Tissue engineering is an interdisciplinary field that integrates medical,biological,and engineering expertise to restore or regenerate the functionality of healthy tissues and organs.The three fundamental pillars of t...Tissue engineering is an interdisciplinary field that integrates medical,biological,and engineering expertise to restore or regenerate the functionality of healthy tissues and organs.The three fundamental pillars of tissue engineering are scaffolds,cells,and biomolecules.Electrospun nanofibers have been successfully used as scaffolds for a variety of tissue engineering applications because they are biomimetic of the natural,fibrous extracellular matrix(ECM)and contain a three-dimensional(3D)network of interconnected pores.In this review,we provide an overview of the electrospinning process,its principles,and the application of the resultant electrospun nanofibers for tissue engineering.We first briefly introduce the electrospinning process and then cover its principles and standard equipment for biomaterial fabrication.Next,we highlight the most important and recent advances related to the applications of electrospun nanofibers in tissue engineering,including skin,blood vessels,nerves,bone,cartilage,and tendon/ligament applications.Finally,we conclude with current advancements in the fabrication of electrospun nanofiber scaffolds and their biomedical applications in emerging areas.展开更多
Diabetic wound(DW)healing is a major clinical challenge due to multifactorial complications leading to prolonged inflammation.Electrospun nanofibrous(NF)membranes,due to special structural features,are promising bioma...Diabetic wound(DW)healing is a major clinical challenge due to multifactorial complications leading to prolonged inflammation.Electrospun nanofibrous(NF)membranes,due to special structural features,are promising biomaterials capable to promote DW healing through the delivery of active agents in a controlled manner.Herein,we report a multifunctional composite NF membrane loaded with ZnO nanoparticles(NP)and oregano essential oil(OEO),employing a new loading strategy,capable to sustainedly co-deliver bioactive agents.Physicochemical characterization revealed the successful fabrication of loaded nanofibers with strong in vitro anti-bacterial and anti-oxidant activities.Furthermore,in vivo wound healing confirmed the potential of bioactive NF membranes in epithelialization and granulation tissue formation.The angiogenesis was greatly prompted by the bioactive NF membranes through expression of vascular endothelial growth factor(VEGF).Moreover,the proposed NF membrane successfully terminated the inflammatory cycle by downregulating the pro-inflammatory cytokines interleukin6(IL-6)and matrix metalloproteinases-9(MMP-9).In vitro and in vivo studies revealed the proposed NF membrane is a promising dressing material for the healing of DW.展开更多
Scaffolds functionalized with graded changes in both fiber alignment and mineral content are more appealing for tendon-bone healing.This study reports the healing of rotator cuff injury using a heterogeneous nanofiber...Scaffolds functionalized with graded changes in both fiber alignment and mineral content are more appealing for tendon-bone healing.This study reports the healing of rotator cuff injury using a heterogeneous nanofiber scaffold,which is associated with a structural gradating from aligned to random and an increasing gradient of mineral content in the same orientation.The photothermal-triggered structural change of a nanofiber scaffold followed by graded mineralization is key to constructing such scaffolds.This type of scaffold was found to be biocompatible and provide beneficial contact guidance in the manipulation of tendon-derived stem cell morphologies in vitro.Specifically,tenogenic and osteogenic differentiation of tendon-derived stem cells were simultaneously achieved using the fabricated scaffold.In vivo investigation also showed the improved healing of rabbit rotator cuff injuries based on immunohistochemical analysis and biomechanical investigation that indicates the promising potential of a dual-gradient nanofiber scaffold in clinical tendon-bone healing.展开更多
Tissue injury leads to gradients of chemoattractants,which drive multiple processes for tissue repair,including the inflam-matory response as well as endogenous cell recruitment.However,a limited time window for the g...Tissue injury leads to gradients of chemoattractants,which drive multiple processes for tissue repair,including the inflam-matory response as well as endogenous cell recruitment.However,a limited time window for the gradients of chemoattract-ants as well as their poor stability at the injury site may not translate into healthy tissue repair.Consequently,intelligent multifunctional scaffolds with the capability to stabilize injury-induced cytokines and chemokines hold great promise for tissue repair.Vascular endothelial growth factor(VEGF)plays a significant role in wound healing by promoting angiogen-esis.The overarching objective of this research was to develop intelligent multifunctional scaffolds with the capability to endogenously recruit VEGF and promote wound healing via angiogenic and immunomodulatory dual functions.Prominin-1-derived peptide(PR1P)was encapsulated into electrospun poly(L-lactide-coglycolide)/gelatin(P/G)-based bandages.The sustained release of PR1P recruited VEGF in situ,thereby stabilizing the protein concentration peak in vivo and affording a reparative microenvironment with an adequate angiogenic ability at the wound site.Meanwhile,PR1P-recruited VEGF-induced macrophage reprogramming towards M2-like phenotypes further conferred immunomodulatory functions to the bandages.These dual functions of proangiogenesis and immunomodulation formed a cascade amplification,which regulated matrix metalloproteinases(MMP-9)as well as inflammatory factors(nuclear factor(NF)-κb,tumor necrosis factor(TNF)-α)in the wound microenvironment via the VEGF/macrophages/microenvironment axis.Consequently,the bandages realized multifunctional regeneration in splinted excisional wounds in rats,with or without diabetes,affording a higher skin append-age neogenesis,sensory function,and collagen remodeling.Conclusively,our approach encompassing in situ recruitment of VEGF at the injury site with the capability to promote immunomodulation-mediated tissue repair affords a promising avenue for scarless wound regeneration,which may also have implications for other tissue engineering disciplines.展开更多
Physiological repair of large-sized bone defects requires instructive scaffolds with appropriate mechanical properties,biocompatibility,biodegradability,vasculogenic ability and osteo-inductivity.The objective of this...Physiological repair of large-sized bone defects requires instructive scaffolds with appropriate mechanical properties,biocompatibility,biodegradability,vasculogenic ability and osteo-inductivity.The objective of this study was to fabricate in situ injectable hydrogels using platelet-rich plasma(PRP)-loaded gelatin methacrylate(GM)and employ them for the regeneration of large-sized bone defects.We performed various biological assays as well as assessed the mechanical properties of GM@PRP hydrogels alongside evaluating the release kinetics of growth factors(GFs)from hydrogels.The GM@PRP hydrogels manifested sufficient mechanical properties to support the filling of the tissue defects.For biofunction assay,the GM@PRP hydrogels significantly improved cell migration and angiogenesis.Especially,transcriptome RNA sequencing of human umbilical vein endothelial cells and bone marrow-derived stem cells were performed to delineate vascularization and biomineralization abilities of GM@PRP hydrogels.The GM@PRP hydrogels were subcutaneously implanted in rats for up to 4 weeks for preliminary biocompatibility followed by their transplantation into a tibial defect model for up to 8 weeks in rats.Tibial defects treated with GM@PRP hydrogels manifested significant bone regeneration as well as angiogenesis,biomineralization,and collagen deposition.Based on the biocompatibility and biological function of GM@PRP hydrogels,a new strategy is provided for the regenerative repair of large-size bone defects.展开更多
