Purpose:To evaluate the feasibility and efficacy of a transmesenteric vein extrahepatic portosystemic shunt(TmEPS)for the treatment of cavernous transformation of the portal vein(CTPV).Materials and methods:The clinic...Purpose:To evaluate the feasibility and efficacy of a transmesenteric vein extrahepatic portosystemic shunt(TmEPS)for the treatment of cavernous transformation of the portal vein(CTPV).Materials and methods:The clinical data of 20 patients with CTPV who underwent TmEPS between December 2020and January 2022 at Henan Provincial People’s Hospital were retrospectively collected.The superior mesenteric vein(SMV)trunk was patent or partially occluded in these patients.An extrahepatic portosystemic shunt between the inferior vena cava and the SMV was established using a stent graft through an infraumbilical median longitudinal mini-laparotomy.The technical success,efficacy,and complication rates were evaluated,and the preand postoperative SMV pressures were compared.Patients’clinical outcomes and shunt patency were assessed.Results:TmEPS was successfully performed in 20 patients.The initial puncture success rate of the balloon-assisted puncture technique is 95%.The mean SMV pressure decreased from 29.1±2.9 mmHg to 15.6±3.3 mmHg(p<0.001).All symptoms of portal hypertension resolved.No fatal procedural complications occurred.During the follow-up period,hepatic encephalopathy occurred in two patients.The remaining patients remained asymptomatic.All shunts were patent.Conclusions:TmEPS is a feasible,safe,and effective treatment option for patients with CTPV.展开更多
Anatase TiO_(2) is a promising anode material for lithium-ion batteries(LIBs)owing to its low cost and stability.However,the intrinsically kinetic limits seriously hindered its lithium-ion storage capability.Here we p...Anatase TiO_(2) is a promising anode material for lithium-ion batteries(LIBs)owing to its low cost and stability.However,the intrinsically kinetic limits seriously hindered its lithium-ion storage capability.Here we present that anatase TiO_(2) with rich oxygen vacancies can enhance its lithium-ion storage performance.We synthesize anatase TiO_(2) with well-retained hierarchical structure by annealing the H_(2)Ti_(5)O_(11)·3H_(2)O yolk-shell spheres precursor in nitrogen atmosphere.EPR and XPS data evidence that the oxygen-deficient environment could generate abundant oxygen vacancies in the as-derived anatase TiO_(2),which leads to improved electron conductivity and reduced charge-transfer resistance.The rich oxygen vacancies and high structural integrity of the hierarchical yolk-shell spheres enable the as-derived anatase TiO_(2) yolk-shell spheres with a high specific capacity of 280 mAh g^(-1) at 100 mA g^(-1) and 71%of capacity retention after 5000 cycles at 2 A g^(-1).展开更多
Localization,one of the basic phenomena for wave transport,has been demonstrated to be an effective strategy to manipulate electronic,photonic,and acoustic properties of materials.Due to the wave nature of phonons,the...Localization,one of the basic phenomena for wave transport,has been demonstrated to be an effective strategy to manipulate electronic,photonic,and acoustic properties of materials.Due to the wave nature of phonons,the tuning of thermal properties through phonon localization would also be expected,which is beneficial to many applications such as thermoelectrics,electronics,and phononics.With the development of nanotechnology,nanostructures with characteristic length about ten nanometers can give rise to phonon localization,which has attracted considerable attention in recent years.This review aims to summarize recent advances with theoretical,simulative,and experimental studies toward understanding,prediction,and utilization of phonon localization in disordered nanostructures,focuses on the effect of phonon localization on thermal conductivity.Based on previous researches,perspectives regarding further researches to clarify this hecticinvestigated and immature topic and its exact effect on thermal transport are given.展开更多
Highly pathogenic avian influenza(HPAI)viruses such as H5N1,H5N8,H5N6 and H5N2 have been circulating worldwide,posing serious threats to both poultry farming and public health.Notably,the H5N1,H5N6 and H7N9 HPAI virus...Highly pathogenic avian influenza(HPAI)viruses such as H5N1,H5N8,H5N6 and H5N2 have been circulating worldwide,posing serious threats to both poultry farming and public health.Notably,the H5N1,H5N6 and H7N9 HPAI viruses have caused significant mortality in China.Among these,the H5N1 HPAI virus is of great concern due to its impact on global public health(Zhu et al.,2022).Since the first occurrence of H5N1 HPAI virus human infection in 1997,it has manifested a sporadic epidemic pattern in numerous regions across the globe.Thereafter,925 cases of human infection with different clades of H5N1 HPAI viruses have been documented,with a cumulative case fatality rate exceeding 50%,highlighting the severe public health threat posed by the H5N1 HPAI viruses(https://www.cdc.gov/bird-flu/php/technical-report/h5n1-06052024.html?CDC_AAref_Val¼https://www.cdc.gov/flu/avianflu/s potlights/2023-2024/h5n1-technical-report-06052024.htm).Recently,newly H5N1 HPAI viruses were detected on dairy cattle and poultry farms in the United States(US),and spread to herds across fifteen states,resulting in at least nine confirmed human infections during April and September 2024.Subsequently,these viruses also caused many human infections in Asia(e.g.Cambodia and China).This epidemic marks the first confirmed case of the H5N1 HPAI virus being transmitted between humans and other mammalian species,deviating from prior cases involving avian-to-human transmission.展开更多
