSC)is a common medicinal diet in China.In this study,we conducted comprehensive analysis to determine the effect of PNSC on uterine involution in postpartum mice and human umbilical vein endothelial cells.The role of the phosphatidylinositide 3-kinases(PI3K)/protein kinase B(AKT)/mammalian target of rapamycin(m TOR)pathway in this process was explored.Results showed that PNSC promoted the recovery of endometrial hyperplasia and uterine index in postpartum mice.In vitro and in vivo experiments indicated that PNSC activated PI3K/AKT/m TOR signaling pathway,promoted the proliferation of human umbilical vein endothelial cells(HUVEC),up-regulated the expression of vascular endothelial growth factor(VEGF),and its binding to vascular endothelial growth factor receptor 2(VEGFR2).Moreover,it increased the expression of PCNA,MMP9,and Cyclin D1 in the nucleus.It can also up-regulated the secretion of hormones,such as prolactin(PRL),progesterone(P),as well as the level of VEGF in mice,and down-regulated the secretion of endothelin-1 hormones(ET-1),thereby promoting uterine involution.In conclusion,this study demonstrates that PNSC can regulate angiogenesis to promote uterine involution by activating the PI3K/AKT/mTOR pathway.展开更多
Panax notoginseng (Burk) F.H. Chen is one of the most famous Chinese traditional medicinal plants, which belongs to Panax genus under Araliaceae family. At the present, Panax notoginseng cultivated is a mix colony. Us...Panax notoginseng (Burk) F.H. Chen is one of the most famous Chinese traditional medicinal plants, which belongs to Panax genus under Araliaceae family. At the present, Panax notoginseng cultivated is a mix colony. Using DALP makers, this article studied on genetic diversity of cultivated populations of Panax notoginseng in Wenshan County of Yunnan province and Napo country of Guangxi province. And the results showed that there were 260 polymorphic loci detected from the total of 292 in 13 populations, and there was great part of genetic diversity found between populations and the genetic differentiations were lower within populations. So there is broad prospect in good species breeding. And it can provide basic information for resource protection and sustainable use of Panax notoginseng.展开更多
Panax notoginseng saponins(PNS)are the main active components of Panax notoginseng.But after oral administration,they need to be converted into rare ginsenosides by human gut microbiota and gastric juice before they c...Panax notoginseng saponins(PNS)are the main active components of Panax notoginseng.But after oral administration,they need to be converted into rare ginsenosides by human gut microbiota and gastric juice before they can be readily absorbed into the bloodstream and exert their effects.The sources of rare ginsenosides are extremely limited in P.notoginseng and other medical plants,which hinders their application in functional foods and drugs.Therefore,the production of rare ginsenosides by the transformation of PNS using Aspergillus fumigatus was studied in this research.During 50 days at 25℃and 150 rpm,A.fumigatus transformed PNS to 14 products(1-14).They were iso-lated by varied chromatographic methods,such as silica gel column chromatography,Rp-C18 reversed phase column chromatography,semi-preparative HPLC,Sephadex LH-20 gel column chromatography,and elucidated on the basis of their 1H-NMR,13C-NMR and ESIMS spectroscopic data.Then,the transformed products(1-14)were isolated and identified as Rk3,Rh4,20(R)-Rh1,20(S)-Protopanaxatriol,C-K,20(R)-Rg3,20(S)-Rg3,20(S)-Rg2,20(R)-R2,Rk1,Rg5,20(S)-R2,20(R)-Rg2,and 20(S)-I,respectively.In addition,all transformed products(1-14)were tested for their antimicrobial activity.Among them,compounds 5(C-K)and 7[20(S)-Rg3]showed moderate antimicrobial activities against Staphylococcus aureus and Candida albicans with MIC values of 6.25,1.25μg/mL and 1.25,25μg/mL,respectively.This study lays the foundation for production of rare ginsenosides.展开更多
Panax notoginseng,a perennial herb of the genus Panax in the family Araliaceae,has played an important role in clinical treatment in China for thousands of years because of its extensive pharmacological effects.Here,w...Panax notoginseng,a perennial herb of the genus Panax in the family Araliaceae,has played an important role in clinical treatment in China for thousands of years because of its extensive pharmacological effects.Here,we report a high-quality reference genome of P.notoginseng,with a genome size up to 2.66 Gb and a contig N50 of 1.12 Mb,produced with third-generation PacBio sequencing technology.This is the first chromosome-level genome assembly for the genus Panax.Through genome evolution analysis,we explored phylogenetic and whole-genome duplication events and examined their impact on saponin biosynthesis.We performed a detailed transcriptional analysis of P.notoginseng and explored genelevel mechanisms that regulate the formation of characteristic tubercles.Next,we studied the biosynthesis and regulation of saponins at temporal and spatial levels.We combined multi-omics data to identify genes that encode key enzymes in the P.notoginseng terpenoid biosynthetic pathway.Finally,we identified five glycosyltransferase genes whose products catalyzed the formation of different ginsenosides in P.notoginseng.The genetic information obtained in this study provides a resource for further exploration of the growth characteristics,cultivation,breeding,and saponin biosynthesis of P.notoginseng.展开更多
基金supported by National Key Research and Development Plan(2023YFC3503802)Scientific and Technological Innovation Project of China Academy of Chinese Medical Sciences(C12021B013)+1 种基金Yunnan Major Scientific and Technological Projects(202202AG050021)the National Key Research and Development Program(2021YFD1000201)。
文摘Panax notoginseng-steamed chicken(PNSC)is a common medicinal diet in China.In this study,we conducted comprehensive analysis to determine the effect of PNSC on uterine involution in postpartum mice and human umbilical vein endothelial cells.The role of the phosphatidylinositide 3-kinases(PI3K)/protein kinase B(AKT)/mammalian target of rapamycin(m TOR)pathway in this process was explored.Results showed that PNSC promoted the recovery of endometrial hyperplasia and uterine index in postpartum mice.In vitro and in vivo experiments indicated that PNSC activated PI3K/AKT/m TOR signaling pathway,promoted the proliferation of human umbilical vein endothelial cells(HUVEC),up-regulated the expression of vascular endothelial growth factor(VEGF),and its binding to vascular endothelial growth factor receptor 2(VEGFR2).Moreover,it increased the expression of PCNA,MMP9,and Cyclin D1 in the nucleus.It can also up-regulated the secretion of hormones,such as prolactin(PRL),progesterone(P),as well as the level of VEGF in mice,and down-regulated the secretion of endothelin-1 hormones(ET-1),thereby promoting uterine involution.In conclusion,this study demonstrates that PNSC can regulate angiogenesis to promote uterine involution by activating the PI3K/AKT/mTOR pathway.
