BRASSINAZOLE-RESISTANT 1 family proteins(BZRs)are central transcription factors that govern brassinosteroid(BR)-regulated gene expression and plant growth.However,it is unclear whether there exists a BZR-independent p...BRASSINAZOLE-RESISTANT 1 family proteins(BZRs)are central transcription factors that govern brassinosteroid(BR)-regulated gene expression and plant growth.However,it is unclear whether there exists a BZR-independent pathway that mediates BR signaling.In this study,we found that disruption of all BZRs in Arabidopsis generated a hextuple mutant(bzr-h)displaying vegetative growth phenotypes that were almost identical to those of the null mutant of three BR receptors,bri1brl1brl3(bri-t).By RNA sequencing,we found that global gene expression in bzr-h was unaffected by 2 h of BR treatment.The anthers of bzr-h plants were loculeless,but a similar phenotype was not observed in bri-t,suggesting that BZRs have a BR signaling-independent regulatory role in anther development.By real-time PCR and in situ hybridization,we found that the expression of SPOROCYTELESS(SPL),which encodes a transcription factor essential for anther locule development,was barely detectable in bzr-h,suggesting that BZRs regulate locule development by affecting SPL expression.Our findings reveal that BZRs are indispensable transcription factors required for both BR signaling and anther locule development,providing new insight into the molecular mechanisms underlying the microsporogenesis in Arabidopsis.展开更多
Thermomorphogenesis and the heat shock(HS)response are distinct thermal responses in plants that are regulated by PHYTOCHROME-INTERACTING FACTOR 4(PIF4)and HEAT SHOCK FACTOR A1s(HSFA1s),respectively.Little is known ab...Thermomorphogenesis and the heat shock(HS)response are distinct thermal responses in plants that are regulated by PHYTOCHROME-INTERACTING FACTOR 4(PIF4)and HEAT SHOCK FACTOR A1s(HSFA1s),respectively.Little is known about whether these responses are interconnected and whether they are activated by similar mechanisms.An analysis of transcriptome dynamics in response to warm temperature(28℃)treatment revealed that 30 min of exposure activated the expression of a subset of HSFA1 target genes in Arabidopsis thaliana.Meanwhile,a loss-of-function HSFA1 quadruple mutant(hsfa1-cq)was insensitive to warm temperature-induced hypocotyl growth.In hsfa1-cq plants grown at 28℃,the protein and transcript levels of PIF4 were greatly reduced,and the circadian rhythm of many thermomorphogenesis-related genes(including PIF4)was disturbed.Additionally,the nuclear localization of HSFA1s and the binding of HSFA1d to the PIF4 promoter increased following warm temperature exposure,whereas PIF4 overexpression in hsfa1-cq partially rescued the altered warm temperature-induced hypocotyl growth of the mutant.Taken together,these results suggest that HSFA1s are required for PIF4 accumulation at a warm temperature,and they establish a central role for HSFA1s in regulating both thermomorphogenesis and HS responses in Arabidopsis.展开更多
Facile mass transport channel and accessible active sites are crucial for binder-free air electrode catalysts in rechargeable flexible zinc-air battery(ZAB).Herein,a ZnS/NH3 dual-assisted pyrolysis strategy is propose...Facile mass transport channel and accessible active sites are crucial for binder-free air electrode catalysts in rechargeable flexible zinc-air battery(ZAB).Herein,a ZnS/NH3 dual-assisted pyrolysis strategy is proposed to prepare N/S-doped hierarchical porous bamboo carbon cloth(HP-NS-BCC)as binder-free air electrode catalyst for ZAB.BCC fabric with abundant micropores is firstly used as flexible carbon support to facilitate the heteroatom-doping and construct the hierarchical porous structure.ZnS nanospheres and NH3 activization together facilitate the electronic modulation of carbon matrix by N/S-doping and optimize the macro/meso/micropores structure of carbon fibers.Benefiting from the highly-exposed N/S-induced sites with enhanced intrinsic activity,the optimized mass transport of biocarbon fibers,as well as the ultra-large specific surface area of 2436.1 m^(2)·g^(-1),the resultant HP-NS-BCC catalyst exhibits improved kinetics for oxygen reduction/evolution reaction.When applied to rechargeable aqueous ZABs,it achieves a significant peak power density of 249.1 mW·cm^(-2).As binder-free air electrode catalyst,the flexible ZAB also displays stable cycling over 500 cycles with a minimal voltage gap of 0.42 V,showcasing promising applications in flexible electronic devices.展开更多
基金grants from the National Natural Science Foundation of China(91417313 to W.T.)the Department of Education of Hebei Province(LJRC015 to W.T.)the "One Hundred Talents Project"of Hebei Province(E2013100004 to Y.S.).