Breast cancer remains the most prevalent malignancy among women worldwide,with triple-negative breast cancer(TNBC) representing its most aggressive and lethal subtype.TNBC is characterized by high rates of recurrence ...Breast cancer remains the most prevalent malignancy among women worldwide,with triple-negative breast cancer(TNBC) representing its most aggressive and lethal subtype.TNBC is characterized by high rates of recurrence and lung metastasis after surgery,severely impacting patient quality of life.Recent studies highlight the critical role of metabolic reprogramming in driving cancer recurrence,migration,and invasion.While the underlying mechanisms remain complex and not fully elucidated,transcriptomic analyses comparing primary and metastatic breast cancer tissues from TNBC and Luminal patients have identified lysophosphatidylcholine acyltransferase 1(LPCAT1) as a key enzyme upregulated in lung metastases and TNBC.LPCAT1 is strongly associated with poor prognosis due to its activation of the TGFβ signaling pathway.This activation is driven by LPCAT1's ability to increase cellular ATP levels,fostering a high-energy state that stimulates ATPase activity.Consequently,ATP-dependent chromatin remodeling via the BAF complex,which includes double PHD finger 2(DPF2) as a critical subunit,regulates gene transcription essential for tumor progression.Through the LPCAT1-DPF2-TGFBR2 axis,TNBC cells enhance TGFβ signaling,promoting malignant behavior and metastasis.Addressing this,we developed a reduction-responsive nanoparticle platform for the systemic delivery of LPCAT1-targeted si RNA(si LPCAT1),which has shown significant efficacy in suppressing TNBC tumor growth and metastasis.These findings suggest that nanoparticle-mediated si LPCAT1 delivery represents a promising therapeutic strategy for advanced TNBC treatment.展开更多
Through the analysis of the operation of China’s agricultural science technological and development process, the paper summed up the basic experience of agricultural science technological park and operation mode, in ...Through the analysis of the operation of China’s agricultural science technological and development process, the paper summed up the basic experience of agricultural science technological park and operation mode, in order to run at all levels of agricultural science technological park of science and regional some beneficial references for the development of agricultural economy.展开更多
We put forward a two-step route to synthesize vanadium diselenide(VSe_2), a typical transition metal dichalcogenide(TMD). To obtain the VSe_2 film, we first prepare a vanadium film by electron beam evaporation and we ...We put forward a two-step route to synthesize vanadium diselenide(VSe_2), a typical transition metal dichalcogenide(TMD). To obtain the VSe_2 film, we first prepare a vanadium film by electron beam evaporation and we then perform selenization in a vacuum chamber. This method has the advantages of low temperature, is less time-consuming, has a large area, and has a stable performance. At 400?C selenization temperature, we successfully prepare VSe_2 films on both glass and Mo substrates. The prepared VSe_2 has the characteristic of preferential growth along the c-axis, with low transmittance.It is found that the contact between Al and VSe_2/Mo is ohmic contact. Compared to Mo substrate, lower square resistance and higher carrier concentration of the VSe_2/Mo sample reveal that the VSe_2 film may be a potential material for thin film solar cells or other semiconductor devices. The new synthetic strategy that is developed here paves a sustainable way to the application of VSe_2 in photovoltaic devices.展开更多
Recently,the negative differential thermal resistance effect was discovered in a homojunction made of a negative thermal expansion material,which is very promising for realizing macroscopic thermal transistors.Similar...Recently,the negative differential thermal resistance effect was discovered in a homojunction made of a negative thermal expansion material,which is very promising for realizing macroscopic thermal transistors.Similar to the Monte Carlo phonon simulation to deal with grain boundaries,we introduce positive temperature-dependent interface thermal resistance in the modified Lorentz gas model and find negative differential thermal resistance effect.In the homojunction,we reproduce a pair of equivalent negative differential thermal resistance effects in different temperature gradient directions.In the heterojunction,we realize the unidirectional negative differential thermal resistance effect,and it is accompanied by the super thermal rectification effect.Using this new way to achieve high-performance thermal devices is a new direction,and will provide extensive reference and guidance for designing thermal devices.展开更多