The construction of a smooth muscle layer for blood vessel through electrospinning method plays a key role in vascular tissue engineering.However,smooth muscle cells(SMCs)penetration into the electrospun graft to form...The construction of a smooth muscle layer for blood vessel through electrospinning method plays a key role in vascular tissue engineering.However,smooth muscle cells(SMCs)penetration into the electrospun graft to form a smooth muscle layer is limited due to the dense packing of fibers and lack of inducing factors.In this paper,silk fibroin/poly(L-lactide-e-caplacton)(SF/PLLA-CL)vascular graft loaded with platelet-rich growth factor(PRGF)was fabricated by electrospinning.The in vitro results showed that SMCs cultured in the graft grew fast,and the incorporation of PRGF could induce deeper SMCs infiltrating compared to the SF/PLLA-CL graft alone.Mechanical properties measurement showed that PRGF-incorporated graft had proper tensile stress,suture retention strength,burst pressure and compliance which could match the demand of native blood vessel.The success in the fabrication of PRGF-incorporated SF/PLLA-CL graft to induce fast SMCs growth and their strong penetration into graft has important application for tissue-engineered blood vessels.展开更多
The conjunctiva is crucial in safeguarding the eye from harm or infection,thereby ensuring the preservation of the vision.The repair of infected conjunctival damage is necessary.The objective of this study is to devel...The conjunctiva is crucial in safeguarding the eye from harm or infection,thereby ensuring the preservation of the vision.The repair of infected conjunctival damage is necessary.The objective of this study is to develop copper-doped flexible silica nanofibers(SiO_(2)@Cu NFs)with multifunctional antibacterial and anti-inflammatory characteristics.The continuous release of copper ions from electrospun membranes is shown to be effective to promote antibacterial and bioactive functions.Nanofiber membranes also exhibit biocompatibility and promote cell growth,angiogenesis,and inflammation modulation.In vivo evaluations further reveal the therapeutic efficacy of SiO_(2)@Cu NFs to promote the structural and the functional recoveries of the conjunctiva.Taken together,SiO_(2)@Cu NFs may hold significant promise for the fabrication of alterna-tive ocular bandage to suppress bacterial infection and promote repair of ocular tissues and may potential be also used for related disciplines.展开更多
Massive hemorrhage may be detrimental to the patients,which necessitates the advent of new materials with high hemostatic efficiency and good biocompatibility.The objective of this research was to screen for the effec...Massive hemorrhage may be detrimental to the patients,which necessitates the advent of new materials with high hemostatic efficiency and good biocompatibility.The objective of this research was to screen for the effect of the different types of bio-elastomers as hemostatic dressings.3D loose nanofiber sponges were prepared;PU-TA/Gel showed promising potential.Polyurethane(PU)was synthesized and electrospun to afford porous sponges,which were crosslinked with glutaraldehyde(GA).FTIR and 1H-NMR evidenced the successful synthesis of PU.The prepared PU-TA/Gel sponge had the highest porosity and water absorption ratio.Besides,PU-TA/Gel sponges exhibited cytocompatibility,negligible hemolysis and the shortest clotting time.PU-TA/Gel sponge rapidly induced stable blood clots with shorter hemostasis time and less bleeding volume in a liver injury model in rats.Intriguingly,PU-TA/Gel sponges also induced good skin regeneration in a full-thickness excisional defect model as revealed by the histological analysis.These results showed that the PU-TA/Gel-based sponges may offer an alternative platform for hemostasis and wound healing.展开更多
Correction to:Advanced Fiber Materials https://doi.org/10.1007/s42765-022-00226-8 In this article the author name Muhammad Shafiq was incorrectly written as Shafiq Muhammad.The original article has been corrected.
The rotator cuff is prone to tear under degenerative changes or mechanical injury,leading to excessive inflammation,extracellular matrix degradation,and unsatisfactory prognosis.Interleukin-4(IL-4)was used to induce m...The rotator cuff is prone to tear under degenerative changes or mechanical injury,leading to excessive inflammation,extracellular matrix degradation,and unsatisfactory prognosis.Interleukin-4(IL-4)was used to induce macrophages polarization toward M2 phenotype.By mapping IL 4-activated pathways and applying peptidome profiling,macrophage-derived peptide 1(MDP1)was identified and shown to promote the phos-phorylation of STAT3 and STAT6,thereby inducing the polarization of M0 macrophages toward the anti-inflammatory M2 phenotype.A functionally graded scaffold woven from electrospun nanofiber yarns was developed,with MDP1 and hydroxyapatite(HA)loaded onto its corresponding interfaces.During rotator cuff repair process,the scaffold functioned as an augmentation patch,with mechanical properties(Young’s modulus,ca.280 MPa)comparable to native tendons,prevented rotator cuff re-tearing in an early stage.MDP1 was incorporated into scaffolds to modulate an excessive inflammatory response,while HA was used to enhance bio-mineralization for enhanced osteointegration.Through a multidimensional collaborative repair strategy,this functionally graded scaffold not only mimicked the tendon-bone interface,but also significantly suppressed local inflammation at the interface,as evidenced by a 60.6%and 66.5%reduction in IL-6-positive areas at 2 and 4 months,respectively,compared with the control group.Furthermore,it promoted tissue regeneration in the damaged region,resulting in a 32.6%increase in Young’s modulus,thereby ultimately enhancing rotator cuff performance.The multifunctionally graded scaffold may offer an invaluable solution to promote rotator cuff tear healing and potentially other related disciplines.展开更多
Slugs could secrete mucus with multifunctional characteristics,such as reversible gelation,mucoadhesiveness,and viscoelasticity,which can be harnessed for multifaceted biotechnological and healthcare applications.The ...Slugs could secrete mucus with multifunctional characteristics,such as reversible gelation,mucoadhesiveness,and viscoelasticity,which can be harnessed for multifaceted biotechnological and healthcare applications.The dried mucus(DM)was prepared using slug,which can be adhered to the tissue surface through different types of interactions(lap-shear force,1.1 N for DM-3 group).The DM-3 further exhibited the highest hemostatic ability as discerned in a liver trauma injury model(hemostasis time,<15 s),biocompatibility and biodegradability(an insignificant residue at 4 weeks)in vivo,and considerably improved skin repair in full-thickness excisional wounds(wound closure,96.2%at day 14).Taken together,slug’s mucus can be easily prepared with an economic and an eco-friendly method,which may have broad biotechnological and healthcare implications and potential utility in other related disciplines.This transition from natural components to the biomaterial may provide an invaluable platform for different types of applications.展开更多
