For full use of Skipjack tuna(Katsuwonus pelamis)canning by-products,gelatins(STG)were extracted from tuna skins using acid(STG-A),enzyme(STG-E)and hot water(STG-H)methods with yields of 65.30±1.56%,39.83±1....For full use of Skipjack tuna(Katsuwonus pelamis)canning by-products,gelatins(STG)were extracted from tuna skins using acid(STG-A),enzyme(STG-E)and hot water(STG-H)methods with yields of 65.30±1.56%,39.83±1.61%,and 50.97±1.44%,respectively.Due to the high imino acid contents(228,216,and 213 residues/1000 residues for STG-A,STG-E,and STG-H,respectively),STG-A showed the highest transparency and gel strength properties.Amino acid analysis,sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE),ultraviolet(UV)and Fourier transform infrared spectroscopy(FTIR)analysis confirmed that STG-A,STG-E,and STG-H kept the main structure of type I collagen,but enzyme and hot water extraction methods showed much stronger hydrolysis ability onαandβchains.Moreover,neutrase hydrolysate(STG-AH)of STG-A showed the highest 1,1-diphenyl-2-picrylhydrazyl radical(DPPH•)scavenging activity and the fraction STG-AH-І(<3.5 kDa)from STG-AH prepared by ultrafiltration could significantly protect human skin fibroblasts(HSFBs)against ultraviolet-A(UVA)injury.Furthermore,nineteen peptides with high antioxidant activity were purified from STG-AH-Іand identified.These results suggested that STG-AH and its derived antioxidant peptides could serve as potential ingredients applied in health benefiting products for preventing UVA injury.展开更多
The timing and location of chicken(Gallus gallus domesticus)domestication have remained controversial for over a century[1].Several researchers have proposed China as one of the early centers for chicken domestication...The timing and location of chicken(Gallus gallus domesticus)domestication have remained controversial for over a century[1].Several researchers have proposed China as one of the early centers for chicken domestication[2].This is based on a few archaeological‘‘chicken"remains discovered in China(Table S1).Recently,one investigation detected mitochondrial DNA(mt DNA)haplotypes展开更多
基金funded by the National Natural Science Foundation of China(No.82073764)the Ten-thousand Talents Plan of Zhejiang Province(No.2019R52026).
文摘For full use of Skipjack tuna(Katsuwonus pelamis)canning by-products,gelatins(STG)were extracted from tuna skins using acid(STG-A),enzyme(STG-E)and hot water(STG-H)methods with yields of 65.30±1.56%,39.83±1.61%,and 50.97±1.44%,respectively.Due to the high imino acid contents(228,216,and 213 residues/1000 residues for STG-A,STG-E,and STG-H,respectively),STG-A showed the highest transparency and gel strength properties.Amino acid analysis,sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE),ultraviolet(UV)and Fourier transform infrared spectroscopy(FTIR)analysis confirmed that STG-A,STG-E,and STG-H kept the main structure of type I collagen,but enzyme and hot water extraction methods showed much stronger hydrolysis ability onαandβchains.Moreover,neutrase hydrolysate(STG-AH)of STG-A showed the highest 1,1-diphenyl-2-picrylhydrazyl radical(DPPH•)scavenging activity and the fraction STG-AH-І(<3.5 kDa)from STG-AH prepared by ultrafiltration could significantly protect human skin fibroblasts(HSFBs)against ultraviolet-A(UVA)injury.Furthermore,nineteen peptides with high antioxidant activity were purified from STG-AH-Іand identified.These results suggested that STG-AH and its derived antioxidant peptides could serve as potential ingredients applied in health benefiting products for preventing UVA injury.
基金supported by Guangdong Natural Science Foundation(2014A030307018)Science&Technology Planning Project of Guangdong Province(2015A020208020,2016A030303068)+2 种基金Southeast Asia Biodiversity Research Institute,Chinese Academy of Sciences(Y4ZK111B01:2015CASEABRI002)the Animal Branch of the Germplasm Bank of Wild Species,Chinese Academy of SciencesThe Institute of Animals for Scientific Purpose Development(IAD)provided permission(U1-01205-2558)in Thailand
文摘The timing and location of chicken(Gallus gallus domesticus)domestication have remained controversial for over a century[1].Several researchers have proposed China as one of the early centers for chicken domestication[2].This is based on a few archaeological‘‘chicken"remains discovered in China(Table S1).Recently,one investigation detected mitochondrial DNA(mt DNA)haplotypes