Metabolic dysfunction-associated steatotic liver disease(MASLD)is a metabolic disease that can progress to metabolic dysfunction-associated steatohepatitis(MASH),cirrhosis,and cancer.The zonal distribution of biomolec...Metabolic dysfunction-associated steatotic liver disease(MASLD)is a metabolic disease that can progress to metabolic dysfunction-associated steatohepatitis(MASH),cirrhosis,and cancer.The zonal distribution of biomolecules in the liver is implicated in mediat-ing the disease progression.Recently,G-protein-coupled receptor 35(GPR35)has been highlighted to play a role in MASLD,but the precise mechanism is not fully understood,particularly,in a liver-zonal manner.Here,we aimed to identify spatially distributed specific genes and metabolites in different liver zonation that are regulated by GPR35 in MASLD,by combining lipid metabolomics,spatial transcriptomics(ST),and spatial metabolomics(SM).We found that GPR35 influenced lipid accumulation,inflammatory and metabolism-related factors in specific regions,notably affecting the anti-inflammation factor ELF4(E74 like E-twenty six(ETS)tran-scription factor 4),lipid homeostasis key factor CIDEA(cell death-inducing DNA fragmentation factor alpha(DFFA)-like effector A),and the injury response-related genes SAA1/2/3(serum amyloid A1/2/3),thereby impacting MASLD progression.Furthermore,SM elucidated specific metabolite distributions across different liver regions,such as C10H11N4O7P(3ʹ,5ʹ-cyclic inosine monophosphate(3ʹ,5ʹ-IMP))for the central vein,and this metabolite significantly decreased in the liver zones of GPR35-deficient mice during MASLD progression.Taken together,GPR35 regulates hepatocyte damage repair,controls inflammation,and prevents MASLD progression by influencing phospholipid homeostasis and gene expression in a zonal manner.展开更多
To the Editor:Irritable bowel syndrome(IBS)is one of the most common adult functional gastrointestinal disorders(FGIDs),with an estimated global prevalence of 1.5–11.2%.The prevalence rates for anxiety and depressive...To the Editor:Irritable bowel syndrome(IBS)is one of the most common adult functional gastrointestinal disorders(FGIDs),with an estimated global prevalence of 1.5–11.2%.The prevalence rates for anxiety and depressive symptoms and disorders underscore the psychological impact on IBS patients,emphasizing the need for comprehensive diagnostic tools tailored to specific populations.[1]The main diagnostic reference for IBS is the Rome criteria which is now the standard practice for IBS diagnosis worldwide.The most recent iteration is Rome IV[Supplementary Table 1,http://links.lww.com/CM9/C102].Despite its broad acceptance,validation studies demonstrated that the Rome criteria only performed modestly in diagnosing IBS and,in clinical practice,Rome criteria are not commonly used.The overlap between the symptoms of IBS and FGIDs must be considered in the diagnosis of IBS and should be taken into consideration in the development of symptom-based diagnostic tools.展开更多
Platinum-based chemotherapy resistance is a key factor of poor prognosis and recurrence in hepatocellular carcinoma(HCC).Herein,RNAseq analysis revealed that elevated tubulin folding cofactor E(TBCE)expression is asso...Platinum-based chemotherapy resistance is a key factor of poor prognosis and recurrence in hepatocellular carcinoma(HCC).Herein,RNAseq analysis revealed that elevated tubulin folding cofactor E(TBCE)expression is associated with platinum-based chemotherapy resistance.High expression of TBCE contributes to worse prognoses and earlier recurrence among liver cancer patients.Mechanistically,TBCE silencing significantly affects cytoskeleton rearrangement,which in turn increases cisplatin-induced cycle arrest and apoptosis.To develop these findings into potential therapeutic drugs,endosomal pH-responsive nanoparticles(NPs)were developed to simultaneously encapsulate TBCE siRNA and cisplatin(DDP)to reverse this phenomena.NPs(siTBCE+DDP)concurrently silenced TBCE expression,increased cell sensitivity to platinum treatment,and subsequently resulted in superior anti-tumor effects both in vitro and in vivo in orthotopic and patient-derived xenograft(PDX)models.Taken together,NP-mediated delivery and the co-treatment of siTBCE+DDP proved to be effective in reversing chemotherapy resistance of DDP in multiple tumor models.展开更多
Hepatocellular carcinoma(HCC)is a highly heterogeneous tumor,with dynamic equilibrium and complex interplay between its intricate tumor nature and ambient tumor immune microenvironment(TIME).1 Elegant research has ind...Hepatocellular carcinoma(HCC)is a highly heterogeneous tumor,with dynamic equilibrium and complex interplay between its intricate tumor nature and ambient tumor immune microenvironment(TIME).1 Elegant research has indicated that cancer stem cells,a small subset of neoplastic cells confined within dedicated niches,display stem cell-like properties and interact with cells in TIME,thereby imparting an indelible impact on stemness regulation,tumor heterogeneity,and cancer cell plasticity.2 Previous taxonomies solely from the perspective of stemness or TIME may introduce some degree of bias in the comprehension of HCC carcinogenesis,3,4 and thus it is of paramount importance to systematically consider tumor stemness and TIME as a whole to truly portray the biological landscape of HCC.展开更多