文摘BRASSINAZOLE-RESISTANT 1 family proteins(BZRs)are central transcription factors that govern brassinosteroid(BR)-regulated gene expression and plant growth.However,it is unclear whether there exists a BZR-independent pathway that mediates BR signaling.In this study,we found that disruption of all BZRs in Arabidopsis generated a hextuple mutant(bzr-h)displaying vegetative growth phenotypes that were almost identical to those of the null mutant of three BR receptors,bri1brl1brl3(bri-t).By RNA sequencing,we found that global gene expression in bzr-h was unaffected by 2 h of BR treatment.The anthers of bzr-h plants were loculeless,but a similar phenotype was not observed in bri-t,suggesting that BZRs have a BR signaling-independent regulatory role in anther development.By real-time PCR and in situ hybridization,we found that the expression of SPOROCYTELESS(SPL),which encodes a transcription factor essential for anther locule development,was barely detectable in bzr-h,suggesting that BZRs regulate locule development by affecting SPL expression.Our findings reveal that BZRs are indispensable transcription factors required for both BR signaling and anther locule development,providing new insight into the molecular mechanisms underlying the microsporogenesis in Arabidopsis.
基金supported by grants from the National Natural Science Foundation of China(32070293)the Natural Science Foundation of Hebei Province(C2020205028,C2021205013,C2021205009)High-Level Talent Team Construction Project of Hebei Province(225A2902D).
文摘Thermomorphogenesis and the heat shock(HS)response are distinct thermal responses in plants that are regulated by PHYTOCHROME-INTERACTING FACTOR 4(PIF4)and HEAT SHOCK FACTOR A1s(HSFA1s),respectively.Little is known about whether these responses are interconnected and whether they are activated by similar mechanisms.An analysis of transcriptome dynamics in response to warm temperature(28℃)treatment revealed that 30 min of exposure activated the expression of a subset of HSFA1 target genes in Arabidopsis thaliana.Meanwhile,a loss-of-function HSFA1 quadruple mutant(hsfa1-cq)was insensitive to warm temperature-induced hypocotyl growth.In hsfa1-cq plants grown at 28℃,the protein and transcript levels of PIF4 were greatly reduced,and the circadian rhythm of many thermomorphogenesis-related genes(including PIF4)was disturbed.Additionally,the nuclear localization of HSFA1s and the binding of HSFA1d to the PIF4 promoter increased following warm temperature exposure,whereas PIF4 overexpression in hsfa1-cq partially rescued the altered warm temperature-induced hypocotyl growth of the mutant.Taken together,these results suggest that HSFA1s are required for PIF4 accumulation at a warm temperature,and they establish a central role for HSFA1s in regulating both thermomorphogenesis and HS responses in Arabidopsis.
基金supported by the National Natural Science Foundation of China(Grant Nos.52102260,22379056,52202243)the project funded by China Postdoctoral Science Foundation(Grant Nos.2022M711545,2022M711371)+1 种基金Research and Practice Innovation Plan of Postgraduate Training Innovation Project in Jiangsu Province(SJCX23_2156)Shanghai Frontiers Research Center of the Hadal Biosphere,SciTech Funding by CSPFTZ Lingang Special Area Marine Biomedical Innovation Platform.
文摘Facile mass transport channel and accessible active sites are crucial for binder-free air electrode catalysts in rechargeable flexible zinc-air battery(ZAB).Herein,a ZnS/NH3 dual-assisted pyrolysis strategy is proposed to prepare N/S-doped hierarchical porous bamboo carbon cloth(HP-NS-BCC)as binder-free air electrode catalyst for ZAB.BCC fabric with abundant micropores is firstly used as flexible carbon support to facilitate the heteroatom-doping and construct the hierarchical porous structure.ZnS nanospheres and NH3 activization together facilitate the electronic modulation of carbon matrix by N/S-doping and optimize the macro/meso/micropores structure of carbon fibers.Benefiting from the highly-exposed N/S-induced sites with enhanced intrinsic activity,the optimized mass transport of biocarbon fibers,as well as the ultra-large specific surface area of 2436.1 m^(2)·g^(-1),the resultant HP-NS-BCC catalyst exhibits improved kinetics for oxygen reduction/evolution reaction.When applied to rechargeable aqueous ZABs,it achieves a significant peak power density of 249.1 mW·cm^(-2).As binder-free air electrode catalyst,the flexible ZAB also displays stable cycling over 500 cycles with a minimal voltage gap of 0.42 V,showcasing promising applications in flexible electronic devices.