Electrospinning is widely accepted as a technique for the fabrication of nanofibrous three-dimensional(3D)scaffolds which mimic extracellular matrix(ECM)microenvironment for tissue engineering(TE).Unlike normal densel...Electrospinning is widely accepted as a technique for the fabrication of nanofibrous three-dimensional(3D)scaffolds which mimic extracellular matrix(ECM)microenvironment for tissue engineering(TE).Unlike normal densely-packed two-dimensional(2D)nanofibrous membranes,3D electrospun nanofiber scaffolds are dedicated to more precise spatial control,endowing the scaffolds with a sufficient porosity and 3D environment similar to the in vivo settings as well as optimizing the properties,including injectability,compressibility,and bioactivity.Moreover,the 3D morphology regulates cellular interaction and mediates growth,migration,and differentiation of cell for matrix remodeling.The variation among scaffold structures,functions and applications depends on the selection of electrospinning materials and methods as well as on the post-processing of electrospun scaffolds.This review summarizes the recent new forms for building electrospun 3D nanofiber scaffolds for TE applications.A variety of approaches aimed at the fabrication of 3D electrospun scaffolds,such as multilay-ering electrospinning,sacrificial agent electrospinning,wet electrospinning,ultrasound-enhanced electrospinning as well as post-processing techniques,including gas foaming,ultrasonication,short fiber assembly,3D printing,electrospraying,and so on are discussed,along with their advantages,limitations and applications.Meanwhile,the current challenges and prospects of 3D electrospun scaffolds are rationally discussed,providing an insight into developing the vibrant fields of biomedicine.展开更多
Electrospinning is a versatile strategy for creating nanofiber materials with various structures,which has broad application for a myriad of areas ranging from tissue engineering,energy harvesting,filtration and has b...Electrospinning is a versatile strategy for creating nanofiber materials with various structures,which has broad application for a myriad of areas ranging from tissue engineering,energy harvesting,filtration and has become one of the most important academic and technical activities in the field of material science in recent years.In addition to playing a significant role in the construction of two-dimensional(2D)nanomaterials,electrospinning holds great promise as a robust method for producing three-dimensional(3D)aerogels and scaffolds.This article reviews and summarizes the recent advanced methods for fabricating electrospun three-dimensional nanofiber aerogels and scaffolds,including gas foaming,direct electrospinning of 3D nanofibrous scaffold,short nanofibers assembling into 3D aerogels/scaffolds,3D printing,electrospray,origami and cell sheet engineering,centrifugal electrospinning,and other methods.Besides,intriguing formation process,crosslinking pathway,properties,and applications of 3D aerogels and scaffolds are also introduced.Taken together,these aerogels and scaffolds with various excellent features present tremendous potential in various fields.展开更多
Regeneration of Intervertebral disc(IVD)is a scientific challenge because of the complex structure and composition of tissue,as well as the difficulty in achieving bionic function.Here,an anatomically correct IVD scaf...Regeneration of Intervertebral disc(IVD)is a scientific challenge because of the complex structure and composition of tissue,as well as the difficulty in achieving bionic function.Here,an anatomically correct IVD scaffold composed of biomaterials,cells,and growth factors were fabricated via three-dimensional(3D)bioprinting technology.Connective tissue growth factor(CTGF)and transforming growth factor-β3(TGF-β3)were loaded onto polydopamine nanoparticles,which were mixed with bone marrow mesenchymal stem cells(BMSCs)for regenerating and simulating the structure and function of the nucleus pulposus and annular fibrosus.In vitro experiments confirmed that CTGF and TGF-β3 could be released from the IVD scaffold in a spatially controlled manner,and induced the corresponding BMSCs to differentiate into nucleus pulposus like cells and annulus fibrosus like cells.Next,the fabricated IVD scaffold was implanted into the dorsum subcutaneous of nude mice.The reconstructed IVD exhibited a zone-specific matrix that displayed the corresponding histological and immunological phenotypes:primarily type II collagen and glycosaminoglycan in the core zone,and type I collagen in the surrounding zone.The testing results demonstrated that it exhibited good biomechanical function of the reconstructed IVD.The results presented herein reveal the clinical application potential of the dual growth factors-releasing IVD scaffold fabricated via 3D bioprinting.However,the evaluation in large mammal animal models needs to be further studied.展开更多
It is a big challenge to develop a polyethylene terephthalate(PET)artificial ligament with excellent osteogenetic activity to enhance graft-bone integration for ligament reconstruction.Herein,we evaluated the effect o...It is a big challenge to develop a polyethylene terephthalate(PET)artificial ligament with excellent osteogenetic activity to enhance graft-bone integration for ligament reconstruction.Herein,we evaluated the effect of biomineralization(BM)and electrodeposition(ED)method for depositing calcium-phosphate(CaP)on the PET artificial ligament in vitro and in vivo.Scanning electron microscopy and energy-dispersive X-Ray spectrometer mapping analysis revealed that the ED-CaP had more uniform particles and element distribution(Ca,P and O),and thermogravimetric analysis showed there were more CaP on the PET/ED-CaP than the PET/BM-CaP scaffold.Moreover,the hydrophilicity of PET scaffolds was significantly improved after CaP deposition.In vitro study showed that CaP coating via BM or ED method could improve the attachment and proliferation of MC3T3-E1 cells,and ED-CaP coating significantly increased osteogenic differentiation of the cells,in which the Wnt/β-catenin signaling pathway might be involved.In addition,radiological,histological and immunohistochemical results of in vivo study in a rabbit anterior cruciate ligament(ACL)reconstruction model demonstrated that the PET/BM-CaP and PET/ED-CaP scaffolds significantly improved graft-bone integration process compared to the PET scaffold.More importantly,larger areas of new bone ingrowth and the formation of fibrocartilage tissue were observed at 12 weeks in the PET/ED-CaP group,and the biomechanical tests showed increased ultimate failure load and stiffness in PET/ED-CaP group compared to PET/BM-CaP and PET group.Therefore,ED of CaP is an effective strategy for the modification of PET artificial ligament and can enhance graft-bone integration both in vitro and in vivo.展开更多
The escalating prevalence of anterior cruciate ligament(ACL)injuries in sports necessitates innovative strategies for ACL reconstruction.In this study,we propose a multiphasic bone-ligament-bone(BLB)integrated scaffol...The escalating prevalence of anterior cruciate ligament(ACL)injuries in sports necessitates innovative strategies for ACL reconstruction.In this study,we propose a multiphasic bone-ligament-bone(BLB)integrated scaffold as a potential solution.The BLB scaffold comprised two polylactic acid(PLA)/deferoxamine(DFO)@mesoporous hydroxyapatite(MHA)thermally induced phase separation(TIPS)scaffolds bridged by silk fibroin(SF)/connective tissue growth factor(CTGF)@Poly(L-lactide-co-ε-caprolactone)(PLCL)nanofiber yarn braided scaffold.This combination mimics the native architecture of the ACL tissue.The mechanical properties of the BLB scaffolds were determined to be compatible with the human ACL.In vitro experiments demonstrated that CTGF induced the expression of ligament-related genes,while TIPS scaffolds loaded with MHA and DFO enhanced the osteogenic-related gene expression of bone marrow stem cells(BMSCs)and promoted the migration and tubular formation of human umbilical vein endothelial cells(HUVECs).In rabbit models,the BLB scaffold efficiently facilitated ligamentization and graft-bone integration processes by providing bioactive substances.The double delivery of DFO and calcium ions by the BLB scaffold synergistically promoted bone regeneration,while CTGF improved collagen formation and ligament healing.Collectively,the findings indicate that the BLB scaffold exhibits substantial promise for ACL reconstruction.Additional investigation and advancement of this scaffold may yield enhanced results in the management of ACL injuries.展开更多