Breast cancer is one of the most common malignant tumors with high mortality and poor prognosis in women.There is an urgent need to discover new therapeutic targets for breast cancer metastasis.Herein,we identified th...Breast cancer is one of the most common malignant tumors with high mortality and poor prognosis in women.There is an urgent need to discover new therapeutic targets for breast cancer metastasis.Herein,we identified that Apolipoprotein C1(APOC1)was up-regulated in primary tumor of breast cancer patient that recurrence and metastasis by immunohistochemistry(IHC).Kaplan-Meier Plotter database showed that high levels of APOC1 in breast cancer patients were strongly associated with worse overall survival(OS)and relapse-free survival(RFS).Mechanistically,APOC1 silencing significantly inhibits MAPK/ERK kinase pathway and restrains the NF-κB to decrease the transcription of target genes related to growth and metastasis in vitro.Based on this regulatory mechanism,we developed these findings into potential therapeutic drugs,glutathione(GSH)responsive nanoparticles(NPs)were used for systemic APOC1 siRNA delivery,NPs(siAPOC1)silenced APOC1 expression,and subsequently resulted in positive anti-tumor effects in orthotopic and liver metastasis models in vivo.Taken together,GSH responsive NPmediated siAPOC1 delivery was proved to be effective in regulating growth and metastasis in multiple tumor models.These findings show that APOC1 could be a potential biomarker to predict the prognosis of breast cancer patients and NP-mediated APOC1 silencing could be new strategies for exploration of new treatments for breast cancer metastasis.展开更多
Severe fever with thrombocytopenia syndrome(SFTS),caused by a novel identified bunyavirus SFTS virus(SFTSV),was an emerging viral infectious disease that was firstly reported in China.There are no licensed vaccines an...Severe fever with thrombocytopenia syndrome(SFTS),caused by a novel identified bunyavirus SFTS virus(SFTSV),was an emerging viral infectious disease that was firstly reported in China.There are no licensed vaccines and therapeutics against SFTSV currently.B‐Propiolactone(BPL)inactivated whole virions of SFTSV strain AH12 were prepared as experimental vaccine in different antigen dose with or without Al(OH)3 adjuvant.The experimental SFTS vaccine was a satisfying immunogen,which could efficiently trigger the development of high levels of SFTSV NP‐specific IgG antibodies and neutralizing antibodies against SFTSV Strain HB29 in BALB/c and C57/BL6 mice,and could induce SFTS virus‐specific cellular immune responses to a certain extent.A single dose of vaccine was immunogenically insufficient in BALB/c mice;the second and third dose resulted in significant boost in antibody response.The use of Al(OH)3 adjuvant resulted in higher antibody titers.The mediate‐dose of vaccine could induce as high and equivalent level of antibody titer as that of high‐dose.The experimental SFTS vaccine in mediate‐and high antigen dose with adjuvant resulted in solid protection of C57/BL6 mice against wild‐type SFTSV challenge with markedly accelerated virus clearance from blood and spleen compared with controls.The experimental SFTS vaccine prepared in this study could efficiently elicit virus specific humoral and cellular immune responses in both BALB/c and C57/BL6 mice,and could protect C57/BL6 mice against SFTS virus challenge.These results supplied evidence that inactivated vaccine was a promising vaccine candidate for the prevention of SFTSV infection.展开更多
On-chip manipulation of charged particles using electrophoresis or electroosmosis is widely used for many applications,including optofluidic sensing,bioanalysis and macromolecular data storage.We hereby demonstrate a ...On-chip manipulation of charged particles using electrophoresis or electroosmosis is widely used for many applications,including optofluidic sensing,bioanalysis and macromolecular data storage.We hereby demonstrate a technique for the capture,localization,and release of charged particles and DNA molecules in an aqueous solution using tubular structures enabled by a strain-induced self-rolled-up nanomembrane(S-RuM)platform.Cuffed-in 3D electrodes that are embedded in cylindrical S-RuM structures and biased by a constant DC voltage are used to provide a uniform electrical field inside the microtubular devices.Efficient charged-particle manipulation is achieved at a bias voltage of<2-4 V,which is~3 orders of magnitude lower than the required potential in traditional DC electrophoretic devices.Furthermore,Poisson-Boltzmann multiphysics simulation validates the feasibility and advantage of our microtubular charge manipulation devices over planar and other 3D variations of microfluidic devices.This work lays the foundation for on-chip DNA manipulation for data storage applications.展开更多
基金supported by the Henan medical science and technology research projects(222102310014)
文摘Purpose:To evaluate the feasibility and efficacy of a transmesenteric vein extrahepatic portosystemic shunt(TmEPS)for the treatment of cavernous transformation of the portal vein(CTPV).Materials and methods:The clinical data of 20 patients with CTPV who underwent TmEPS between December 2020and January 2022 at Henan Provincial People’s Hospital were retrospectively collected.The superior mesenteric vein(SMV)trunk was patent or partially occluded in these patients.An extrahepatic portosystemic shunt between the inferior vena cava and the SMV was established using a stent graft through an infraumbilical median longitudinal mini-laparotomy.The technical success,efficacy,and complication rates were evaluated,and the preand postoperative SMV pressures were compared.Patients’clinical outcomes and shunt patency were assessed.Results:TmEPS was successfully performed in 20 patients.The initial puncture success rate of the balloon-assisted puncture technique is 95%.The mean SMV pressure decreased from 29.1±2.9 mmHg to 15.6±3.3 mmHg(p<0.001).All symptoms of portal hypertension resolved.No fatal procedural complications occurred.During the follow-up period,hepatic encephalopathy occurred in two patients.The remaining patients remained asymptomatic.All shunts were patent.Conclusions:TmEPS is a feasible,safe,and effective treatment option for patients with CTPV.
