The liquid-solid transitions of （Co2Si＋CoSi） and （CoSi＋CoSi2） eutectic alloys were realized in drop tube and the rapid eutectic growth mechanism of intermetallic compounds was examined. The experimental and calc...The liquid-solid transitions of （Co2Si＋CoSi） and （CoSi＋CoSi2） eutectic alloys were realized in drop tube and the rapid eutectic growth mechanism of intermetallic compounds was examined. The experimental and calculated results indicate that with increasing Co content, the intermetallic compound prefers nucleating primarily. The eutectic microstructures experience the transitions of ＇lamellar-anomalous-divorced＇ eutectic with undercooling. In undercooled state, the growth of CoSi intermetallic compound always lags behind others, and no matter how large the undercooling is, this intermetallic compound grows under the solutal diffusion control The calculated coupled zone demonstrates that （Co2Si＋CoSi） eutectic can form within certain undercooling regime, when the composition is in the range from 23.6% to 25.4% Si. And the calculated coupled zone of （CoSi＋CoSi2） covers a composition range from 40.8% to 43.8% Si.展开更多
Preeclampsia(PE)is a serious pregnancy-related disorder characterized by dysregulated glycolysis and aberrant histone lactylation in the placenta.In this study,we developed folic acid(FA)-modified lipid nanoparticles(...Preeclampsia(PE)is a serious pregnancy-related disorder characterized by dysregulated glycolysis and aberrant histone lactylation in the placenta.In this study,we developed folic acid(FA)-modified lipid nanoparticles(FA-LNPs)encapsulating small interfering RNA targeting pyruvate kinase M(si-PKM),termed FA-LNP@si-PKM,to specifically modulate the molecular drivers of PE.Integrated transcriptomic and proteomic profiling identified PKM as a critical regulator in PE pathogenesis,associated with excessive lactate production and increased histone H3 lysine 18(H3K18)lactylation.Further mechanistic studies revealed that the histone acetyltransferase lysine acetyltransferase 7(KAT7)plays a pivotal role in mediating this lactylation process.In vitro silencing of PKM significantly reduced lactate accumulation,suppressed H3K18 lactylation,and attenuated pro-inflammatory cytokine production.Conversely,KAT7 overexpression abrogated these effects,highlighting its essential role in the PKM-lactate-H3K18la axis.In vivo,systemic administration of FA-LNP@si-PKM in an L-NAME-induced murine model of PE led to marked improvements,including reduced systolic blood pressure and proteinuria,diminished placental H3K18la levels,and lower expression of inflammatory markers IL-6 and TNF-α.These findings underscore the therapeutic potential of targeting the KAT7-H3K18la signaling axis using FA-LNP-mediated siRNA delivery.This nanotechnology-based approach offers a promising strategy for addressing the molecular etiology of PE and enhancing maternal and fetal outcomes.展开更多
Rapid eutectic growth of Sb-24%Cu alloy is realized in the drop tube during the free fall under the con-tainerless condition. Based on the analysis of crystal nuclea-tion and eutectic growth in the free fall condition...Rapid eutectic growth of Sb-24%Cu alloy is realized in the drop tube during the free fall under the con-tainerless condition. Based on the analysis of crystal nuclea-tion and eutectic growth in the free fall condition, it is indicated that, with the increase of undercooling, microstruc-tural transition of Sb-24%Cu eutectic alloy proceeds from lamellar to anomalous eutectic structure. Undercoolings of 0 -154 K have been obtained in experiment. The maximum undercooling exceeds to 0.19TE. Calculated results exhibit that Cu2Sb compound is the primary nucleation phase, and that the primary Sb dendrite will grow more rapidly than the eutectic structure when undercooling is larger than 40 K. The eutectic coupled zone around Sb-24%Cu eutectic alloy leads strongly to the Cu-rich side and covers a composition range from 23.0% to 32.7%Sb.展开更多
Droplets of Cu-20%Sb hypoeutectic alloy has been rapidly solidified in drop tube within the containerless condition. With the decrease of droplet diameter, undercooling increases and the microstructures of primary cop...Droplets of Cu-20%Sb hypoeutectic alloy has been rapidly solidified in drop tube within the containerless condition. With the decrease of droplet diameter, undercooling increases and the microstructures of primary copper dendrite refines. Undercooling up to 207 K (0.17 TL) is obtained in experiment. Theoretic analysis indicated that, because of the broad temperature range of solidification, the rapid growth of primary copper dendrite is controlled by the solutal diffusion. Judging from the calculation of T0 curve in the phase diagram, it is shown that the critical undercooling of segregationless solidification is △T0=474 K. At the maximum undercooling of 207 K, the growth velocity of primary copper phase exceeds to 37 mm/s, and the distinct solute trapping occurs.展开更多
基金supported by the National Natural Science Foundations of China (Grant Nos. 50871090, 50971104 and 50901060)the NPU Foundations for Fundamental Research (JC20110279)the financial support from the National Aerospace Science Foundation of China (Grant Nos. 2008ZF53052 and 2010ZF53059)
文摘The liquid-solid transitions of （Co2Si＋CoSi） and （CoSi＋CoSi2） eutectic alloys were realized in drop tube and the rapid eutectic growth mechanism of intermetallic compounds was examined. The experimental and calculated results indicate that with increasing Co content, the intermetallic compound prefers nucleating primarily. The eutectic microstructures experience the transitions of ＇lamellar-anomalous-divorced＇ eutectic with undercooling. In undercooled state, the growth of CoSi intermetallic compound always lags behind others, and no matter how large the undercooling is, this intermetallic compound grows under the solutal diffusion control The calculated coupled zone demonstrates that （Co2Si＋CoSi） eutectic can form within certain undercooling regime, when the composition is in the range from 23.6% to 25.4% Si. And the calculated coupled zone of （CoSi＋CoSi2） covers a composition range from 40.8% to 43.8% Si.