The gelatin-glutaraldehyde （gelatin-GA） nanofibers were electrospun in order to overcome the defects of ex-situ crosslinking process such as complex process, destruction of fiber morphology and decrease of porosity....The gelatin-glutaraldehyde （gelatin-GA） nanofibers were electrospun in order to overcome the defects of ex-situ crosslinking process such as complex process, destruction of fiber morphology and decrease of porosity. The morphological structure, porosity, thermal property, moisture absorption and moisture retention performance, hydrolytic resistance, mechanical property and biocompatibiUty of nanofiber scaffolds were tested and characterized. The gelatin-GA nanofiber has nice uniform diameter and more than 80% porosity. The hydrolytic resistance and mechanical property of the gelatin-GA nanofiber scaffolds are greatly improved compared with that of gelatin nanofibers. The contact angle, moisture absorption, hydrolysis resistance, thermal resistance and mechanical property of gelatin-GA nanofiber scaffolds could be adjustable by varying the gelatin solution concentration and GA content. The gelatin- GA nanofibers had excellent properties, which are expected to be an ideal scaffold for biomedical and tissue engineering applications.展开更多
As one of the most common forms of skin injuries,skin burns are often accompanied by edema pain,suppuration of infection,slow tissue regeneration,and severe scar formation,which significantly delay wound healing as we...As one of the most common forms of skin injuries,skin burns are often accompanied by edema pain,suppuration of infection,slow tissue regeneration,and severe scar formation,which significantly delay wound healing as well as affect the quality of life.We prepared multifunctional electrospun poly(L-lactide-co-glycolide)/gelatin(P/G)-based dressings to synergistically harness the therapeutic benefits of peppermint essential oil(T),burn ointment(B),and Oregano essential oil(O)(P/G@TBO)for skin regeneration in punch and burn injury models.The P/G@TBO can afford the sustained release of bioactive cues for up to 72 h as well as remarkably promote cell migration(ca.P/G@TBO,89%vs.control group,51%)at 24 h.The P/G@TBO membranes also showed significant angiogenic effect as well as antibacterial and anti-inflammatory properties than that of the control group in vitro.Moreover,P/G@TBO dressings enabled fast wound healing(ca.P/G@TBO,100%wound closure vs.control group,95%)in a full-thickness excisional defect model up to 14 days in rats.Further evaluation of membranes in different animal models,including tail wagging model,facial itch model,and hot burn injury model showed significant pain relieve effect as well as itching and swelling relief functions during earlier stages of wound healing.Membranes were next transplanted into a scalded wound model in rats and an ear punch wound model in rabbits,which manifested antibacterial and anti-inflammatory properties and promoted re-epithelialization to achieve scarless wound healing percentage wound closure at day 28:P/G@TBO,96%vs.control group 66%.Taken together our approach of simultaneously harnessing T,B,and O to enable multifunctionality to fibrous dressings may hold great promise for burn wound healing applications and other related disciplines.展开更多
Tissue engineering focuses on repairing tissue and restoring tissue functions by employing three elements： scaffolds, cells and biochemical signals. In tissue engineering, bioactive material scaffolds have been used ...Tissue engineering focuses on repairing tissue and restoring tissue functions by employing three elements： scaffolds, cells and biochemical signals. In tissue engineering, bioactive material scaffolds have been used to cure tissue and organ defects with stem cell-based therapies being one of the best documented approaches. In the review, different biomaterials which are used in several methods to fabricate tissue engineering scaffolds were explained and show good properties （biocompatibility, biodegradability, and mechanical properties etc.） for cell migration and infiltration. Stem cell homing is a recruitment process for inducing the migration of the systemically transplanted cells, or host cells, to defect sites. The mechanisms and modes of stem cell homing-based tissue engineering can be divided into two types depending on the source of the stem cells： endogenous and exogenous. Exogenous stem cell-based bioactive scaffolds have the challenge of long-term culturing in vitro and for endogenous stem cells the biochemical signal homing recruitment mechanism is not clear yet. Although the stem cell homing-based bioactive scaffolds are attractive candidates for tissue defect therapies, based on in vitro studies and animal tests, there is still a long way before clinical application.展开更多
基金financially surpported by the Fundamental Research Funds for the Central Universities(No.2232019A3-07)the National Key Research Program of China(Nos.2016YFA0201702 of 2016YFA0201700)+2 种基金the National Nature Science Foundation of China(No.31771023)the Science and Technology Commission of Shanghai Municipality(No.19441902600)the Fundamental Research Funds for the Central Universities and Graduate Student Innovation Fund of Donghua University(No.CUSF-DH-D-2020061)。
文摘Tissue engineering is an interdisciplinary field that integrates medical,biological,and engineering expertise to restore or regenerate the functionality of healthy tissues and organs.The three fundamental pillars of tissue engineering are scaffolds,cells,and biomolecules.Electrospun nanofibers have been successfully used as scaffolds for a variety of tissue engineering applications because they are biomimetic of the natural,fibrous extracellular matrix(ECM)and contain a three-dimensional(3D)network of interconnected pores.In this review,we provide an overview of the electrospinning process,its principles,and the application of the resultant electrospun nanofibers for tissue engineering.We first briefly introduce the electrospinning process and then cover its principles and standard equipment for biomaterial fabrication.Next,we highlight the most important and recent advances related to the applications of electrospun nanofibers in tissue engineering,including skin,blood vessels,nerves,bone,cartilage,and tendon/ligament applications.Finally,we conclude with current advancements in the fabrication of electrospun nanofiber scaffolds and their biomedical applications in emerging areas.
基金This research was supported by the Fundamental Research Funds for the Central Universities(2232019A3-07)National Key Research Program of China(2016YFC1100202)+1 种基金National Natural Science Foundation of China(No.31771023)Science and Technology Commission of Shanghai Municipality(No.19441902600,20S31900900).
文摘Diabetic wound(DW)healing is a major clinical challenge due to multifactorial complications leading to prolonged inflammation.Electrospun nanofibrous(NF)membranes,due to special structural features,are promising biomaterials capable to promote DW healing through the delivery of active agents in a controlled manner.Herein,we report a multifunctional composite NF membrane loaded with ZnO nanoparticles(NP)and oregano essential oil(OEO),employing a new loading strategy,capable to sustainedly co-deliver bioactive agents.Physicochemical characterization revealed the successful fabrication of loaded nanofibers with strong in vitro anti-bacterial and anti-oxidant activities.Furthermore,in vivo wound healing confirmed the potential of bioactive NF membranes in epithelialization and granulation tissue formation.The angiogenesis was greatly prompted by the bioactive NF membranes through expression of vascular endothelial growth factor(VEGF).Moreover,the proposed NF membrane successfully terminated the inflammatory cycle by downregulating the pro-inflammatory cytokines interleukin6(IL-6)and matrix metalloproteinases-9(MMP-9).In vitro and in vivo studies revealed the proposed NF membrane is a promising dressing material for the healing of DW.
基金This research was supported by National Natural Science Foundation of China(31872310,82001970)Natural Science Foundation of Shandong Province(ZR2019MH097,ZR2021YQ17)+1 种基金Young Elite Scientists Sponsorship Program by CAST(No.YESS20200097)startup funds from Qingdao University(T.W.).