基金supported by the NationallKey R&D Program of China(2019YFB1503200)the NationallScience Foundation(CBET-1803256)+4 种基金the Anhui ProvinciallNaturallScience Foundation(1908085QB52)the CASHIPS Director’s Fund(YZJJ2018QN21)Shijiazhuang University DoctorallScientific Research Startup Fund Project(20BS019)Colleges and universities in Shandong Province science and technology projects(J17KA097)CAS Key Laboratory of Photovoltaic and Energy Conservation,Chinese Academy of Sciences(PECL2018QN006)。
文摘Anatase TiO_(2) is a promising anode material for lithium-ion batteries(LIBs)owing to its low cost and stability.However,the intrinsically kinetic limits seriously hindered its lithium-ion storage capability.Here we present that anatase TiO_(2) with rich oxygen vacancies can enhance its lithium-ion storage performance.We synthesize anatase TiO_(2) with well-retained hierarchical structure by annealing the H_(2)Ti_(5)O_(11)·3H_(2)O yolk-shell spheres precursor in nitrogen atmosphere.EPR and XPS data evidence that the oxygen-deficient environment could generate abundant oxygen vacancies in the as-derived anatase TiO_(2),which leads to improved electron conductivity and reduced charge-transfer resistance.The rich oxygen vacancies and high structural integrity of the hierarchical yolk-shell spheres enable the as-derived anatase TiO_(2) yolk-shell spheres with a high specific capacity of 280 mAh g^(-1) at 100 mA g^(-1) and 71%of capacity retention after 5000 cycles at 2 A g^(-1).
基金Project supported by the National Natural Science Foundation of China(Grant Nos.11975125,11890703,and 21803031)the Natural Science Foundation of the Jiangsu Higher Education Institution of China(Grant No.18KJB150022)+1 种基金Postdoctoral Research Funding Program of Jiangsu,China(Grant No.2020Z163)China Postdoctoral Science Foundation(Grant No.2020M671533)。
文摘Localization,one of the basic phenomena for wave transport,has been demonstrated to be an effective strategy to manipulate electronic,photonic,and acoustic properties of materials.Due to the wave nature of phonons,the tuning of thermal properties through phonon localization would also be expected,which is beneficial to many applications such as thermoelectrics,electronics,and phononics.With the development of nanotechnology,nanostructures with characteristic length about ten nanometers can give rise to phonon localization,which has attracted considerable attention in recent years.This review aims to summarize recent advances with theoretical,simulative,and experimental studies toward understanding,prediction,and utilization of phonon localization in disordered nanostructures,focuses on the effect of phonon localization on thermal conductivity.Based on previous researches,perspectives regarding further researches to clarify this hecticinvestigated and immature topic and its exact effect on thermal transport are given.
文摘Highly pathogenic avian influenza(HPAI)viruses such as H5N1,H5N8,H5N6 and H5N2 have been circulating worldwide,posing serious threats to both poultry farming and public health.Notably,the H5N1,H5N6 and H7N9 HPAI viruses have caused significant mortality in China.Among these,the H5N1 HPAI virus is of great concern due to its impact on global public health(Zhu et al.,2022).Since the first occurrence of H5N1 HPAI virus human infection in 1997,it has manifested a sporadic epidemic pattern in numerous regions across the globe.Thereafter,925 cases of human infection with different clades of H5N1 HPAI viruses have been documented,with a cumulative case fatality rate exceeding 50%,highlighting the severe public health threat posed by the H5N1 HPAI viruses(https://www.cdc.gov/bird-flu/php/technical-report/h5n1-06052024.html?CDC_AAref_Val¼https://www.cdc.gov/flu/avianflu/s potlights/2023-2024/h5n1-technical-report-06052024.htm).Recently,newly H5N1 HPAI viruses were detected on dairy cattle and poultry farms in the United States(US),and spread to herds across fifteen states,resulting in at least nine confirmed human infections during April and September 2024.Subsequently,these viruses also caused many human infections in Asia(e.g.Cambodia and China).This epidemic marks the first confirmed case of the H5N1 HPAI virus being transmitted between humans and other mammalian species,deviating from prior cases involving avian-to-human transmission.