文摘Panax notoginseng (Burk) F.H. Chen is one of the most famous Chinese traditional medicinal plants, which belongs to Panax genus under Araliaceae family. At the present, Panax notoginseng cultivated is a mix colony. Using DALP makers, this article studied on genetic diversity of cultivated populations of Panax notoginseng in Wenshan County of Yunnan province and Napo country of Guangxi province. And the results showed that there were 260 polymorphic loci detected from the total of 292 in 13 populations, and there was great part of genetic diversity found between populations and the genetic differentiations were lower within populations. So there is broad prospect in good species breeding. And it can provide basic information for resource protection and sustainable use of Panax notoginseng.
基金supported by the National Natural Science Foundation of China(Grant number:32060104).
文摘Panax notoginseng saponins(PNS)are the main active components of Panax notoginseng.But after oral administration,they need to be converted into rare ginsenosides by human gut microbiota and gastric juice before they can be readily absorbed into the bloodstream and exert their effects.The sources of rare ginsenosides are extremely limited in P.notoginseng and other medical plants,which hinders their application in functional foods and drugs.Therefore,the production of rare ginsenosides by the transformation of PNS using Aspergillus fumigatus was studied in this research.During 50 days at 25℃and 150 rpm,A.fumigatus transformed PNS to 14 products(1-14).They were iso-lated by varied chromatographic methods,such as silica gel column chromatography,Rp-C18 reversed phase column chromatography,semi-preparative HPLC,Sephadex LH-20 gel column chromatography,and elucidated on the basis of their 1H-NMR,13C-NMR and ESIMS spectroscopic data.Then,the transformed products(1-14)were isolated and identified as Rk3,Rh4,20(R)-Rh1,20(S)-Protopanaxatriol,C-K,20(R)-Rg3,20(S)-Rg3,20(S)-Rg2,20(R)-R2,Rk1,Rg5,20(S)-R2,20(R)-Rg2,and 20(S)-I,respectively.In addition,all transformed products(1-14)were tested for their antimicrobial activity.Among them,compounds 5(C-K)and 7[20(S)-Rg3]showed moderate antimicrobial activities against Staphylococcus aureus and Candida albicans with MIC values of 6.25,1.25μg/mL and 1.25,25μg/mL,respectively.This study lays the foundation for production of rare ginsenosides.
基金support from the National Natural Science Foundation of China(nos.81891010,81891013)the Key Project at central government level:The ability establishment of sustainable use for valuable Chinese medicine resources(no.2060302-1806-03)+1 种基金the High-level Teachers in Beijing Municipal Universities in the Period of 13th Five-year Plan(no.CIT&TCD20170324)the National Program for Special Support of Eminent Professionals.
文摘Panax notoginseng,a perennial herb of the genus Panax in the family Araliaceae,has played an important role in clinical treatment in China for thousands of years because of its extensive pharmacological effects.Here,we report a high-quality reference genome of P.notoginseng,with a genome size up to 2.66 Gb and a contig N50 of 1.12 Mb,produced with third-generation PacBio sequencing technology.This is the first chromosome-level genome assembly for the genus Panax.Through genome evolution analysis,we explored phylogenetic and whole-genome duplication events and examined their impact on saponin biosynthesis.We performed a detailed transcriptional analysis of P.notoginseng and explored genelevel mechanisms that regulate the formation of characteristic tubercles.Next,we studied the biosynthesis and regulation of saponins at temporal and spatial levels.We combined multi-omics data to identify genes that encode key enzymes in the P.notoginseng terpenoid biosynthetic pathway.Finally,we identified five glycosyltransferase genes whose products catalyzed the formation of different ginsenosides in P.notoginseng.The genetic information obtained in this study provides a resource for further exploration of the growth characteristics,cultivation,breeding,and saponin biosynthesis of P.notoginseng.