文摘Scaffolds functionalized with graded changes in both fiber alignment and mineral content are more appealing for tendon-bone healing.This study reports the healing of rotator cuff injury using a heterogeneous nanofiber scaffold,which is associated with a structural gradating from aligned to random and an increasing gradient of mineral content in the same orientation.The photothermal-triggered structural change of a nanofiber scaffold followed by graded mineralization is key to constructing such scaffolds.This type of scaffold was found to be biocompatible and provide beneficial contact guidance in the manipulation of tendon-derived stem cell morphologies in vitro.Specifically,tenogenic and osteogenic differentiation of tendon-derived stem cells were simultaneously achieved using the fabricated scaffold.In vivo investigation also showed the improved healing of rabbit rotator cuff injuries based on immunohistochemical analysis and biomechanical investigation that indicates the promising potential of a dual-gradient nanofiber scaffold in clinical tendon-bone healing.
基金Funding National Natural Science Foundation of China,81770091,Chang Chen,NSFC32050410286Shafiq Muhammad,Science and Technology Innovation Plan Of Shanghai Science and Technology Commission,No.20DZ2253700+2 种基金Chang Chen,Japan Society for the Promotion of Science,JP21F21353Shafiq Muhammad,Sino German Science Foundation Research Exchange Center,M-0263Xiumei Mo,Science and Technology Commission of Shanghai Municipality,20S31900900,Xiumei Mo,20DZ2254900,Xiumei Mo.
文摘Tissue injury leads to gradients of chemoattractants,which drive multiple processes for tissue repair,including the inflam-matory response as well as endogenous cell recruitment.However,a limited time window for the gradients of chemoattract-ants as well as their poor stability at the injury site may not translate into healthy tissue repair.Consequently,intelligent multifunctional scaffolds with the capability to stabilize injury-induced cytokines and chemokines hold great promise for tissue repair.Vascular endothelial growth factor(VEGF)plays a significant role in wound healing by promoting angiogen-esis.The overarching objective of this research was to develop intelligent multifunctional scaffolds with the capability to endogenously recruit VEGF and promote wound healing via angiogenic and immunomodulatory dual functions.Prominin-1-derived peptide(PR1P)was encapsulated into electrospun poly(L-lactide-coglycolide)/gelatin(P/G)-based bandages.The sustained release of PR1P recruited VEGF in situ,thereby stabilizing the protein concentration peak in vivo and affording a reparative microenvironment with an adequate angiogenic ability at the wound site.Meanwhile,PR1P-recruited VEGF-induced macrophage reprogramming towards M2-like phenotypes further conferred immunomodulatory functions to the bandages.These dual functions of proangiogenesis and immunomodulation formed a cascade amplification,which regulated matrix metalloproteinases(MMP-9)as well as inflammatory factors(nuclear factor(NF)-κb,tumor necrosis factor(TNF)-α)in the wound microenvironment via the VEGF/macrophages/microenvironment axis.Consequently,the bandages realized multifunctional regeneration in splinted excisional wounds in rats,with or without diabetes,affording a higher skin append-age neogenesis,sensory function,and collagen remodeling.Conclusively,our approach encompassing in situ recruitment of VEGF at the injury site with the capability to promote immunomodulation-mediated tissue repair affords a promising avenue for scarless wound regeneration,which may also have implications for other tissue engineering disciplines.
基金funded by Donghua University Postgraduate Innovation and Entrepreneurship Ability Training Program(yjssc2023002)supported by Science and Technology Commission of Shanghai Municipality,China(grant numbers 20S31900900 and 20DZ2254900)+1 种基金Sino German Science Foundation Research Exchange Center,China(M-0263)China Education Association for International Exchange(2022181).
文摘Physiological repair of large-sized bone defects requires instructive scaffolds with appropriate mechanical properties,biocompatibility,biodegradability,vasculogenic ability and osteo-inductivity.The objective of this study was to fabricate in situ injectable hydrogels using platelet-rich plasma(PRP)-loaded gelatin methacrylate(GM)and employ them for the regeneration of large-sized bone defects.We performed various biological assays as well as assessed the mechanical properties of GM@PRP hydrogels alongside evaluating the release kinetics of growth factors(GFs)from hydrogels.The GM@PRP hydrogels manifested sufficient mechanical properties to support the filling of the tissue defects.For biofunction assay,the GM@PRP hydrogels significantly improved cell migration and angiogenesis.Especially,transcriptome RNA sequencing of human umbilical vein endothelial cells and bone marrow-derived stem cells were performed to delineate vascularization and biomineralization abilities of GM@PRP hydrogels.The GM@PRP hydrogels were subcutaneously implanted in rats for up to 4 weeks for preliminary biocompatibility followed by their transplantation into a tibial defect model for up to 8 weeks in rats.Tibial defects treated with GM@PRP hydrogels manifested significant bone regeneration as well as angiogenesis,biomineralization,and collagen deposition.Based on the biocompatibility and biological function of GM@PRP hydrogels,a new strategy is provided for the regenerative repair of large-size bone defects.
基金This work was supported by National Natural Science Foundation of China(31500784)China Postdoctoral Science Foundation Funded Project(2015M580790)Program of Introducing Talents of Discipline to Universities(B16033).
文摘The construction of a smooth muscle layer for blood vessel through electrospinning method plays a key role in vascular tissue engineering.However,smooth muscle cells(SMCs)penetration into the electrospun graft to form a smooth muscle layer is limited due to the dense packing of fibers and lack of inducing factors.In this paper,silk fibroin/poly(L-lactide-e-caplacton)(SF/PLLA-CL)vascular graft loaded with platelet-rich growth factor(PRGF)was fabricated by electrospinning.The in vitro results showed that SMCs cultured in the graft grew fast,and the incorporation of PRGF could induce deeper SMCs infiltrating compared to the SF/PLLA-CL graft alone.Mechanical properties measurement showed that PRGF-incorporated graft had proper tensile stress,suture retention strength,burst pressure and compliance which could match the demand of native blood vessel.The success in the fabrication of PRGF-incorporated SF/PLLA-CL graft to induce fast SMCs growth and their strong penetration into graft has important application for tissue-engineered blood vessels.
基金supported by Science and Technology Commission of Shanghai Municipality,China(Nos.20S31900900,20DZ2254900)Sino German Science Foundation Research Exchange Center,China(M-0263)+7 种基金China Education Association for International Exchange(2022181)supported by Researchers Supporting Project Number(RSP2024R65)King Saud University,Riyadh,Saudi Arabia.Moreover,this project was supported by the State Key Laboratory for Modification of Chemical Fibers and Polymer Materials,Donghua University(KF2109)National Natural Science Foundation of China(No.82070919 and 82271041)the Program of Shanghai Academic/Technology Research Leader(22XD1401800)the Biomaterials and Regenerative Medicine Institute Cooperative Research Project,Shanghai Jiao Tong University School of Medicine(2022LHA06)Shanghai Key Clinical Specialty,and Shanghai Eye Disease Research Center(2022ZZ01003)supported by the Fundamental Research Funds for the Central Universities(CUSF-DH-T-2023064).