基金supported by the National Natural Science Foundation of China (82173392,81874226,82072930 and 81802700)the grants from Guangdong Science and Technology Department (2024B1515040006,2021A1515111106,2020A1515011298,and 2019A1515011932)+6 种基金Guangdong Province Outstanding Youth Fund Project (2021B1515020066)Guangzhou Province Basic Research Fund (202201020576)Guangzhou Science and Technology Bureau (2024A03J0846,20210303004,and 202103000093)the Natural Science Foundation of Hunan Province (2023JJ50149)111 Project (B20056)the “Three million for Three Years” Project of the High-level Talent Special Funding Scheme of Sun Yat-Sen Memorial HospitalYouth Doctor Program Fund of the Second Affiliated Hospital,Army Medical University (2023YQB003)。
文摘Breast cancer remains the most prevalent malignancy among women worldwide,with triple-negative breast cancer(TNBC) representing its most aggressive and lethal subtype.TNBC is characterized by high rates of recurrence and lung metastasis after surgery,severely impacting patient quality of life.Recent studies highlight the critical role of metabolic reprogramming in driving cancer recurrence,migration,and invasion.While the underlying mechanisms remain complex and not fully elucidated,transcriptomic analyses comparing primary and metastatic breast cancer tissues from TNBC and Luminal patients have identified lysophosphatidylcholine acyltransferase 1(LPCAT1) as a key enzyme upregulated in lung metastases and TNBC.LPCAT1 is strongly associated with poor prognosis due to its activation of the TGFβ signaling pathway.This activation is driven by LPCAT1's ability to increase cellular ATP levels,fostering a high-energy state that stimulates ATPase activity.Consequently,ATP-dependent chromatin remodeling via the BAF complex,which includes double PHD finger 2(DPF2) as a critical subunit,regulates gene transcription essential for tumor progression.Through the LPCAT1-DPF2-TGFBR2 axis,TNBC cells enhance TGFβ signaling,promoting malignant behavior and metastasis.Addressing this,we developed a reduction-responsive nanoparticle platform for the systemic delivery of LPCAT1-targeted si RNA(si LPCAT1),which has shown significant efficacy in suppressing TNBC tumor growth and metastasis.These findings suggest that nanoparticle-mediated si LPCAT1 delivery represents a promising therapeutic strategy for advanced TNBC treatment.
文摘Through the analysis of the operation of China’s agricultural science technological and development process, the paper summed up the basic experience of agricultural science technological park and operation mode, in order to run at all levels of agricultural science technological park of science and regional some beneficial references for the development of agricultural economy.
基金Project supported by the National Natural Science Foundation of China(Grant Nos.51572132,61674082,and 61774089)the National Key R&D Program of China(Grant No.2018YFB1500202)+3 种基金Tianjin Natural Science Foundation of Key Project,China(Grant Nos.18JCZDJC31200 and 16JCZDJC30700)Yang Fan Innovative and Entrepreneurial Research Team Project,China(Grant No.2014YT02N037)111 Project,China(Grant No.B16027)the International Cooperation Base,China(Grant No.2016D01025)
文摘We put forward a two-step route to synthesize vanadium diselenide(VSe_2), a typical transition metal dichalcogenide(TMD). To obtain the VSe_2 film, we first prepare a vanadium film by electron beam evaporation and we then perform selenization in a vacuum chamber. This method has the advantages of low temperature, is less time-consuming, has a large area, and has a stable performance. At 400?C selenization temperature, we successfully prepare VSe_2 films on both glass and Mo substrates. The prepared VSe_2 has the characteristic of preferential growth along the c-axis, with low transmittance.It is found that the contact between Al and VSe_2/Mo is ohmic contact. Compared to Mo substrate, lower square resistance and higher carrier concentration of the VSe_2/Mo sample reveal that the VSe_2 film may be a potential material for thin film solar cells or other semiconductor devices. The new synthetic strategy that is developed here paves a sustainable way to the application of VSe_2 in photovoltaic devices.
基金Supported by the National Natural Science Foundation of China(Grant Nos.11975125 and 21803031)the Postgraduate Research&Practice Innovation Program of Jiangsu Province(Grant No.KYCX201229)the Natural Science Foundation of the Jiangsu Higher Education Institution of China(Grant No.18KJB150022).
文摘Recently,the negative differential thermal resistance effect was discovered in a homojunction made of a negative thermal expansion material,which is very promising for realizing macroscopic thermal transistors.Similar to the Monte Carlo phonon simulation to deal with grain boundaries,we introduce positive temperature-dependent interface thermal resistance in the modified Lorentz gas model and find negative differential thermal resistance effect.In the homojunction,we reproduce a pair of equivalent negative differential thermal resistance effects in different temperature gradient directions.In the heterojunction,we realize the unidirectional negative differential thermal resistance effect,and it is accompanied by the super thermal rectification effect.Using this new way to achieve high-performance thermal devices is a new direction,and will provide extensive reference and guidance for designing thermal devices.