基金supported by the 2025 National Health Commission Scientific Research Fund-Major Science and Technology Plan Project of Zhejiang Province(No.WKJ-ZJ-2533).
文摘Preeclampsia(PE)is a serious pregnancy-related disorder characterized by dysregulated glycolysis and aberrant histone lactylation in the placenta.In this study,we developed folic acid(FA)-modified lipid nanoparticles(FA-LNPs)encapsulating small interfering RNA targeting pyruvate kinase M(si-PKM),termed FA-LNP@si-PKM,to specifically modulate the molecular drivers of PE.Integrated transcriptomic and proteomic profiling identified PKM as a critical regulator in PE pathogenesis,associated with excessive lactate production and increased histone H3 lysine 18(H3K18)lactylation.Further mechanistic studies revealed that the histone acetyltransferase lysine acetyltransferase 7(KAT7)plays a pivotal role in mediating this lactylation process.In vitro silencing of PKM significantly reduced lactate accumulation,suppressed H3K18 lactylation,and attenuated pro-inflammatory cytokine production.Conversely,KAT7 overexpression abrogated these effects,highlighting its essential role in the PKM-lactate-H3K18la axis.In vivo,systemic administration of FA-LNP@si-PKM in an L-NAME-induced murine model of PE led to marked improvements,including reduced systolic blood pressure and proteinuria,diminished placental H3K18la levels,and lower expression of inflammatory markers IL-6 and TNF-α.These findings underscore the therapeutic potential of targeting the KAT7-H3K18la signaling axis using FA-LNP-mediated siRNA delivery.This nanotechnology-based approach offers a promising strategy for addressing the molecular etiology of PE and enhancing maternal and fetal outcomes.
基金This work was supported by the National Natural Science Foundation of China (Grant Nos. 59901009, 59876016 and 59871040) Huo Yingdong Education Foundation (Grant No. 71044).
文摘Rapid eutectic growth of Sb-24%Cu alloy is realized in the drop tube during the free fall under the con-tainerless condition. Based on the analysis of crystal nuclea-tion and eutectic growth in the free fall condition, it is indicated that, with the increase of undercooling, microstruc-tural transition of Sb-24%Cu eutectic alloy proceeds from lamellar to anomalous eutectic structure. Undercoolings of 0 -154 K have been obtained in experiment. The maximum undercooling exceeds to 0.19TE. Calculated results exhibit that Cu2Sb compound is the primary nucleation phase, and that the primary Sb dendrite will grow more rapidly than the eutectic structure when undercooling is larger than 40 K. The eutectic coupled zone around Sb-24%Cu eutectic alloy leads strongly to the Cu-rich side and covers a composition range from 23.0% to 32.7%Sb.
基金This work was supported by the National Natural Science Foundation of China (Grant Nos. 59901009 and 50101010) Huo Yingdong Education Foundation (Grant No. 71044).
文摘Droplets of Cu-20%Sb hypoeutectic alloy has been rapidly solidified in drop tube within the containerless condition. With the decrease of droplet diameter, undercooling increases and the microstructures of primary copper dendrite refines. Undercooling up to 207 K (0.17 TL) is obtained in experiment. Theoretic analysis indicated that, because of the broad temperature range of solidification, the rapid growth of primary copper dendrite is controlled by the solutal diffusion. Judging from the calculation of T0 curve in the phase diagram, it is shown that the critical undercooling of segregationless solidification is △T0=474 K. At the maximum undercooling of 207 K, the growth velocity of primary copper phase exceeds to 37 mm/s, and the distinct solute trapping occurs.