文摘The conjunctiva is crucial in safeguarding the eye from harm or infection,thereby ensuring the preservation of the vision.The repair of infected conjunctival damage is necessary.The objective of this study is to develop copper-doped flexible silica nanofibers(SiO_(2)@Cu NFs)with multifunctional antibacterial and anti-inflammatory characteristics.The continuous release of copper ions from electrospun membranes is shown to be effective to promote antibacterial and bioactive functions.Nanofiber membranes also exhibit biocompatibility and promote cell growth,angiogenesis,and inflammation modulation.In vivo evaluations further reveal the therapeutic efficacy of SiO_(2)@Cu NFs to promote the structural and the functional recoveries of the conjunctiva.Taken together,SiO_(2)@Cu NFs may hold significant promise for the fabrication of alterna-tive ocular bandage to suppress bacterial infection and promote repair of ocular tissues and may potential be also used for related disciplines.
基金supported by the Science and Technology Commission of Shanghai Municipality(19441902600,20S31900900,20DZ2254900)Sino German Science Foundation Research Exchange Center(M-0263)+3 种基金the crossdisciplinary project of Donghua University(101080241022)This project was also supported by Researchers Supporting Project Number(RSP2023R65)King Saud University,Riyadh,Saudi Arabia.M.S.is an International Research Fellow of the Japan Society for the Promotion of Science(Postdoctoral Fellowships for Research in Japan(Standard))The part of this research was also funded by Grant-in-Aid for JSPS Fellows(JP21F21353).
文摘Massive hemorrhage may be detrimental to the patients,which necessitates the advent of new materials with high hemostatic efficiency and good biocompatibility.The objective of this research was to screen for the effect of the different types of bio-elastomers as hemostatic dressings.3D loose nanofiber sponges were prepared;PU-TA/Gel showed promising potential.Polyurethane(PU)was synthesized and electrospun to afford porous sponges,which were crosslinked with glutaraldehyde(GA).FTIR and 1H-NMR evidenced the successful synthesis of PU.The prepared PU-TA/Gel sponge had the highest porosity and water absorption ratio.Besides,PU-TA/Gel sponges exhibited cytocompatibility,negligible hemolysis and the shortest clotting time.PU-TA/Gel sponge rapidly induced stable blood clots with shorter hemostasis time and less bleeding volume in a liver injury model in rats.Intriguingly,PU-TA/Gel sponges also induced good skin regeneration in a full-thickness excisional defect model as revealed by the histological analysis.These results showed that the PU-TA/Gel-based sponges may offer an alternative platform for hemostasis and wound healing.
文摘Correction to:Advanced Fiber Materials https://doi.org/10.1007/s42765-022-00226-8 In this article the author name Muhammad Shafiq was incorrectly written as Shafiq Muhammad.The original article has been corrected.
基金supported by the Medical-Engineering Interdisci-plinary Collaborative Project(Grants No.2023DHYGJC-YBB04)be-tween Shanghai Tongren Hospital and Donghua UniversityFundamental Research Funds for the Central Universities(project number YG2024QNA62)+6 种基金the Program of Shanghai Municipal Commis-sion of Health(Grants No.20224Y0092)the Laboratory Open Fund of Key Technology and Materials in Minimally Invasive Spine Surgery(Grants No.2024JZWC-YBB06)supported by Sci-ence and Technology Commission of Shanghai Municipality,China(20DZ2254900)Sino German Science Foundation Research Exchange Center,China(M-0263)China Education Association for Interna-tional Exchange(2022181)supported by Ongoing Research Funding program,(ORF-2025-65)King Saud Uni-versity,Riyadh,Saudi Arabia.
文摘The rotator cuff is prone to tear under degenerative changes or mechanical injury,leading to excessive inflammation,extracellular matrix degradation,and unsatisfactory prognosis.Interleukin-4(IL-4)was used to induce macrophages polarization toward M2 phenotype.By mapping IL 4-activated pathways and applying peptidome profiling,macrophage-derived peptide 1(MDP1)was identified and shown to promote the phos-phorylation of STAT3 and STAT6,thereby inducing the polarization of M0 macrophages toward the anti-inflammatory M2 phenotype.A functionally graded scaffold woven from electrospun nanofiber yarns was developed,with MDP1 and hydroxyapatite(HA)loaded onto its corresponding interfaces.During rotator cuff repair process,the scaffold functioned as an augmentation patch,with mechanical properties(Young’s modulus,ca.280 MPa)comparable to native tendons,prevented rotator cuff re-tearing in an early stage.MDP1 was incorporated into scaffolds to modulate an excessive inflammatory response,while HA was used to enhance bio-mineralization for enhanced osteointegration.Through a multidimensional collaborative repair strategy,this functionally graded scaffold not only mimicked the tendon-bone interface,but also significantly suppressed local inflammation at the interface,as evidenced by a 60.6%and 66.5%reduction in IL-6-positive areas at 2 and 4 months,respectively,compared with the control group.Furthermore,it promoted tissue regeneration in the damaged region,resulting in a 32.6%increase in Young’s modulus,thereby ultimately enhancing rotator cuff performance.The multifunctionally graded scaffold may offer an invaluable solution to promote rotator cuff tear healing and potentially other related disciplines.
基金supported by Taishan Scholars Program of Shandong Province(tsqn201812141)Shandong Provincial Natural Science Foundation(ZR2021MH004)+7 种基金Science and Technology Commission of Shanghai Municipality,China(No.20DZ2254900)Sino German Science Foundation Research Exchange Center,China(M-0263)China Education Association for International Exchange(2022181)supported by Researchers Supporting Project Number(RSP2025R65)King Saud University,Riyadh,Saudi Arabia,the Science and Technology Research Program of Chongqing Municipal Education Commission(Grant No.KJZD-K202412807)Youth Doctoral Talent Incubation Program of the Second Affiliated Hospital,Army Medical University(2023YQB002)supported by China Postdoctoral Science Foundation(2023M742325)the Fundamental Research Funds for the Central Universities and Graduate Student Innovation Fund of Donghua University(CUSF-DH-D-2024040)。
文摘Slugs could secrete mucus with multifunctional characteristics,such as reversible gelation,mucoadhesiveness,and viscoelasticity,which can be harnessed for multifaceted biotechnological and healthcare applications.The dried mucus(DM)was prepared using slug,which can be adhered to the tissue surface through different types of interactions(lap-shear force,1.1 N for DM-3 group).The DM-3 further exhibited the highest hemostatic ability as discerned in a liver trauma injury model(hemostasis time,<15 s),biocompatibility and biodegradability(an insignificant residue at 4 weeks)in vivo,and considerably improved skin repair in full-thickness excisional wounds(wound closure,96.2%at day 14).Taken together,slug’s mucus can be easily prepared with an economic and an eco-friendly method,which may have broad biotechnological and healthcare implications and potential utility in other related disciplines.This transition from natural components to the biomaterial may provide an invaluable platform for different types of applications.
基金The authors would like to thank the financial support from National Nature Science Foundation of China(No.32050410286)Science and Technology Commission of Shanghai Municipality(No.20S31900900,20DZ2254900)+2 种基金Sino German Science Foundation Research Exchange Center(M-0263)National Advanced Functional Fiber Innovation Center(2021-fx020301)International Cooperation of 2021-2022 China and Poland Science and Technology Personnel Exchange Program(No.17).