基金supported by the National Key Research and Development Program of China(2022YFA0806503)the National Natural Science Foundation of China(81972625 and 32201217)+3 种基金Liaoning Revitalization Talents Program(XLYC2002035)Liaoning Science and Technology Innovation Funding(20230101-JH2/1013)the Innovation Program of Science and Research from Dalian Institute of Chemical Physics,Chinese Academy of Sciences(DICP I202129 and DICP I202209)the Science and Technology Innovation Fund(Youth Science and Technology Star)of Dalian(2021RQ009 and 2023RQ040).
文摘Metabolic dysfunction-associated steatotic liver disease(MASLD)is a metabolic disease that can progress to metabolic dysfunction-associated steatohepatitis(MASH),cirrhosis,and cancer.The zonal distribution of biomolecules in the liver is implicated in mediat-ing the disease progression.Recently,G-protein-coupled receptor 35(GPR35)has been highlighted to play a role in MASLD,but the precise mechanism is not fully understood,particularly,in a liver-zonal manner.Here,we aimed to identify spatially distributed specific genes and metabolites in different liver zonation that are regulated by GPR35 in MASLD,by combining lipid metabolomics,spatial transcriptomics(ST),and spatial metabolomics(SM).We found that GPR35 influenced lipid accumulation,inflammatory and metabolism-related factors in specific regions,notably affecting the anti-inflammation factor ELF4(E74 like E-twenty six(ETS)tran-scription factor 4),lipid homeostasis key factor CIDEA(cell death-inducing DNA fragmentation factor alpha(DFFA)-like effector A),and the injury response-related genes SAA1/2/3(serum amyloid A1/2/3),thereby impacting MASLD progression.Furthermore,SM elucidated specific metabolite distributions across different liver regions,such as C10H11N4O7P(3ʹ,5ʹ-cyclic inosine monophosphate(3ʹ,5ʹ-IMP))for the central vein,and this metabolite significantly decreased in the liver zones of GPR35-deficient mice during MASLD progression.Taken together,GPR35 regulates hepatocyte damage repair,controls inflammation,and prevents MASLD progression by influencing phospholipid homeostasis and gene expression in a zonal manner.
文摘To the Editor:Irritable bowel syndrome(IBS)is one of the most common adult functional gastrointestinal disorders(FGIDs),with an estimated global prevalence of 1.5–11.2%.The prevalence rates for anxiety and depressive symptoms and disorders underscore the psychological impact on IBS patients,emphasizing the need for comprehensive diagnostic tools tailored to specific populations.[1]The main diagnostic reference for IBS is the Rome criteria which is now the standard practice for IBS diagnosis worldwide.The most recent iteration is Rome IV[Supplementary Table 1,http://links.lww.com/CM9/C102].Despite its broad acceptance,validation studies demonstrated that the Rome criteria only performed modestly in diagnosing IBS and,in clinical practice,Rome criteria are not commonly used.The overlap between the symptoms of IBS and FGIDs must be considered in the diagnosis of IBS and should be taken into consideration in the development of symptom-based diagnostic tools.
基金supported by grants from the National Natural Science Foundation of China (81874226 and 81803020)the “Three Million for Three Years” Project of the High-Level Talent Special Funding Scheme of Sun Yat-sen Memorial Hospitalthe Guangdong Natural Science Foundation (2016A030313834and 2017A030313871,China)
文摘Platinum-based chemotherapy resistance is a key factor of poor prognosis and recurrence in hepatocellular carcinoma(HCC).Herein,RNAseq analysis revealed that elevated tubulin folding cofactor E(TBCE)expression is associated with platinum-based chemotherapy resistance.High expression of TBCE contributes to worse prognoses and earlier recurrence among liver cancer patients.Mechanistically,TBCE silencing significantly affects cytoskeleton rearrangement,which in turn increases cisplatin-induced cycle arrest and apoptosis.To develop these findings into potential therapeutic drugs,endosomal pH-responsive nanoparticles(NPs)were developed to simultaneously encapsulate TBCE siRNA and cisplatin(DDP)to reverse this phenomena.NPs(siTBCE+DDP)concurrently silenced TBCE expression,increased cell sensitivity to platinum treatment,and subsequently resulted in superior anti-tumor effects both in vitro and in vivo in orthotopic and patient-derived xenograft(PDX)models.Taken together,NP-mediated delivery and the co-treatment of siTBCE+DDP proved to be effective in reversing chemotherapy resistance of DDP in multiple tumor models.
