Comprehensive Summary The advancement of bioorthogonal cleavage reactions(BCRs)has expanded the scope of the bioorthogonal chemistry toolkit,leading to a diverse array of innovative biological applications.These inclu...Comprehensive Summary The advancement of bioorthogonal cleavage reactions(BCRs)has expanded the scope of the bioorthogonal chemistry toolkit,leading to a diverse array of innovative biological applications.These include but are not limited to precise spatial and temporal activation of intracellular probes,prodrugs,proteins,glycans,and nucleic acids.Herein,we summarize recent efforts by our group to develop BCRs for manipulating functional molecules in living species to meet various needs,along with future perspectives in this exciting field.展开更多
The 2022 Nobel Prize in Chemistry was awarded to Carolyn R.Bertozzi,Morten Meldal andK.Barry Sharpless for their development of click reaction and bioorthogonal chemistry.Our understanding of life sciences has been fu...The 2022 Nobel Prize in Chemistry was awarded to Carolyn R.Bertozzi,Morten Meldal andK.Barry Sharpless for their development of click reaction and bioorthogonal chemistry.Our understanding of life sciences has been fundamentally accelerated by such novel chemistries,and to some extent,revolutionized by the emergence of new chemical tools benefited from these cutting-edge interdisciplinary explorations.In turn,the high demands of more powerful chemical tools for the sophisticated living systems have further promoted the development of new chemistry with newfunctionalities and applications.展开更多
Enzyme-and catalyst-generated reactive species have been leveraged in the past decade to covalently label biomolecules within a short range of a defined site or space inside cells or at the cell–cell interface.Due to...Enzyme-and catalyst-generated reactive species have been leveraged in the past decade to covalently label biomolecules within a short range of a defined site or space inside cells or at the cell–cell interface.Due to their high spatial resolution,such proximity labeling strategies have been coupled with various bioanalytical techniques for dissecting dynamic and complex biological processes.Here,we review the development of enzyme-and catalyst-triggered proximity chemistry and their applications to identifying protein interaction networks as well as cell–cell communications in living systems.展开更多
基金funding from the Ministry of Science and Technology of China(2023YFA1506501 and 2022YFA1304700 to P.R.C.)the Beijing Municipal Science and Technology Commission Project(Z200010)+1 种基金the National Natural Science Foundation of China(21937001,22137001,91753000 to P.R.C.,and 22222701,92253301,and 22077004 to X.F.)New Cornerstone Science Foundation through the New Cornerstone Investigator Program(P.R.C.).
文摘Comprehensive Summary The advancement of bioorthogonal cleavage reactions(BCRs)has expanded the scope of the bioorthogonal chemistry toolkit,leading to a diverse array of innovative biological applications.These include but are not limited to precise spatial and temporal activation of intracellular probes,prodrugs,proteins,glycans,and nucleic acids.Herein,we summarize recent efforts by our group to develop BCRs for manipulating functional molecules in living species to meet various needs,along with future perspectives in this exciting field.
基金supported by the National Natural Science Foundation of China(21937001,22137001,22222701,22077004)the Ministry of Science and Technology(2019YFA0904201)the Beijing Natural Science Foundation(Z200010)。
文摘The 2022 Nobel Prize in Chemistry was awarded to Carolyn R.Bertozzi,Morten Meldal andK.Barry Sharpless for their development of click reaction and bioorthogonal chemistry.Our understanding of life sciences has been fundamentally accelerated by such novel chemistries,and to some extent,revolutionized by the emergence of new chemical tools benefited from these cutting-edge interdisciplinary explorations.In turn,the high demands of more powerful chemical tools for the sophisticated living systems have further promoted the development of new chemistry with newfunctionalities and applications.
基金funding from the National Natural Science Foundation of China(grant nos.21937001,22222701,22137001,91957101,and 22077004)the Ministry of Science and Technology of China(grant nos.2022YFA1304700,2019YFA0904201,and 2021YFA1302603)Beijing Natural Science Foundation(grant no.Z200010).
文摘Enzyme-and catalyst-generated reactive species have been leveraged in the past decade to covalently label biomolecules within a short range of a defined site or space inside cells or at the cell–cell interface.Due to their high spatial resolution,such proximity labeling strategies have been coupled with various bioanalytical techniques for dissecting dynamic and complex biological processes.Here,we review the development of enzyme-and catalyst-triggered proximity chemistry and their applications to identifying protein interaction networks as well as cell–cell communications in living systems.