文摘Electrospinning is widely accepted as a technique for the fabrication of nanofibrous three-dimensional(3D)scaffolds which mimic extracellular matrix(ECM)microenvironment for tissue engineering(TE).Unlike normal densely-packed two-dimensional(2D)nanofibrous membranes,3D electrospun nanofiber scaffolds are dedicated to more precise spatial control,endowing the scaffolds with a sufficient porosity and 3D environment similar to the in vivo settings as well as optimizing the properties,including injectability,compressibility,and bioactivity.Moreover,the 3D morphology regulates cellular interaction and mediates growth,migration,and differentiation of cell for matrix remodeling.The variation among scaffold structures,functions and applications depends on the selection of electrospinning materials and methods as well as on the post-processing of electrospun scaffolds.This review summarizes the recent new forms for building electrospun 3D nanofiber scaffolds for TE applications.A variety of approaches aimed at the fabrication of 3D electrospun scaffolds,such as multilay-ering electrospinning,sacrificial agent electrospinning,wet electrospinning,ultrasound-enhanced electrospinning as well as post-processing techniques,including gas foaming,ultrasonication,short fiber assembly,3D printing,electrospraying,and so on are discussed,along with their advantages,limitations and applications.Meanwhile,the current challenges and prospects of 3D electrospun scaffolds are rationally discussed,providing an insight into developing the vibrant fields of biomedicine.
基金supported by the Fundamental Research Funds for the Central Universities(Grant No.2232019A3-07)National Key Research Program of China(2016YFC1100202)+2 种基金National Natural Science Foundation of China(No.31771023)Science and Technology Commission of Shanghai Municipality(No.19441902600)a startup research grant of Higher Education Commission(HEC),Pakistan(Project No.2089).
文摘Electrospinning is a versatile strategy for creating nanofiber materials with various structures,which has broad application for a myriad of areas ranging from tissue engineering,energy harvesting,filtration and has become one of the most important academic and technical activities in the field of material science in recent years.In addition to playing a significant role in the construction of two-dimensional(2D)nanomaterials,electrospinning holds great promise as a robust method for producing three-dimensional(3D)aerogels and scaffolds.This article reviews and summarizes the recent advanced methods for fabricating electrospun three-dimensional nanofiber aerogels and scaffolds,including gas foaming,direct electrospinning of 3D nanofibrous scaffold,short nanofibers assembling into 3D aerogels/scaffolds,3D printing,electrospray,origami and cell sheet engineering,centrifugal electrospinning,and other methods.Besides,intriguing formation process,crosslinking pathway,properties,and applications of 3D aerogels and scaffolds are also introduced.Taken together,these aerogels and scaffolds with various excellent features present tremendous potential in various fields.
基金supported by National Key R&D Program of China(No.2018YFB1105600,No.2018YFA0703000)National Natural Science Foundation of China(No.81802131)Project funded by China Postdoctoral Science Foundation(No.2019T120347)and the fund of No.XK2019013.
文摘Regeneration of Intervertebral disc(IVD)is a scientific challenge because of the complex structure and composition of tissue,as well as the difficulty in achieving bionic function.Here,an anatomically correct IVD scaffold composed of biomaterials,cells,and growth factors were fabricated via three-dimensional(3D)bioprinting technology.Connective tissue growth factor(CTGF)and transforming growth factor-β3(TGF-β3)were loaded onto polydopamine nanoparticles,which were mixed with bone marrow mesenchymal stem cells(BMSCs)for regenerating and simulating the structure and function of the nucleus pulposus and annular fibrosus.In vitro experiments confirmed that CTGF and TGF-β3 could be released from the IVD scaffold in a spatially controlled manner,and induced the corresponding BMSCs to differentiate into nucleus pulposus like cells and annulus fibrosus like cells.Next,the fabricated IVD scaffold was implanted into the dorsum subcutaneous of nude mice.The reconstructed IVD exhibited a zone-specific matrix that displayed the corresponding histological and immunological phenotypes:primarily type II collagen and glycosaminoglycan in the core zone,and type I collagen in the surrounding zone.The testing results demonstrated that it exhibited good biomechanical function of the reconstructed IVD.The results presented herein reveal the clinical application potential of the dual growth factors-releasing IVD scaffold fabricated via 3D bioprinting.However,the evaluation in large mammal animal models needs to be further studied.
基金supported by the National Key Research and Development Program of China(Grant No.2018YFC1106200 ,2018YFC1106202)the National Natural Science Foundation of China(Grant No.81871753 , 81772341)+1 种基金China Postdoctoral Science Foundation(Grant No.2020M671154)the Fundamental Research Funds for the Central Universities and Graduate Student Innovation Fund of Donghua University(Grant No.CUSF-DH-D-2019070).
文摘It is a big challenge to develop a polyethylene terephthalate(PET)artificial ligament with excellent osteogenetic activity to enhance graft-bone integration for ligament reconstruction.Herein,we evaluated the effect of biomineralization(BM)and electrodeposition(ED)method for depositing calcium-phosphate(CaP)on the PET artificial ligament in vitro and in vivo.Scanning electron microscopy and energy-dispersive X-Ray spectrometer mapping analysis revealed that the ED-CaP had more uniform particles and element distribution(Ca,P and O),and thermogravimetric analysis showed there were more CaP on the PET/ED-CaP than the PET/BM-CaP scaffold.Moreover,the hydrophilicity of PET scaffolds was significantly improved after CaP deposition.In vitro study showed that CaP coating via BM or ED method could improve the attachment and proliferation of MC3T3-E1 cells,and ED-CaP coating significantly increased osteogenic differentiation of the cells,in which the Wnt/β-catenin signaling pathway might be involved.In addition,radiological,histological and immunohistochemical results of in vivo study in a rabbit anterior cruciate ligament(ACL)reconstruction model demonstrated that the PET/BM-CaP and PET/ED-CaP scaffolds significantly improved graft-bone integration process compared to the PET scaffold.More importantly,larger areas of new bone ingrowth and the formation of fibrocartilage tissue were observed at 12 weeks in the PET/ED-CaP group,and the biomechanical tests showed increased ultimate failure load and stiffness in PET/ED-CaP group compared to PET/BM-CaP and PET group.Therefore,ED of CaP is an effective strategy for the modification of PET artificial ligament and can enhance graft-bone integration both in vitro and in vivo.
基金supported by the Fundamental Research Funds for the Central Universities(2232019A3-07,2232019D3-20)the Science and Technology Commission of Shanghai Municipality(20S31900900,20DZ2254900)+8 种基金the Sino German Science Foundation Research Exchange Center(M-0263)the National Natural Science Foundation of China(82102579,81871753)the Shanghai Rising-Star Program(22QC1401200)the Basic Scientific Research Project of Shanghai Sixth People’s Hospital(ynqn202101)the Opening Project of National Engineering Laboratory for Modern Silk,Soochow University(SDGC2149)the National Key Research and Development Program of China(2018YFC1106200,2018YFC1106202)the Key R&D Program of Shandong province(2019JZZY011104)the Deutsche Forschungsgemeinschaft(German Research Foundation(B.R.:RO 2511/11-1)),This project was also supported by Researchers Supporting Project Number(RSP2023R65)King Saud University,Riyadh,Saudi Arabia,and the combined Sino-German Mobility Programme of the National Natural Science Foundation of China(NSFC)/Deutsche Forschungsgemeinschaft(German Research Foundation(B.R.:M-0332).