文摘Hepatocellular carcinoma(HCC)is a highly heterogeneous tumor,with dynamic equilibrium and complex interplay between its intricate tumor nature and ambient tumor immune microenvironment(TIME).1 Elegant research has indicated that cancer stem cells,a small subset of neoplastic cells confined within dedicated niches,display stem cell-like properties and interact with cells in TIME,thereby imparting an indelible impact on stemness regulation,tumor heterogeneity,and cancer cell plasticity.2 Previous taxonomies solely from the perspective of stemness or TIME may introduce some degree of bias in the comprehension of HCC carcinogenesis,3,4 and thus it is of paramount importance to systematically consider tumor stemness and TIME as a whole to truly portray the biological landscape of HCC.
基金supported by the National Natural Science Foundation of China(81570764)Guangzhou Science and Technology Project(201807010069)+2 种基金Shenzhen Science and Technology Project(JCYJ20190807154205627)Guangdong Natural Science Fund(2020A1515010365)Guangdong Provincial Key Laboratory of Precision Medicine and Clinical Translation Research of Hakka Population(2018B030322003KF01).
文摘Breast cancer is one of the most common malignant tumors with high mortality and poor prognosis in women.There is an urgent need to discover new therapeutic targets for breast cancer metastasis.Herein,we identified that Apolipoprotein C1(APOC1)was up-regulated in primary tumor of breast cancer patient that recurrence and metastasis by immunohistochemistry(IHC).Kaplan-Meier Plotter database showed that high levels of APOC1 in breast cancer patients were strongly associated with worse overall survival(OS)and relapse-free survival(RFS).Mechanistically,APOC1 silencing significantly inhibits MAPK/ERK kinase pathway and restrains the NF-κB to decrease the transcription of target genes related to growth and metastasis in vitro.Based on this regulatory mechanism,we developed these findings into potential therapeutic drugs,glutathione(GSH)responsive nanoparticles(NPs)were used for systemic APOC1 siRNA delivery,NPs(siAPOC1)silenced APOC1 expression,and subsequently resulted in positive anti-tumor effects in orthotopic and liver metastasis models in vivo.Taken together,GSH responsive NPmediated siAPOC1 delivery was proved to be effective in regulating growth and metastasis in multiple tumor models.These findings show that APOC1 could be a potential biomarker to predict the prognosis of breast cancer patients and NP-mediated APOC1 silencing could be new strategies for exploration of new treatments for breast cancer metastasis.
基金This study was supported by the National Major Science and Technology Project of China(2018ZX10711001 and 2013ZX09102029)The funders had no role in study design,data collection and analysis,decision to publish,or preparation of the manuscript.
文摘Severe fever with thrombocytopenia syndrome(SFTS),caused by a novel identified bunyavirus SFTS virus(SFTSV),was an emerging viral infectious disease that was firstly reported in China.There are no licensed vaccines and therapeutics against SFTSV currently.B‐Propiolactone(BPL)inactivated whole virions of SFTSV strain AH12 were prepared as experimental vaccine in different antigen dose with or without Al(OH)3 adjuvant.The experimental SFTS vaccine was a satisfying immunogen,which could efficiently trigger the development of high levels of SFTSV NP‐specific IgG antibodies and neutralizing antibodies against SFTSV Strain HB29 in BALB/c and C57/BL6 mice,and could induce SFTS virus‐specific cellular immune responses to a certain extent.A single dose of vaccine was immunogenically insufficient in BALB/c mice;the second and third dose resulted in significant boost in antibody response.The use of Al(OH)3 adjuvant resulted in higher antibody titers.The mediate‐dose of vaccine could induce as high and equivalent level of antibody titer as that of high‐dose.The experimental SFTS vaccine in mediate‐and high antigen dose with adjuvant resulted in solid protection of C57/BL6 mice against wild‐type SFTSV challenge with markedly accelerated virus clearance from blood and spleen compared with controls.The experimental SFTS vaccine prepared in this study could efficiently elicit virus specific humoral and cellular immune responses in both BALB/c and C57/BL6 mice,and could protect C57/BL6 mice against SFTS virus challenge.These results supplied evidence that inactivated vaccine was a promising vaccine candidate for the prevention of SFTSV infection.
基金This work was supported in part by the SemiSynBio grant:SRC 2018-SB-2839NSF CCF 18-07526。
文摘On-chip manipulation of charged particles using electrophoresis or electroosmosis is widely used for many applications,including optofluidic sensing,bioanalysis and macromolecular data storage.We hereby demonstrate a technique for the capture,localization,and release of charged particles and DNA molecules in an aqueous solution using tubular structures enabled by a strain-induced self-rolled-up nanomembrane(S-RuM)platform.Cuffed-in 3D electrodes that are embedded in cylindrical S-RuM structures and biased by a constant DC voltage are used to provide a uniform electrical field inside the microtubular devices.Efficient charged-particle manipulation is achieved at a bias voltage of<2-4 V,which is~3 orders of magnitude lower than the required potential in traditional DC electrophoretic devices.Furthermore,Poisson-Boltzmann multiphysics simulation validates the feasibility and advantage of our microtubular charge manipulation devices over planar and other 3D variations of microfluidic devices.This work lays the foundation for on-chip DNA manipulation for data storage applications.