文摘The escalating prevalence of anterior cruciate ligament(ACL)injuries in sports necessitates innovative strategies for ACL reconstruction.In this study,we propose a multiphasic bone-ligament-bone(BLB)integrated scaffold as a potential solution.The BLB scaffold comprised two polylactic acid(PLA)/deferoxamine(DFO)@mesoporous hydroxyapatite(MHA)thermally induced phase separation(TIPS)scaffolds bridged by silk fibroin(SF)/connective tissue growth factor(CTGF)@Poly(L-lactide-co-ε-caprolactone)(PLCL)nanofiber yarn braided scaffold.This combination mimics the native architecture of the ACL tissue.The mechanical properties of the BLB scaffolds were determined to be compatible with the human ACL.In vitro experiments demonstrated that CTGF induced the expression of ligament-related genes,while TIPS scaffolds loaded with MHA and DFO enhanced the osteogenic-related gene expression of bone marrow stem cells(BMSCs)and promoted the migration and tubular formation of human umbilical vein endothelial cells(HUVECs).In rabbit models,the BLB scaffold efficiently facilitated ligamentization and graft-bone integration processes by providing bioactive substances.The double delivery of DFO and calcium ions by the BLB scaffold synergistically promoted bone regeneration,while CTGF improved collagen formation and ligament healing.Collectively,the findings indicate that the BLB scaffold exhibits substantial promise for ACL reconstruction.Additional investigation and advancement of this scaffold may yield enhanced results in the management of ACL injuries.
基金Acknowledgements This research was supported by the National Natural Science Foundation of China （Grant Nos. 31470941 and 31271035）, the Innovation Fund Designated for Graduate Students of Donghua University （Item No. CUSF-DH-D-2015032）, Science and Technology Commission of Shanghai Municipality （15JC1490100, 15441905100）, Ph.D. Programs Foundation of Ministry of Education of China （20130075110005） and light of textile project 0201404）, Technology Bureau of Jiaxing City （MTC2012- 006, 2011A Y1026）, Science and Technology Agency of Zhejiang Province （2012R10012-09, 2010R50012-19）. The authors would like to extend their sincere appreciation to the Deanship of Scientific Research at King Saud University for its funding of this research through the research group project No. RGP-201.
文摘The gelatin-glutaraldehyde （gelatin-GA） nanofibers were electrospun in order to overcome the defects of ex-situ crosslinking process such as complex process, destruction of fiber morphology and decrease of porosity. The morphological structure, porosity, thermal property, moisture absorption and moisture retention performance, hydrolytic resistance, mechanical property and biocompatibiUty of nanofiber scaffolds were tested and characterized. The gelatin-GA nanofiber has nice uniform diameter and more than 80% porosity. The hydrolytic resistance and mechanical property of the gelatin-GA nanofiber scaffolds are greatly improved compared with that of gelatin nanofibers. The contact angle, moisture absorption, hydrolysis resistance, thermal resistance and mechanical property of gelatin-GA nanofiber scaffolds could be adjustable by varying the gelatin solution concentration and GA content. The gelatin- GA nanofibers had excellent properties, which are expected to be an ideal scaffold for biomedical and tissue engineering applications.
基金Science and Technology Commission of Shanghai Municipality(No.20S31900900,20DZ2254900)Sino German Science Foundation Research Exchange Center(M-0263)+8 种基金Taishan Scholars Program of Shandong Province(tsqn201812141)Shandong Provincial Natural Science Foundation(ZR2021MH004)Academic promotion program of Shandong First Medical University(2019RC016)Researchers Supporting Project Number(RSP2023R65)King Saud University,Riyadh,Saudi Arabia,China Education Association for International Exchange(2022181)Donghua University Postgraduate Innovation and Entrepreneurship Ability Training Program(yjssc2023002)Grant-in-Aid for JSPS Research Fellows(JP21F21353)M.S.is an International Research Fellow of the Japan Society for the Promotion of Science(Postdoctoral Fellowships for Research in Japan(Standard))M.S.is keenly grateful to the Gifu University,Japan for Special Cooperative Research Program and College of Biological Science and Medical Engineering,Donghua University,Shanghai,China for cooperative research.
文摘As one of the most common forms of skin injuries,skin burns are often accompanied by edema pain,suppuration of infection,slow tissue regeneration,and severe scar formation,which significantly delay wound healing as well as affect the quality of life.We prepared multifunctional electrospun poly(L-lactide-co-glycolide)/gelatin(P/G)-based dressings to synergistically harness the therapeutic benefits of peppermint essential oil(T),burn ointment(B),and Oregano essential oil(O)(P/G@TBO)for skin regeneration in punch and burn injury models.The P/G@TBO can afford the sustained release of bioactive cues for up to 72 h as well as remarkably promote cell migration(ca.P/G@TBO,89%vs.control group,51%)at 24 h.The P/G@TBO membranes also showed significant angiogenic effect as well as antibacterial and anti-inflammatory properties than that of the control group in vitro.Moreover,P/G@TBO dressings enabled fast wound healing(ca.P/G@TBO,100%wound closure vs.control group,95%)in a full-thickness excisional defect model up to 14 days in rats.Further evaluation of membranes in different animal models,including tail wagging model,facial itch model,and hot burn injury model showed significant pain relieve effect as well as itching and swelling relief functions during earlier stages of wound healing.Membranes were next transplanted into a scalded wound model in rats and an ear punch wound model in rabbits,which manifested antibacterial and anti-inflammatory properties and promoted re-epithelialization to achieve scarless wound healing percentage wound closure at day 28:P/G@TBO,96%vs.control group 66%.Taken together our approach of simultaneously harnessing T,B,and O to enable multifunctionality to fibrous dressings may hold great promise for burn wound healing applications and other related disciplines.
文摘Tissue engineering focuses on repairing tissue and restoring tissue functions by employing three elements： scaffolds, cells and biochemical signals. In tissue engineering, bioactive material scaffolds have been used to cure tissue and organ defects with stem cell-based therapies being one of the best documented approaches. In the review, different biomaterials which are used in several methods to fabricate tissue engineering scaffolds were explained and show good properties （biocompatibility, biodegradability, and mechanical properties etc.） for cell migration and infiltration. Stem cell homing is a recruitment process for inducing the migration of the systemically transplanted cells, or host cells, to defect sites. The mechanisms and modes of stem cell homing-based tissue engineering can be divided into two types depending on the source of the stem cells： endogenous and exogenous. Exogenous stem cell-based bioactive scaffolds have the challenge of long-term culturing in vitro and for endogenous stem cells the biochemical signal homing recruitment mechanism is not clear yet. Although the stem cell homing-based bioactive scaffolds are attractive candidates for tissue defect therapies, based on in vitro studies and animal tests, there is still a long way before clinical application.
